Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa
Ilustraciones
- Autores:
-
Nova López, Carlos Julio
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/85444
- Palabra clave:
- 570 - Biología
580 - Plantas
630 - Agricultura y tecnologías relacionadas
Papa (Solanum tuberosum subsp. andígena var. pastusa suprema)
Cultivo in vitro
ADN recombinante
Proteínas recombinantes
factor de crecimiento epidérmico humano recombinante (rhEGF)
Cultivo in vitro
Solanum tuberosum
Agrobacterium tumefaciens
Recombinant proteins
in vitro culture
Solanum tuberosum
Agrobacterium tumefaciens
Recombinant human epidermal growth factor (rhEGF)
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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oai:repositorio.unal.edu.co:unal/85444 |
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa |
| dc.title.translated.eng.fl_str_mv |
Advances in the establishment of a platform for the production of recombinant human epidermal growth factor (rhEGF) using in vitro potato cultures |
| title |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa |
| spellingShingle |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa 570 - Biología 580 - Plantas 630 - Agricultura y tecnologías relacionadas Papa (Solanum tuberosum subsp. andígena var. pastusa suprema) Cultivo in vitro ADN recombinante Proteínas recombinantes factor de crecimiento epidérmico humano recombinante (rhEGF) Cultivo in vitro Solanum tuberosum Agrobacterium tumefaciens Recombinant proteins in vitro culture Solanum tuberosum Agrobacterium tumefaciens Recombinant human epidermal growth factor (rhEGF) |
| title_short |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa |
| title_full |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa |
| title_fullStr |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa |
| title_full_unstemmed |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa |
| title_sort |
Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papa |
| dc.creator.fl_str_mv |
Nova López, Carlos Julio |
| dc.contributor.advisor.none.fl_str_mv |
Arango Isaza, Rafael Eduardo Torres Bonilla, Javier Mauricio |
| dc.contributor.author.none.fl_str_mv |
Nova López, Carlos Julio |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biotecnología Vegetal UNALMED-CIB |
| dc.contributor.orcid.spa.fl_str_mv |
0000-0003-3128-4539 |
| dc.contributor.cvlac.spa.fl_str_mv |
https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001583298 |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología 580 - Plantas 630 - Agricultura y tecnologías relacionadas |
| topic |
570 - Biología 580 - Plantas 630 - Agricultura y tecnologías relacionadas Papa (Solanum tuberosum subsp. andígena var. pastusa suprema) Cultivo in vitro ADN recombinante Proteínas recombinantes factor de crecimiento epidérmico humano recombinante (rhEGF) Cultivo in vitro Solanum tuberosum Agrobacterium tumefaciens Recombinant proteins in vitro culture Solanum tuberosum Agrobacterium tumefaciens Recombinant human epidermal growth factor (rhEGF) |
| dc.subject.agrovoc.none.fl_str_mv |
Papa (Solanum tuberosum subsp. andígena var. pastusa suprema) |
| dc.subject.lemb.none.fl_str_mv |
Cultivo in vitro ADN recombinante |
| dc.subject.proposal.spa.fl_str_mv |
Proteínas recombinantes factor de crecimiento epidérmico humano recombinante (rhEGF) Cultivo in vitro Solanum tuberosum Agrobacterium tumefaciens |
| dc.subject.proposal.eng.fl_str_mv |
Recombinant proteins in vitro culture Solanum tuberosum Agrobacterium tumefaciens Recombinant human epidermal growth factor (rhEGF) |
| description |
Ilustraciones |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023-05-02 |
| dc.date.accessioned.none.fl_str_mv |
2024-01-25T15:53:34Z |
| dc.date.available.none.fl_str_mv |
2024-01-25T15:53:34Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/85444 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/85444 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.indexed.spa.fl_str_mv |
LaReferencia |
| dc.relation.references.spa.fl_str_mv |
