Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca

Ilustraciones, tablas

Autores:: Vélez Martínez, Glever Alexander

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca
dc.title.translated.eng.fl_str_mv	Metabarcoding of soil microbial communities in forest and páramo ecosystems in Valle del Cauca.
title	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca
spellingShingle	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca 570 - Biología::577 - Ecología RNA sequence Secuencia de ARN Next Generation Sequencing Microbial Communities Metagenomics Colombian terrestrial ecosystems Meta-taxonomy Microbial ecology Altitudinal gradient Ecosistemas terrestres colombianos Meta-taxonomía Ecología microbiana Gradiente altitudinal Ecosistemas terrestres Terrestrial ecosystems
title_short	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca
title_full	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca
title_fullStr	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca
title_full_unstemmed	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca
title_sort	Metabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del Cauca
dc.creator.fl_str_mv	Vélez Martínez, Glever Alexander
dc.contributor.advisor.none.fl_str_mv	Muñoz Flórez, Jaime Eduardo Rugeles Silva, Paula Andrea
dc.contributor.author.none.fl_str_mv	Vélez Martínez, Glever Alexander
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Diversidad Biológica
dc.contributor.orcid.spa.fl_str_mv	Velez Martinez, Glever Alexander [0009-0002-9029-0154] Muñoz Flórez, Jaime Eduardo [0000-0002-8237-0499] Rugeles Silva, Paula Andrea [0000-0002-7419-6638]
dc.subject.ddc.spa.fl_str_mv	570 - Biología::577 - Ecología
topic	570 - Biología::577 - Ecología RNA sequence Secuencia de ARN Next Generation Sequencing Microbial Communities Metagenomics Colombian terrestrial ecosystems Meta-taxonomy Microbial ecology Altitudinal gradient Ecosistemas terrestres colombianos Meta-taxonomía Ecología microbiana Gradiente altitudinal Ecosistemas terrestres Terrestrial ecosystems
dc.subject.agrovoc.none.fl_str_mv	RNA sequence Secuencia de ARN Next Generation Sequencing Microbial Communities Metagenomics
dc.subject.proposal.eng.fl_str_mv	Colombian terrestrial ecosystems Meta-taxonomy Microbial ecology Altitudinal gradient
dc.subject.proposal.spa.fl_str_mv	Ecosistemas terrestres colombianos Meta-taxonomía Ecología microbiana Gradiente altitudinal
dc.subject.unesco.none.fl_str_mv	Ecosistemas terrestres Terrestrial ecosystems
description	Ilustraciones, tablas
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-06-05T16:51:04Z
dc.date.available.none.fl_str_mv	2023-06-05T16:51:04Z
dc.date.issued.none.fl_str_mv	2023-04-20
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/83966
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/83966 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.references.spa.fl_str_mv	Ashraf, M., Hussain, M., Ahmad, M. S. A., Al-Qurainy, F., & Hameed, M. (2012). Strategies for conservation of endangered ecosystems. Pakistan Journal of Botany, 44 (SPL. ISS. 2). Bayranvand, M., Akbarinia, M., Salehi Jouzani, G., Gharechahi, J., Kooch, Y., & Baldrian, P. (2021). Composition of soil bacterial and fungal communities in relation to vegetation composition and soil characteristics along an altitudinal gradient. FEMS Microbiology Ecology, 97(1). https://doi.org/10.1093/femsec/fiaa201 Bellard, C., Bertelsmeier, C., Leadley, P., Thuiller, W., & Courchamp, F. (2012). Impacts of climate change on the future of biodiversity. In Ecology Letters (Vol. 15, Issue 4). https://doi.org/10.1111/j.1461-0248.2011.01736.x Bilen, S., Bilen, M., & Bardhan, S. (2011). The effects of boron management on soil microbial population and enzyme activities. African Journal of Biotechnology, 10(27). Bonilla, C., Gómez, E., & Sánchez, M. (2002). El suelo: los organismos que lo habitan. Cuadernos Ambientales de La Universidad Nacional de Colombia, 5. Brewer, T. E., Handley, K. M., Carini, P., Gilbert, J. A., & Fierer, N. (2016). Genome reduction in an abundant and ubiquitous soil bacterium “Candidatus Udaeobacter copiosus.” Nature Microbiology, 2. https://doi.org/10.1038/nmicrobiol.2016.198 Caporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., Costello, E. K., Fierer, N., Pẽa, A. G., Goodrich, J. K., Gordon, J. I., Huttley, G. A., Kelley, S. T., Knights, D., Koenig, J. E., Ley, R. E., Lozupone, C. A., McDonald, D., Muegge, B. D., Pirrung, M., … Knight, R. (2010). QIIME allows analysis of high-throughput community sequencing data. In Nature Methods (Vol. 7, Issue 5). https://doi.org/10.1038/nmeth.f.303 Castaño, C. (2002). Páramos y ecosistemas altoandinos de Colombia en condición hotspot y global climatic tensor. IDEAM, Bogotá. Cleveland, C. C., Nemergut, D. R., Schmidt, S. K., & Townsend, A. R. (2007). Increases in soil respiration following labile carbon additions linked to rapid shifts in soil microbial community composition. Biogeochemistry, 82(3). https://doi.org/10.1007/s10533-006-9065-z Daims, H., Lebedeva, E. v., Pjevac, P., Han, P., Herbold, C., Albertsen, M., Jehmlich, N., Palatinszky, M., Vierheilig, J., Bulaev, A., Kirkegaard, R. H., von Bergen, M., Rattei, T., Bendinger, B., Nielsen, P. H., & Wagner, M. (2015). Complete nitrification by Nitrospira bacteria. Nature, 528(7583). https://doi.org/10.1038/nature16461 DEFORESTACIÓN EN COLOMBIA - IDEAM. (s/f). Gov.co. Recuperado el 1 de febrero de 2022, de http://www.ideam.gov.co/web/bosques/deforestacion-colombia Dobbelaere, S., Vanderleyden, J., & Okon, Y. (2003). Plant growth-promoting effects of diazotrophs in the rhizosphere. In Critical Reviews in Plant Sciences (Vol. 22, Issue 2). https://doi.org/10.1080/713610853 dos Banhos, E. F., de Souza, A. Q. L., de Andrade, J. C., de Souza, A. D. L., Koolen, H. H. F., & Albuquerque, P. M. (2014). Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: Distribution and bioactivity. Brazilian Journal of Microbiology, 45(1). https://doi.org/10.1590/S1517-83822014005000027 Dunbar, J., Barns, S. M., Ticknor, L. O., & Kuske, C. R. (2002). Empirical and theoretical bacterial diversity in four Arizona soils. Applied and Environmental Microbiology, 68(6). https://doi.org/10.1128/AEM.68.6.3035-3045.2002 Dworkin, M., & Falkow, S. (2006). The Prokaryotes: Ecophysiology and biochemistry. In Springer (Vol. 2). Eaton, W. D., & Hamilton, D. A. (2022). Enhanced carbon, nitrogen and associated bacterial community compositional complexity, stability, evenness, and differences within the tree-soils of Inga punctata along an age gradient of planted trees in reforestation plots. Plant and Soil. https://doi.org/10.1007/s11104-022-05793-8 Francioli, D., Schulz, E., Lentendu, G., Wubet, T., Buscot, F., and Reitz, T. (2016). Mineral vs. organic amendments: microbial community structure, activity and abundance of agriculturally relevant microbes are driven by long-term fertilization strategies. Front. Microbiol. 