Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant pro...
- Autores:
-
Criollo, Hernando
Perea, Margarita
Toribio, Mariano
Muñoz, Johanna
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2014
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/49740
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/49740
http://bdigital.unal.edu.co/43228/
- Palabra clave:
- Embryo culture
recalcitrance
plant growth regulators
tissue culture
plant propagation
fruit.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Criollo, Hernando5fa44036-f98e-49bc-acc8-a1da9e0b752a300Perea, Margaritae13f1753-10a9-4aaa-a399-ec776c0ed241300Toribio, Mariano26d0285f-5f49-4f61-93b9-6eeccd0e1cd6300Muñoz, Johanna99d27381-d9d5-4013-80a9-5cde8c1ef8d73002019-06-29T09:15:44Z2019-06-29T09:15:44Z2014-08-09https://repositorio.unal.edu.co/handle/unal/49740http://bdigital.unal.edu.co/43228/Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 μM of NAA were added to the medium with sucrose (50.0 and 263.1 μM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 μM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 μM of NAA + 263.1 μM of sucrose (32%).application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/43861Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965Criollo, Hernando and Perea, Margarita and Toribio, Mariano and Muñoz, Johanna (2014) Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.). Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965 .Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTEmbryo culturerecalcitranceplant growth regulatorstissue cultureplant propagationfruit.ORIGINAL43861-220916-1-PB.pdfapplication/pdf658552https://repositorio.unal.edu.co/bitstream/unal/49740/1/43861-220916-1-PB.pdf2fbcfe74ba3b23025ea227bbc8e846d9MD51THUMBNAIL43861-220916-1-PB.pdf.jpg43861-220916-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg9099https://repositorio.unal.edu.co/bitstream/unal/49740/2/43861-220916-1-PB.pdf.jpg71a5f8d97484e64f7e50c00b8347be92MD52unal/49740oai:repositorio.unal.edu.co:unal/497402023-12-11 23:07:19.426Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) |
title |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) |
spellingShingle |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) Embryo culture recalcitrance plant growth regulators tissue culture plant propagation fruit. |
title_short |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) |
title_full |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) |
title_fullStr |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) |
title_full_unstemmed |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) |
title_sort |
Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) |
dc.creator.fl_str_mv |
Criollo, Hernando Perea, Margarita Toribio, Mariano Muñoz, Johanna |
dc.contributor.author.spa.fl_str_mv |
Criollo, Hernando Perea, Margarita Toribio, Mariano Muñoz, Johanna |
dc.subject.proposal.spa.fl_str_mv |
Embryo culture recalcitrance plant growth regulators tissue culture plant propagation fruit. |
topic |
Embryo culture recalcitrance plant growth regulators tissue culture plant propagation fruit. |
description |
Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 μM of NAA were added to the medium with sucrose (50.0 and 263.1 μM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 μM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 μM of NAA + 263.1 μM of sucrose (32%). |
publishDate |
2014 |
dc.date.issued.spa.fl_str_mv |
2014-08-09 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-29T09:15:44Z |
dc.date.available.spa.fl_str_mv |
2019-06-29T09:15:44Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/49740 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/43228/ |
url |
https://repositorio.unal.edu.co/handle/unal/49740 http://bdigital.unal.edu.co/43228/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/agrocol/article/view/43861 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
dc.relation.ispartofseries.none.fl_str_mv |
Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965 |
dc.relation.references.spa.fl_str_mv |
Criollo, Hernando and Perea, Margarita and Toribio, Mariano and Muñoz, Johanna (2014) Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.). Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/49740/1/43861-220916-1-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/49740/2/43861-220916-1-PB.pdf.jpg |
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