Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.

ilustraciones

Autores:
Caicedo Ruiz, Juan Daniel
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/84766
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/84766
https://repositorio.unal.edu.co/
Palabra clave:
610 - Medicina y salud::612 - Fisiología humana
Respiración de la célula
Ácidos tricarboxilicos
Respiração Celular
Tricarboxylic Acids
Ciclo de krebs
Ciclo de los ácidos tricarboxílicos
Sepsis
Choque séptico
Reprogramación metabolica
Cromatografía líquida
Krebs cycle
Tricarboxylic acid cycle
Sepsis
Septic shock
Metabolic reprogramming
Liquid chromatography
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openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_344e87699f740b99f25f51731d1112bb
oai_identifier_str oai:repositorio.unal.edu.co:unal/84766
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
dc.title.translated.eng.fl_str_mv Krebs cycle intermediates during the first 6 hours of lipopolysaccharide-induced endotoxemia in a swine sepsis model
title Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
spellingShingle Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
610 - Medicina y salud::612 - Fisiología humana
Respiración de la célula
Ácidos tricarboxilicos
Respiração Celular
Tricarboxylic Acids
Ciclo de krebs
Ciclo de los ácidos tricarboxílicos
Sepsis
Choque séptico
Reprogramación metabolica
Cromatografía líquida
Krebs cycle
Tricarboxylic acid cycle
Sepsis
Septic shock
Metabolic reprogramming
Liquid chromatography
title_short Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
title_full Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
title_fullStr Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
title_full_unstemmed Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
title_sort Análisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.
dc.creator.fl_str_mv Caicedo Ruiz, Juan Daniel
dc.contributor.advisor.none.fl_str_mv Diaztagle Fernández, Juan José
dc.contributor.author.none.fl_str_mv Caicedo Ruiz, Juan Daniel
dc.contributor.orcid.spa.fl_str_mv 0000-0001-6488-806X
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud::612 - Fisiología humana
topic 610 - Medicina y salud::612 - Fisiología humana
Respiración de la célula
Ácidos tricarboxilicos
Respiração Celular
Tricarboxylic Acids
Ciclo de krebs
Ciclo de los ácidos tricarboxílicos
Sepsis
Choque séptico
Reprogramación metabolica
Cromatografía líquida
Krebs cycle
Tricarboxylic acid cycle
Sepsis
Septic shock
Metabolic reprogramming
Liquid chromatography
dc.subject.decs.spa.fl_str_mv Respiración de la célula
Ácidos tricarboxilicos
dc.subject.decs.eng.fl_str_mv Respiração Celular
Tricarboxylic Acids
dc.subject.proposal.spa.fl_str_mv Ciclo de krebs
Ciclo de los ácidos tricarboxílicos
Sepsis
Choque séptico
Reprogramación metabolica
Cromatografía líquida
dc.subject.proposal.eng.fl_str_mv Krebs cycle
Tricarboxylic acid cycle
Sepsis
Septic shock
Metabolic reprogramming
Liquid chromatography
description ilustraciones
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-10-05T16:00:38Z
dc.date.available.none.fl_str_mv 2023-10-05T16:00:38Z
dc.date.issued.none.fl_str_mv 2023-10-02
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/84766
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/84766
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Diaztagle Fernández, Juan José6a5c3f8d549d0a68744a83a0196723e2Caicedo Ruiz, Juan Daniel8e00e258277ad9a2d92eab830a2e77fc0000-0001-6488-806X2023-10-05T16:00:38Z2023-10-05T16:00:38Z2023-10-02https://repositorio.unal.edu.co/handle/unal/84766Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustracionesRESUMEN. Introducción: El ciclo de Krebs o ciclo de los ácidos tricarboxilicos (CAT) se considera tradicionalmente como una vía cíclica que opera acoplada a la fosforilación oxidativa. En condiciones de hipoxia se asume que el CAT se encuentra detenido. Sin embargo, la persistencia del funcionamiento del CAT en estados de choque ha sido documentada, así como la “fragmentación” de sus reacciones enzimáticas en modelos animales de hipoxia conllevando a la persistencia de sus reacciones catabólicas. El objetivo de este trabajo es identificar la cinética de los intermediarios del CAT en el plasma durante el choque por endotoxemia en un modelo porcino. Métodos: Estudio experimental, a 9 porcinos se les suministró LPS de E. Coli hasta el desarrollo de choque (PAM<50 mmHg), 3 porcinos se utilizaron como controles. Se obtuvieron muestras de sangre venosa en 3 tiempos: T0: inmediatamente antes del suministro de endotoxina o placebo, T1: 3-horas posterior a su suministro, T2: 6-horas posterior a su suministro. La cuantificación de los intermediarios del CAT, lactato y piruvato en plasma