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Universidad Nacional de Colombia |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Arango Isaza, Rafael Eduardob7e61322453072ebf7408a0f587e39c1600Torres Bonilla, Javier Mauricio315aa45692339fe7f1e42ff1588fbab0600Nova López, Carlos Julio8541b76b58facc8a2e703641a81fe2e0Biotecnología Vegetal UNALMED-CIB0000-0003-3128-4539https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=00015832982024-01-25T15:53:34Z2024-01-25T15:53:34Z2023-05-02https://repositorio.unal.edu.co/handle/unal/85444Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/IlustracionesLas proteínas recombinantes terapéuticas constituyen la base para el tratamiento de muchas de las enfermedades complejas que aquejan a la humanidad, por lo que se han posicionado como el sector de mayor crecimiento dentro de la industria farmacéutica. Tradicionalmente los cultivos bacterianos, de levaduras y de células de mamífero se han utilizado como plataformas de producción de estos medicamentos, pero en los últimos años los cultivos de plantas y de células vegetales han emergido como una nueva alternativa de expresión, con incluso casos de medicamentos que han alcanzado fases avanzadas de desarrollo o de mercado. El objetivo de este trabajo fue avanzar en el proceso de desarrollo de una plataforma basada en el uso de cultivos in vitro de papa (Solanum tuberosum subsp. andígena var. pastusa suprema) que permita en un futuro la producción heteróloga del factor de crecimiento epidérmico humano (hEGF), una proteína con importantes aplicaciones terapéuticas y cosméticas. Para esto, se presentan aportes en aspectos relacionados con: i) el establecimiento de cultivos de plantas de papa no transformadas, ii) el diseño in silico y la síntesis de casetes genéticos para la expresión del hEGF, iii) la obtención de cepas de Agrobacterium tumefaciens transformadas con los vectores de expresión y iv) la ejecución de ensayos preliminares de transformación genética para la obtención de líneas de plantas transformadas con el gen hEGF. De manera particular, se propone la inducción de la brotación de tubérculos de papa con el uso de GA3 y la incubación de las semillas a 26 0C bajo fotoperiodo natural como estrategia para la obtención del material de partida requerido para el desarrollo de los cultivos no transformados. No se encontraron diferencias significativas en los porcentajes de desinfección de los brotes cuando se usaron hipoclorito de sodio o bicloruro de mercurio como agentes de desinfección, aunque el porcentaje de supervivencia de los explantes se vio favorecido con el uso de hipoclorito. Se diseñaron dos casetes de expresión que, además de contener la secuencia hEGF con el uso codónico optimizado para la expresión en papa, incluyen también secuencias promotoras (CaMV 35S 2x), terminadoras (Pin II) y otros elementos regulatorios (secuencia 5’ UTR, secuencia de señalización, secuencia de retención, colas Hisx6 y sitios de unión a la matriz extracelular) seleccionados estratégicamente con el fin de garantizar elevados niveles de expresión, producción y purificación de la proteína. Los casetes se clonaron en el vector pCAMBIA 2300 y se introdujeron, previa confirmación por secuenciación, en cepas de Agrobacterium tumfaciens EHA 105 y LBA4400, lo que permitió la obtención de colonias transformantes que se confirmaron por PCR y que fueron empleadas en ensayos preliminares de infección de hojas y entrenudos con el fin de evaluar la formación de callo y la eventual regeneración de plantas transformadas. Aunque el porcentaje de formación de callo en los explantes de hoja sometidos a infección fue bajo y aunque se observó una pobre capacidad regenerativa en dos tipos de explante utilizados, los avances obtenidos sirven de insumo para el desarrollo de investigaciones futuras que tengan por objetivo principal el desarrollo de cultivos transgénicos de papa y la evaluación de la producción del rhEGF en esta plataforma vegetal. (texto tomado de la fuente)Therapeutic recombinant proteins are the basis for the treatment of many complex diseases that afflict humanity, making them the fastest-growing sector in the pharmaceutical industry. Bacterial, yeast, and mammalian cell cultures have traditionally been used as production platforms for these medicines, but in recent years, plant and plant cell cultures have emerged as a new expression alternative, with even cases of drugs that have reached advanced stages of development or market. The objective