Evaluación de la capacidad prebiótica del Biopolímero Bilac®
Ilustraciones a color, fotografías, tablas
- Autores:
-
Zambrano Pardo, Liliana del Pilar
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/79461
- Palabra clave:
- 570 - Biología::576 - Genética y evolución
Prebióticos
Prebiotics
Enfermedades Gastrointestinales
Gastrointestinal Diseases
Microbiota
Prebiótico
Biopolímero
Inulina
Prebiotic
Biopolymer
Inulin
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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|
dc.title.spa.fl_str_mv |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® |
dc.title.translated.eng.fl_str_mv |
Evaluation of the prebiotic capacity of the Bilac® biopolymer |
title |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® |
spellingShingle |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® 570 - Biología::576 - Genética y evolución Prebióticos Prebiotics Enfermedades Gastrointestinales Gastrointestinal Diseases Microbiota Prebiótico Biopolímero Inulina Prebiotic Biopolymer Inulin |
title_short |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® |
title_full |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® |
title_fullStr |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® |
title_full_unstemmed |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® |
title_sort |
Evaluación de la capacidad prebiótica del Biopolímero Bilac® |
dc.creator.fl_str_mv |
Zambrano Pardo, Liliana del Pilar |
dc.contributor.advisor.none.fl_str_mv |
Cobos de Rangel, Olga Patricia Meléndez Mejía, Adelina del Pilar |
dc.contributor.author.none.fl_str_mv |
Zambrano Pardo, Liliana del Pilar |
dc.contributor.researchgroup.spa.fl_str_mv |
Biopolímeros y Biofuncionales |
dc.subject.ddc.spa.fl_str_mv |
570 - Biología::576 - Genética y evolución |
topic |
570 - Biología::576 - Genética y evolución Prebióticos Prebiotics Enfermedades Gastrointestinales Gastrointestinal Diseases Microbiota Prebiótico Biopolímero Inulina Prebiotic Biopolymer Inulin |
dc.subject.decs.none.fl_str_mv |
Prebióticos Prebiotics Enfermedades Gastrointestinales Gastrointestinal Diseases Microbiota |
dc.subject.proposal.spa.fl_str_mv |
Prebiótico Biopolímero Inulina |
dc.subject.proposal.eng.fl_str_mv |
Prebiotic Biopolymer Inulin |
description |
Ilustraciones a color, fotografías, tablas |
publishDate |
2020 |
dc.date.issued.none.fl_str_mv |
2020 |
dc.date.accessioned.none.fl_str_mv |
2021-04-30T17:20:38Z |
dc.date.available.none.fl_str_mv |
2021-04-30T17:20:38Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
status_str |
acceptedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/79461 |
dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
url |
https://repositorio.unal.edu.co/handle/unal/79461 https://repositorio.unal.edu.co/ |
identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.references.spa.fl_str_mv |
Abásolo, P. (2014). Optimización de un método de HPLC con detección mediante Índice de refracción para la cuantificación de carbohidratos solubles en forrajes (Magister). Universidad de Oviedo. Başaran, U., Akkbik, M., Mut, H., Gülümser, E., Çopur Doğrusöz, M., Koçoğlu, S., (2017). High-performance liquid chromatography with refractive index detection for the determination of inulin in chicory roots. Anal. Lett. 51, 83–95. Bernal Castro, c. (2017). Evaluación del comportamiento de cultivos probióticos y prebióticos en bebidas de frutos rojos (Magister). Universidad Nacional de Colombia. Bruno F. A.; Lankaputhra W. E. V. y Shan N. P. (2002). Growth, Viability and Activity of Bifidobacterium spp. In Skin Milk Containig Prebiotics. Journal of Food Science. 67:2740-44. Carvalho A.L., Helmolz, K., Nodet, C., Molzer, C., Leonard, C., McKevith, B., Thielecke, F., Jackson, K., Tuohy, K. (2010). Determination of the in vivo prebiotic potential of a maize-based whole grain breakfast cereal: a human feeding study. British Journal of Nutrition. Castañeda, G. (2017). Microbiota intestinal, probióticos y prebióticos. Enferm Inv (Ambato).; 2(4):156-160. Enfermería Investiga, Investigación, Vinculación, Docencia y Gestión. Vol. 2 No 4. (Oct-Dic). Chung D. H and Day D. F. (2002). Glucooligosacarides from Leuconostoc mesenteroides B – 742 (ATCC 13146): A potencial prebiotic. Journal of Industrial microbiology and Biotechnology. 