Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata
ilustraciones, graficas
- Autores:
-
Pulido Teuta, Juanita
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/83893
- Palabra clave:
- 540 - Química y ciencias afines::547 - Química orgánica
Enfermedad de Alzheimer
Alzheimer Disease
Alzheimer
Modelamiento molecular
Nectandra
Lauraceae
Box-Bhencken
Validación
Docking molecular
Validation
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata |
| dc.title.translated.eng.fl_str_mv |
Use of design of experiments for the optimization of the extraction of phenolic compounds in an active extract of Nectandra Reticulata |
| title |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata |
| spellingShingle |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata 540 - Química y ciencias afines::547 - Química orgánica Enfermedad de Alzheimer Alzheimer Disease Alzheimer Modelamiento molecular Nectandra Lauraceae Box-Bhencken Validación Docking molecular Validation |
| title_short |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata |
| title_full |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata |
| title_fullStr |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata |
| title_full_unstemmed |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata |
| title_sort |
Uso de diseño de experimentos para la optimización de la extracción de compuestos fenólicos en un extracto activo de Nectandra Reticulata |
| dc.creator.fl_str_mv |
Pulido Teuta, Juanita |
| dc.contributor.advisor.none.fl_str_mv |
Ávila Murillo, Mónica Constanza Narváez Cuenca, Carlos Eduardo |
| dc.contributor.author.none.fl_str_mv |
Pulido Teuta, Juanita |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Química de Productos Naturales Vegetales Bioactivos (Quipronab) |
| dc.subject.ddc.spa.fl_str_mv |
540 - Química y ciencias afines::547 - Química orgánica |
| topic |
540 - Química y ciencias afines::547 - Química orgánica Enfermedad de Alzheimer Alzheimer Disease Alzheimer Modelamiento molecular Nectandra Lauraceae Box-Bhencken Validación Docking molecular Validation |
| dc.subject.decs.spa.fl_str_mv |
Enfermedad de Alzheimer |
| dc.subject.decs.eng.fl_str_mv |
Alzheimer Disease |
| dc.subject.proposal.spa.fl_str_mv |
Alzheimer Modelamiento molecular Nectandra |
| dc.subject.proposal.eng.fl_str_mv |
Lauraceae Box-Bhencken Validación Docking molecular Validation |
| description |
ilustraciones, graficas |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2023-05-29T16:28:25Z |
| dc.date.available.none.fl_str_mv |
2023-05-29T16:28:25Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/83893 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/83893 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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R., Cecchi, T., Swain, S., Patro, C. S., & Panda, J. (2018). An overview of experimental designs in HPLC method development and validation. Journal of Pharmaceutical and Biomedical Analysis, 147, 590-611. https://doi.org/10.1016/j.jpba.2017.05.006 Sanabria-Castro, A., Alvarado-Echeverría, I., & Monge-Bonilla, C. (2017). Molecular Pathogenesis of Alzheimer’s Disease: An Update. Annals of Neurosciences, 24(1), 46-54. https://doi.org/10.1159/000464422 Sang, Z., Wang, K., Dong, J., & Tang, L. (2022). Alzheimer’s disease: Updated multi-targets therapeutics are in clinical and in progress. European Journal of Medicinal Chemistry, 238, 114464. https://doi.org/10.1016/j.ejmech.2022.114464 Serrano, M. P. (2010). Mecanismos bioquímicos de la Enfermedad de Alzheimer. 20. Sever, R., & Glass, C. K. (2013). Signaling by Nuclear Receptors. Cold Spring Harbor Perspectives in Biology, 5(3), a016709-a016709. https://doi.org/10.1101/cshperspect.a016709 Sodhi, R. K., & Singh, N. (2013). Liver X receptors: Emerging therapeutic targets for Alzheimer’s disease. Pharmacological Research, 72, 45-51. https://doi.org/10.1016/j.phrs.2013.03.008 Stipičević, S., Fingler, S., Zupančič-Kralj, L., & Drevenkar, V. (2003). Comparison of gas and high performance liquid chromatography with selective detection for determination of triazine herbicides and their degradation products extracted ultrasonically from soil. Journal of Separation Science, 26(14), 1237-1246. https://doi.org/10.1002/jssc.200301420 Tamayo, A. E. I., Pérez, C. H., & Tejeda, J. J. G. (2020). Tratamientos paliativos en la enfermedad de Alzheimer. 16 de abril, 59(275), 1-6. Taverniers, I., De Loose, M., & Van Bockstaele, E. (2004). Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance. TrAC Trends in Analytical Chemistry, 23(8), 535-552. https://doi.org/10.1016/j.trac.2004.04.001 Tolosa, T., Rogez, H., Silva, E., & Souza, J. (2018). Optimization of Acid Hydrolysis of Myricetin-3-O-rhamnoside Using Response Surface Methodology. Journal of the Brazilian Chemical Society. https://doi.org/10.21577/0103-5053.20180125 Tundis, R., Loizzo, M. R., Nabavi, S. M., Orhan, I. E., Skalicka-Woźniak, K., D’Onofrio, G., & Aiello, F. (2018). Chapter 3—Natural Compounds and Their Derivatives as Multifunctional Agents for the Treatment of Alzheimer Disease. En G. Brahmachari (Ed.), Discovery and Development of Neuroprotective Agents from Natural Products (pp. 63-102). Elsevier. https://doi.org/10.1016/B978-0-12-809593-5.00003-3 Valencia Rincón, E. (2018). Generación de un modelo in vitro para evaluar la actividad agonista de extractos naturales, obtenidos de plantas de las familias de Lauráceas y Miristicáceas, sobre los receptores X del hígado (LXRs). https://repositorio.unal.edu.co/handle/unal/63367 Veer, B., Geetanjali, & Singh, R. (2020). Chapter 6—Natural products as anti-Alzheimer’s drugs. 