7:1446. FAO. (2015). Estado mundial del recurso del suelo (EMRS) - Resumen Tecnico. In Fao. Fierer, N., Bradford, M. A., & Jackson, R. B. (2007). Toward an ecological classification of soil bacteria. Ecology, 88(6), 1354–1364. https://doi.org/10.1890/05-1839 Galindo, G., Cabrera, E., Otero, J., Bernal, N.R., & Palacios, S. (2009). Planificación Ecorregional Para La Conservación De La Biodiversidad En Los Andes Y El Piedemonte Amazónico Colombianos. Nat. Conserv. e Inst. Hidrol Meteorol. y Estud. Ambient, 2, 24. Garavito, N. (2016). Los páramos en Colombia, un ecosistema en riesgo. Ingeniare, 19. Geml, J., Pastor, N., Fernandez, L., Pacheco, S., Semenova, T. A., Becerra, A. G., Wicaksono, C. Y., & Nouhra, E. R. (2014). Large-scale fungal diversity assessment in the Andean Yungas forests reveals strong community turnover among forest types along an altitudinal gradient. Molecular Ecology, 23(10), 2452–2472. https://doi.org/10.1111/mec.12765 Giri, S., & Pati, B. R. (2004). A comparative study on phyllosphere nitrogen fixation by newly isolated Corynebacterium sp. & Flavobacterium sp. and their potentialities as biofertilizer. Acta Microbiologica et Immunologica Hungarica, 51(1–2). https://doi.org/10.1556/AMicr.51.2004.1-2.3 Gómez-Rubio, V. (2017). ggplot2 - Elegant Graphics for Data Analysis (2nd Edition) . Journal of Statistical Software, 77(Book Review 2). https://doi.org/10.18637/jss.v077.b02 González, A., Cárdenas, M., & Restrepo, S. (2012). Metagenómica: Revelación de comunidades microbianas. Hipótesis, Apuntes Científicos Uniandinos, 12. Hjelmsø, M. H., Hansen, L. H., Bælum, J., Feld, L., Holben, W. E., & Jacobsen, C. S. (2014). High-resolution melt analysis for rapid comparison of bacterial community compositions. Applied and Environmental Microbiology, 80(12). https://doi.org/10.1128/AEM.03923-13 Huang, S., Bao, J., Shan, M., Qin, H., Wang, H., Yu, X., Chen, J., & Xu, Q. (2018). Dynamic changes of polychlorinated biphenyls (PCBs) degradation and adsorption to biochar as affected by soil organic carbon content. Chemosphere, 211. https://doi.org/10.1016/j.chemosphere.2018.07.133 Jacoby, R., Peukert, M., Succurro, A., Koprivova, A., & Kopriva, S. (2017). The role of soil microorganisms in plant mineral nutrition—current knowledge and future directions. Frontiers in Plant Science, 8. https://doi.org/10.3389/fpls.2017.01617 Janssen, P. H. (2006). Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes. In Applied and Environmental Microbiology (Vol. 72, Issue 3). https://doi.org/10.1128/AEM.72.3.1719-1728.2006 Ji, Y., Ashton, L., Pedley, S. M., Edwards, D. P., Tang, Y., Nakamura, A., Kitching, R., Dolman, P. M., Woodcock, P., Edwards, F. A., Larsen, T. H., Hsu, W. W., Benedick, S., Hamer, K. C., Wilcove, D. S., Bruce, C., Wang, X., Levi, T., Lott, M., … Yu, D. W. (2013). Reliable, verifiable and efficient monitoring of biodiversity via metabarcoding. Ecology Letters, 16(10). https://doi.org/10.1111/ele.12162 Kattán, G. (2003). Bosques Andinos y Subandinos del departamento del Valle de Cauca,Colombia. Corporación Autónoma Regional del Valle del Cauca. Santiago de Cali, Colombia. Keller, I., Alexander, J. M., Holderegger, R., & Edwards, P. J. (2013). Widespread phenotypic and genetic divergence along altitudinal gradients in animals. In Journal of Evolutionary Biology (Vol. 26, Issue 12). https://doi.org/10.1111/jeb.12255 Kielak, A. M., Barreto, C. C., Kowalchuk, G. A., van Veen, J. A., & Kuramae, E. E. (2016). The ecology of Acidobacteria: Moving beyond genes and genomes. In Frontiers in Microbiology (Vol. 7, Issue MAY). Frontiers Media S.A. https://doi.org/10.3389/fmicb.2016.00744 Lauber, C. L., Hamady, M., Knight, R., & Fierer, N. (2009). Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale. Applied and Environmental Microbiology, 75(15). https://doi.org/10.1128/AEM.00335-09 Lauber, C. L., Strickland, M. S., Bradford, M. A., & Fierer, N. (2008). The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biology and Biochemistry, 40(9). https://doi.org/10.1016/j.soilbio.2008.05.021 Liang, S., Deng, J., Jiang, Y., Wu, S., Zhou, Y., & Zhu, W. (2020). Functional distribution of bacterial community under different land use patterns based on faprotax function prediction. Polish Journal of Environmental Studies, 29(2). https://doi.org/10.15244/pjoes/108510 Lin, H. F., Xiong, J., Zhou, H. M., Chen, C. M., Lin, F. Z., Xu, X. M., Oelmüller, R., Xu, W. F., & Yeh, K. W. (2019). Growth promotion and disease resistance induced in Anthurium colonized by the beneficial root endophyte Piriformospora indica. BMC Plant Biology, 19(1). https://doi.org/10.1186/s12870-019-1649-6 López, A. J. (2006). MANUAL DE EDAFOLOGÍA. Media, 806 (Enero). Louca, S., Parfrey, L. W., & Doebeli, M. (2016). Decoupling function and taxonomy in the global ocean microbiome. Science, 353(6305). https://doi.org/10.1126/science.aaf4507 Love, M. I., Huber, W., & Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15(12). https://doi.org/10.1186/s13059-014-0550-8 Lundell, T. K., Mäkelä, M. R., & Hildén, K. (2010). Lignin-modifying enzymes in filamentous basidiomycetes - Ecological, functional and phylogenetic review. In Journal of Basic Microbiology (Vol. 50, Issue 1). https://doi.org/10.1002/jobm.200900338 Mantilla-Paredes, A. J., Cardona, G. I., Peña-Venegas, C. P., Murcia, U., Rodríguez, M., & Zambrano, M. M. (2009). Distribución de bacterias potencialmente fijadoras de nitrógeno y su relación con parámetros fisicoquímicos en suelos con tres coberturas vegetales en el sur de la Amazonia colombiana. Revista de Biologia Tropical, 57(4). https://doi.org/10.15517/rbt.v57i4.5436 McMurdie, P. J., & Holmes, S. (2013). Phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data. PLoS ONE, 8(4). https://doi.org/10.1371/journal.pone.0061217 Meng, H., Li, K., Nie, M., Wan, J. R., Quan, Z. X., Fang, C. M., Chen, J. K., Gu, J. D., & Li, B. (2013). Responses of bacterial and fungal communities to an elevation gradient in a subtropical montane forest of China. Applied Microbiology and Biotechnology, 97(5). https://doi.org/10.1007/s00253-012-4063-7 Menkis, A., Urbina, H., James, T. Y., & Rosling, A. (2014). Archaeorhizomyces borealis sp. nov. and a sequence-based classification of related soil fungal species. Fungal Biology, 118(12), 943–955. https://doi.org/10.1016/j.funbio.2014.08.005 Michalet, R., Schöb, C., Lortie, C. J., Brooker, R. W., & Callaway, R. M. (2014). Partitioning net interactions among plants along altitudinal gradients to study community responses to climate change. Functional Ecology, 28(1). https://doi.org/10.1111/1365-2435.12136 Morales, J., Van der Hammen, T., Torres A., C. C., C., P., Rodríguez N., F. C., J.C., B., Olaya E., P. E., & L., C. (2007). Atlas de Páramos de Colombia. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt., 1. Morrissey, E. M., Mau, R. L., Schwartz, E., McHugh, T. A., Dijkstra, P., Koch, B. J., Marks, J. C., & Hungate, B. A. (2017). Bacterial carbon use plasticity, phylogenetic diversity and the priming of soil organic matter. ISME Journal, 11(8). https://doi.org/10.1038/ismej.2017.43 Navarrete, A. A., Kuramae, E. E., de Hollander, M., Pijl, A. S., van Veen, J. A., & Tsai, S. M. (2013). Acidobacterial community responses to agricultural management of soybean in Amazon forest soils. FEMS Microbiology Ecology, 83(3), 607–621. https://doi.org/10.1111/1574-6941.12018 Nguyen, N. H., Song, Z., Bates, S. T., Branco, S., Tedersoo, L., Menke, J., Schilling, J. S., & Kennedy, P. G. (2016). FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology, 20. https://doi.org/10.1016/j.funeco.2015.06.006 Pacheco Flores de Valgaz, A., Barcos-Arias, M., Naranjo-Morán, J., Peña Tapia, D., & Moreira-Gómez, R. (2022). Ericaceous Plants: A Review for the Bioprospecting of Ericoid Mycorrhizae from Ecuador. In Diversity (Vol. 14, Issue 8). MDPI. https://doi.org/10.3390/d14080648 Patiño López, C. O., & Sanclemente Reyes, O. E. (2014). Los microorganismos solubilizadores de fósforo (MSF): Una alternativa biotecnólogica para una agricultura sostenible. Entramado, 10(2). Pessoa-Filho, M., Barreto, C. C., dos Reis Junior, F. B., Fragoso, R. R., Costa, F. S., de Carvalho Mendes, I., & de Andrade, L. R. M. (2015). Microbiological functioning, diversity, and structure of bacterial communities in ultramafic soils from a tropical savanna. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 107(4), 935–949. https://doi.org/10.1007/S10482-015-0386-6/METRICS Peters, M. K., Hemp, A., Appelhans, T., Becker, J. N., Behler, C., Classen, A., Detsch, F., Ensslin, A., Ferger, S. W., Frederiksen, S. B., Gebert, F., Gerschlauer, F., Gütlein, A., Helbig-Bonitz, M., Hemp, C., Kindeketa, W. J., Kühnel, A., Mayr, A. V., Mwangomo, E., … Steffan-Dewenter, I. (2019). Climate–land-use interactions shape tropical mountain biodiversity and ecosystem functions. Nature, 568(7750). https://doi.org/10.1038/s41586-019-1048-z Pievani, T. (2014). The sixth mass extinction: Anthropocene and the human impact on biodiversity. Rendiconti Lincei, 25(1). https://doi.org/10.1007/s12210-013-0258-9 Pimm, S. L., & Raven, P. (2000). Biodiversity. Extinction by numbers. Nature, 403(6772). https://doi.org/10.1038/35002708 Pinto-Figueroa, E. A., Seddon, E., Yashiro, E., Buri, A., Niculita-Hirzel, H., Van Der Meer, J. R., & Guisan, A. (2019). Archaeorhizomycetes Spatial Distribution in Soils along Wide Elevational and Environmental Gradients Reveal Co-abundance Patterns with Other Fungal Saprobes and Potential Weathering Capacities. Frontiers in Microbiology, 10(APR). https://doi.org/10.3389/fmicb.2019.00656 Pizano, C., & García, H. (2014). El bosque seco tropical en Colombia. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt (IAvH). Bogotá, D.C., Colombia. Fundación CIPAV, 1822(September). Pizano, C., González-M., R., López, R., Jurado, R. D., Cuadros, H., Castaño-Naranjo, A., Rojas, A., Peréz, K., Vergara-Varela, H., Idárraga, Á., Isaacs, P., & García, H. (2016). El bosque seco tropical en Colombia. In Biodiversidad 2015. Estado y tendencias de la biodiversidad continental de Colombia. https://doi.org/10.21068/b001.2015.202 Quintero, Benavides, A. M., Moreno, N. & Gonzales, S. (2017). BOSQUES ANDINOS, Estado actual y retos para su conservación en Antioquía. Fundación Jardín Botánico de Medellín Joaquín Antonio Uribe, Programa Bosques Andinos (COSUDE). Medellín, Antioquia. R Core Team. (2022). R Core Team 2021 R: A language and environment for statistical computing. R foundation for statistical computing. https://www.R-project.org/. R Foundation for Statistical Computing, 2. Rahbek, C. (2005). The role of spatial scale and the perception of large-scale species-richness patterns. In Ecology Letters (Vol. 8, Issue 2). https://doi.org/10.1111/j.1461-0248.2004.00701.x Ramírez-Carvajal, R. (1997). Propiedades físicas, químicas y biológicas del suelo. Convenio FENALCE-SENA-SAC, 9–23. Ramírez, C., Duarte, C., & Galeano, J. (2010). Estudio de suelos y su relación con las plantas en el páramo el verjón ubicado en el municipio de choachí cundinamarca. Tecciencia, 6(12). Rawat, S. R., Männistö, M. K., Bromberg, Y., & Häggblom, M. M. (2012). Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils. FEMS Microbiology Ecology, 82(2). https://doi.org/10.1111/j.1574-6941.2012.01381.x Ren, C., Zhang, W., Zhong, Z. K., Han, X., Yang, G., Feng, Y., & Ren, G. (2018). Differential responses of soil microbial biomass, diversity, and compositions to altitudinal gradients depend on plant and soil characteristics. Science of the Total Environment, 610–611, 750–758. https://doi.org/10.1016/j.scitotenv.2017.08.110 Ren, C., Zhou, Z., Guo, Y., Yang, G., Zhao, F., Wei, G., Han, X., Feng, L., Feng, Y., & Ren, G. (2021). Contrasting patterns of microbial community and enzyme activity between rhizosphere and bulk soil along an elevation gradient. Catena, 196. https://doi.org/10.1016/j.catena.2020.104921 Riechers, M., Balázsi, Á., Betz, L., Jiren, T. S., & Fischer, J. (2020). The erosion of relational values resulting from landscape simplification. Landscape Ecology, 35(11). https://doi.org/10.1007/s10980-020-01012-w Romaniuk, R. I., Venece, M., Cosentino, V. R. N., Alvarez, C. R., Ciarlo, E. A., Rimski Korsakov, H., Steinbach, H. S., & Lupi, A. M. (2021). Dinámica del carbono lábil del suelo en sistemas forestales de Eucalyptus grandis Hill ex Maiden en la Mesopotamia Argentina. Bosque (Valdivia), 42(3). https://doi.org/10.4067/s0717-92002021000300343 Rosling, A., Cox, F., Cruz-Martinez, K., Ihrmark, K., Grelet, G. A., Lindahl, B. D., Menkis, A., & James, T. Y. (2011). Archaeorhizomycetes: Unearthing an ancient class of ubiquitous soil fungi. Science, 333(6044). https://doi.org/10.1126/science.1206958 Ruiz-Pérez, C. A., Restrepo, S., & Zambrano, M. M. (2016). Microbial and functional diversity within the phyllosphere of Espeletia species in an Andean high-mountain ecosystem. Applied and Environmental Microbiology, 82(6). https://doi.org/10.1128/AEM.02781-15 Shen, C., Ge, Y., Yang, T., & Chu, H. (2017). Verrucomicrobial elevational distribution was strongly influenced by soil pH and carbon/nitrogen