of this work was to advance the development process of a platform based on the use of in vitro potato cultures (Solanum tuberosum subsp. andígena var. pastusa suprema) that will allow for future heterologous production of human epidermal growth factor (hEGF), a protein with important therapeutic and cosmetic applications. Contributions are presented in aspects related to: i) the establishment of non-transformed potato plant cultures, ii) in silico design and genetic cassette synthesis for hEGF expression, iii) the generation of transformed Agrobacterium tumefaciens strains with expression vectors, and iv) preliminary genetic transformation assays to obtain transformed plant lines with the hEGF gene. Particularly, the induction of potato tuber sprouting using GA3 and seed incubation at 26 0C under natural photoperiod is proposed as a strategy to obtain the required starting material for non-transformed culture development. There were no significant differences in disinfection percentages of the shoots when sodium hypochlorite or mercuric bichloride were used as disinfection agents, although the explants' survival rate was favored with the use of hypochlorite. Two expression cassettes were designed that, in addition to containing the hEGF sequence with optimized codon usage for expression in potato, also include promoter (CaMV 35S 2x), terminator (Pin II), and other regulatory elements (5’ UTR sequence, signaling sequence, retention sequence, Hisx6 tails, and extracellular matrix binding sites) strategically selected to guarantee high expression levels, production, and purification of the protein. The cassettes were cloned into the pCAMBIA 2300 vector and introduced, after confirmation by sequencing, into Agrobacterium tumfaciens EHA 105 and LBA4400 strains, allowing the obtaining of transforming colonies confirmed by PCR and used in preliminary infection assays of leaves and internodes to evaluate callus formation and eventual regeneration of transformed plants. Although the percentage of callus formation in infected leaf explants was low, and poor regenerative capacity was observed in two types of explants used, the advances obtained serve as input for future research aimed at developing transgenic potato cultures and evaluating rhEGF production on this plant platformMaestríaMagíster en Ciencias AgrariasÁrea Curricular en Producción Agraria Sostenible121 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias Agrarias - Maestría en Ciencias AgrariasFacultad de Ciencias AgrariasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín570 - Biología580 - Plantas630 - Agricultura y tecnologías relacionadasPapa (Solanum tuberosum subsp. andígena var. pastusa suprema)Cultivo in vitroADN recombinanteProteínas recombinantesfactor de crecimiento epidérmico humano recombinante (rhEGF)Cultivo in vitroSolanum tuberosumAgrobacterium tumefaciensRecombinant proteinsin vitro cultureSolanum tuberosumAgrobacterium tumefaciensRecombinant human epidermal growth factor (rhEGF)Avances en el establecimiento de una plataforma para la producción del factor de crecimiento epidérmico humano recombinante (rhEGF) utilizando cultivos in vitro de papaAdvances in the establishment of a platform for the production of recombinant human epidermal growth factor (rhEGF) using in vitro potato culturesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMLaReferenciaAbrahamian, P., Hammond, R. 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Molecular Biotechnology, 60(6), 387-395. https://doi.org/10.1007/s12033-018-0075-6EstudiantesInvestigadoresPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85444/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1152189450.2023.pdf1152189450.2023.pdfTesis de Maestría en Ciencias Agrariasapplication/pdf2860570https://repositorio.unal.edu.co/bitstream/unal/85444/2/1152189450.2023.pdfe8932efd9bb176158b96b36ddd8ca7aeMD52THUMBNAIL1152189450.2023.pdf.jpg1152189450.2023.pdf.jpgGenerated Thumbnailimage/jpeg5737https://repositorio.unal.edu.co/bitstream/unal/85444/3/1152189450.2023.pdf.jpga3e9edada1a770cb68e8ef3079caee28MD53unal/85444oai:repositorio.unal.edu.co:unal/854442024-08-21 23:13:03.575Repositorio Institucional Universidad Nacional de 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