Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L)
Since the 19th century, coffee has been the main agricultural export product in Colombia, guaranteeing an important contribution to the country's economic development. The disease called "The leak", caused by the fungus O. flavida (anamorphic phase of M. citricolor), occurs very frequ...
- Autores:
-
Arciniegas Grijalba, Paola Andrea
- Tipo de recurso:
- Informe
- Fecha de publicación:
- 2021
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/78811
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/78811
- Palabra clave:
- 630 - Agricultura y tecnologías relacionadas
Mycena citricolor
Morphology
Pathogenicity
Antifungical
Mycena citricolor
Morfología
Patogenicidad
Antifúngica
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) |
| title |
Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) |
| spellingShingle |
Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) 630 - Agricultura y tecnologías relacionadas Mycena citricolor Morphology Pathogenicity Antifungical Mycena citricolor Morfología Patogenicidad Antifúngica |
| title_short |
Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) |
| title_full |
Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) |
| title_fullStr |
Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) |
| title_full_unstemmed |
Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) |
| title_sort |
Uso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L) |
| dc.creator.fl_str_mv |
Arciniegas Grijalba, Paola Andrea |
| dc.contributor.advisor.spa.fl_str_mv |
Muñoz Florez, Jaime Eduardo Guerra Sierra, Beatriz Elena Mosquera Sánchez, Lyda Patricia |
| dc.contributor.author.spa.fl_str_mv |
Arciniegas Grijalba, Paola Andrea |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Diversidad Biológica |
| dc.subject.ddc.spa.fl_str_mv |
630 - Agricultura y tecnologías relacionadas |
| topic |
630 - Agricultura y tecnologías relacionadas Mycena citricolor Morphology Pathogenicity Antifungical Mycena citricolor Morfología Patogenicidad Antifúngica |
| dc.subject.proposal.eng.fl_str_mv |
Mycena citricolor Morphology Pathogenicity Antifungical |
| dc.subject.proposal.spa.fl_str_mv |
Mycena citricolor Morfología Patogenicidad Antifúngica |
| description |
Since the 19th century, coffee has been the main agricultural export product in Colombia, guaranteeing an important contribution to the country's economic development. The disease called "The leak", caused by the fungus O. flavida (anamorphic phase of M. citricolor), occurs very frequently in coffee plants, whose main damage is defoliation. In the present study the in vitro characterization of the morphological and molecular variation of 16 and seven isolates of O. flavida, respectively; from four coffee producing municipalities in Cauca. Likewise, the in vitro pathogenicity of seven isolates in healthy coffee leaves of the Castillo® El Tambo Variety was determined, and finally the antifungal effect of ZnO-NPs was evaluated, specifically on two O. flavida isolates that presented a high level of pathogenicity. The results showed that the isolates were grouped into 4 morphotypes, according to the geographical area and the similarity of their morphological and cultural characteristics; accordingly, significant differences were found for the mycelial growth rate index (IVCM) except between the El Tambo and Bolívar isolates and (P = 0.351), there was no significant difference for the percentage of gems among the isolates evaluated by municipality (P=0.355), the vegetative and reproductive structures presented typical characteristics of the fungus, in addition significant differences were observed for the diameter of the head for both La Sierra and Caloto (P = 0.026), isolates, there were no differences in the length of the total pedicel of the gem for those isolates (P = 0.835) and there were differences in the total length of the gem for El Tambo and Caloto (P = 0.001) isolates. The high percentage of identity and the general BLASTn results indicated that the isolates would correspond to O. flavida. Regarding the evaluation of pathogenicity, the morphotypes of El Tambo and La Sierra had the highest levels of pathogenicity. The NPs based on ZnO showed an antifungal activity on the isolates that presented the highest levels of pathogenicity, the most efficient treatment was with 12 mmol.L |
| publishDate |
2021 |
| dc.date.accessioned.spa.fl_str_mv |
2021-01-19T04:56:03Z |
| dc.date.available.spa.fl_str_mv |
2021-01-19T04:56:03Z |
| dc.date.issued.spa.fl_str_mv |
2021-01-18 |
| dc.type.spa.fl_str_mv |
Documento de trabajo |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_8042 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/workingPaper |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_93fc |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/WP |
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http://purl.org/coar/resource_type/c_93fc |
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https://repositorio.unal.edu.co/handle/unal/78811 |
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https://repositorio.unal.edu.co/handle/unal/78811 |
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spa |
| language |