ratio. Journal of Soils and Sediments, 17(10). https://doi.org/10.1007/s11368-017-1680-x Shen, C., Shi, Y., Fan, K., He, J. S., Adams, J. M., Ge, Y., & Chu, H. (2019). Soil pH dominates elevational diversity pattern for bacteria in high elevation alkaline soils on the Tibetan Plateau. FEMS Microbiology Ecology, 95(2). https://doi.org/10.1093/femsec/fiz003 Siles, J. A., & Margesin, R. (2016). Abundance and Diversity of Bacterial, Archaeal, and Fungal Communities Along an Altitudinal Gradient in Alpine Forest Soils: What Are the Driving Factors? Microbial Ecology, 72(1), 207–220. https://doi.org/10.1007/s00248-016-0748-2 Singh, D., Takahashi, K., Kim, M., Chun, J., & Adams, J. M. (2012). A Hump-Backed Trend in Bacterial Diversity with Elevation on Mount Fuji, Japan. Microbial Ecology, 63(2), 429–437. https://doi.org/10.1007/s00248-011-9900-1 St. Leger, R. J., & Wang, J. B. (2020). Metarhizium: jack of all trades, master of many. Open Biology, 10(12). https://doi.org/10.1098/rsob.200307 Świeciło, A., & Zych-Wezyk, I. (2013). Bacterial stress response as an adaptation to life in a soil environment. In Polish Journal of Environmental Studies (Vol. 22, Issue 6). The jamovi. (2022). The Jamovi Project (Version 2.3) [Computer Software]. In Retrieved from https://www.jamovi.org. Toro, D. (2004). LA BIODIVERSIDAD MICROBIANA DEL SUELO, UN MUNDO POR DESCUBRIR. Lunazul Uniacaldas, 1(1). Valderrama Ardila, C. H. (2012). Estado de fragmentación del bosque seco de la cuenca alta del río Cauca, Colombia. Biota Colombiana, 13(2). Vallejo, V. (2013). Importancia y utilidad de la evaluación de la calidad de suelos mediante el componente microbiano: experiencias en sistemas silvopastoriles. Colombia Forestal, 16(1). Větrovský, T., Kohout, P., Kopecký, M., Machac, A., Man, M., Bahnmann, B. D., Brabcová, V., Choi, J., Meszárošová, L., Human, Z. R., Lepinay, C., Lladó, S., López-Mondéjar, R., Martinović, T., Mašínová, T., Morais, D., Navrátilová, D., Odriozola, I., Štursová, M., … Baldrian, P. (2019). A meta-analysis of global fungal distribution reveals climate-driven patterns. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-13164-8 Viitamäki, S., Pessi, I. S., Virkkala, A.-M., Niittynen, P., Kemppinen, J., Eronen-Rasimus, E., Luoto, M., & Hultman, J. (2022). The activity and functions of soil microbial communities in the Finnish sub-Arctic vary across vegetation types. FEMS Microbiology Ecology, 98(8). https://doi.org/10.1093/femsec/fiac079 Wang, J., Soininen, J., Zhang, Y., Wang, B., Yang, X., & Shen, J. (2011). Contrasting patterns in elevational diversity between microorganisms and macroorganisms. Journal of Biogeography, 38(3). https://doi.org/10.1111/j.1365-2699.2010.02423.x Willms, I. M., Bolz, S. H., Yuan, J., Krafft, L., Schneider, D., Schöning, I., Schrumpf, M., & Nacke, H. (2021). The ubiquitous soil verrucomicrobial clade ‘Candidatus Udaeobacter’ shows preferences for acidic pH. Environmental Microbiology Reports, 13(6), 878–883. https://doi.org/10.1111/1758-2229.13006 Willms, I. M., Rudolph, A. Y., Göschel, I., Bolz, S. H., Schneider, D., Penone, C., Poehlein, A., Schöning, I., & Nacke, H. (2020). Globally Abundant “ Candidatus Udaeobacter” Benefits from Release of Antibiotics in Soil and Potentially Performs Trace Gas Scavenging . MSphere, 5(4). https://doi.org/10.1128/msphere.00186-20 WWF-Colombia. (2017). Colombia viva: Un país megadiverso de cara al futuro. Informe 2017. 165. Yang, N., Li, X., Liu, D., Zhang, Y., Chen, Y., Wang, B., Hua, J., Zhang, J., Peng, S., Ge, Z., Li, J., Ruan, H., & Mao, L. (2022). Diversity patterns and drivers of soil bacterial and fungal communities along elevational gradients in the Southern Himalayas, China. Applied Soil Ecology, 178. https://doi.org/10.1016/j.apsoil.2022.104563 Zhang, Y., Heal, K. V., Shi, M., Chen, W., & Zhou, C. (2022). Decreasing molecular diversity of soil dissolved organic matter related to microbial community along an alpine elevation gradient. Science of the Total Environment, 818. https://doi.org/10.1016/j.scitotenv.2021.151823 Zou, X. M., Ruan, H. H., Fu, Y., Yang, X. D., & Sha, L. Q. (2005). Estimating soil labile organic carbon and potential turnover rates using a sequential fumigation-incubation procedure. Soil Biology and Biochemistry, 37(10). https://doi.org/10.1016/j.soilbio.2005.02.028
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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.uri.spa.fl_str_mv	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.accessrights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
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dc.format.extent.spa.fl_str_mv	xii, 81 páginas + anexos
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dc.coverage.region.none.fl_str_mv	Valle del Cauca, Colombia
dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Palmira - Ciencias Agropecuarias - Maestría en Ciencias Biológicas
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias Agropecuarias
dc.publisher.place.spa.fl_str_mv	Palmira, Valle del Cauca, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Palmira
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Muñoz Flórez, Jaime Eduardo21cc0d0661318d1e84ac9a225a0fbdc5Rugeles Silva, Paula Andrea2c2e97576e44a4f4259bc1916adc4552Vélez Martínez, Glever Alexanderfe9375febdf749041c7df3ed4733e44aGrupo de Investigación en Diversidad BiológicaVelez Martinez, Glever Alexander [0009-0002-9029-0154]Muñoz Flórez, Jaime Eduardo [0000-0002-8237-0499]Rugeles Silva, Paula Andrea [0000-0002-7419-6638]2023-06-05T16:51:04Z2023-06-05T16:51:04Z2023-04-20https://repositorio.unal.edu.co/handle/unal/83966Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones, tablasLos microorganismos del suelo en bosques y páramos son ejes claves en el mantenimiento de los ecosistemas por sus actividades de descomposición, nutrición de plantas e intervención en los ciclos biogeoquímicos. En esta investigación se recolectaron muestras de suelo en cuatro localidades en la cordillera central de los Andes en el Valle del Cauca, Colombia siguiendo un gradiente altitudinal entre los 1000 y 3800 msnm. Seguidamente se secuenciaron el gen 16S de ARNr para bacterias y la región ITS1 para hongos empleando metabarcoding. Los análisis de composición taxonómica de microorganismos mostraron variaciones de abundancias a través del gradiente altitudinal, siendo las bacterias Acidobacteriota y Proteobacteriota favorecidas en las mayores alturas y Actinobacteria, Firmicutes y Verrucomicrobia en las menores elevaciones. En hongos, Ascomycota tuvo asignación máxima de lecturas en la mayor elevación, Basidiomycota tendió a dominar en las dos menores altitudes y Mortierellomycota