29: 196 – 199. Costa, M., Frasao, B., Lima, B., Rodrigues, B., & Junior, C. (2016). Simultaneous analysis of carbohydrates and organic acids by HPLC-DAD-RI for monitoring goats milk yogurts fermentation. Talanta, 152, 162-170. doi: 10.1016/j.talanta.2016.01.061 Doran, P. (1995). Principios de Ingeniería de los Bioprocesos, Editorial Acribia. S.A. España. Drakoularakou, A., McCartney, A. L., Rastall, R. A., & Gibson, G. R. (2004). Established and emerging prebiotics and their effects on the gut microflora. Agro Food Industry Hi-Tech, 15, 18-20. Drzikova B, Dongowski G, Gebhardt E, Habel A. The composition of dietary fibre-rich extrudates from oat affects bile acid binding and fermentation in vitro. Food Chemistry. 2005; (90):181-92. El-Kholy, W., Aamer, R., & Ali, A. (2020). Utilization of inulin extracted from chicory (Cichorium intybus L.) roots to improve the properties of low-fat synbiotic yoghurt. Annals of Agricultural Sciences, 65(1), 59-67. doi: 10.1016/j.aoas.2020.02.002 Ficha técnica biopolímero Bilac®. Consulta: 2010. Figueroa, I., Rodríguez, G., Hernández, H., Jiménez, J., Lorena, G., García, M., & Cruz, A. (2009). Efecto de prebióticos en el crecimiento de probióticos. In XIII Congreso Nacional de Biotecnología y Bioingeniería (p. 1). México D.F: Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Fuller, R. & Gibson, G. R. (1997). Modification of the intestinal microflora using probiotics and prebiotics. Scandinavian Journal of Gastroenterology, Supplement, 32, 28-31. Fuller, R. & Gibson, G. R. (1998). Probiotics and prebiotics: Microflora management for improved gut health. Clinical Microbiology and Infection, 4, 477-480. García Albornoz, m. (2006). Determinación y caracterización de fructanos provenientes de henequén (Magister). Centro de investigación científica de yucatán , a.c. Gibson, G. R. (2004). Fibre and effects on probiotics (the prebiotic concept). Clinical Nutrition, Supplement, 1, 25-31. Gibson, G. R. and Roberfroid, M. (1995). Dietary Modulation of the Human Colonie Microbiota: Introducing the Concept of Prebiotics. The Journal of Nutrition. 125: 1401-1412. Gibson, G. R. and Roberfroid, M. (2008). Handbook of prebiotics. CRC Press. Boca Raton. 485 p. Gibson, G. R., Probert, H. M., Van Loo, J., Rastall, R. A., & Roberfroid, M. B. (2004). Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics. Nutrition Research Reviews, 17,259-275. Gomez, E., Tuohy, K. M., Gibson, G. R., Klinder, A., & Costabile, A. (2010). In vitro evaluation of the fermentation properties and potential prebiotic activity of Agave fructans. Journal of Applied Microbiology, 108, 2114-2121. González, B., Gómez, M., Jiménez, Z. (2003). Bacteriocinas de Probióticos. Revista Salud Pública y Nutrición. Vol. 4 No. 2. Abril - Junio. Facultad de Salud Pública y Nutrición (Universidad Autónoma de Nuevo León). Grimoud J, Durand H, Courtin C, Monsan P, Ouarné F, Theodorou V, Roques C. (2010). In vitro screening of probiotic lactic acid bacteria and prebiotic glucooligosaccharides to select effective synbiotics. Anaerobe. 16: 493-500. Guarner, F., Sanders, M., Eliakim R., Fedorak, R., Gangl, A., Garisch, J., Kaufmann, P., Karakan, T., Khan, A., Kim, N., De Paula, J., Ramakrishna, B., Shanahan, F., Szajewska, H., Thomson, A., Le Mair, A. Merenstein, D., Salminen, S. (2017). Probióticos y prebióticos. Guías Mundiales de la Organización Mundial de Gastroenterología. Guía mundial de la WGO. World Gastroenterology Organisation. Herawati, E., Miftakhussolikhah, M., Nurhayati, R., Sari, K., & Pranoto, Y. (2019). Oligosaccharides Profile and Prebiotic Potential of Gembolo Tuber (Dioscorea bulbifera). IOP Conference Series: Earth And Environmental Science, 251, 012048. doi: 10.1088/1755-1315/251/1/012048 ISSAP, (2008). International Scientific Asociation for probiotics and prebiotics (6th meeting in Ontario, Canada, November. Khangwal, I., & Shukla, P. (2019). Potential prebiotics and their transmission mechanisms: Recent approaches. Journal Of Food And Drug Analysis, 27(3), 649-656. doi: 10.1016/j.jfda.2019.02.003 Kolida S. and Gibson, G. R. (2007). Prebiotic Capacity of Inulin-Type Fructans. The Journal of Nutrition. Suplement. 