Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation
Agrichemical involve health risks for producers and consumers; they can also affect beneficial insects, birds, fish and mammals. Bacillus thuringiensis-based biopesticides specific for insect plagues are available, which, represent an alternative to traditional chemically-based products. Culture med...
- Autores:
-
Escobar, Jenny M.
Pardo, E. Milena
Buitrago, Gustavo
López, Luis A.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2004
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/22128
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/22128
http://bdigital.unal.edu.co/13162/
http://bdigital.unal.edu.co/13162/1/
- Palabra clave:
- Biopesticides
culture media
surface response
dual response
contour plots
central composite design
biopesticidas
medios de cultivo
superficie de respuesta
respuesta dual
gráficas de contorno
diseño compuesto central
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| id |
UNACIONAL2_43577aa8e278e8ca86f3811c5ad5b8f6 |
|---|---|
| oai_identifier_str |
oai:repositorio.unal.edu.co:unal/22128 |
| network_acronym_str |
UNACIONAL2 |
| network_name_str |
Universidad Nacional de Colombia |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation |
| title |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation |
| spellingShingle |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation Biopesticides culture media surface response dual response contour plots central composite design biopesticidas medios de cultivo superficie de respuesta respuesta dual gráficas de contorno diseño compuesto central |
| title_short |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation |
| title_full |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation |
| title_fullStr |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation |
| title_full_unstemmed |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation |
| title_sort |
Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation |
| dc.creator.fl_str_mv |
Escobar, Jenny M. Pardo, E. Milena Buitrago, Gustavo López, Luis A. |
| dc.contributor.author.spa.fl_str_mv |
Escobar, Jenny M. Pardo, E. Milena Buitrago, Gustavo López, Luis A. |
| dc.subject.proposal.spa.fl_str_mv |
Biopesticides culture media surface response dual response contour plots central composite design biopesticidas medios de cultivo superficie de respuesta respuesta dual gráficas de contorno diseño compuesto central |
| topic |
Biopesticides culture media surface response dual response contour plots central composite design biopesticidas medios de cultivo superficie de respuesta respuesta dual gráficas de contorno diseño compuesto central |
| description |
Agrichemical involve health risks for producers and consumers; they can also affect beneficial insects, birds, fish and mammals. Bacillus thuringiensis-based biopesticides specific for insect plagues are available, which, represent an alternative to traditional chemically-based products. Culture media design is an impor-tant factor in the production of biopesticides based on Bacillus thuringiensis. The media have to be economi-cal and they must contain all the nutrition factors required by the bacteria. The yield of active ingredient, comprising the toxin-containing crystal protein and bacterial spores, must be high and with adequate toxi-city to formulate the biopesticide. In this work we studied different culture media and optimised a promi-sing culture medium for B. thuringiensis fermentation to obtain an active ingredient for use as raw material in manufacturing a biopesticide. The final concentration of active ingredient was 15 to 16 g/L and the raw material cost around US $0,30/kg product. Calculations for the experimental phase were done using the response surface technique in a central composite design (CCD), the optimisation phase used Derringer and Suich (1980) method for dual response. Key words: Biopesticides, culture media, surface response, dual response, contour plots, central composite design. |
| publishDate |
2004 |
| dc.date.issued.spa.fl_str_mv |
2004 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-25T20:33:23Z |
| dc.date.available.spa.fl_str_mv |
2019-06-25T20:33:23Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/22128 |
| dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/13162/ http://bdigital.unal.edu.co/13162/1/ |
| url |
https://repositorio.unal.edu.co/handle/unal/22128 http://bdigital.unal.edu.co/13162/ http://bdigital.unal.edu.co/13162/1/ |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/biotecnologia/article/view/525 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología Revista Colombiana de Biotecnología |
| dc.relation.ispartofseries.none.fl_str_mv |
Revista Colombiana de Biotecnología; Vol. 6, núm. 2 (2004); 43-53 1909-8758 0123-3475 |
| dc.relation.references.spa.fl_str_mv |
Escobar, Jenny M. and Pardo, E. Milena and Buitrago, Gustavo and López, Luis A. (2004) Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation. Revista Colombiana de Biotecnología; Vol. 6, núm. 2 (2004); 43-53 1909-8758 0123-3475 . |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
| dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia |
| institution |
Universidad Nacional de Colombia |
| bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/22128/1/525-3591-2-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/22128/2/525-3592-2-PB.doc https://repositorio.unal.edu.co/bitstream/unal/22128/3/525-3591-2-PB.pdf.jpg |
