Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá

ilustraciones, graficas, mapas

Autores:
Gutiérrez Vásquez, Elizabeth
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/81775
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/81775
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::576 - Genética y evolución
Candida auris
Resistencia antimicóticos
Diagnóstico erróneo
Mutaciones
Antifungal resistance
Antifungal resistance
Mutations
Misdiagnosis
Hongos patógenos
Pathogenic fungi
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openAccess
License
Atribución-SinDerivadas 4.0 Internacional
id UNACIONAL2_40b53f16b53518cbb67e95f1944ffc63
oai_identifier_str oai:repositorio.unal.edu.co:unal/81775
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
dc.title.translated.eng.fl_str_mv Genetic characterization by next generation sequencing of Candida auris isolated from invasive infections in hospitals of Bogotá
title Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
spellingShingle Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
570 - Biología::576 - Genética y evolución
Candida auris
Resistencia antimicóticos
Diagnóstico erróneo
Mutaciones
Antifungal resistance
Antifungal resistance
Mutations
Misdiagnosis
Hongos patógenos
Pathogenic fungi
title_short Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
title_full Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
title_fullStr Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
title_full_unstemmed Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
title_sort Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de Bogotá
dc.creator.fl_str_mv Gutiérrez Vásquez, Elizabeth
dc.contributor.advisor.none.fl_str_mv Chala Palacios, María del Socorro
Pinzón Velasco, Andrés
dc.contributor.author.none.fl_str_mv Gutiérrez Vásquez, Elizabeth
dc.contributor.researchgroup.spa.fl_str_mv Grupo de Laboratorios de Salud pública (SDS)
dc.contributor.rightsholder.none.fl_str_mv Sandra Liliana Gómez
dc.subject.ddc.spa.fl_str_mv 570 - Biología::576 - Genética y evolución
topic 570 - Biología::576 - Genética y evolución
Candida auris
Resistencia antimicóticos
Diagnóstico erróneo
Mutaciones
Antifungal resistance
Antifungal resistance
Mutations
Misdiagnosis
Hongos patógenos
Pathogenic fungi
dc.subject.proposal.spa.fl_str_mv Candida auris
Resistencia antimicóticos
Diagnóstico erróneo
Mutaciones
dc.subject.proposal.eng.fl_str_mv Antifungal resistance
Antifungal resistance
Mutations
Misdiagnosis
dc.subject.unesco.none.fl_str_mv Hongos patógenos
Pathogenic fungi
description ilustraciones, graficas, mapas
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-08-03T20:51:52Z
dc.date.available.none.fl_str_mv 2022-08-03T20:51:52Z
dc.date.issued.none.fl_str_mv 2022
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/81775
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/81775
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv RedCol
LaReferencia
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dc.format.extent.spa.fl_str_mv xxii, 88 páginas
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dc.publisher.spa.fl_str_mv Universidad Nacional Colombia
dc.publisher.program.spa.fl_str_mv Bogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.publisher.department.spa.fl_str_mv Instituto de Biotecnología (IBUN)
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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spelling Atribución-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Chala Palacios, María del Socorroabfa67f1f7484fb0a1f25c2dce2e0acePinzón Velasco, Andrés5f85b4ad70276c20f06237027ad47a49Gutiérrez Vásquez, Elizabethb30e24291e905316e3774f21874515edGrupo de Laboratorios de Salud pública (SDS)Sandra Liliana Gómez2022-08-03T20:51:52Z2022-08-03T20:51:52Z2022https://repositorio.unal.edu.co/handle/unal/81775Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, graficas, mapasCandida auris es un hongo patógeno emergente y oportunista caracterizado por su difícil identificación con pruebas de diagnóstico convencionales, lo cual retrasa el tratamiento del paciente. Además, es común que éste patógeno presente resistencia a la primera línea de antimicóticos utilizados habitualmente en el tratamiento de infecciones por hongos (azoles, polienos y equinocandinas). Está resistencia ha sido asociada a mutaciones genómicas, las cuales también han sido encontradas en otras especies patógenas del género Candida. Por otro lado, la falta de información sobre su diversidad genética en la mayoría de aislamientos ha impedido estudiar a fondo el impacto de estas mutaciones y de la epidemiologia genómica de este microorganismo. En este estudio se buscó caracterizar aislamientos de