Analytical method validation for determination of total mercury in human blood by zeeman mercury spectrometer ra-915+ with the pyrolysis module pyro-915+

This article describes the methodology of the validation about the identification of total mercury in blood samples by Zeeman atomic absorption spectrophotometry RA-915+ with the module of Pyrolysis PYRO-915+, suitable for the direct determination of total mercury concentrations in various matrices, that unlike other atomization techniques (graphite furnace, cold vapor or hydride generator) does not require a sample pre-treatment. With the application of this validation, is provided the reliability in the results. Most of the validation parameters were subjected to analysis of variance ANOVA with a confidence level of 95%. It was obtained a detection limit of 2.16 µg/L, a quantitation limit of 5.54µg/L and linearity in the range of 5.54 µg/L to , in addition, the precision and accuracy was evaluated, obtaining favorable results in the analysis. The calculation of the expanded uncertainty for this method was 0.610 µg/L.

• Autores: Ramírez, Carolina; Olarte, Ermel; Tellez, Eliana; Palma, Marien
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Universidad Nacional de Colombia
• Repositorio: Universidad Nacional de Colombia
• Idioma: spa
• OAI Identifier: oai:repositorio.unal.edu.co:unal/50024
• Acceso en línea: https://repositorio.unal.edu.co/handle/unal/50024; http://bdigital.unal.edu.co/43552/
• Palabra clave: mercurio; validación de métodos; espectrofotometría de absorción atómica; mercury; analytical methods validation; atomic absorption spectrophotometry
• Rights: openAccess
• License: Atribución-NoComercial 4.0 Internacional