Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius

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Autores:
Cruz Ruiz, Sergio Andres
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
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oai:repositorio.unal.edu.co:unal/81344
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/81344
https://repositorio.unal.edu.co/
Palabra clave:
630 - Agricultura y tecnologías relacionadas
580 - Plantas
Phaseolus acutifolius
Tolerancia al calor
Frijol
Adaptación
Introgresión
P. acutifolius
GWAS
QTL
P. vulgaris
Resistencia a altas temperaturas
Introgresiones
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_4898200ae304e8f3c28834ff8ae10572
oai_identifier_str oai:repositorio.unal.edu.co:unal/81344
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
dc.title.translated.eng.fl_str_mv Identification of QTLs associated with heat stress resistance using interspecific common bean populations derived from Phaseolus acutifolius.
title Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
spellingShingle Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
630 - Agricultura y tecnologías relacionadas
580 - Plantas
Phaseolus acutifolius
Tolerancia al calor
Frijol
Adaptación
Introgresión
P. acutifolius
GWAS
QTL
P. vulgaris
Resistencia a altas temperaturas
Introgresiones
title_short Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
title_full Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
title_fullStr Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
title_full_unstemmed Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
title_sort Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifolius
dc.creator.fl_str_mv Cruz Ruiz, Sergio Andres
dc.contributor.advisor.none.fl_str_mv Beebe, Steve
dc.contributor.author.none.fl_str_mv Cruz Ruiz, Sergio Andres
dc.contributor.educationalvalidator.none.fl_str_mv López Diana Carolina
dc.subject.ddc.spa.fl_str_mv 630 - Agricultura y tecnologías relacionadas
580 - Plantas
topic 630 - Agricultura y tecnologías relacionadas
580 - Plantas
Phaseolus acutifolius
Tolerancia al calor
Frijol
Adaptación
Introgresión
P. acutifolius
GWAS
QTL
P. vulgaris
Resistencia a altas temperaturas
Introgresiones
dc.subject.agrovoc.none.fl_str_mv Phaseolus acutifolius
Tolerancia al calor
Frijol
Adaptación
Introgresión
dc.subject.proposal.eng.fl_str_mv P. acutifolius
GWAS
QTL
P. vulgaris
dc.subject.proposal.spa.fl_str_mv Resistencia a altas temperaturas
Introgresiones
description archivo digital en formato PDF que contiene texto e imagenes en las 140 paginas que compone el documento
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-03-23T21:34:35Z
dc.date.available.none.fl_str_mv 2022-03-23T21:34:35Z
dc.date.issued.none.fl_str_mv 2022-03-17
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/81344
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/81344
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Beebe, Stevea44baa141f8642459af59ce9a2136ac5Cruz Ruiz, Sergio Andres4b70c11e201acd401a90bd0444d8f18dLópez Diana Carolina2022-03-23T21:34:35Z2022-03-23T21:34:35Z2022-03-17https://repositorio.unal.edu.co/handle/unal/81344Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/archivo digital en formato PDF que contiene texto e imagenes en las 140 paginas que compone el documentoIlustraciones, tablasVarios estudios han demostrado que Phaseolus acutifolius A. Gray es una fuente potencial de genes asociados a la tolerancia al calor que pueden ser utilizados para mejorar la adaptación del fríjol común (P. vulgaris L.) a las condiciones de alta temperatura, sin embargo, hasta ahora la base genética de esta resistencia es desconocida por ello se construyó una población de mapeo genético interespecífica entre P. acutifolius A. Gray y P. vulgaris L. con la cual se evaluaron componentes de rendimiento bajo condiciones controladas de alta temperatura (/25°C dia/noche, respectivamente). La población de mapeo genético se secuenció mediante el método de genotipado por secuenciación (Genotyping By sequencing, GBS), posteriormente se realizó un análisis de asociación genética con dos modelos de asociación genética para delimitar las regiones genómicas candidatas asociadas con la resistencia al estrés por calor encontrándose 31 asociaciones significativas para las variables: número de vainas, número de semillas por planta, peso promedio de semillas, índice de cosecha de vaina, número de vainas vanas por planta y rendimiento por planta. Se encontraron asociaciones que presentaron un efecto positivo y provinieron de los parentales silvestres de P. acutifolius A. Gray. Los genes presentes en las asociaciones significativas se relacionaron con la respuesta canónica al estrés por calor y a la señalización con fitohormonas como las auxinas y el etileno. (Texto tomado de la fuente)Several studies have shown that Phaseolus actifolius A. Gray is a potential source of genes associated