Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster

ilustraciones, diagramas, tablas

Autores:
Rodríguez Pineda, Mario Andrés
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2020
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
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oai:repositorio.unal.edu.co:unal/80816
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/80816
https:/repositorio.una.edu.co
Palabra clave:
660 - Ingeniería química
Dinámica molecular
Molecular dynamics
Intercambiadores Na+ /Ca2+
Dinámica de Proteínas
Espectroscopia de Resonancia Magnética Nuclear
Simulaciones de Dinámica Molecular
Na+ /Ca2+ Exchangers
Molecular Dynamics Simulations
Nuclear Magnetic Resonance Spectroscopy
Protein Dynamics
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openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_38ea67cfde1df489f3fbf79f79315616
oai_identifier_str oai:repositorio.unal.edu.co:unal/80816
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
dc.title.translated.eng.fl_str_mv NMR studies of the calcium sensing domain of the Na+ /Ca2+ exchanger of Drosophila melanogaster
title Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
spellingShingle Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
660 - Ingeniería química
Dinámica molecular
Molecular dynamics
Intercambiadores Na+ /Ca2+
Dinámica de Proteínas
Espectroscopia de Resonancia Magnética Nuclear
Simulaciones de Dinámica Molecular
Na+ /Ca2+ Exchangers
Molecular Dynamics Simulations
Nuclear Magnetic Resonance Spectroscopy
Protein Dynamics
title_short Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
title_full Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
title_fullStr Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
title_full_unstemmed Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
title_sort Estudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogaster
dc.creator.fl_str_mv Rodríguez Pineda, Mario Andrés
dc.contributor.advisor.none.fl_str_mv Kopke Salinas, Roberto
Espejo Benavides, Blanca Fabiola
dc.contributor.author.none.fl_str_mv Rodríguez Pineda, Mario Andrés
dc.contributor.researchgroup.spa.fl_str_mv Metabolismo de Calcio y Mecanismos de competición entre microorganismos
dc.subject.ddc.spa.fl_str_mv 660 - Ingeniería química
topic 660 - Ingeniería química
Dinámica molecular
Molecular dynamics
Intercambiadores Na+ /Ca2+
Dinámica de Proteínas
Espectroscopia de Resonancia Magnética Nuclear
Simulaciones de Dinámica Molecular
Na+ /Ca2+ Exchangers
Molecular Dynamics Simulations
Nuclear Magnetic Resonance Spectroscopy
Protein Dynamics
dc.subject.lemb.none.fl_str_mv Dinámica molecular
Molecular dynamics
dc.subject.proposal.spa.fl_str_mv Intercambiadores Na+ /Ca2+
Dinámica de Proteínas
Espectroscopia de Resonancia Magnética Nuclear
Simulaciones de Dinámica Molecular
dc.subject.proposal.eng.fl_str_mv Na+ /Ca2+ Exchangers
Molecular Dynamics Simulations
Nuclear Magnetic Resonance Spectroscopy
Protein Dynamics
description ilustraciones, diagramas, tablas
publishDate 2020
dc.date.issued.none.fl_str_mv 2020
dc.date.accessioned.none.fl_str_mv 2022-01-31T18:29:58Z
dc.date.available.none.fl_str_mv 2022-01-31T18:29:58Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/80816
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https:/repositorio.una.edu.co
url https://repositorio.unal.edu.co/handle/unal/80816
https:/repositorio.una.edu.co
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
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institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Kopke Salinas, Roberto066b2098fc4bd2e5758060c0d357cbe5600Espejo Benavides, Blanca Fabiolacdd4a63c80876abe8cd877ea46c8dc34600Rodríguez Pineda, Mario Andrés1a779c1803083961771dc8108a96f422Metabolismo de Calcio y Mecanismos de competición entre microorganismos2022-01-31T18:29:58Z2022-01-31T18:29:58Z2020https://repositorio.unal.edu.co/handle/unal/80816Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps:/repositorio.una.edu.coilustraciones, diagramas, tablasLos intercambiadores de sodio-calcio (Na+ /Ca2+ exchangers, NCX) componen una amplia familia de intercambiadores presentes en casi todos los organismos, estando involucrados en la homeostasis celular del Ca2+. Una de estas proteínas de intercambio iónico que ha presentado gran interés para los científicos es el intercambiador (CALX) de Drosophila melanogaster (mosca de la fruta), debido a que la presencia de Ca2+ intracelular inhibe el intercambio iónico en CALX, mientras que en mamíferos (especialmente en NCX1) el incremento de [Ca2+]i activa el intercambiador. A demás de la