C. auris enviados al laboratorio de salud pública de Bogotá como parte del programa de vigilancia epidemiológica. En un estudio descriptivo y de corte trasversal, 20 aislamientos obtenidos de hospitales de Bogotá fueron identificados como C. auris mediante técnicas fenotípicas y caracterizados molecularmente usando espectometría de masas maldiTOF y NGS (Secuenciación de Nueva Generación). La identificación de los 20 aislamientos se confirmó mediante análisis filogenéticos. La diversidad genética observada fue baja y sugirió la existencia del efecto de selección direccional o selección de fondo. Se identificaron cuatro genotipos por sus perfiles MLST que podrían optimizarse ya que no describeron la totalidad de la diversidad genética en los aislamientos bogotanos. Aunque no se encontraron mutaciones asociadas a resistencia, sí se encontraron duplicaciones en varios genes, incluyendo adp1, pmi1, zwF1b y se presentaron cambios de mutaciones parsimoniosas vps13, zwF1b, cdr1 y erg3. También se observaron sustitución de aminoácidos en algunos de estos genes: erg3 (H771R), erg11 (G459S) y rpn2 (K898E). Los análisis de diversidad genética y filogenéticos mostraron una baja diversidad genética en los 20 aislamientos bogotanos, todos estos agrupándose dentro del clado IV de Suramérica y presentando un número relativamente bajo de diferencias de SNP entre ellos, aunque lo suficientemente alto como para sugerir ausencia de clonalidad en estos aislamientos. Además, las desviaciones del modelo de neutralidad selectiva sugieren que la baja diversidad podría explicarse por un modelo de expansión de la población. A partir de estos hallazgos, se sugiere que C. auris ha tenido una trasmisión continua y generalizada en el territorio colombiano. (Texto tomado de la fuente)Candida auris is an emerging, opportunistic and pathogenic fungus characterized by its difficult identification through conventional diagnostic tests, thus delaying the treatment of the patient. Furthermore, it is usual for this pathogen to be resistant to the antifungals of first line utilized commonly for treating fungal infections (azoles, polyenes and echinocandins). This resistance has been associated to genomic mutations, which have also been found in other pathogenic species of Candida. On the other hand, the lack of information about the genetic diversity in the great majority of isolates has hampered to deeply study the impact of this mutations and the genomic epidemiology of this pathogen. This study aimed at characterizing C. auris isolates sent to the public health laboratory of Bogotá as part of an epidemiological surveillance program. In this descriptive and cross-sectional study, 20 isolates obtained from hospitals in Bogotá were identified as C. auris through phenotypic techniques and molecularly characterized using MALDI-TOF mass spectrometry and NGS (Next Generation Sequencing). The identification of the 20 isolates was confirmed by phylogenetic analysis. The genetic diversity observed was low and suggested the presence of effects of directional selection or background selection. Four genotypes were identified using their MLST profiles, as though this technique could be optimized since these profiles did not describe the complete genetic diversity presented by Bogota isolates. Although resistance-associated mutations were not found, duplications were found in several genes, including adp1, pmi1, zwF1b, and parsimonious mutation changes vps13, zwF1b, cdr1, and erg3. Some of these genes also presented the following amino acid substitutions: erg3 (H771R), erg11 (G459S), and rpn2 (K898E). The genetic diversity and phylogenetic analyzes showed a low genetic diversity in the 20 Bogotá isolates, all of them grouped within the clade IV of South America and presenting a relatively low number of SNP differences between them, although this number was high enough to suggest the absence of clonality in these isolates. Furthermore, the deviations from the model of selective neutrality suggest that a population expansion model could explain the low diversity. From these findings, it is suggested that C. auris has had a continuous and generalized transmission in the Colombian territory.El Laboratorio de Salud Pública de la Secretaría Distrital de Salud es una entidad pública, encargada del desarrollo de acciones técnico-administrativas realizadas en atención a las personas y a la salud ambiental con propósitos de vigilancia en salud pública, vigilancia y control sanitario, gestión de la calidad e investigación. Los ejes estratégicos sobre los cuales el Laboratorio de Salud Pública basa su gestión para orientar sus procesos y competencias son los siguientes: 1. Vigilancia en Salud Pública. Eje estratégico orientado al desarrollo de acciones para apoyar la vigilancia en salud pública y la vigilancia y control sanitario. 