reportó la mayoría de sus lecturas en elevaciones intermedias. Las tendencias de esas clasificaciones presentaron relaciones con parámetros fisicoquímicos del suelo como el carbono orgánico, nitrógeno y pH. El índice de Shannon indicó alta diversidad de bacterias y hongos (>3) en todas las localidades, presentándose un patrón de “U” invertida con máximos de diversidad en la reserva el Pailón a 2400 msnm. Además, se evidenciaron asociaciones de géneros de microorganismos con plantas dentro de los ecosistemas y anotaciones funcionales predictivas diferenciales a través el gradiente. Concluimos que las comunidades de bacterias y hongos del suelo difirieron en estructura y diversidad conforme variaron factores preponderantes para los microorganismos edáficos. (Texto tomado de la fuente)Soil microorganisms in forests and páramos are key axes in the maintenance of ecosystems due to their decomposition activities, plant nutrition and intervention in biogeochemical cycles. In this research, soil samples were collected in four localities in the central Andes mountain range in Valle del Cauca, Colombia, following an altitudinal gradient between 1000 and 3800 masl. The 16S rRNA gene for bacteria and the ITS1 region for fungi were sequenced using metabarcoding. Analysis of the taxonomic composition of microorganisms showed variations in abundance across the altitudinal gradient, with Acidobacteriota and Proteobacteriota being favored at higher altitudes and Actinobacteria, Firmicutes and Verrucomicrobia at lower elevations. In fungi, Ascomycota was more abundant at higher elevations, Basidiomycota tended to dominate at lower elevations and Mortierellomycota reported most of their readings at intermediate elevations. Trends in these classifications showed relationships with soil physicochemical parameters such as organic carbon, nitrogen and pH. The Shannon index indicated high diversity of bacteria and fungi (>3) in all localities, presenting an inverted "U" pattern with maximum diversity in the Pailón reserve at 2400 masl. In addition, associations of microorganism genera with plants within the ecosystems and differential predictive functional annotations across the gradient were evidenced. We conclude that soil bacterial and fungal communities differed in structure and diversity as factors preponderant for edaphic microorganisms varied.MaestríaLas localidades del estudio fueron el parque natural regional El Vínculo (bosque seco tropical, 1000 msnm), el parque natural regional Mateguadua (bosque seco tropical, 1200 msnm), la reserva de la sociedad civil El Pailón (bosque andino, 2400 msnm) y distrito regional de manejo integrado páramo Las Domínguez (páramo, 3800 msnm) (Figura 1). En cada localidad se conformaron tres parcelas de 10 m x 10 m, separadas entre sí por 20 metros (12 parcelas en total). Para análisis microbiano, de cada parcela se obtuvieron tres muestras de suelo en tubos falcon de 15 mL de los primeros 25 centímetros de profundidad (36 muestras en total). Además, se recolectaron tres muestras de un kilogramo de suelo en los mismos puntos para análisis fisicoquímico y se realizó una caracterización taxonómica de las plantas presentes en el área de las parcelas. Las muestras de suelo se transportaron al laboratorio de Biología Molecular de la Universidad Nacional de Colombia sede Palmira, en donde se mantuvo el suelo para el análisis microbiano a -80 °C hasta la extracción del ADNCiencias Agropecuarias.Sede Palmiraxii, 81 páginas + anexosapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ciencias Agropecuarias - Maestría en Ciencias BiológicasFacultad de Ciencias AgropecuariasPalmira, Valle del Cauca, ColombiaUniversidad Nacional de Colombia - Sede Palmira570 - Biología::577 - EcologíaRNA sequenceSecuencia de ARNNext Generation SequencingMicrobial CommunitiesMetagenomicsColombian terrestrial ecosystemsMeta-taxonomyMicrobial ecologyAltitudinal gradientEcosistemas terrestres colombianosMeta-taxonomíaEcología microbianaGradiente altitudinalEcosistemas terrestresTerrestrial ecosystemsMetabarcoding de comunidades microbianas del suelo en ecosistemas de bosques y páramo en el Valle del CaucaMetabarcoding of soil microbial communities in forest and páramo ecosystems in Valle del Cauca.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMValle del Cauca, ColombiaAshraf, M., Hussain, M., Ahmad, M. S. A., Al-Qurainy, F., & Hameed, M. (2012). Strategies for conservation of endangered ecosystems. Pakistan Journal of Botany, 44 (SPL. ISS. 2).Bayranvand, M., Akbarinia, M., Salehi Jouzani, G., Gharechahi, J., Kooch, Y., & Baldrian, P. (2021). Composition of soil bacterial and fungal communities in relation to vegetation composition and soil characteristics along an altitudinal gradient. FEMS Microbiology Ecology, 97(1). https://doi.org/10.1093/femsec/fiaa201Bellard, C., Bertelsmeier, C., Leadley, P., Thuiller, W., & Courchamp, F. (2012). Impacts of climate change on the future of biodiversity. In Ecology Letters (Vol. 15, Issue 4). https://doi.org/10.1111/j.1461-0248.2011.01736.xBilen, S., Bilen, M., & Bardhan, S. (2011). The effects of boron management on soil microbial population and enzyme activities. African Journal of Biotechnology, 10(27).Bonilla, C., Gómez, E., & Sánchez, M. (2002). El suelo: los organismos que lo habitan. Cuadernos Ambientales de La Universidad Nacional de Colombia, 5.Brewer, T. E., Handley, K. M., Carini, P., Gilbert, J. A., & Fierer, N. (2016). Genome reduction in an abundant and ubiquitous soil bacterium “Candidatus Udaeobacter copiosus.” Nature Microbiology, 2. https://doi.org/10.1038/nmicrobiol.2016.198Caporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., Costello, E. K., Fierer, N., Pẽa, A. G., Goodrich, J. K., Gordon, J. I., Huttley, G. A., Kelley, S. T., Knights, D., Koenig, J. E., Ley, R. E., Lozupone, C. A., McDonald, D., Muegge, B. D., Pirrung, M., … Knight, R. (2010). QIIME allows analysis of high-throughput community sequencing data. In Nature Methods (Vol. 7, Issue 5). https://doi.org/10.1038/nmeth.f.303Castaño, C. (2002). Páramos y ecosistemas altoandinos de Colombia en condición hotspot y global climatic tensor. IDEAM, Bogotá.Cleveland, C. C., Nemergut, D. R., Schmidt, S. K., & Townsend, A. R. (2007). Increases in soil respiration following labile carbon additions linked to rapid shifts in soil microbial community composition. Biogeochemistry, 82(3). https://doi.org/10.1007/s10533-006-9065-zDaims, H., Lebedeva, E. v., Pjevac, P., Han, P., Herbold, C., Albertsen, M., Jehmlich, N., Palatinszky, M., Vierheilig, J., Bulaev, A., Kirkegaard, R. H., von Bergen, M., Rattei, T., Bendinger, B., Nielsen, P. H., & Wagner, M. (2015). Complete nitrification by Nitrospira bacteria. Nature, 528(7583). https://doi.org/10.1038/nature16461DEFORESTACIÓN EN COLOMBIA - IDEAM. (s/f). Gov.co. Recuperado el 1 de febrero de 2022, de http://www.ideam.gov.co/web/bosques/deforestacion-colombiaDobbelaere, S., Vanderleyden, J., & Okon, Y. (2003). Plant growth-promoting effects of diazotrophs in the rhizosphere. In Critical Reviews in Plant Sciences (Vol. 22, Issue 2). https://doi.org/10.1080/713610853dos Banhos, E. F., de Souza, A. Q. L., de Andrade, J. C., de Souza, A. D. L., Koolen, H. H. F., & Albuquerque, P. M. (2014). Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: Distribution and bioactivity. Brazilian Journal of Microbiology, 45(1). https://doi.org/10.1590/S1517-83822014005000027Dunbar, J., Barns, S. M., Ticknor, L. O., & Kuske, C. R. (2002). Empirical and theoretical bacterial diversity in four Arizona soils. Applied and Environmental Microbiology, 68(6). https://doi.org/10.1128/AEM.68.6.3035-3045.2002Dworkin, M., & Falkow, S. (2006). The Prokaryotes: Ecophysiology and biochemistry. In Springer (Vol. 2).Eaton, W. D., & Hamilton, D. A. (2022). Enhanced carbon, nitrogen and associated bacterial community compositional complexity, stability, evenness, and differences within the tree-soils of Inga punctata along an age gradient of planted trees in reforestation plots. Plant and Soil. https://doi.org/10.1007/s11104-022-05793-8Francioli, D., Schulz, E., Lentendu, G., Wubet, T., Buscot, F., and Reitz, T. (2016). Mineral vs. organic amendments: microbial community structure, activity and abundance of agriculturally relevant microbes are driven by long-term fertilization strategies. Front. Microbiol. 7:1446.FAO. (2015). Estado mundial del recurso del suelo (EMRS) - Resumen Tecnico. In Fao.Fierer, N., Bradford, M. A., & Jackson, R. B. (2007). Toward an ecological classification of soil bacteria. Ecology, 88(6), 1354–1364. https://doi.org/10.1890/05-1839Galindo, G., Cabrera, E., Otero, J., Bernal, N.R., & Palacios, S. (2009). Planificación Ecorregional Para La Conservación De La Biodiversidad En Los Andes Y El Piedemonte Amazónico Colombianos. Nat. Conserv. e Inst. Hidrol Meteorol. y Estud. Ambient, 2, 24.Garavito, N. (2016). Los páramos en Colombia, un ecosistema en riesgo. Ingeniare, 19.Geml, J., Pastor, N., Fernandez, L., Pacheco, S., Semenova, T. A., Becerra, A. G., Wicaksono, C. Y., & Nouhra, E. R. (2014). Large-scale fungal diversity assessment in the Andean Yungas forests reveals strong community turnover among forest types along an altitudinal gradient. Molecular Ecology, 23(10), 2452–2472. https://doi.org/10.1111/mec.12765Giri, S., & Pati, B. R. (2004). A comparative study on phyllosphere nitrogen fixation by newly isolated Corynebacterium sp. & Flavobacterium sp. and their potentialities as biofertilizer. Acta Microbiologica et Immunologica Hungarica, 51(1–2). https://doi.org/10.1556/AMicr.51.2004.1-2.3Gómez-Rubio, V. (2017). ggplot2 - Elegant Graphics for Data Analysis (2nd Edition) . Journal of Statistical Software, 77(Book Review 2). https://doi.org/10.18637/jss.v077.b02González, A., Cárdenas, M., & Restrepo, S. (2012). Metagenómica: Revelación de comunidades microbianas. Hipótesis, Apuntes Científicos Uniandinos, 12.Hjelmsø, M. H., Hansen, L. H., Bælum, J., Feld, L., Holben, W. E., & Jacobsen, C. S. (2014). High-resolution melt analysis for rapid comparison of bacterial community compositions. Applied and Environmental Microbiology, 80(12). https://doi.org/10.1128/AEM.03923-13Huang, S., Bao, J., Shan, M., Qin, H., Wang, H., Yu, X., Chen, J., & Xu, Q. (2018). Dynamic changes of polychlorinated biphenyls (PCBs) degradation and adsorption to biochar as affected by soil organic carbon content. Chemosphere, 211. https://doi.org/10.1016/j.chemosphere.2018.07.133Jacoby, R., Peukert, M., Succurro, A., Koprivova, A., & Kopriva, S. (2017). The role of soil microorganisms in plant mineral nutrition—current knowledge and future directions. Frontiers in Plant Science, 8. https://doi.org/10.3389/fpls.2017.01617Janssen, P. H. (2006). Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes. In Applied and Environmental Microbiology (Vol. 72, Issue 3). https://doi.org/10.1128/AEM.72.3.1719-1728.2006Ji, Y., Ashton, L., Pedley, S. M., Edwards, D. P., Tang, Y., Nakamura, A., Kitching, R., Dolman, P. M., Woodcock, P., Edwards, F. A., Larsen, T. H., Hsu, W. W., Benedick, S., Hamer, K. C., Wilcove, D. S., Bruce, C., Wang, X., Levi, T., Lott, M., … Yu, D. W. (2013). Reliable, verifiable and efficient monitoring of biodiversity via metabarcoding. Ecology Letters, 16(10). https://doi.org/10.1111/ele.12162Kattán, G. (2003). Bosques Andinos y Subandinos del departamento del Valle de Cauca,Colombia. Corporación Autónoma Regional del Valle del Cauca. Santiago de Cali, Colombia.Keller, I., Alexander, J. M., Holderegger, R., & Edwards, P. J. (2013). Widespread phenotypic and genetic divergence along altitudinal gradients in animals. In Journal of Evolutionary Biology (Vol. 26, Issue 12). https://doi.org/10.1111/jeb.12255Kielak, A. M., Barreto, C. C., Kowalchuk, G. A., van Veen, J. A., & Kuramae, E. E. (2016). The ecology of Acidobacteria: Moving beyond genes and genomes. In Frontiers in Microbiology (Vol. 7, Issue MAY). Frontiers Media S.A. https://doi.org/10.3389/fmicb.2016.00744Lauber, C. L., Hamady, M., Knight, R., & Fierer, N. (2009). Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale. Applied and Environmental Microbiology, 75(15). https://doi.org/10.1128/AEM.00335-09Lauber, C. L., Strickland, M. S., Bradford, M. A., & Fierer, N. (2008). The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biology and Biochemistry, 40(9). https://doi.org/10.1016/j.soilbio.2008.05.021Liang, S., Deng, J., Jiang, Y., Wu, S., Zhou, Y., & Zhu, W. (2020). Functional distribution of bacterial community under different land use patterns based on faprotax function prediction. Polish Journal of Environmental Studies, 29(2). https://doi.org/10.15244/pjoes/108510Lin, H. F., Xiong, J., Zhou, H. M., Chen, C. M., Lin, F. Z., Xu, X. M., Oelmüller, R., Xu, W. F., & Yeh, K. W. (2019). Growth promotion and disease resistance induced in Anthurium colonized by the beneficial root endophyte Piriformospora indica. BMC Plant Biology, 19(1). https://doi.org/10.1186/s12870-019-1649-6López, A. J. (2006). MANUAL DE EDAFOLOGÍA. Media, 806 (Enero).Louca, S., Parfrey, L. W., & Doebeli, M. (2016). Decoupling function and taxonomy in the global ocean microbiome. Science, 353(6305). https://doi.org/10.1126/science.aaf4507Love, M. I., Huber, W., & Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15(12). https://doi.org/10.1186/s13059-014-0550-8Lundell, T. K., Mäkelä, M. R., & Hildén, K. (2010). Lignin-modifying