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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ávila Murillo, Mónica Constanzac7e9747034b686246f89d8af2147f989Narváez Cuenca, Carlos Eduardo29b6cc08a0076e18f5d99fa13748372fPulido Teuta, Juanita27c04aff983dd400d47f1ecee6983b8eGrupo de Investigación en Química de Productos Naturales Vegetales Bioactivos (Quipronab)2023-05-29T16:28:25Z2023-05-29T16:28:25Z2022https://repositorio.unal.edu.co/handle/unal/83893Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, graficasLa actividad agonista en LXR modula la producción de ApoE y ABCA1, disminuyendo la placa amiloide en modelos murinos, convirtiéndose así una alternativa terapéutica del Alzheimer. Estudios previos muestran que Nectandra reticulata es agonista de LXR, por lo que es de nuestro interés identificar la composición del extracto, relacionarlo con su actividad y optimizar la extracción de este. Es así cómo se analizó el extracto activo de N. reticulata, mediante RP-UHPLC-DAD y UHPLC-ESI-HRMS. Los resultados del análisis por EM evidenciaron que la mayoría de los compuestos presentes corresponden a flavonoides glicosilados. Para confirmar la identidad de las agliconas, se realizó una hidrólisis ácida, mostrando que el compuesto principal con un área cromatográfica de 84,5% a 355 nm corresponde a la quercitrina; el segundo compuesto en abundancia del 8,0% del área cromatográfica se identificó como afzelina. En menor proporción (6,9%) se determinó 3 o 7-(6''-p-cumaroilglucósido) kaempferol. Después de la identificación se realizaron estudios in silico e in vitro, permitiendo seleccionar a la quercitrina cómo marcador, ya que este metabolito presenta una actividad superior a la de los otros compuestos identificados e incluso de su respectiva aglicona. Finalmente se realizó la validación y optimización de la extracción de este metabolito, se encontró que al hacer la extracción asistida por ultrasonido la cantidad de quercitrina en el extracto y el rendimiento del proceso se ven influenciados principalmente por la proporción etanol:agua y la temperatura del sistema. La mejores condiciones de extracción encontradas fueron 60% de etanol, 50 °C y 40 mL/g (solvente:material vegetal). (Texto tomado de la fuente)The agonistic activity of LXR modulates the production of ApoE and ABCA1, reducing amyloid plaque in murine models, that means a therapeutic alternative for Alzheimer's. Previous studies show that Nectandra reticulata is an LXR agonist, it is our interest to identify the composition of the extract, relate it to its activity and optimize its extraction. The active extract of N. reticulata was analyzed by RP-UHPLC-DAD and UHPLC-ESI-HRMS. The mass analysis showed that most of the compounds present correspond to glycosylated flavonoids. To confirm the identity of the aglycones, acid hydrolysis was performed. There it was shown that the main compound with a chromatographic area of 84.5% at 355 nm corresponds to quercitrin; the second compound in abundance of 8.0% of the chromatographic area was identified as afzeline. In a smaller proportion (chromatographic area 6.9%) 3 or 7-(6''-p-coumaroylglucoside) kaempferol were found. After the identification, in silico and in vitro studies of the molecules were carried out, these studies allowed quercitrin to be selected as a marker, since this metabolite has a higher activity than the other identified compounds and even of its respective aglycone. Finally, the validation and optimization of the extraction of this metabolite was carried out, it was found that when performing the extraction assisted by ultrasound, the amount of quercitrin in the extract and the performance of the process are mainly influenced by the proportion of ethanol:water and temperature of the system. 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Journal of Experimental Botany, 63(7), 2465-2478. https://doi.org/10.1093/jxb/err416EstudiantesInvestigadoresPúblico generalORIGINAL1015474719_2023.pdf1015474719_2023.pdfTesis de Maestría en Ciencias- Químicaapplication/pdf1706268https://repositorio.unal.edu.co/bitstream/unal/83893/2/1015474719_2023.pdfa0d03f89546ff85b795e462ccc57f4c1MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83893/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51THUMBNAIL1015474719_2023.pdf.jpg1015474719_2023.pdf.jpgGenerated Thumbnailimage/jpeg5710https://repositorio.unal.edu.co/bitstream/unal/83893/3/1015474719_2023.pdf.jpg7fd0887d1a8bf7b7fcca7ad29f0f76e6MD53unal/83893oai:repositorio.unal.edu.co:unal/838932024-08-07 23:10:50.473Repositorio Institucional Universidad Nacional de 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