Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+

This article describes the methodology of the validation about the identification of total mercury in blood samples by Zeeman atomic absorption spectrophotometry RA-915+ with the module of Pyrolysis PYRO-915+, suitable for the direct determination of total mercury concentrations in various matrices,...

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Autores:
Ramírez, Carolina
Olarte, Ermel
Tellez, Eliana
Palma, Marien
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/50024
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/50024
http://bdigital.unal.edu.co/43552/
Palabra clave:
mercurio
validación de métodos
espectrofotometría de absorción atómica
mercury
analytical methods validation
atomic absorption spectrophotometry
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_328b646efb97cd29704df725e796049a
oai_identifier_str oai:repositorio.unal.edu.co:unal/50024
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
title Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
spellingShingle Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
mercurio
validación de métodos
espectrofotometría de absorción atómica
mercury
analytical methods validation
atomic absorption spectrophotometry
title_short Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
title_full Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
title_fullStr Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
title_full_unstemmed Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
title_sort Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+
dc.creator.fl_str_mv Ramírez, Carolina
Olarte, Ermel
Tellez, Eliana
Palma, Marien
dc.contributor.author.spa.fl_str_mv Ramírez, Carolina
Olarte, Ermel
Tellez, Eliana
Palma, Marien
dc.subject.proposal.spa.fl_str_mv mercurio
validación de métodos
espectrofotometría de absorción atómica
mercury
analytical methods validation
atomic absorption spectrophotometry
topic mercurio
validación de métodos
espectrofotometría de absorción atómica
mercury
analytical methods validation
atomic absorption spectrophotometry
description This article describes the methodology of the validation about the identification of total mercury in blood samples by Zeeman atomic absorption spectrophotometry RA-915+ with the module of Pyrolysis PYRO-915+, suitable for the direct determination of total mercury concentrations in various matrices, that unlike other atomization techniques (graphite furnace, cold vapor or hydride generator) does not require a sample pre-treatment. With the application of this validation, is provided the reliability in the results. Most of the validation parameters were subjected to analysis of variance ANOVA with a confidence level of 95%. It was obtained a detection limit of 2.16 µg/L, a quantitation limit of 5.54µg/L and linearity in the range of 5.54 µg/L to , in addition, the precision and accuracy was evaluated, obtaining favorable results in the analysis. The calculation of the expanded uncertainty for this method was 0.610 µg/L.
publishDate 2013
dc.date.issued.spa.fl_str_mv 2013
dc.date.accessioned.spa.fl_str_mv 2019-06-29T09:37:59Z
dc.date.available.spa.fl_str_mv 2019-06-29T09:37:59Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.type.content.spa.fl_str_mv Text
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format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/50024
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/43552/
url https://repositorio.unal.edu.co/handle/unal/50024
http://bdigital.unal.edu.co/43552/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/rcolquim/article/view/45909
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Química
Revista Colombiana de Química
dc.relation.ispartofseries.none.fl_str_mv Revista Colombiana de Química; Vol. 42, núm. 3 (2013): Versión preliminar; 326-343 2357-3791 0120-2804
dc.relation.references.spa.fl_str_mv Ramírez, Carolina and Olarte, Ermel and Tellez, Eliana and Palma, Marien (2013) Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+. Revista Colombiana de Química; Vol. 42, núm. 3 (2013): Versión preliminar; 326-343 2357-3791 0120-2804 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ramírez, Carolina0186cbd0-620b-415b-a672-fa0918519f6c300Olarte, Ermel2197594f-cb01-47cf-a4fa-34fe4302a16a300Tellez, Elianadbb15bab-9c64-4634-9c66-f60c71040a48300Palma, Marien3c3e9d67-f4ff-47de-99a5-e6b5796512d13002019-06-29T09:37:59Z2019-06-29T09:37:59Z2013https://repositorio.unal.edu.co/handle/unal/50024http://bdigital.unal.edu.co/43552/This article describes the methodology of the validation about the identification of total mercury in blood samples by Zeeman atomic absorption spectrophotometry RA-915+ with the module of Pyrolysis PYRO-915+, suitable for the direct determination of total mercury concentrations in various matrices, that unlike other atomization techniques (graphite furnace, cold vapor or hydride generator) does not require a sample pre-treatment. With the application of this validation, is provided the reliability in the results. Most of the validation parameters were subjected to analysis of variance ANOVA with a confidence level of 95%. It was obtained a detection limit of 2.16 µg/L, a quantitation limit of 5.54µg/L and linearity in the range of 5.54 µg/L to , in addition, the precision and accuracy was evaluated, obtaining favorable results in the analysis. The calculation of the expanded uncertainty for this method was 0.610 µg/L.Este artículo describe la metodología de validación para la técnica de identificación de mercurio total en muestras de sangre por espectrofotometría de absorción atómica Zeeman RA-915+ con el módulo de Pirólisis PYRO-915+, adecuada para la determinación directa de las concentraciones de mercurio total en varias matrices, que a diferencia de otras técnicas de atomización (horno de grafito, vapor frio, o generador de hidruros) no necesita un pre-tratamiento de la muestra. Con la aplicación de ésta validación en la realización de los análisis se brinda la confiabilidad necesaria en la obtención y emisión de los resultados. La mayoría de parámetros de la validación, se sometieron a análisis de varianza tipo ANOVA con un nivel de confianza del 95 %. Se obtuvo un límite de detección de 2.16 µg/L, un límite de cuantificación de 5.54 µg/L y una linealidad en el rango de 5.54 µg/L a 100 µg/L, adicionalmente, se evaluó exactitud y precisión, obteniendo resultados favorables para el análisis. El cálculo de la incertidumbre expandida para este método fue 0.610 µg/L.application/pdfspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/rcolquim/article/view/45909Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de QuímicaRevista Colombiana de QuímicaRevista Colombiana de Química; Vol. 42, núm. 3 (2013): Versión preliminar; 326-343 2357-3791 0120-2804Ramírez, Carolina and Olarte, Ermel and Tellez, Eliana and Palma, Marien (2013) Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+. Revista Colombiana de Química; Vol. 42, núm. 3 (2013): Versión preliminar; 326-343 2357-3791 0120-2804 .Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTmercuriovalidación de métodosespectrofotometría de absorción atómicamercuryanalytical methods validationatomic absorption spectrophotometryORIGINAL45909-222616-1-SM.pdfapplication/pdf1022300https://repositorio.unal.edu.co/bitstream/unal/50024/1/45909-222616-1-SM.pdf50c13d8fba975a071d533817bebe9bbcMD51THUMBNAIL45909-222616-1-SM.pdf.jpg45909-222616-1-SM.pdf.jpgGenerated Thumbnailimage/jpeg6226https://repositorio.unal.edu.co/bitstream/unal/50024/2/45909-222616-1-SM.pdf.jpgd9e5d7e21eca4f07ec7221bf393f3d23MD52unal/50024oai:repositorio.unal.edu.co:unal/500242022-12-18 23:06:29.662Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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