Exploratory analysis for the optimization of culture media for bacillus thuringiensis fermentation
Agrichemical involve health risks for producers and consumers; they can also affect beneficial insects, birds, fish and mammals. Bacillus thuringiensis-based biopesticides specific for insect plagues are available, which, represent an alternative to traditional chemically-based products. Culture med...
- Autores:
-
Escobar, Jenny M.
Pardo, E. Milena
Buitrago, Gustavo
López, Luis A.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2004
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/22128
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/22128
http://bdigital.unal.edu.co/13162/
http://bdigital.unal.edu.co/13162/1/
- Palabra clave:
- Biopesticides
culture media
surface response
dual response
contour plots
central composite design
biopesticidas
medios de cultivo
superficie de respuesta
respuesta dual
gráficas de contorno
diseño compuesto central
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| Summary: | Agrichemical involve health risks for producers and consumers; they can also affect beneficial insects, birds, fish and mammals. Bacillus thuringiensis-based biopesticides specific for insect plagues are available, which, represent an alternative to traditional chemically-based products. Culture media design is an impor-tant factor in the production of biopesticides based on Bacillus thuringiensis. The media have to be economi-cal and they must contain all the nutrition factors required by the bacteria. The yield of active ingredient, comprising the toxin-containing crystal protein and bacterial spores, must be high and with adequate toxi-city to formulate the biopesticide. In this work we studied different culture media and optimised a promi-sing culture medium for B. thuringiensis fermentation to obtain an active ingredient for use as raw material in manufacturing a biopesticide. The final concentration of active ingredient was 15 to 16 g/L and the raw material cost around US $0,30/kg product. Calculations for the experimental phase were done using the response surface technique in a central composite design (CCD), the optimisation phase used Derringer and Suich (1980) method for dual response. Key words: Biopesticides, culture media, surface response, dual response, contour plots, central composite design. |
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