Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos

ilustraciones, graficas

Autores:: Navarro Saiz, Laura Marcela

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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oai_identifier_str	oai:repositorio.unal.edu.co:unal/81731
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos
dc.title.translated.eng.fl_str_mv	Evaluation of the presence and activity of cannabinoid receptors in dental pulp and human odontoblasts
title	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos
spellingShingle	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos 610 - Medicina y salud::617 - Cirugía, medicina regional, odontología, oftalmología, otología, audiología Pulpa Dental Receptores de Cannabinoides Dental Pulp Receptors, Cannabinoid Odontoblastos Dolor dental Receptor cannabinoide 1 (CB1) Receptor cannabinoide 2 (CB2) Pulpa dental Odontoblasts Dental pain Cannabinoid receptor 1 (CB1) Cannabinoid receptor 2 (CB2) Dental pulp
title_short	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos
title_full	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos
title_fullStr	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos
title_full_unstemmed	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos
title_sort	Evaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanos
dc.creator.fl_str_mv	Navarro Saiz, Laura Marcela
dc.contributor.advisor.none.fl_str_mv	Castellanos Parra, Jaime Eduardo Bernal Cepeda, Lilia Jadith
dc.contributor.author.none.fl_str_mv	Navarro Saiz, Laura Marcela
dc.contributor.researchgroup.spa.fl_str_mv	IBAPO
dc.subject.ddc.spa.fl_str_mv	610 - Medicina y salud::617 - Cirugía, medicina regional, odontología, oftalmología, otología, audiología
topic	610 - Medicina y salud::617 - Cirugía, medicina regional, odontología, oftalmología, otología, audiología Pulpa Dental Receptores de Cannabinoides Dental Pulp Receptors, Cannabinoid Odontoblastos Dolor dental Receptor cannabinoide 1 (CB1) Receptor cannabinoide 2 (CB2) Pulpa dental Odontoblasts Dental pain Cannabinoid receptor 1 (CB1) Cannabinoid receptor 2 (CB2) Dental pulp
dc.subject.other.spa.fl_str_mv	Pulpa Dental Receptores de Cannabinoides
dc.subject.other.eng.fl_str_mv	Dental Pulp Receptors, Cannabinoid
dc.subject.proposal.spa.fl_str_mv	Odontoblastos Dolor dental Receptor cannabinoide 1 (CB1) Receptor cannabinoide 2 (CB2) Pulpa dental
dc.subject.proposal.eng.fl_str_mv	Odontoblasts Dental pain Cannabinoid receptor 1 (CB1) Cannabinoid receptor 2 (CB2) Dental pulp
description	ilustraciones, graficas
publishDate	2022
dc.date.accessioned.none.fl_str_mv	2022-07-22T19:43:02Z
dc.date.available.none.fl_str_mv	2022-07-22T19:43:02Z
dc.date.issued.none.fl_str_mv	2022-07-05
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/81731
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/81731 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Castellanos Parra, Jaime Eduardod005422b5147ff1af3f046ffaf4328e6Bernal Cepeda, Lilia Jadith77fe9bc1954046f69b1bd6a7658a0f27Navarro Saiz, Laura Marcelaf97fae205729550e65e2a32eb4d54b4bIBAPO2022-07-22T19:43:02Z2022-07-22T19:43:02Z2022-07-05https://repositorio.unal.edu.co/handle/unal/81731Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, graficasLa inflamación, la sensibilidad y el dolor dental continúan siendo un reto en la práctica odontológica, es por esa razón que la búsqueda de alternativas farmacológicas para su control es una necesidad. En ese sentido los receptores cannabinoides CB1 y CB2 resultan una alternativa para ser utilizados como nuevos blancos terapéuticos. Dado que no se encontró evidencia en la literatura de su presencia en la pulpa dental y en odontoblastos en condiciones de inflamación, este trabajo de tesis tuvo como objetivo evaluar su expresión y funcionalidad en esas condiciones. Este trabajo está dividido en 4 capítulos: en el Capítulo 1 se realizó una revisión bibliográfica en la que se exploró la fisiología de los cannabinoides y de ambos receptores. Dado que no se encontró evidencia de la validación de genes de referencia en odontoblastos humanos en contexto inflamatorio, en el Capítulo 2 se describió una estrategia metodológica para la elección de los genes de referencia más adecuados para la realización de la cuantificación relativa de los receptores cannabinoides por medio de RT-qPCR. En el siguiente Capítulo, se comparó la presencia y actividad de receptores cannabinoides en odontoblastos humanos y en fibroblastos gingivales ya que ambas células tienen un papel importante en la modulación de la inflamación y el dolor en pulpitis y periodontitis. Finalmente, en el Capítulo 4 se evalúa la presencia de CB1 y CB2 en pulpas dentales y la presencia y actividad en cultivo de odontoblastos humanos en condiciones de inflamación. Esto permitió aportar luces sobre el papel de los receptores cannabinoides en el dolor e inflamación en odontología, y además de comprobar su expresión y modulación en células y tejidos dentales, abriendo paso a nuevas investigaciones que usen agonistas de ambos receptores para el control del dolor y la inflamación en encía y pulpa dental. (Texto tomado de la fuente)Inflammation, sensitivity and dental pain continue to be a challenge in dental practice, which is why the search for pharmacological alternatives for their control is a necessity. In this sense, CB1 and CB2 cannabinoid receptors are an alternative to be used as new therapeutic targets. Since no evidence was found in the literature of their presence in dental pulp and odontoblasts under inflammatory conditions, the aim of this thesis was to evaluate their expression and functionality under these conditions. This work is divided into 4 chapters: in Chapter 1, a literature review was conducted in which the physiology of cannabinoids and both receptors was explored. Since no evidence was found for the validation of reference genes in human odontoblasts in an inflammatory context, Chapter 2 described a methodological strategy for the choice of the most suitable reference genes for the relative quantification of cannabinoid receptors by RT-qPCR. In the next chapter, the presence and activity of cannabinoid receptors in human odontoblasts and gingival fibroblasts were compared as both cells play an important role in the modulation of inflammation and pain in pulpitis and periodontitis. Finally, Chapter 4 evaluates the presence of CB1 and CB2 in dental pulps and the presence and activity in cultured human odontoblasts under inflammatory conditions. This allowed us to shed light on the role of cannabinoid receptors in pain and inflammation in dentistry, and also to verify their expression and modulation in dental cells and tissues, opening the way to new research using agonists of both receptors for the control of pain and inflammation in gingiva and dental pulp.MaestríaMagíster en OdontologíaEstudio experimental in vitro cuantitativo y semicuantitativo. Se utilizarán células similares a odontoblastos y cortes histológicos de dientes sanos y cariados, se evaluará la expresión de receptores cannabinoides CB1 y CB2 por medio de RT- qPCR. En los cortes histológicos se realizarán inmunoperoxidasas e inmunofluorescencias con el fin de determinar la presencia y localización de los receptores en pulpa dental sana e inflamada. La actividad in vitro, sobre OLC de CB1 y CB2 se determinará usando la sonda para calcio Fluo-4, haciendo tratamiento con diferentes agonistas y antagonistas para evaluar los influjos de calcio y de esta manera evaluar su funcionalidad. Finalmente se realizarán los análisis estadísticos de las réplicas de cada experimento para determinar las significancias estadísticas para el posterior análisis de resultados. Con los datos obtenidos se podrá evaluar si los OLC y la pulpa dental humana expresan estos receptores y si son funcionales, además se determinará si existen cambios en los patrones de expresión de los receptores en dientes cariados y no cariados.Fisiopatología del dolor pulpar84 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Odontología - Maestría en OdontologíaFacultad de OdontologíaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::617 - Cirugía, medicina regional, odontología, oftalmología, otología, audiologíaPulpa DentalReceptores de CannabinoidesDental PulpReceptors, CannabinoidOdontoblastosDolor dentalReceptor cannabinoide 1 (CB1)Receptor cannabinoide 2 (CB2)Pulpa dentalOdontoblastsDental painCannabinoid receptor 1 (CB1)Cannabinoid receptor 2 (CB2)Dental pulpEvaluación de la presencia y actividad de receptores cannabinoides en pulpa dental y odontoblastos humanosEvaluation of the presence and activity of cannabinoid receptors in dental pulp and human odontoblastsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMSessle BJ. Peripheral and central mechanisms of orofacial inflammatory pain. . Translating Mechanisms Orofacial Neurological Disorder, 2011:179-206.Bae YC YA. Morphological fundamentals of pain processing in the dental pulp. J Oral Sci. 2020 2020;62(2):126-130.Maurin JC, Couble ML, Thivichon-Prince B, Magloire H. odontoblaste - Un acteur incontournable de la perception de la douleur dentinaire. Med Sci 2013;29(3):293-299.Lee K, Lee BM, Park CK, Kim YH, Chung G. Ion Channels Involved in Tooth Pain. Int J Mol Sci 2019;20(9):2266.Chung G, Jung SJ, Oh SB. Cellular and molecular mechanisms of dental nociception. J Dent Res 2013;92(11):948-55. .Solé-Magdalena A, Martínez-Alonso M, Coronado CA, Junquera LM, Cobo J, Vega JA. Molecular basis of dental sensitivity: The odontoblasts are multisensory cells and express multifunctional ion channels. . Ann Anat. 2018;215:20-29.Baldión PA, Velandia-Romero ML, Castellanos JE. 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Current Understanding of Cannabinoids and Detrusor Overactivity. Curr Bladder Dysfunct Rep 2017 Mar 7,;12(1):86-94.Biominerales PharmaInvestigadoresORIGINALDocumento tesis Laura M Navarro-Saiz.pdfDocumento tesis Laura M Navarro-Saiz.pdfTesis de Maestría en Odontologíaapplication/pdf1068094https://repositorio.unal.edu.co/bitstream/unal/81731/1/Documento%20tesis%20Laura%20M%20Navarro-Saiz.pdf9da7f5c95614db989462f2ebd9dc6a4dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81731/2/license.txt8153f7789df02f0a4c9e079953658ab2MD52THUMBNAILDocumento tesis Laura M Navarro-Saiz.pdf.jpgDocumento tesis Laura M Navarro-Saiz.pdf.jpgGenerated Thumbnailimage/jpeg3774https://repositorio.unal.edu.co/bitstream/unal/81731/3/Documento%20tesis%20Laura%20M%20Navarro-Saiz.pdf.jpg1c056af1cae4b2d718de586b3d3dca7bMD53unal/81731oai:repositorio.unal.edu.co:unal/817312023-08-06 23:03:41.569Repositorio Institucional Universidad Nacional de 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