2503S – 2506S. Kun Lee, Y., and Salminem, S. (2009). Handbook of probiotics and prebiotics. 2 edition. New Jersey. 595 p. Matissek, R., Schnepel, F., Steiner, G., (1992). Análisis de los Alimentos, Fundamentos, Métodos, Aplicaciones, Editorial Acribia. S.A. España. McLaughlin, H., Motherway, M., Lakshminarayanan, B., Stanton, C., Paul Ross, R., & Brulc, J. et al. (2015). Carbohydrate catabolic diversity of Bifidobacteria and Lactobacilli of human origin. International Journal of Food Microbiology, 203, 109-121. doi: 10.1016/j.ijfoodmicro.2015.03.008 Ministerios de Agricultura, Industria del yogur sigue creciendo en Colombia, Agronet-MinAgricultura, Bogotá, 2018. Mitsou E, Panopoulou N, Turunen K, Spiliotis V, Kyriacou A. (2010). Prebiotic potential of barley derived b-glucan at low intake levels: A randomised, double-blinded, placebo-controlled clinical study. Food Research international 43: 1086 -1092. Muñoz Mabel, 2011. Efecto de los fructooligosacaridos en la producción de bacteriocinas por aislados nativos de lactobacillus spp. Tesis de maestría en Ciencia y tecnología de alimentos. Neysens P, Messens W, Gevers, D, Swings J, De Vuyst, L. 2003. Biphasic Kinetics of growth and bacteriocin production with Lactobacillus Amylovorus DCE 471 occur under stress conditions. Microbiology. 149, 1073 – 1082. Olveira G y González I. 2007 Probióticos y prebióticos en la práctica clínica. Nutr Hosp.;22(Supl. 2):26-34. Unidad de Nutrición Clínica y Dietética. Servicio de Endocrinología y Nutrición. Hospital Regional Universitario Carlos Haya. Málaga. Instituto de Salud Carlos III. Red RD06/001510008. España. ISSN 0212-1611 Ortega, R., Marcos, A., Aranceta J., Mateos J., Requejo, A., Serra, L. (2002). Alimentos Funcionales. Probioticos. Editorial Médica Panamericana. Madrid. España. Pedone C. A, Bernabeu A. O, Postaire E. R, Bouley C. F, Reinert P. (1999). The effect of suplementation with milk fermented by Lactobacullus casei (strain DN- 114 001) on acute diarrhoea in children attending day care centres. Int J Clin Pract. 53: 179 -184 Pizarro C, Sebastián, Ronco M, Ana María, & Gotteland R, Martín. (2014). ß-glucanos: ¿qué tipos existen y cuáles son sus beneficios en la salud?ß-glucans: what types exist and what are their health benefits?. Revista chilena de nutrición, 41(4), 439-446. https://dx.doi.org/10.4067/S0717-75182014000400014 Prescott, L. (2005). Microbiología. McGraw Hill. Quattrocchi, O., Abelaira de Andrizzi, S., Laba, R. (1992) Introducción a la HPLC, Aplicación y Practica, Artes gráficas Farro SA, Argentina. Rastall, R. (2013). Gluco and galacto-oligosaccharides in food. Current Opinion In Clinical Nutrition And Metabolic Care, 16(6), 675-678. doi: 10.1097/mco.0b013e328365b5fd Roberfroid M, Gibson GR, Hoyles L, McCartney AL, Rastall R, Rowland I, Wolvers D, Watzl B, Szajewska H, Stahl B, Guarner F, Respondek F, Whelan K, Coxam V, Davicco MJ, Léotoing L, Wittrant Y, Delzenne NM, Cani PD, Neyrinck AM, Meheust A. (2010). Prebiotic effects: metabolic and health benefits. Br J Nutr. Aug;104 Suppl 2:S1-63. Roberfroid, M; Van Loo, J; Gibson, G. 1998. The bifidogenic nature of chicory inulin and its hidrolysis products. The journal of nutrition. 128: 11-19. Saavedra J. M, Bauman N. A, Oung I, Perman J.A. Yolken R.H. 1994. Feeding of Bifidobacterium bifidum and Streptococcus termofilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. Lancet. 