enzymes in filamentous basidiomycetes - Ecological, functional and phylogenetic review. In Journal of Basic Microbiology (Vol. 50, Issue 1). https://doi.org/10.1002/jobm.200900338Mantilla-Paredes, A. J., Cardona, G. I., Peña-Venegas, C. P., Murcia, U., Rodríguez, M., & Zambrano, M. M. (2009). Distribución de bacterias potencialmente fijadoras de nitrógeno y su relación con parámetros fisicoquímicos en suelos con tres coberturas vegetales en el sur de la Amazonia colombiana. Revista de Biologia Tropical, 57(4). https://doi.org/10.15517/rbt.v57i4.5436McMurdie, P. J., & Holmes, S. (2013). Phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data. PLoS ONE, 8(4). https://doi.org/10.1371/journal.pone.0061217Meng, H., Li, K., Nie, M., Wan, J. R., Quan, Z. X., Fang, C. M., Chen, J. K., Gu, J. D., & Li, B. (2013). Responses of bacterial and fungal communities to an elevation gradient in a subtropical montane forest of China. Applied Microbiology and Biotechnology, 97(5). https://doi.org/10.1007/s00253-012-4063-7Menkis, A., Urbina, H., James, T. Y., & Rosling, A. (2014). Archaeorhizomyces borealis sp. nov. and a sequence-based classification of related soil fungal species. Fungal Biology, 118(12), 943–955. https://doi.org/10.1016/j.funbio.2014.08.005Michalet, R., Schöb, C., Lortie, C. J., Brooker, R. W., & Callaway, R. M. (2014). Partitioning net interactions among plants along altitudinal gradients to study community responses to climate change. Functional Ecology, 28(1). https://doi.org/10.1111/1365-2435.12136Morales, J., Van der Hammen, T., Torres A., C. C., C., P., Rodríguez N., F. C., J.C., B., Olaya E., P. E., & L., C. (2007). Atlas de Páramos de Colombia. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt., 1.Morrissey, E. M., Mau, R. L., Schwartz, E., McHugh, T. A., Dijkstra, P., Koch, B. J., Marks, J. C., & Hungate, B. A. (2017). Bacterial carbon use plasticity, phylogenetic diversity and the priming of soil organic matter. ISME Journal, 11(8). https://doi.org/10.1038/ismej.2017.43Navarrete, A. A., Kuramae, E. E., de Hollander, M., Pijl, A. S., van Veen, J. A., & Tsai, S. M. (2013). Acidobacterial community responses to agricultural management of soybean in Amazon forest soils. FEMS Microbiology Ecology, 83(3), 607–621. https://doi.org/10.1111/1574-6941.12018Nguyen, N. H., Song, Z., Bates, S. T., Branco, S., Tedersoo, L., Menke, J., Schilling, J. S., & Kennedy, P. G. (2016). FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology, 20. https://doi.org/10.1016/j.funeco.2015.06.006Pacheco Flores de Valgaz, A., Barcos-Arias, M., Naranjo-Morán, J., Peña Tapia, D., & Moreira-Gómez, R. (2022). Ericaceous Plants: A Review for the Bioprospecting of Ericoid Mycorrhizae from Ecuador. In Diversity (Vol. 14, Issue 8). MDPI. https://doi.org/10.3390/d14080648Patiño López, C. O., & Sanclemente Reyes, O. E. (2014). Los microorganismos solubilizadores de fósforo (MSF): Una alternativa biotecnólogica para una agricultura sostenible. Entramado, 10(2).Pessoa-Filho, M., Barreto, C. C., dos Reis Junior, F. B., Fragoso, R. R., Costa, F. S., de Carvalho Mendes, I., & de Andrade, L. R. M. (2015). Microbiological functioning, diversity, and structure of bacterial communities in ultramafic soils from a tropical savanna. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 107(4), 935–949. https://doi.org/10.1007/S10482-015-0386-6/METRICSPeters, M. K., Hemp, A., Appelhans, T., Becker, J. N., Behler, C., Classen, A., Detsch, F., Ensslin, A., Ferger, S. W., Frederiksen, S. B., Gebert, F., Gerschlauer, F., Gütlein, A., Helbig-Bonitz, M., Hemp, C., Kindeketa, W. J., Kühnel, A., Mayr, A. V., Mwangomo, E., … Steffan-Dewenter, I. (2019). Climate–land-use interactions shape tropical mountain biodiversity and ecosystem functions. Nature, 568(7750). https://doi.org/10.1038/s41586-019-1048-zPievani, T. (2014). The sixth mass extinction: Anthropocene and the human impact on biodiversity. Rendiconti Lincei, 25(1). https://doi.org/10.1007/s12210-013-0258-9Pimm, S. L., & Raven, P. (2000). Biodiversity. Extinction by numbers. Nature, 403(6772). https://doi.org/10.1038/35002708Pinto-Figueroa, E. A., Seddon, E., Yashiro, E., Buri, A., Niculita-Hirzel, H., Van Der Meer, J. R., & Guisan, A. (2019). Archaeorhizomycetes Spatial Distribution in Soils along Wide Elevational and Environmental Gradients Reveal Co-abundance Patterns with Other Fungal Saprobes and Potential Weathering Capacities. Frontiers in Microbiology, 10(APR). https://doi.org/10.3389/fmicb.2019.00656Pizano, C., & García, H. (2014). El bosque seco tropical en Colombia. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt (IAvH). Bogotá, D.C., Colombia. Fundación CIPAV, 1822(September).Pizano, C., González-M., R., López, R., Jurado, R. D., Cuadros, H., Castaño-Naranjo, A., Rojas, A., Peréz, K., Vergara-Varela, H., Idárraga, Á., Isaacs, P., & García, H. (2016). El bosque seco tropical en Colombia. In Biodiversidad 2015. Estado y tendencias de la biodiversidad continental de Colombia. https://doi.org/10.21068/b001.2015.202Quintero, Benavides, A. M., Moreno, N. & Gonzales, S. (2017). BOSQUES ANDINOS, Estado actual y retos para su conservación en Antioquía. Fundación Jardín Botánico de Medellín Joaquín Antonio Uribe, Programa Bosques Andinos (COSUDE). Medellín, Antioquia.R Core Team. (2022). R Core Team 2021 R: A language and environment for statistical computing. R foundation for statistical computing. https://www.R-project.org/. R Foundation for Statistical Computing, 2.Rahbek, C. (2005). The role of spatial scale and the perception of large-scale species-richness patterns. In Ecology Letters (Vol. 8, Issue 2). https://doi.org/10.1111/j.1461-0248.2004.00701.xRamírez-Carvajal, R. (1997). Propiedades físicas, químicas y biológicas del suelo. Convenio FENALCE-SENA-SAC, 9–23.Ramírez, C., Duarte, C., & Galeano, J. (2010). Estudio de suelos y su relación con las plantas en el páramo el verjón ubicado en el municipio de choachí cundinamarca. Tecciencia, 6(12).Rawat, S. R., Männistö, M. K., Bromberg, Y., & Häggblom, M. M. (2012). Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils. FEMS Microbiology Ecology, 82(2). https://doi.org/10.1111/j.1574-6941.2012.01381.xRen, C., Zhang, W., Zhong, Z. K., Han, X., Yang, G., Feng, Y., & Ren, G. (2018). Differential responses of soil microbial biomass, diversity, and compositions to altitudinal gradients depend on plant and soil characteristics. Science of the Total Environment, 610–611, 750–758. https://doi.org/10.1016/j.scitotenv.2017.08.110Ren, C., Zhou, Z., Guo, Y., Yang, G., Zhao, F., Wei, G., Han, X., Feng, L., Feng, Y., & Ren, G. (2021). Contrasting patterns of microbial community and enzyme activity between rhizosphere and bulk soil along an elevation gradient. Catena, 196. https://doi.org/10.1016/j.catena.2020.104921Riechers, M., Balázsi, Á., Betz, L., Jiren, T. S., & Fischer, J. (2020). The erosion of relational values resulting from landscape simplification. Landscape Ecology, 35(11). https://doi.org/10.1007/s10980-020-01012-wRomaniuk, R. I., Venece, M., Cosentino, V. R. N., Alvarez, C. R., Ciarlo, E. A., Rimski Korsakov, H., Steinbach, H. S., & Lupi, A. M. (2021). Dinámica del carbono lábil del suelo en sistemas forestales de Eucalyptus grandis Hill ex Maiden en la Mesopotamia Argentina. Bosque (Valdivia), 42(3). https://doi.org/10.4067/s0717-92002021000300343Rosling, A., Cox, F., Cruz-Martinez, K., Ihrmark, K., Grelet, G. A., Lindahl, B. D., Menkis, A., & James, T. Y. (2011). Archaeorhizomycetes: Unearthing an ancient class of ubiquitous soil fungi. Science, 333(6044). https://doi.org/10.1126/science.1206958Ruiz-Pérez, C. A., Restrepo, S., & Zambrano, M. M. (2016). Microbial and functional diversity within the phyllosphere of Espeletia species in an Andean high-mountain ecosystem. Applied and Environmental Microbiology, 82(6). https://doi.org/10.1128/AEM.02781-15Shen, C., Ge, Y., Yang, T., & Chu, H. (2017). Verrucomicrobial elevational distribution was strongly influenced by soil pH and carbon/nitrogen ratio. Journal of Soils and Sediments, 17(10). https://doi.org/10.1007/s11368-017-1680-xShen, C., Shi, Y., Fan, K., He, J. S., Adams, J. M., Ge, Y., & Chu, H. (2019). Soil pH dominates elevational diversity pattern for bacteria in high elevation alkaline soils on the Tibetan Plateau. FEMS Microbiology Ecology, 95(2). https://doi.org/10.1093/femsec/fiz003Siles, J. A., & Margesin, R. (2016). Abundance and Diversity of Bacterial, Archaeal, and Fungal Communities Along an Altitudinal Gradient in Alpine Forest Soils: What Are the Driving Factors? Microbial Ecology, 72(1), 207–220. https://doi.org/10.1007/s00248-016-0748-2Singh, D., Takahashi, K., Kim, M., Chun, J., & Adams, J. M. (2012). A Hump-Backed Trend in Bacterial Diversity with Elevation on Mount Fuji, Japan. Microbial Ecology, 63(2), 429–437. https://doi.org/10.1007/s00248-011-9900-1St. Leger, R. J., & Wang, J. B. (2020). Metarhizium: jack of all trades, master of many. Open Biology, 10(12). https://doi.org/10.1098/rsob.200307Świeciło, A., & Zych-Wezyk, I. (2013). Bacterial stress response as an adaptation to life in a soil environment. In Polish Journal of Environmental Studies (Vol. 22, Issue 6).The jamovi. (2022). The Jamovi Project (Version 2.3) [Computer Software]. In Retrieved from https://www.jamovi.org.Toro, D. (2004). LA BIODIVERSIDAD MICROBIANA DEL SUELO, UN MUNDO POR DESCUBRIR. Lunazul Uniacaldas, 1(1).Valderrama Ardila, C. H. (2012). Estado de fragmentación del bosque seco de la cuenca alta del río Cauca, Colombia. Biota Colombiana, 13(2).Vallejo, V. (2013). Importancia y utilidad de la evaluación de la calidad de suelos mediante el componente microbiano: experiencias en sistemas silvopastoriles. Colombia Forestal, 16(1).Větrovský, T., Kohout, P., Kopecký, M., Machac, A., Man, M., Bahnmann, B. D., Brabcová, V., Choi, J., Meszárošová, L., Human, Z. R., Lepinay, C., Lladó, S., López-Mondéjar, R., Martinović, T., Mašínová, T., Morais, D., Navrátilová, D., Odriozola, I., Štursová, M., … Baldrian, P. (2019). A meta-analysis of global fungal distribution reveals climate-driven patterns. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-13164-8Viitamäki, S., Pessi, I. S., Virkkala, A.-M., Niittynen, P., Kemppinen, J., Eronen-Rasimus, E., Luoto, M., & Hultman, J. (2022). The activity and functions of soil microbial communities in the Finnish sub-Arctic vary across vegetation types. FEMS Microbiology Ecology, 98(8). https://doi.org/10.1093/femsec/fiac079Wang, J., Soininen, J., Zhang, Y., Wang, B., Yang, X., & Shen, J. (2011). Contrasting patterns in elevational diversity between microorganisms and macroorganisms. Journal of Biogeography, 38(3). https://doi.org/10.1111/j.1365-2699.2010.02423.xWillms, I. M., Bolz, S. H., Yuan, J., Krafft, L., Schneider, D., Schöning, I., Schrumpf, M., & Nacke, H. (2021). The ubiquitous soil verrucomicrobial clade ‘Candidatus Udaeobacter’ shows preferences for acidic pH. Environmental Microbiology Reports, 13(6), 878–883. https://doi.org/10.1111/1758-2229.13006Willms, I. M., Rudolph, A. Y., Göschel, I., Bolz, S. H., Schneider, D., Penone, C., Poehlein, A., Schöning, I., & Nacke, H. (2020). Globally Abundant “ Candidatus Udaeobacter” Benefits from Release of Antibiotics in Soil and Potentially Performs Trace Gas Scavenging . MSphere, 5(4). https://doi.org/10.1128/msphere.00186-20WWF-Colombia. (2017). Colombia viva: Un país megadiverso de cara al futuro. Informe 2017. 165.Yang, N., Li, X., Liu, D., Zhang, Y., Chen, Y., Wang, B., Hua, J., Zhang, J., Peng, S., Ge, Z., Li, J., Ruan, H., & Mao, L. (2022). Diversity patterns and drivers of soil bacterial and fungal communities along elevational gradients in the Southern Himalayas, China. Applied Soil Ecology, 178. https://doi.org/10.1016/j.apsoil.2022.104563Zhang, Y., Heal, K. V., Shi, M., Chen, W., & Zhou, C. (2022). Decreasing molecular diversity of soil dissolved organic matter related to microbial community along an alpine elevation gradient. Science of the Total Environment, 818. https://doi.org/10.1016/j.scitotenv.2021.151823Zou, X. M., Ruan, H. H., Fu, Y., Yang, X. D., & Sha, L. Q. (2005). Estimating soil labile organic carbon and potential turnover rates using a sequential fumigation-incubation procedure. Soil Biology and Biochemistry, 37(10). https://doi.org/10.1016/j.soilbio.2005.02.028Relaciones Multiescalares de la Biodiversidad en Gradientes Altitudinales del Bosque Tropical, con Código: 1106-852-70306. Contrato: No. 491-2020.Ministerio de Ciencia, Tecnología e Innovación de Colombia (MINCIENCIAS), PATRIMONIO AUTÓNOMO FONDO NACIONAL DE FINANCIAMIENTO PARA LA CIENCIA, LA TECNOLOGÍA Y LA INNOVACIÓN “FRANCISCO JOSÉ DE CALDAS”EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83966/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1127585019.2023.pdf1127585019.2023.pdfapplication/pdf2946576https://repositorio.unal.edu.co/bitstream/unal/83966/2/1127585019.2023.pdf751c338c05735dda6721ec6c38c4ca55MD52THUMBNAIL1127585019.2023.pdf.jpg1127585019.2023.pdf.jpgGenerated Thumbnailimage/jpeg5340https://repositorio.unal.edu.co/bitstream/unal/83966/3/1127585019.2023.pdf.jpgf39fa90060cca0ad8cb4becac9f620b5MD53unal/83966oai:repositorio.unal.edu.co:unal/839662023-08-04 23:04:38.518Repositorio Institucional Universidad Nacional de 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