Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama

ilustraciones, diagramas, fotografías, tablas

Autores:: Molina Bejarano, Jorge Luis

Tipo de recurso:

Fecha de publicación:: 2024

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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oai_identifier_str	oai:repositorio.unal.edu.co:unal/86369
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama
dc.title.translated.eng.fl_str_mv	Characterization of exosome-like extracellular vesicles with VEGF marker in a breast cancer model
title	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama
spellingShingle	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama 570 - Biología::572 - Bioquímica 610 - Medicina y salud::616 - Enfermedades Neoplasias de la Mama Vesículas Extracelulares Exosomas Breast Neoplasms Extracellular Vesicles Exosomes Cromatografía de exclusión por tamaño Hipoxia Ultracentrifugación diferencial Angiogénesis Factor de crecimiento vascular endotelial Size exclusion chromatography Differential ultracentrifugation Hypoxia Angiogenesis Vascular endotelial growth factor
title_short	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama
title_full	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama
title_fullStr	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama
title_full_unstemmed	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama
title_sort	Caracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mama
dc.creator.fl_str_mv	Molina Bejarano, Jorge Luis
dc.contributor.advisor.none.fl_str_mv	Umaña Pérez, Yadi Adriana
dc.contributor.author.none.fl_str_mv	Molina Bejarano, Jorge Luis
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Hormonas
dc.subject.ddc.spa.fl_str_mv	570 - Biología::572 - Bioquímica 610 - Medicina y salud::616 - Enfermedades
topic	570 - Biología::572 - Bioquímica 610 - Medicina y salud::616 - Enfermedades Neoplasias de la Mama Vesículas Extracelulares Exosomas Breast Neoplasms Extracellular Vesicles Exosomes Cromatografía de exclusión por tamaño Hipoxia Ultracentrifugación diferencial Angiogénesis Factor de crecimiento vascular endotelial Size exclusion chromatography Differential ultracentrifugation Hypoxia Angiogenesis Vascular endotelial growth factor
dc.subject.decs.spa.fl_str_mv	Neoplasias de la Mama Vesículas Extracelulares Exosomas
dc.subject.decs.eng.fl_str_mv	Breast Neoplasms Extracellular Vesicles Exosomes
dc.subject.proposal.spa.fl_str_mv	Cromatografía de exclusión por tamaño Hipoxia Ultracentrifugación diferencial Angiogénesis Factor de crecimiento vascular endotelial
dc.subject.proposal.eng.fl_str_mv	Size exclusion chromatography Differential ultracentrifugation Hypoxia Angiogenesis Vascular endotelial growth factor
description	ilustraciones, diagramas, fotografías, tablas
publishDate	2024
dc.date.accessioned.none.fl_str_mv	2024-07-03T14:13:06Z
dc.date.available.none.fl_str_mv	2024-07-03T14:13:06Z
dc.date.issued.none.fl_str_mv	2024
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/86369
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/86369 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
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dc.format.extent.spa.fl_str_mv	xvii, 89 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Maestría en Ciencias - Bioquímica
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Umaña Pérez, Yadi Adrianad652013090d46425f836051252c18e62Molina Bejarano, Jorge Luis7719e6757eb07e28d65e28add9e09d1eGrupo de Investigación en Hormonas2024-07-03T14:13:06Z2024-07-03T14:13:06Z2024https://repositorio.unal.edu.co/handle/unal/86369Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografías, tablasLos exosomas, un tipo de vesícula extracelular, emergen en condiciones fisiológicas y patológicas como en el cáncer de mama, desempeñando un papel clave en la comunicación intercelular dentro y fuera del microambiente tumoral. Para comprender su función es esencial realizar la caracterización física y molecular de su composición, así como establecer sus interacciones en contextos biológicos. En este trabajo, se obtuvieron exosomas provenientes del cultivo in vitro de la línea celular de cáncer de mama MCF7. En condiciones de normoxia, se evaluaron dos métodos comerciales, uno basado en cromatografía de exclusión por tamaño y otro en filtración dirigida. Los exosomas purificados se compararon con los obtenidos mediante el método tradicional de ultracentrifugación diferencial, y la cromatografía de exclusión por tamaño se seleccionó como el método con mayor rendimiento, economía y accesibilidad para la purificación de exosomas. Posteriormente, se obtuvieron exosomas de la misma línea celular en condiciones de hipoxia mimética inducida por cloruro de cobalto, observando una disminución tanto en la producción como en el tamaño de los exosomas comparados a la condición de normoxia, sumado a una mayor producción de VEGF, aunque no estuvo asociado directamente a los exosomas. Finalmente, se observó que los exosomas producidos en hipoxia mimética favorecen la migración en células MCF7 y promueven características específicas en la formación de tubos en la angiogénesis inducida sobre células HUVEC (Texto tomado de la fuente).Exosomes, a type of extracellular vesicle, arise in physiological and pathological conditions, such as breast cancer. They play a crucial role in intercellular communication within and outside the tumor microenvironment. To comprehend their function, it is essential to conduct a physical and molecular characterization of their composition and establish their interactions in biological contexts. In this work, exosomes were obtained from in vitro culture of the MCF7 breast cancer cell line. Under normoxic conditions, two commercial methods were evaluated, one based on size exclusion chromatography and the other on targeted filtration. Purified exosomes were compared with those obtained through the traditional method of differential ultracentrifugation, and size exclusion chromatography was chosen as the most economical and accessible method for exosome purification. Subsequently, exosomes were produced by the same cell line under conditions of mimetic hypoxia induced by cobalt chloride, showing a decrease in the production and size of exosomes compared to normoxia. Additionally, it was identified that VEGF was produced in higher concentrations under hypoxic conditions, although it was not associated with exosomes. Finally, it was observed that exosomes produced under mimetic hypoxia promote migration in MCF7 cells and promote specific characteristics in tube formation in angiogenesis induced in HUVEC cells.MaestríaMagíster en Ciencias - BioquímicaFactores de crecimiento, diferenciación y cáncerxvii, 89 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - Bioquímica610 - Medicina y salud::616 - EnfermedadesNeoplasias de la MamaVesículas ExtracelularesExosomasBreast NeoplasmsExtracellular VesiclesExosomesCromatografía de exclusión por tamañoHipoxiaUltracentrifugación diferencialAngiogénesisFactor de crecimiento vascular endotelialSize exclusion chromatographyDifferential ultracentrifugationHypoxiaAngiogenesisVascular endotelial growth factorCaracterización de vesículas extracelulares tipo exosomas con marcador VEGF en un modelo de cáncer de mamaCharacterization of exosome-like extracellular vesicles with VEGF marker in a breast cancer modelTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMCancer IAfRo. 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Traffic. 2021;22(7):204-20EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86369/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1018453231.2024.pdf1018453231.2024.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf6526869https://repositorio.unal.edu.co/bitstream/unal/86369/2/1018453231.2024.pdf2e68c3fb5b5fded32e9a3f1879ec13ddMD52THUMBNAIL1018453231.2024.pdf.jpg1018453231.2024.pdf.jpgGenerated Thumbnailimage/jpeg5191https://repositorio.unal.edu.co/bitstream/unal/86369/3/1018453231.2024.pdf.jpge023c54663d99ee218452a9f23aaf3a5MD53unal/86369oai:repositorio.unal.edu.co:unal/863692024-08-25 23:11:53.662Repositorio Institucional Universidad Nacional de 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