334: 1046 -1049. Salminen, S., Bouley, C., Boutron-Ruault, M. C., Cummings, J. H., Franck, A., Gibson, G. R. et al. (1998). Functional food science and gastrointestinal physiology and function. British Journal of Nutrition, 80, S147-S171. Sarbini, S., Kolida, S., Gibson, G., & Rastall, R. (2012). In vitrofermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects. British Journal Of Nutrition, 109(11), 1980-1989. doi: 10.1017/s0007114512004205 Saulnier, D. M., Kolida, S., & Gibson, G. R. (2009). Microbiology of the human intestinal tract and approaches for its dietary modulation. Current Pharmaceutical Design, 15, 1403-1414. Schaafsma, G., & Slavin, J. (2014). Significance of Inulin Fructans in the Human Diet. Comprehensive Reviews In Food Science And Food Safety, 14(1), 37-47. doi: 10.1111/1541-4337.12119 Schell, M. A., Karmirantzou, M., Snel, B., Vilanova, D., Berger, B., Pessi, G., Zwahlen, M.C. (2002). The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc. Nat. Acad. USA 99:14422–14427. Sherman P.N, Cabana, M, Gibson, G, R. Koletzko, B, V., Neu, J, Wauters, G, V., Ekhard E. Z, and Walker, W, A. (2009) Potential Roles and Clinical Utility of Prebiotics in Newborns, Infants, and Children: Proceedings from a Global Prebiotic Summit Meeting, New York City, June 27-28, 2008. J Pediatr. 155:S61-70. Shin H. S; Lee J. H; y Pestka J. (2000). Growth and Viability of Comercial Bifidobacterium spp. in Skim Milk Containing Oligosaccharides and Inulin. Journal of Food Science. 65:884-87. Sims, I., Ryan, J., & Kim, S. (2014). In vitro fermentation of prebiotic oligosaccharides by Bifidobacterium lactis HN019 and Lactobacillus spp. Anaerobe, 25, 11-17. doi: 10.1016/j.anaerobe.2013.11.001 Sungsoo S., and Finochiaro T. (2010). Handbook of probiotics and prebiotics ingredients: health benefits and food applications. CRC Press. Boca Raton. 435 p. Tingirikari, J., Gomes, W., & Rodrigues, S. (2017). Efficient Production of Prebiotic Gluco-oligosaccharides in Orange Juice Using Immobilized and Co-immobilized Dextransucrase. Applied Biochemistry And Biotechnology, 183(4), 1265-1281. doi: 10.1007/s12010-017-2496-2 Tuohy K. M, Robert, H. M, Smelkal, C. W, Gibson G. R. (2003). Using probiotics and prebiotics to improve gut health. Therapeutic focus. 8. 15: 692 – 700. Vanderhoff, J. A, Whitney, D.B, Antonson D. L, Hanner T. L, Lupo J. V., Young, R. J. (1999). Lactobacillus GG in the prevention of antibiotic – associated diarrehea in children J Pediatr. 135: 564 – 568. Vives, María Carolina. (2010). Fermentabilidad in vivo del bipolímero Bilac® y determinación de la producción de ácidos grasos de cadena corta. Trabajo de grado de Especialización en Ciencia y Tecnología de Alimentos. Universidad Nacional de Colombia. Bogotá. Wang, Y. (2009). Prebiotics: Present and future in food science and technology. Food Research International 42: 8–12. Watson, D., OConnell Motherway, M., Schoterman, M., van Neerven, R., Nauta, A., & van Sinderen, D. (2013). Selective carbohydrate utilization by Lactobacilli and Bifidobacteria. Journal of Applied Microbiology, 114(4), 1132-1146. doi: 10.1111/jam.12105 WHO. (2011). Organización mundial de la salud. Probióticos en la alimentación. Disponible en:http://www.who.int/foodsafety/publications/fs_management/probiotics2/en. Zeaiter, Z., Regonesi, M., Cavini, S., Labra, M., Sello, G., & Di Gennaro, P. (2019). Extraction and Characterization of Inulin-Type Fructans from Artichoke Wastes and Their Effect on the Growth of Intestinal Bacteria Associated with Health. Biomed Research International, 2019, 1-8. doi: 10.1155/2019/1083952 Ziemer, C. J. & Gibson, G. R. (1998). An overview of probiotics, prebiotics and synbiotics in the functional food concept: Perspectives and future strategies. International Dairy Journal, 8, 473-479. |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidad Nacional de Colombia |
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Bogotá - Ciencias - Maestría en Ciencias - Microbiología |
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Facultad de Ciencias |
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Bogotá |
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Universidad Nacional de Colombia - Sede Bogotá |
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Universidad Nacional de Colombia |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cobos de Rangel, Olga Patricia903e98f44a05b1823f4675b590cac5a2Meléndez Mejía, Adelina del Pilarb9cb1ce59dc3e1d30eedf3d4668f56f7Zambrano Pardo, Liliana del Pilar180cf41d644c762d48fa85480a607fe3Biopolímeros y Biofuncionales2021-04-30T17:20:38Z2021-04-30T17:20:38Z2020https://repositorio.unal.edu.co/handle/unal/79461Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones a color, fotografías, tablasEn el presente estudio se buscó evaluar la capacidad prebiótica del biopolímero Bilac® en condiciones in vitro, determinando la capacidad de algunas bacterias probióticas, como Bifidobacterium y Lactobacillus, de utilizar este biopolímero como fuente de carbono. Se comprobó que en un uso conjunto entre probióticos y prebióticos es posible que la viabilidad de estas bacterias se mantenga alta en función del tiempo, para su uso en bebidas y alimentos. Además, el estudio contempló un componente in vivo, en el cual se realizó un estudio clínico en humanos en donde se proporcionó en la dieta de las personas dosis diarias del biopolímero. Los resultados de estos ensayos permitieron concluir que, al consumir este biopolímero en galletas y una bebida láctea, aumenta la microbiota intestinal benéfica, y mantiene o disminuye levemente los coliformes.Certain chronic non communicable diseases, and gastrointestinal diseases can be prevented and treated with the consumption of functional foods such as probiotics and prebiotics. Previous studies both in vitro and in vivo in animals and humans show the beneficial effect that gives the guest the daily consumption of these foods. Prebiotics include selectively fermentable soluble fibers by the colonic microbiota, prevents intestinal discomfort such as constipation, so its recommended consumption. In Colombia certain types of diarrhea and gastrointestinal diseases in children are cause of mortality. These diseases also affect adults and the elderly, as significant changes occur in the intestinal flora towards a potentially harmful composition. Thus, we have increased the number of studies on prebiotics and probiotics to evaluate the modification of the colonic microbiota towards a favorable this beneficial physiological effects on the host composition. The Bilac® biopolymer obtained at the Institute of Biotechnology of the National University of Colombia presents important for use as prebiotic properties, as it is high in soluble fiber, it is an alpha - glucan, α 2-6 linked a glucooligosaccharide. It has been previously reported to have prebiotic effects glucoligosaccharide because they are non-digestible carbohydrates in the upper gastrointestinal tract, reaching the colon intact where they are fermented by beneficial intestinal microbiota. In the present study aimed to evaluate the ability prebiotic Bilac® biopolymer under in vitro by determining the capacity of some probiotic bacteria, such as Bifidobacterium and Lactobacillus, using this biopolymer as a source of carbon. It was found that in a joint between probiotics and prebiotics use it is possible that the viability of the bacteria is kept high in terms of time, for use in beverages and foods. In addition, the study included an in vivo component, in which a clinical study in humans in which it was provided in the diet of people daily dose of the biopolymer. The results of these tests led to the conclusion that this biopolymer consuming cookies and milk drink, increases beneficial intestinal microbiota, and maintained or slightly decreased coliforms.MaestríaBioprocesos y Bioprospección1 recurso en línea (153 páginas)application/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotáUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::576 - Genética y evoluciónPrebióticosPrebioticsEnfermedades GastrointestinalesGastrointestinal DiseasesMicrobiotaPrebióticoBiopolímeroInulinaPrebioticBiopolymerInulinEvaluación de la capacidad prebiótica del Biopolímero Bilac®Evaluation of the prebiotic capacity of the Bilac® biopolymerTrabajo de grado - Maestríainfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbásolo, P. (2014). Optimización de un método de HPLC con detección mediante Índice de refracción para la cuantificación de carbohidratos solubles en forrajes (Magister). Universidad de Oviedo.Başaran, U., Akkbik, M., Mut, H., Gülümser, E., Çopur Doğrusöz, M., Koçoğlu, S., (2017). High-performance liquid chromatography with refractive index detection for the determination of inulin in chicory roots. Anal. Lett. 51, 83–95.Bernal Castro, c. (2017). Evaluación del comportamiento de cultivos probióticos y prebióticos en bebidas de frutos rojos (Magister). Universidad Nacional de Colombia.Bruno F. A.; Lankaputhra W. E. V. y Shan N. P. (2002). Growth, Viability and Activity of Bifidobacterium spp. In Skin Milk Containig Prebiotics. Journal of Food Science. 