spa |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de ColombiaAcceso abiertohttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Muñoz Florez, Jaime Eduardo0d0f912e-5d4a-4351-b5b1-348a4ab59ff4-1Guerra Sierra, Beatriz Elena510da4a8-a949-4832-8892-9e3f5ac96962-1Mosquera Sánchez, Lyda Patriciaef3eb8a6-a62c-477a-bc56-33f0e00291e0-1Arciniegas Grijalba, Paola Andreab528a58b-ff29-498a-b11e-f89a78c573d4Grupo de Investigación en Diversidad Biológica2021-01-19T04:56:03Z2021-01-19T04:56:03Z2021-01-18https://repositorio.unal.edu.co/handle/unal/78811Since the 19th century, coffee has been the main agricultural export product in Colombia, guaranteeing an important contribution to the country's economic development. The disease called "The leak", caused by the fungus O. flavida (anamorphic phase of M. citricolor), occurs very frequently in coffee plants, whose main damage is defoliation. In the present study the in vitro characterization of the morphological and molecular variation of 16 and seven isolates of O. flavida, respectively; from four coffee producing municipalities in Cauca. Likewise, the in vitro pathogenicity of seven isolates in healthy coffee leaves of the Castillo® El Tambo Variety was determined, and finally the antifungal effect of ZnO-NPs was evaluated, specifically on two O. flavida isolates that presented a high level of pathogenicity. The results showed that the isolates were grouped into 4 morphotypes, according to the geographical area and the similarity of their morphological and cultural characteristics; accordingly, significant differences were found for the mycelial growth rate index (IVCM) except between the El Tambo and Bolívar isolates and (P = 0.351), there was no significant difference for the percentage of gems among the isolates evaluated by municipality (P=0.355), the vegetative and reproductive structures presented typical characteristics of the fungus, in addition significant differences were observed for the diameter of the head for both La Sierra and Caloto (P = 0.026), isolates, there were no differences in the length of the total pedicel of the gem for those isolates (P = 0.835) and there were differences in the total length of the gem for El Tambo and Caloto (P = 0.001) isolates. The high percentage of identity and the general BLASTn results indicated that the isolates would correspond to O. flavida. Regarding the evaluation of pathogenicity, the morphotypes of El Tambo and La Sierra had the highest levels of pathogenicity. The NPs based on ZnO showed an antifungal activity on the isolates that presented the highest levels of pathogenicity, the most efficient treatment was with 12 mmol.LDesde el siglo XIX el café ha sido el principal producto agrícola de exportación en Colombia, garantizando un importante aporte al desarrollo económico del país. La enfermedad denominada “La gotera”, causada por el hongo Omphalia flavida (fase anamorfa de Mycena citricolor), se presenta muy frecuentemente en las plantas de café, cuyo daño principal es la defoliación. En el presente estudio se realizó la caracterización in vitro de la variación morfológica y molecular de 16 y 7 aislamientos de O. flavida, respectivamente; provenientes de cuatro municipios productores de café en el Cauca. Así mismo, se determinó la patogenicidad in vitro de 7 aislamientos en hojas sanas de café de la variedad Castillo® El Tambo, para evaluar el efecto antifúngico de las ZnO- NPs, específicamente sobre 2 aislamientos de O. flavida que presentaron un alto nivel de patogenicidad. Los resultados mostraron que los aislamientos fueron agrupados en 4 morfotipos, de acuerdo al área geográfica y la similitud de sus características morfológicas y culturales; de acuerdo a ello se encontraron diferencias significativas para el índice de velocidad de crecimiento micelial (IVCM) excepto entre los aislamientos de El Tambo y Bolívar, no hubo diferencia significativa para el porcentaje de gemas entre los aislamientos evaluados por municipio, las estructuras vegetativas y reproductivas presentaron características propias reportadas en la literatura, se observaron diferencias significativas para el diámetro de la cabezuela de los aislamientos de La Sierra y Caloto, no hubo diferencias en la longitud del pedicelo y si hubo diferencias en la longitud total de la gema para los de El Tambo y Caloto. El alto porcentaje de identidad y los resultados generales del BLASTn indicaron que los aislamientos corresponden a O. flavida. En cuanto a la evaluación de la patogenicidad, los morfotipos de El Tambo y La Sierra presentaron los niveles más altos de patogenicidad. Las nanopartículas con base en ZnO mostraron una actividad antifúngica sobre los aislamientos que presentaron los niveles más altos de patogenicidad, el tratamiento más eficiente fue con 12 mmol. L -1.Maestría84application/pdfspa630 - Agricultura y tecnologías relacionadasMycena citricolorMorphologyPathogenicityAntifungicalMycena citricolorMorfologíaPatogenicidadAntifúngicaUso de nanopartículas de óxido de cinc como control de Omphalia flavida, agente causal de “la gotera”; enfermedad del cafeto (Coffea arábica L)Documento de trabajoinfo:eu-repo/semantics/workingPaperinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_93fchttp://purl.org/coar/resource_type/c_8042http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/WPPalmira - Ciencias Agropecuarias - Maestría en Ciencias BiológicasMaestría en Ciencias BiológicasUniversidad Nacional de Colombia - Sede PalmiraAbd-Elsalam, K. A., & Alghuthaymi, M. A. (2015). Nanobiofungicides: are they the next-generation of fungicides. J Nanotech Mater Sci, 2, 1–3. https://doi.org/10.15436/2377-1372.15.0Anderson, I. C., & Cairney, J. W. G. (2004). 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