2. Gestión de la Calidad. Eje estratégico orientado al desarrollo de acciones para el mejoramiento progresivo en el cumplimiento de los estándares óptimos de calidad. 3. Prestación de Servicios. Eje estratégico orientado al desarrollo de acciones para el mejoramiento de la capacidad de oferta de servicios desde los laboratorios públicos y privados en los diferentes niveles territoriales. 4. Investigación. Eje estratégico orientado al desarrollo de acciones para apoyar la investigación desde el laboratorio y contribuir con el Sistema Nacional de Ciencia y Tecnología en el desarrollo de investigaciones en el área de la salud. El Laboratorio de Salud Pública tiene implementado un Sistema de Gestión de la Calidad bajo la norma NTC-ISO/IEC 17025:2017, a través del cual se demuestra la competencia técnica de las metodologías analíticas que realiza en las áreas de Vigilancia de Enfermedades de Eventos de Interés en Salud Pública y Vigilancia del Ambiente y el Consumo. Misión Atender las necesidades requeridas en el Distrito Capital, relacionadas con actividades de investigación, vigilancia y control en Salud Pública, asesoría, asistencia técnica y control de calidad a la Red Distrital de Laboratorios; a través de la realización de ensayos confiables y oportunos, soportados por personal profesional idóneo, comprometido y altamente calificado, enmarcados en un Sistema de Gestión de Calidad, con recursos, infraestructura adecuada y desarrollo tecnológico permanente. Visión Ser reconocido como modelo en desarrollo de procesos de gestión de calidad en laboratorios de salud pública y como un centro de alta tecnología con la realización de pruebas analíticas acreditadas bajo la norma ISO 17025:2017, que apoyen la investigación, vigilancia y control de eventos de interés en salud colectiva zonal, nacional e internacional, con el mejor servicio en asesoría, asistencia técnica, control de calidad y apoyo diagnóstico a la Red Distrital de laboratorios. Áreas del Laboratorio Por favor haga clic en cada uno de los siguientes enlaces: 1. Vigilancia de eventos de interés en salud pública – VEISP 2. Vigilancia y control sanitario – VCS 3. Gestión de la calidad 4. Epidemiología y sistemas de información 5. InvestigacionesMaestríaMagíster en Ciencias - MicrobiologíaBiología Molecular de Agentes Infecciososxxii, 88 páginasapplication/pdfspaUniversidad Nacional ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaInstituto de Biotecnología (IBUN)Facultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::576 - Genética y evoluciónCandida aurisResistencia antimicóticosDiagnóstico erróneoMutacionesAntifungal resistanceAntifungal resistanceMutationsMisdiagnosisHongos patógenosPathogenic fungiCaracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en hospitales de BogotáGenetic characterization by next generation sequencing of Candida auris isolated from invasive infections in hospitals of BogotáTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMRedColLaReferencia1. 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Emerging infectious diseases, 2019. 25(9): p. 1763-1765.Caracterización genética mediante secuenciación de siguiente generación de Candida auris aislada de infecciones invasivas en Hospitales de BogotáSecretaria Distrital de Salud de Bogotá Laboratorio de Salud PúblicaInvestigadoresMaestrosORIGINAL1010223360_2022.pdf1010223360_2022.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf4683235https://repositorio.unal.edu.co/bitstream/unal/81775/3/1010223360_2022.pdfaafea0edb3d66bfe9b722227bca05659MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81775/4/license.txt8153f7789df02f0a4c9e079953658ab2MD54THUMBNAIL1010223360_2022.pdf.jpg1010223360_2022.pdf.jpgGenerated Thumbnailimage/jpeg4314https://repositorio.unal.edu.co/bitstream/unal/81775/5/1010223360_2022.pdf.jpg34f6812ae905fcb7c8b72a5bd11a2042MD55unal/81775oai:repositorio.unal.edu.co:unal/817752024-08-07 23:10:54.117Repositorio Institucional Universidad Nacional de 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EVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLiAqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4gCgpBbCBoYWNlciBjbGljIGVuIGVsIHNpZ3VpZW50ZSBib3TDs24sIHVzdGVkIGluZGljYSBxdWUgZXN0w6EgZGUgYWN1ZXJkbyBjb24gZXN0b3MgdMOpcm1pbm9zLiBTaSB0aWVuZSBhbGd1bmEgZHVkYSBzb2JyZSBsYSBsaWNlbmNpYSwgcG9yIGZhdm9yLCBjb250YWN0ZSBjb24gZWwgYWRtaW5pc3RyYWRvciBkZWwgc2lzdGVtYS4KClVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIC0gw5psdGltYSBtb2RpZmljYWNpw7NuIDE5LzEwLzIwMjEK

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