67:2740-44.Carvalho A.L., Helmolz, K., Nodet, C., Molzer, C., Leonard, C., McKevith, B., Thielecke, F., Jackson, K., Tuohy, K. (2010). Determination of the in vivo prebiotic potential of a maize-based whole grain breakfast cereal: a human feeding study. British Journal of Nutrition.Castañeda, G. (2017). Microbiota intestinal, probióticos y prebióticos. Enferm Inv (Ambato).; 2(4):156-160. Enfermería Investiga, Investigación, Vinculación, Docencia y Gestión. Vol. 2 No 4. (Oct-Dic).Chung D. H and Day D. F. (2002). Glucooligosacarides from Leuconostoc mesenteroides B – 742 (ATCC 13146): A potencial prebiotic. Journal of Industrial microbiology and Biotechnology. 29: 196 – 199.Costa, M., Frasao, B., Lima, B., Rodrigues, B., & Junior, C. (2016). Simultaneous analysis of carbohydrates and organic acids by HPLC-DAD-RI for monitoring goats milk yogurts fermentation. Talanta, 152, 162-170. doi: 10.1016/j.talanta.2016.01.061Doran, P. (1995). Principios de Ingeniería de los Bioprocesos, Editorial Acribia. S.A. España. Drakoularakou, A., McCartney, A. L., Rastall, R. A., & Gibson, G. R. (2004). Established and emerging prebiotics and their effects on the gut microflora. Agro Food Industry Hi-Tech, 15, 18-20.Drzikova B, Dongowski G, Gebhardt E, Habel A. The composition of dietary fibre-rich extrudates from oat affects bile acid binding and fermentation in vitro. Food Chemistry. 2005; (90):181-92.El-Kholy, W., Aamer, R., & Ali, A. (2020). Utilization of inulin extracted from chicory (Cichorium intybus L.) roots to improve the properties of low-fat synbiotic yoghurt. Annals of Agricultural Sciences, 65(1), 59-67. doi: 10.1016/j.aoas.2020.02.002Ficha técnica biopolímero Bilac®. Consulta: 2010.Figueroa, I., Rodríguez, G., Hernández, H., Jiménez, J., Lorena, G., García, M., & Cruz, A. (2009). Efecto de prebióticos en el crecimiento de probióticos. In XIII Congreso Nacional de Biotecnología y Bioingeniería (p. 1). México D.F: Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa.Fuller, R. & Gibson, G. R. (1997). Modification of the intestinal microflora using probiotics and prebiotics. Scandinavian Journal of Gastroenterology, Supplement, 32, 28-31.Fuller, R. & Gibson, G. R. (1998). Probiotics and prebiotics: Microflora management for improved gut health. Clinical Microbiology and Infection, 4, 477-480.García Albornoz, m. (2006). Determinación y caracterización de fructanos provenientes de henequén (Magister). Centro de investigación científica de yucatán , a.c.Gibson, G. R. (2004). Fibre and effects on probiotics (the prebiotic concept). Clinical Nutrition, Supplement, 1, 25-31.Gibson, G. R. and Roberfroid, M. (1995). Dietary Modulation of the Human Colonie Microbiota: Introducing the Concept of Prebiotics. The Journal of Nutrition. 125: 1401-1412.Gibson, G. R. and Roberfroid, M. (2008). Handbook of prebiotics. CRC Press. Boca Raton. 485 p.Gibson, G. R., Probert, H. M., Van Loo, J., Rastall, R. A., & Roberfroid, M. B. (2004). Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics. Nutrition Research Reviews, 17,259-275.Gomez, E., Tuohy, K. M., Gibson, G. R., Klinder, A., & Costabile, A. (2010). In vitro evaluation of the fermentation properties and potential prebiotic activity of Agave fructans. Journal of Applied Microbiology, 108, 2114-2121.González, B., Gómez, M., Jiménez, Z. (2003). Bacteriocinas de Probióticos. Revista Salud Pública y Nutrición. Vol. 4 No. 2. Abril - Junio. Facultad de Salud Pública y Nutrición (Universidad Autónoma de Nuevo León).Grimoud J, Durand H, Courtin C, Monsan P, Ouarné F, Theodorou V, Roques C. (2010). In vitro screening of probiotic lactic acid bacteria and prebiotic glucooligosaccharides to select effective synbiotics. Anaerobe. 