Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)

Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant pro...

Full description

Autores:
Criollo, Hernando
Perea, Margarita
Toribio, Mariano
Muñoz, Johanna
Tipo de recurso:
Article of journal
Fecha de publicación:
2014
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/49740
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/49740
http://bdigital.unal.edu.co/43228/
Palabra clave:
Embryo culture
recalcitrance
plant growth regulators
tissue culture
plant propagation
fruit.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_408f9bd39933405c8d8e3dfae47e65fd
oai_identifier_str oai:repositorio.unal.edu.co:unal/49740
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Criollo, Hernando5fa44036-f98e-49bc-acc8-a1da9e0b752a300Perea, Margaritae13f1753-10a9-4aaa-a399-ec776c0ed241300Toribio, Mariano26d0285f-5f49-4f61-93b9-6eeccd0e1cd6300Muñoz, Johanna99d27381-d9d5-4013-80a9-5cde8c1ef8d73002019-06-29T09:15:44Z2019-06-29T09:15:44Z2014-08-09https://repositorio.unal.edu.co/handle/unal/49740http://bdigital.unal.edu.co/43228/Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 μM of NAA were added to the medium with sucrose (50.0 and 263.1 μM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 μM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 μM of NAA + 263.1 μM of sucrose (32%).application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/43861Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965Criollo, Hernando and Perea, Margarita and Toribio, Mariano and Muñoz, Johanna (2014) Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.). Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965 .Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTEmbryo culturerecalcitranceplant growth regulatorstissue cultureplant propagationfruit.ORIGINAL43861-220916-1-PB.pdfapplication/pdf658552https://repositorio.unal.edu.co/bitstream/unal/49740/1/43861-220916-1-PB.pdf2fbcfe74ba3b23025ea227bbc8e846d9MD51THUMBNAIL43861-220916-1-PB.pdf.jpg43861-220916-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg9099https://repositorio.unal.edu.co/bitstream/unal/49740/2/43861-220916-1-PB.pdf.jpg71a5f8d97484e64f7e50c00b8347be92MD52unal/49740oai:repositorio.unal.edu.co:unal/497402023-12-11 23:07:19.426Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
title Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
spellingShingle Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
Embryo culture
recalcitrance
plant growth regulators
tissue culture
plant propagation
fruit.
title_short Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
title_full Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
title_fullStr Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
title_full_unstemmed Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
title_sort Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.)
dc.creator.fl_str_mv Criollo, Hernando
Perea, Margarita
Toribio, Mariano
Muñoz, Johanna
dc.contributor.author.spa.fl_str_mv Criollo, Hernando
Perea, Margarita
Toribio, Mariano
Muñoz, Johanna
dc.subject.proposal.spa.fl_str_mv Embryo culture
recalcitrance
plant growth regulators
tissue culture
plant propagation
fruit.
topic Embryo culture
recalcitrance
plant growth regulators
tissue culture
plant propagation
fruit.
description Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 μM of NAA were added to the medium with sucrose (50.0 and 263.1 μM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 μM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 μM of NAA + 263.1 μM of sucrose (32%).
publishDate 2014
dc.date.issued.spa.fl_str_mv 2014-08-09
dc.date.accessioned.spa.fl_str_mv 2019-06-29T09:15:44Z
dc.date.available.spa.fl_str_mv 2019-06-29T09:15:44Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/49740
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/43228/
url https://repositorio.unal.edu.co/handle/unal/49740
http://bdigital.unal.edu.co/43228/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/agrocol/article/view/43861
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.ispartofseries.none.fl_str_mv Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965
dc.relation.references.spa.fl_str_mv Criollo, Hernando and Perea, Margarita and Toribio, Mariano and Muñoz, Johanna (2014) Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.). Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 Agronomía Colombiana; Vol. 32, núm. 2 (2014); 170-179 2357-3732 0120-9965 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/49740/1/43861-220916-1-PB.pdf
https://repositorio.unal.edu.co/bitstream/unal/49740/2/43861-220916-1-PB.pdf.jpg
bitstream.checksum.fl_str_mv 2fbcfe74ba3b23025ea227bbc8e846d9
71a5f8d97484e64f7e50c00b8347be92
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
_version_ 1814089599379570688