with heat tolerance that can be used to improve the adaptation of common bean (P. vulgaris L.) to high temperature conditions, however, so far the genetic basis of this resistance is still unknown, therefore an interspecific genetic mapping population was constructed between P. acutifolius A. Gray and P. vulgaris L. to evaluate yield components under high temperature conditions. The genetic mapping population was sequenced using the Genotyping By sequencing (GBS) method, then a genetic association analysis was performed with the mixed linear models to delimit candidate genomic regions associated with resistance to heat stress, finding significant associations for the variables: number of pods and yield per plant that were associated with a positive effect came from the wild parents of P. acutifolius A. Gray. The genes present in the significant associations were related to the canonical response to heat stress and to the signaling that may be involved in the expression of these genes.Agencia Noruega para la Cooperación al Desarrollo (NORAD) que a través de la organización internacional Crop Trust financió este proyectoMaestríaMagíster en Ciencias AgrariasSe construyó una población de mapeo genético interespecífica en donde se incorporó P. vugaris y P. acutifolius mediante el uso de la línea puente VAP 1 (Barrera et al., 2018). Los fundadores de la población fueron: la línea puente VAP 1, dos accesiones silvestres de P. acutifolius A. Gray: G40056 y G40287 y cinco líneas comerciales de frijol común que se sabe a priori presentan algún grado de tolerancia a la sequía y con diferentes clases comerciales de grano: ICTA Ligero, SMC 214, SMR 155, SEF 10 y SEN 118 (Figura 2-1). El esquema de cruzamiento consistió en tomar polen de P. acutifolius y polinizar flores de VAP 1 vulgaris, su descendencia posteriormente fue cruzada una vez o dos veces con un parental P. vulgaris. Se realizaron catorce cruzamientos diferentes constituyendo la generación F1.2 compuesta por 50 semillas las cuales fueron sembradas en casa de malla y posteriormente incrementadas mediante selección individual hasta la generación F4.5 en campo. Los criterios de selección en campo fueron al azar para no favorecer un cierto tipo de genes en la población sin antes evaluarse en condiciones de calor. Se obtuvieron un total de 892 familias F4.5 que fueron evaluadas en un ensayo sin replicación en invernaderos climatizados donde se mantuvo la temperatura nocturna por encima de los 25°C. Con esta información se seleccionaron de forma individual 302 familias F5.6 muestreando de forma equitativa cada cruzamiento y garantizando la selección de familias contrastantes en cuanto a su desempeño en calorFitomejoramientovii, 89 páginas + anexosapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ciencias Agropecuarias - Maestría en Ciencias AgrariasFacultad de Ciencias AgrariasPalmiraUniversidad Nacional de Colombia - Sede Palmira630 - Agricultura y tecnologías relacionadas580 - PlantasPhaseolus acutifoliusTolerancia al calorFrijolAdaptaciónIntrogresiónP. acutifoliusGWASQTLP. vulgarisResistencia a altas temperaturasIntrogresionesIdentificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifoliusIdentification of QTLs associated with heat stress resistance using interspecific common bean populations derived from Phaseolus acutifolius.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlqudah, A. 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Bioinformatics, 28(24), 3326–3328. https://doi.org/10.1093/bioinformatics/bts606Identificación de QTLs asociados a la resistencia al estrés por calor usando poblaciones de fríjol común interespecíficas derivadas de Phaseolus acutifoliusCrop TrustInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81344/2/license.txt8153f7789df02f0a4c9e079953658ab2MD52ORIGINALTesis_Vulgaris_SAC.pdfTesis_Vulgaris_SAC.pdfapplication/pdf8519940https://repositorio.unal.edu.co/bitstream/unal/81344/1/Tesis_Vulgaris_SAC.pdf45d25c2fa074770e0c571ef8f8caebb2MD51THUMBNAILTesis_Vulgaris_SAC.pdf.jpgTesis_Vulgaris_SAC.pdf.jpgGenerated Thumbnailimage/jpeg5352https://repositorio.unal.edu.co/bitstream/unal/81344/3/Tesis_Vulgaris_SAC.pdf.jpg7d1ec3a2a5647fd30ae2790077d9220eMD53unal/81344oai:repositorio.unal.edu.co:unal/813442024-08-04 23:10:29.759Repositorio Institucional Universidad Nacional de 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EVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLiAqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4gCgpBbCBoYWNlciBjbGljIGVuIGVsIHNpZ3VpZW50ZSBib3TDs24sIHVzdGVkIGluZGljYSBxdWUgZXN0w6EgZGUgYWN1ZXJkbyBjb24gZXN0b3MgdMOpcm1pbm9zLiBTaSB0aWVuZSBhbGd1bmEgZHVkYSBzb2JyZSBsYSBsaWNlbmNpYSwgcG9yIGZhdm9yLCBjb250YWN0ZSBjb24gZWwgYWRtaW5pc3RyYWRvciBkZWwgc2lzdGVtYS4KClVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIC0gw5psdGltYSBtb2RpZmljYWNpw7NuIDE5LzEwLzIwMjEK

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