16: 493-500.Guarner, F., Sanders, M., Eliakim R., Fedorak, R., Gangl, A., Garisch, J., Kaufmann, P., Karakan, T., Khan, A., Kim, N., De Paula, J., Ramakrishna, B., Shanahan, F., Szajewska, H., Thomson, A., Le Mair, A. Merenstein, D., Salminen, S. (2017). Probióticos y prebióticos. Guías Mundiales de la Organización Mundial de Gastroenterología. Guía mundial de la WGO. World Gastroenterology Organisation.Herawati, E., Miftakhussolikhah, M., Nurhayati, R., Sari, K., & Pranoto, Y. (2019). Oligosaccharides Profile and Prebiotic Potential of Gembolo Tuber (Dioscorea bulbifera). IOP Conference Series: Earth And Environmental Science, 251, 012048. doi: 10.1088/1755-1315/251/1/012048ISSAP, (2008). International Scientific Asociation for probiotics and prebiotics (6th meeting in Ontario, Canada, November.Khangwal, I., & Shukla, P. (2019). Potential prebiotics and their transmission mechanisms: Recent approaches. Journal Of Food And Drug Analysis, 27(3), 649-656. doi: 10.1016/j.jfda.2019.02.003Kolida S. and Gibson, G. R. (2007). Prebiotic Capacity of Inulin-Type Fructans. The Journal of Nutrition. Suplement. 2503S – 2506S.Kun Lee, Y., and Salminem, S. (2009). Handbook of probiotics and prebiotics. 2 edition. New Jersey. 595 p.Matissek, R., Schnepel, F., Steiner, G., (1992). Análisis de los Alimentos, Fundamentos, Métodos, Aplicaciones, Editorial Acribia. S.A. España.McLaughlin, H., Motherway, M., Lakshminarayanan, B., Stanton, C., Paul Ross, R., & Brulc, J. et al. (2015). Carbohydrate catabolic diversity of Bifidobacteria and Lactobacilli of human origin. International Journal of Food Microbiology, 203, 109-121. doi: 10.1016/j.ijfoodmicro.2015.03.008Ministerios de Agricultura, Industria del yogur sigue creciendo en Colombia, Agronet-MinAgricultura, Bogotá, 2018.Mitsou E, Panopoulou N, Turunen K, Spiliotis V, Kyriacou A. (2010). Prebiotic potential of barley derived b-glucan at low intake levels: A randomised, double-blinded, placebo-controlled clinical study. Food Research international 43: 1086 -1092.Muñoz Mabel, 2011. Efecto de los fructooligosacaridos en la producción de bacteriocinas por aislados nativos de lactobacillus spp. Tesis de maestría en Ciencia y tecnología de alimentos.Neysens P, Messens W, Gevers, D, Swings J, De Vuyst, L. 2003. Biphasic Kinetics of growth and bacteriocin production with Lactobacillus Amylovorus DCE 471 occur under stress conditions. Microbiology. 149, 1073 – 1082.Olveira G y González I. 2007 Probióticos y prebióticos en la práctica clínica. Nutr Hosp.;22(Supl. 2):26-34. Unidad de Nutrición Clínica y Dietética. Servicio de Endocrinología y Nutrición. Hospital Regional Universitario Carlos Haya. Málaga. Instituto de Salud Carlos III. Red RD06/001510008. España. ISSN 0212-1611Ortega, R., Marcos, A., Aranceta J., Mateos J., Requejo, A., Serra, L. (2002). Alimentos Funcionales. Probioticos. Editorial Médica Panamericana. Madrid. España.Pedone C. A, Bernabeu A. O, Postaire E. R, Bouley C. F, Reinert P. (1999). The effect of suplementation with milk fermented by Lactobacullus casei (strain DN- 114 001) on acute diarrhoea in children attending day care centres. Int J Clin Pract. 53: 179 -184Pizarro C, Sebastián, Ronco M, Ana María, & Gotteland R, Martín. (2014). ß-glucanos: ¿qué tipos existen y cuáles son sus beneficios en la salud?ß-glucans: what types exist and what are their health benefits?. Revista chilena de nutrición, 41(4), 439-446. https://dx.doi.org/10.4067/S0717-75182014000400014Prescott, L. (2005). Microbiología. McGraw Hill.Quattrocchi, O., Abelaira de Andrizzi, S., Laba, R. (1992) Introducción a la HPLC, Aplicación y Practica, Artes gráficas Farro SA, Argentina.Rastall, R. (2013). Gluco and galacto-oligosaccharides in food. Current Opinion In Clinical Nutrition And Metabolic Care, 16(6), 675-678. doi: 10.1097/mco.0b013e328365b5fdRoberfroid M, Gibson GR, Hoyles L, McCartney AL, Rastall R, Rowland I, Wolvers D, Watzl B, Szajewska H, Stahl B, Guarner F, Respondek F, Whelan K, Coxam V, Davicco MJ, Léotoing L, Wittrant Y, Delzenne NM, Cani PD, Neyrinck AM, Meheust A. (2010). Prebiotic effects: metabolic and health benefits. Br J Nutr. Aug;104 Suppl 2:S1-63.Roberfroid, M; Van Loo, J; Gibson, G. 1998. The bifidogenic nature of chicory inulin and its hidrolysis products. The journal of nutrition. 