diferencia con NCX, CALX presenta dos isoformas gracias al empalme (splicing) alternativo llamadas CALX-1.1 y CALX-1.2. Estas isoformas se diferencian por la mutación de 5 aminoácidos en el loop-FG de CBD2 y, porque CALX-1.1 es altamente inhibida por la presencia de Ca2+ mientras que la inhibición en CALX-1.2 es mucho menor, al punto que algunos autores llegan a establecer que es insensible al Ca2+i. Para comprender estas diferencias entre las dos isoformas de CALX, en este trabajo se llevaron a cabo estudios de dinámica molecular tanto del dominio CBD2 como del conjunto de CBD1 y CBD2 (denominado CBD12), encontrando diferencias dinámicas en CBD2, principalmente en la región del loop-FG. Según lo observado la hélice 1 en el loop-FG en CBD2 puede interactuar con la R584 en el loop-CD del mismo dominio en la isoforma 1.2 de mejor manera que en la isoforma 1.1, especialmente en la forma coordinada a Ca2+, lo que permite que CBD12 posea una orientación más compacta lo que hace que la isoforma 1.2 sea menos sensible al Ca2+ que la isoforma 1.1 (Texto tomado de la fuente)Sodium-calcium exchangers (Na+ /Ca2+ exchangers, NCX) are a wide family of exchangers present in almost all organisms, being involved in Ca2+ cellular homeostasis. One of these ion exchange proteins that has been of great interest to scientists is the Drosophila melanogaster (fruit fly) exchanger (CALX), because the presence of intracellular Ca2+ inhibits ion exchange in CALX, whereas in mammals (especially in NCX1) the increase of [Ca2+]i activates the exchanger. In addition to the difference with NCX, CALX presents two isoforms thanks to the alternative splicing called CALX-1.1 and CALX-1.2. These isoforms are differentiated by the 5 amino acid mutation in the CBD2 loop-FG and, because CALX-1.1 is highly inhibited by the presence of Ca2+ while the inhibition in CALX-1.2 is much lower, to the point that some authors reach establish that it is insensitive to Ca2+i. To understand these differences between the two CALX isoforms, in this work molecular dynamics studies of both the CBD2 domain and the set of CBD1 and CBD2 (called CBD12) were carried out, finding dynamic differences in CBD2, mainly in the FG-loop region. As observed helix 1 in FG-loop in CBD2 can interact with R584 in CD-loop of the same domain in 1.2 isoform better than in 1.1 isoform, especially in the Ca2+ binding form, allowing CBD12 has a more compact orientation making the 1.2 isoform less sensitive to Ca2+i than the 1.1 isoformCOLCIENCIASDoctoradoDoctor en BiotecnologíaConvocatoria No. 647 de 2014Biología estructural y bioquímicaÁrea Curricular de Bioctecnologíaxx, 123 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín660 - Ingeniería químicaDinámica molecularMolecular dynamicsIntercambiadores Na+ /Ca2+Dinámica de ProteínasEspectroscopia de Resonancia Magnética NuclearSimulaciones de Dinámica MolecularNa+ /Ca2+ ExchangersMolecular Dynamics SimulationsNuclear Magnetic Resonance SpectroscopyProtein DynamicsEstudios de RMN del dominio sensor de calcio del intercambiador de Na+ /Ca2+ de Drosophila melanogasterNMR studies of the calcium sensing domain of the Na+ /Ca2+ exchanger of Drosophila melanogasterTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAbiko, L. 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Methods in Enzymology, 185(C), 60–89. https://doi.org/10.1016/0076-6879(90)85008-C.FAPESPInvestigadoresORIGINAL80208848.2020.pdf80208848.2020.pdfTesis de Doctorado en Biotecnologíaapplication/pdf7570073https://repositorio.unal.edu.co/bitstream/unal/80816/1/80208848.2020.pdfc2ca0b334681f1635327630eab38f385MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/80816/2/license.txt8153f7789df02f0a4c9e079953658ab2MD52THUMBNAIL80208848.2020.pdf.jpg80208848.2020.pdf.jpgGenerated Thumbnailimage/jpeg4847https://repositorio.unal.edu.co/bitstream/unal/80816/3/80208848.2020.pdf.jpgbeb8599ccca3449625f34294162c399fMD53unal/80816oai:repositorio.unal.edu.co:unal/808162023-07-31 23:04:28.295Repositorio Institucional Universidad Nacional de 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EVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLiAqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4gCgpBbCBoYWNlciBjbGljIGVuIGVsIHNpZ3VpZW50ZSBib3TDs24sIHVzdGVkIGluZGljYSBxdWUgZXN0w6EgZGUgYWN1ZXJkbyBjb24gZXN0b3MgdMOpcm1pbm9zLiBTaSB0aWVuZSBhbGd1bmEgZHVkYSBzb2JyZSBsYSBsaWNlbmNpYSwgcG9yIGZhdm9yLCBjb250YWN0ZSBjb24gZWwgYWRtaW5pc3RyYWRvciBkZWwgc2lzdGVtYS4KClVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIC0gw5psdGltYSBtb2RpZmljYWNpw7NuIDE5LzEwLzIwMjEK

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