se realizó mediante cromatografía liquida. Como medida de la progresión a la anaerobiosis se utilizó la relación entre lactato y piruvato (L/P). Las diferencias de las medianas de las concentraciones entre T0-T1, T0-T2 y T1-T2 para cada grupo se analizaron con el test de Wilkoxon, igualmente, se analizó la diferencia entre grupo control y grupo endotoxina para cada tiempo. Finalmente, se realizó una regresión lineal univariada entre las concentraciones de los intermediarios del CAT y los valores de lactato. Resultados: En el grupo control el citrato fue el metabolito del CAT predominante en plasma en T2 (180 μmol/L). En contraste, en condiciones de endotoxemia el succinato fue el metabolito mas abundante a nivel plasmatico (783 μmol/L). En el grupo de endotoxemia los únicos metabolitos del CAT que presentaron variaciones significativas fueron: Succinato (T0:370.00 - T1:586.67 - T2:783.33 μmol/L; p<0.05) y citrato (T0:190 - T2: 540 μmol/L; p<0.05). Los demás intermediarios no presentaron variaciones significativas durante la experimentación. La regresión lineal entre los niveles de lactato y succinato obtuvo un coeficiente de determinación de 0.347 (p=0.003). Conclusiones: La elevación reportada en los niveles de succinato y citrato durante el transcurso de nuestro biomodelo de endotoxemia sugiere un incremento de la actividad catabólica a nivel celular. Así mismo, los niveles elevados de succinato se correlacionan de manera parcial con la hiperlactatemia que se observa durante la endotoxemia. (Texto tomado de la fuente)SUMMARY. Introduction: The Krebs cycle or tricarboxylic acid cycle (TAC) is traditionally considered as a cyclic pathway that operates coupled to oxidative phosphorylation. Under conditions of hypoxia it is assumed that the TAC is arrested. However, the persistence of TAC function in shock states has been documented, as well as the "fragmentation" of its enzymatic reactions in animal models of hypoxia leading to the persistence of its catabolic reactions. The aim of this work is to identify the kinetics of TAC intermediates in plasma during endotoxemia shock in a swine model. Methods: Experimental study, 9 swine were given LPS of E. coli until the development of shock (MAP<50 mmHg), 3 swine were used as controls. Venous blood samples were obtained at 3 times: T0: immediately before endotoxin or placebo administration, T1: 3-hours after administration, T2: 6-hours after administration. The quantification of TAC intermediates, lactate and pyruvate in plasma was performed by liquid chromatography. The lactate to pyruvate ratio (L/P) was used as a measure of progression to anaerobiosis. The differences in median concentrations between T0-T1, T0-T2 and T1-T2 for each group were analyzed with the Wilkoxon test, as well as the difference between the control group and the endotoxin group for each time. Finally, a univariate linear regression was performed between the concentrations of TAC intermediates and lactate values. Results: In the control group citrate was the predominant TAC metabolite in plasma at T2 (180 μmol/L). In contrast, under endotoxemia conditions succinate was the most abundant metabolite at plasma level (783 μmol/L). In the endotoxemia group the only TAC metabolites that presented significant variations were: succinate (T0:370.00 - T1:586.67 - T2:783.33 μmol/L; p<0.05) and citrate (T0:190 - T2: 540 μmol/L; p<0.05). The other intermediates did not present significant variations during the experimentation. Linear regression between lactate and succinate levels obtained a coefficient of determination of 0.347 (p=0.003). Conclusions: The reported elevation in succinate and citrate levels during the course of our endotoxemia biomodel suggests increased catabolic activity at the cellular level. Likewise, elevated succinate levels correlate partially with the hyperlactatemia observed during endotoxemia.Maestría112 páginasapplication/pdfspa610 - Medicina y salud::612 - Fisiología humanaRespiración de la célulaÁcidos tricarboxilicosRespiração CelularTricarboxylic AcidsCiclo de krebsCiclo de los ácidos tricarboxílicosSepsisChoque sépticoReprogramación metabolicaCromatografía líquidaKrebs cycleTricarboxylic acid cycleSepsisSeptic shockMetabolic reprogrammingLiquid chromatographyAnálisis de los intermediarios del ciclo de krebs durante las primeras 6 horas de endotoxemia inducida por lipopolisacárido en un modelo de sepsis porcina.Krebs cycle intermediates during the first 6 hours of lipopolysaccharide-induced endotoxemia in a swine sepsis modelTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBogotá - Medicina - Maestría en FisiologíaFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá1. 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