128: 11-19.Saavedra J. M, Bauman N. A, Oung I, Perman J.A. Yolken R.H. 1994. Feeding of Bifidobacterium bifidum and Streptococcus termofilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. Lancet. 334: 1046 -1049.Salminen, S., Bouley, C., Boutron-Ruault, M. C., Cummings, J. H., Franck, A., Gibson, G. R. et al. (1998). Functional food science and gastrointestinal physiology and function. British Journal of Nutrition, 80, S147-S171.Sarbini, S., Kolida, S., Gibson, G., & Rastall, R. (2012). In vitrofermentation of commercial α-gluco-oligosaccharide by faecal microbiota from lean and obese human subjects. British Journal Of Nutrition, 109(11), 1980-1989. doi: 10.1017/s0007114512004205Saulnier, D. M., Kolida, S., & Gibson, G. R. (2009). Microbiology of the human intestinal tract and approaches for its dietary modulation. Current Pharmaceutical Design, 15, 1403-1414.Schaafsma, G., & Slavin, J. (2014). Significance of Inulin Fructans in the Human Diet. Comprehensive Reviews In Food Science And Food Safety, 14(1), 37-47. doi: 10.1111/1541-4337.12119Schell, M. A., Karmirantzou, M., Snel, B., Vilanova, D., Berger, B., Pessi, G., Zwahlen, M.C. (2002). The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc. Nat. Acad. USA 99:14422–14427.Sherman P.N, Cabana, M, Gibson, G, R. Koletzko, B, V., Neu, J, Wauters, G, V., Ekhard E. Z, and Walker, W, A. (2009) Potential Roles and Clinical Utility of Prebiotics in Newborns, Infants, and Children: Proceedings from a Global Prebiotic Summit Meeting, New York City, June 27-28, 2008. J Pediatr. 155:S61-70.Shin H. S; Lee J. H; y Pestka J. (2000). Growth and Viability of Comercial Bifidobacterium spp. in Skim Milk Containing Oligosaccharides and Inulin. Journal of Food Science. 65:884-87.Sims, I., Ryan, J., & Kim, S. (2014). In vitro fermentation of prebiotic oligosaccharides by Bifidobacterium lactis HN019 and Lactobacillus spp. Anaerobe, 25, 11-17. doi: 10.1016/j.anaerobe.2013.11.001Sungsoo S., and Finochiaro T. (2010). Handbook of probiotics and prebiotics ingredients: health benefits and food applications. CRC Press. Boca Raton. 435 p.Tingirikari, J., Gomes, W., & Rodrigues, S. (2017). Efficient Production of Prebiotic Gluco-oligosaccharides in Orange Juice Using Immobilized and Co-immobilized Dextransucrase. Applied Biochemistry And Biotechnology, 183(4), 1265-1281. doi: 10.1007/s12010-017-2496-2Tuohy K. M, Robert, H. M, Smelkal, C. W, Gibson G. R. (2003). Using probiotics and prebiotics to improve gut health. Therapeutic focus. 8. 15: 692 – 700.Vanderhoff, J. A, Whitney, D.B, Antonson D. L, Hanner T. L, Lupo J. V., Young, R. J. (1999). Lactobacillus GG in the prevention of antibiotic – associated diarrehea in children J Pediatr. 135: 564 – 568.Vives, María Carolina. (2010). Fermentabilidad in vivo del bipolímero Bilac® y determinación de la producción de ácidos grasos de cadena corta. Trabajo de grado de Especialización en Ciencia y Tecnología de Alimentos. Universidad Nacional de Colombia. Bogotá.Wang, Y. (2009). Prebiotics: Present and future in food science and technology. Food Research International 42: 8–12.Watson, D., OConnell Motherway, M., Schoterman, M., van Neerven, R., Nauta, A., & van Sinderen, D. (2013). Selective carbohydrate utilization by Lactobacilli and Bifidobacteria. Journal of Applied Microbiology, 114(4), 1132-1146. doi: 10.1111/jam.12105WHO. (2011). Organización mundial de la salud. Probióticos en la alimentación. Disponible en:http://www.who.int/foodsafety/publications/fs_management/probiotics2/en.Zeaiter, Z., Regonesi, M., Cavini, S., Labra, M., Sello, G., & Di Gennaro, P. (2019). Extraction and Characterization of Inulin-Type Fructans from Artichoke Wastes and Their Effect on the Growth of Intestinal Bacteria Associated with Health. Biomed Research International, 2019, 1-8. doi: 10.1155/2019/1083952Ziemer, C. J. & Gibson, G. R. (1998). An overview of probiotics, prebiotics and synbiotics in the functional food concept: Perspectives and future strategies. 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