| bitstream.checksum.fl_str_mv |
1346f3d72ca8f539a489972a52e51081 ea2de8a97a189475d7e7126fb7351268 a68b33fb1071448e40f6564cabf83412 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| repository.mail.fl_str_mv |
repositorio_nal@unal.edu.co |
| _version_ |
1814090256946823168 |
| spelling |
Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Escobar, Jenny M.186430b3-031c-4691-bc7c-eabb57db51c5300Pardo, E. Milena52eafb02-9afd-4f6c-8720-6cb918cb3018300Buitrago, Gustavo62562275-5d43-4f51-8fdc-05035be2b227300López, Luis A.2551c19b-29b8-498c-8ad8-0f4bf4294a1e3002019-06-25T20:33:23Z2019-06-25T20:33:23Z2004https://repositorio.unal.edu.co/handle/unal/22128http://bdigital.unal.edu.co/13162/http://bdigital.unal.edu.co/13162/1/Agrichemical involve health risks for producers and consumers; they can also affect beneficial insects, birds, fish and mammals. Bacillus thuringiensis-based biopesticides specific for insect plagues are available, which, represent an alternative to traditional chemically-based products. Culture media design is an impor-tant factor in the production of biopesticides based on Bacillus thuringiensis. The media have to be economi-cal and they must contain all the nutrition factors required by the bacteria. The yield of active ingredient, comprising the toxin-containing crystal protein and bacterial spores, must be high and with adequate toxi-city to formulate the biopesticide. In this work we studied different culture media and optimised a promi-sing culture medium for B. thuringiensis fermentation to obtain an active ingredient for use as raw material in manufacturing a biopesticide. The final concentration of active ingredient was 15 to 16 g/L and the raw material cost around US $0,30/kg product. Calculations for the experimental phase were done using the response surface technique in a central composite design (CCD), the optimisation phase used Derringer and Suich (1980) method for dual response. Key words: Biopesticides, culture media, surface response, dual response, contour plots, central composite design.Los insecticidas químicos usados indiscriminadamente traen riesgos para la salud de quienes los aplican y de quienes consumen alimentos contaminados con éstos; además atacan insectos benéficos, aves, peces y mamíferos. Como alternativa al uso de insecticidas químicos están los bioinsecticidas, como es el caso de Bacillus thuringiensis, que es específico para el insecto plaga que se desea controlar. Un factor clave en la producción por fermentación de biopesticidas basados en Bacillus thuringiensis es el diseño del medio de cultivo, el cual debe ser económico y contener todos los nutrientes necesarios para el crecimiento del microorganismo. Del cultivo se espera que rinda una alta producción de ingrediente activo conformado por los cristales que contienen las toxinas y por la espora del microorganismo, y que este ingrediente posea el valor de toxicidad requerido para la formulación del producto comercial. En este trabajo se estudiaron diferentes medios de cultivo, se seleccionó un medio promisorio y se optimizó para la fermentación con una cepa nativa de B. thuringiensis teniendo en cuenta no sólo el ingrediente activo sino también los costos que éste genera en materias primas. Se lograron concentraciones finales de ingrediente activo entre 15 y 16 g/L con un costo aproximado por materias primas de $650/kg producto (aproximadamente US$0,30/kg producto). Para esto se utilizó la metodología de superficies de respuesta en un diseño compuesto central (DCC) para la fase de experimentación, y para la fase de optimización se utilizó el método desarrollado por Derringer y Suich (1980) para múltiples respuestas. Palabras clave: biopesticidas, medios de cultivo, superficie de respuesta, respuesta dual, gráficas de contorno, diseño compuesto central.application/pdfspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/biotecnologia/article/view/525Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de BiotecnologíaRevista Colombiana de BiotecnologíaRevista Colombiana de Biotecnología; Vol. 6, núm. 2 (2004); 43-53 1909-8758 0123-3475Escobar, Jenny M. and Pardo, E. Milena and Buitrago, Gustavo and López, Luis A. (2004) Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation. Revista Colombiana de Biotecnología; Vol. 6, núm. 2 (2004); 43-53 1909-8758 0123-3475 .Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentationArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTBiopesticidesculture mediasurface responsedual responsecontour plotscentral composite designbiopesticidasmedios de cultivosuperficie de respuestarespuesta dualgráficas de contornodiseño compuesto centralORIGINAL525-3591-2-PB.pdfapplication/pdf1463232https://repositorio.unal.edu.co/bitstream/unal/22128/1/525-3591-2-PB.pdf1346f3d72ca8f539a489972a52e51081MD51525-3592-2-PB.docapplication/msword359936https://repositorio.unal.edu.co/bitstream/unal/22128/2/525-3592-2-PB.docea2de8a97a189475d7e7126fb7351268MD52THUMBNAIL525-3591-2-PB.pdf.jpg525-3591-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg7672https://repositorio.unal.edu.co/bitstream/unal/22128/3/525-3591-2-PB.pdf.jpga68b33fb1071448e40f6564cabf83412MD53unal/22128oai:repositorio.unal.edu.co:unal/221282023-10-05 23:04:52.844Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |