Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.

ilustraciones, diagramas

Autores:: Rodriguez Abril, Edna Yadira

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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repository_id_str
dc.title.spa.fl_str_mv	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
dc.title.translated.eng.fl_str_mv	Evaluation of the in vitro behavior of soybean genotypes during genetic transformation mediated by Agrobacterium tumefaciens.
title	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
spellingShingle	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens. 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Soybean Soya In vitro culture Ciltivo in vitro Regeneración in vitro Genotipos Regeneración Transformación Glifosato Genotype Regeneration Transformation Glyphosate
title_short	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
title_full	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
title_fullStr	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
title_full_unstemmed	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
title_sort	Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
dc.creator.fl_str_mv	Rodriguez Abril, Edna Yadira
dc.contributor.advisor.none.fl_str_mv	Soto Sedano, Johana Carolina Chaparro Giraldo, Alejandro
dc.contributor.author.none.fl_str_mv	Rodriguez Abril, Edna Yadira
dc.contributor.researchgroup.spa.fl_str_mv	Ingeniería Genética de Plantas
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
topic	630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Soybean Soya In vitro culture Ciltivo in vitro Regeneración in vitro Genotipos Regeneración Transformación Glifosato Genotype Regeneration Transformation Glyphosate
dc.subject.lemb.none.fl_str_mv	Soybean Soya In vitro culture Ciltivo in vitro Regeneración in vitro
dc.subject.proposal.spa.fl_str_mv	Genotipos Regeneración Transformación Glifosato
dc.subject.proposal.eng.fl_str_mv	Genotype Regeneration Transformation Glyphosate
description	ilustraciones, diagramas
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-10-16T15:24:25Z
dc.date.available.none.fl_str_mv	2021-10-16T15:24:25Z
dc.date.issued.none.fl_str_mv	2021-11-09
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
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dc.type.content.spa.fl_str_mv	Text
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status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/80567
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
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url	https://repositorio.unal.edu.co/handle/unal/80567 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.references.spa.fl_str_mv	Anami, S., Njuguna, E., Coussens, G., Aesaert, S., & Van Lijsebettens, M. (2013). Higher plant transformation: Principles and molecular tools. International Journal of Developmental Biology, 57(6–8), 483–494. https://doi.org/10.1387/ijdb.130232mv Barfoot, P., & Brookes, G. (2014). Key global environmental impacts of genetically modified (GM) crop use 1996-2012. GM Crops & Food, 5(2), 149–160. https://doi.org/10.4161/gmcr.28449 Barry, G., Kishore, G., Padgette, S., & Talling, W. (1997). Patente no US 6248876 B1. USA. Caicedo Guerrero, S., Tibocha Ardila, Y., Campuzano Duque, L. F., Flórez Gómez, D. L., & Arguelles Cardenas, J. (2020). Productive performance of seven soybeans genotypes in acid soils of the Colombian Orinoquía. Agronomy Mesoamerican, 31(1), 59–68. https://doi.org/10.15517/AM.V31I1.34440 Christianson, M. L., Warnick, D. A., & Carlson, P. S. (1983). A Morphogenetically Competent Soybean Suspension Culture Abstract. SCIENCE, 222(7), 632–634. Clemente, T. E., Lavallee, B. J., Howe, A. R., Conner-ward, D., Rozman, R. J., Hunter, P. E., … Hinchee, M. A. (2000). Progeny Analysis of Glyphosate Selected Transgenic Soybeans Derived from Agrobacterium-Mediated Transformation. CROP SCIENCE, 40, 797–803 Donaldson, P. A., & Simmonds, D. H. (2000). Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean. Plant Cell Reports, 19(5), 478–484. https://doi.org/10.1007/s002990050759 Droste, A., Pasquali, G., & Bodanese-Zanettini, M. H. (2002). Transgenic fertile plants of soybean [Glycine max (L.) Merrill] obtained from bombarded embryogenic tissue. Euphytica, 127(3), 367–376. https://doi.org/10.1023/A:1020370913140 Galindo, L. (2019). Establecimiento de una curva de selección in vitro con glifosato en un sistema de regeneración de tres variedades colombianas de soya (Glycine Max (L).Merrill). Universidad INCCA de Colombia. Gelvin, S. B. (2003). Agrobacterium-Mediated Plant Transformation: the Biology behind the " Gene-Jockeying " Tool. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 67(1), 16–37. https://doi.org/10.1128/MMBR.67.1.16–37.2003 Gelvin, S. B. (2012). Traversing the cell : Agrobacterium T-DNA ’ s journey to the host genome, 3(March), 1–11. https://doi.org/10.3389/fpls.2012.00052 Heringer, A. S., Santa-Catarina, C., & Silveira, V. (2018). Insights from Proteomic Studies into Plant Somatic Embryogenesis. Proteomics, 18(5–6), 1–25. https://doi.org/10.1002/pmic.201700265 Hinchee, M. A. W., Connor-Ward, D. V., Newell, C. A., McDonnell, R. E., Sato, S. J., Gasser, C. S., … Horsch, R. B. (1988). Production of transgenic soybean plants using agrobacterium-mediated DNA transfer. Bio/Technology, 6(8), 915–922. https://doi.org/10.1038/nbt0888-915 ISAAA. (2019). ISAAA Brief 55-2019: Executive Summary Biotech Crops Drive Socio-Economic Development and Sustainable Environment in the New Frontier. 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H., Li, W., Zhang, C., Yang, L., Chang, R. Z., Gaut, B. S., & Qiu, L. J. (2010). Genetic diversity in domesticated soybean (Glycine max) and its wild progenitor (Glycine soja) for simple sequence repeat and single-nucleotide polymorphism loci. New Phytologist, 188(1), 242–253. https://doi.org/10.1111/j.1469-8137.2010.03344.x Ma, X. H., & Wu, T. L. (2008). Rapid and efficient regeneration in soybean [Glycine max (L.) Merrill] from whole cotyledonary node explants. Acta Physiologiae Plantarum, 30(2), 209–216. https://doi.org/10.1007/s11738-007-0109-3 Miki, B., & McHugh, S. (2004). Selectable marker genes in transgenic plants: Applications, alternatives and biosafety. Journal of Biotechnology, 107(3), 193–232. https://doi.org/10.1016/j.jbiotec.2003.10.011 Ministerio de Agricultura y Desarrollo Rural. (2019). Soya. Direccion de cadenas agricolas y forestales. Nutrition & Food Science. https://doi.org/10.1108/nfs.2012.01742eaa.013 Olhoft, P. M., & Somers, D. A. (2001). L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells. Plant Cell Reports, 20(8), 706–711. https://doi.org/10.1007/s002990100379 Paes de Melo, B., Lourenço-Tessutti, I. T., Morgante, C. V., Santos, N. C., Pinheiro, L. B., de Jesus Lins, C. B., … Grossi-de-Sa, M. F. (2020). Soybean Embryonic Axis Transformation: Combining Biolistic and Agrobacterium-Mediated Protocols to Overcome Typical Complications of In Vitro Plant Regeneration. Frontiers in Plant Science, 11(August), 1–14. https://doi.org/10.3389/fpls.2020.01228 Pareddy, D., Chennareddy, S., Anthony, G., Sardesai, N., Mall, T., Minnicks, T., … Sarria, R. (2020). Improved soybean transformation for efficient and high throughput transgenic production. Transgenic Research, 29(3), 267–281. https://doi.org/10.1007/s11248-020-00198-8 Parrott, W. A., Hoffman, L. M., Hildebrand, D. F., Williams, E. G., & Collins, G. B. (1989). Recovery of primary transformants of soybean. Plant Cell Reports, 7, 615–617. Paz, M. M., Martinez, J. C., Kalvig, A. B., Fonger, T. M., & Wang, K. (2006). Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation. Plant Cell Reports, 25(3), 206–213. Paz, M. M., Shou, H., Guo, Z., Zhang, Z., Banerjee, A. K., & Wang, K. (2004). Assessment of conditions affecting. Transformation, 167–179. Qiu, L. J., Xing, L. L., Guo, Y., Wang, J., Jackson, S. A., & Chang, R. Z. (2013). A platform for soybean molecular breeding: The utilization of core collections for food security. Plant Molecular Biology, 83(1–2), 41–50. https://doi.org/10.1007/s11103-013-0076-6 Raza, G., Singh, M. B., & Bhalla, P. L. (2017). In Vitro Plant Regeneration from Commercial Cultivars of Soybean. BioMed Research International, 2017. https://doi.org/10.1155/2017/7379693 Raza, G., Singh, M. B., & Bhalla, P. L. (2020). Somatic embryogenesis and plant regeneration from commercial soybean cultivars. Plants, 9(1). https://doi.org/10.3390/plants9010038 Reddy, M. S. S., Dinkins, R. D., & Collins, G. B. (2003). Gene silencing in transgenic soybean plants transformed via particle bombardment. Plant Cell Reports, 21(7), 676–683. https://doi.org/10.1007/s00299-002-0567-4 Reichert, N. A., Young, M. M., & Woods, A. (2003). Adventitious organogenic regeneration from soybean genotypes representing nine maturity groups. Plant Cell, Tissue and Organ Culture, 75, 273–277. Ribichich Karina F. Mariana Chiozza, Selva Ávalos-Britez, Julieta V. Cabello, Augustin L. Arce, Geronimo Watson, Claudia Arias , Margarita Portapila , Federico Trucco , Maria E. Otegui and Raquel L. Chan. Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4. Journal of Experimental Botany, Vol. 71, No. 10 pp. 3142–3156, 2020 doi:10.1093/jxb/eraa064. Ridner, E. (2006). Soja, propiedades nutricionales y su impacto en la salud. Chemistry & …. Rojas, A., Lopez Pazos, S., & Chaparro Giraldo, A. (2018). Screening of colombian soybean genotypes for agrobacterium mediated genetic transformation conferring tolerance to glyphosate. Agronomia Colombiana, 36(1), 24–34. https://doi.org/10.15446/agron.colomb.v36n1.67440 Rose, R. J. (2019). Somatic embryogenesis in the Medicago truncatula model: Cellular and molecular mechanisms. Frontiers in Plant Science, 10(March). https://doi.org/10.3389/fpls.2019.00267 Rüdelsheim, P., Dumont, P., Freyssinet, G., Pertry, I., & Heijde, M. (2018). Off-Patent Transgenic Events: Challenges and Opportunities for New Actors and Markets in Agriculture. Frontiers in bioengineering and biotechnology, 6, 71. https://doi.org/10.3389/fbioe.2018.00071 Salammal, T., Ramesh, V., Jiang, S.-Y., Ganapathi, A., & Ramachandr, S. (2013). In vitro Regeneration and Genetic Transformation of Soybean: Current Status and Future Prospects. A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships. https://doi.org/10.5772/54268 Siehl Daniel L, Yumin Tao , Henrik Albert , Yuxia Dong , Matthew Heckert , Alfredo Madrigal , Brishette Lincoln-Cabatu , Jian Lu , Tamara Fenwick , Ericka Bermudez , Marian Sandoval , Caroline Horn , Jerry M Green , Theresa Hale , Peggy Pagano , Jenna Clark , Ingrid A Udranszky , Nancy Rizzo , Timothy Bourett , Richard J Howard , David H Johnson , Mark Vogt , Goke Akinsola , Linda Castle. Broad 4-hydroxyphenylpyruvate dioxygenase inhibitor herbicide tolerance in soybean with an optimized enzyme and expression cassette. Plant Physiol. 2014 Nov;166(3):1162-76. doi: 10.1104/pp.114.247205 Shou, H., Frame, B. R., Whitham, S. A., & Wang, K. (2004). Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation. 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In vitro regeneration of soybean plants of the Cuban Incasoy-36 variety. Biotecnologia Aplicada, 30(1), 34–38. Steward, F. C., Mapes, M. O., & Mears, K. (1958). GROWTH AND ORGANIZED DEVELOPMENT OF CULTURED CELLS. II. Organization in Cultures Grown from Freely Suspended Cell. American Journal of Botany, 45(10), 705–708. https://doi.org/10.1002/j.1537-2197.1958.tb10599.x Sundar, I. K., & Sakthivel, N. (2008). Advances in selectable marker genes for plant transformation. Journal of Plant Physiology, 165(16), 1698–1716. https://doi.org/10.1016/j.jplph.2008.08.002 Travella, S., Ross, S. M., Harden, J., Everett, C., Snape, J. W., & Harwood, W. A. (2005). A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Reports, 23(12), 780–789. https://doi.org/10.1007/s00299-004-0892-x Valencia R., R., Carmen C, H., Vargas, H., & Arrieta, G. (2006). Variedades mejoradas de soya para zonas productoras actuales y potenciales de Colombia. Corpoica Ciencia y Tecnología Agropecuaria, 4(2–3), 7–15. Valencia R., R., & Ligarreto M., G. (2010). Soybean breeding (Glycine max [L.] Merril) for the Colombian Altillanura: a prospective and conceptual view. Agronomía Colombiana, 28(2), 155–163. Von Arnold, S., Sabala, I., Bozhkov, P., Dyachok, J., & Filonova, L. (2002). Developmental pathways of somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 69(3), 233–249. https://doi.org/10.1023/A:1015673200621 Yang, S., Hu, Y., Cheng, Z., Rice, J. H., Miao, L., Ma, J., … Gai, J. (2019). An efficient Agrobacterium-mediated soybean transformation method using green fluorescent protein as a selectable marker. Plant Signaling and Behavior, 14(7), 1–7. https://doi.org/10.1080/15592324.2019.1612682 Zeng, P., Vadnais, D. A., Zhang, Z., & Polacco, J. C. (2004). Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill]. Plant Cell Reports. https://doi.org/10.1007/s00299-003-0712-8
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Medellín - Ciencias Agrarias - Maestría en Ciencias Agrarias
dc.publisher.department.spa.fl_str_mv	Departamento de Agronómicas
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias Agrarias
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
institution	Universidad Nacional de Colombia
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Soto Sedano, Johana Carolinaa23116a7e4bfbd576593044f3f2c5741Chaparro Giraldo, Alejandro0352f7295e6de906d5a65eadf07006feRodriguez Abril, Edna Yadira775f170dfa00822cbd5b59a4f7e8807aIngeniería Genética de Plantas2021-10-16T15:24:25Z2021-10-16T15:24:25Z2021-11-09https://repositorio.unal.edu.co/handle/unal/80567Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasEn esta investigación se estudiaron cuatro genotipos de soya colombiana (Brasilera 1, Brasilera 2, FNS01 y Soy SK-7), bajo un sistema de transformación genética mediada por Agrobacterium tumefaciens cepa AGL0, induciendo organogénesis directa a partir de nodo cotiledonar y realizando selección con el herbicida glifosato. Los genotipos fueron evaluados durante las fases in vitro de inducción de brote (IB), elongación de brote (EB) y enraizamiento. Además, se evaluó la etapa de endurecimiento y se determinó la eficiencia de transformación. No se encontraron diferencias significativas entre las cuatro variedades durante la fase in vitro ni durante el endurecimiento. Se obtuvieron transformantes de las cuatro variedades y la variedad Brasilera 1 presentó la mayor eficiencia de transformación con 3.7%. (Texto tomado de la fuente)In this research, four Colombian soybean genotypes (Brasilera 1, Brasilera 2, FNS01 y Soy SK-7) were studied under a system of genetic transformation mediated by Agrobacterium tumefaciens, strain AGL0, inducing direct organogenesis from the cotyledonary node and performing selection with the herbicide glyphosate. Genotypes were evaluated during the in vitro sprout induction (IB), sprout elongation (BE) and rooting phases. In addition, the hardening stage was evaluated, and transformation efficiency was determined. No significant differences were found between the four varieties during the in vitro phases or during hardening. Transformants of the four varieties were obtained, and Brasilera 1 presented the highest transformation efficiency at 3.7%.MaestríaMagister en ciencias agrariasGenética y fitomejoramientoxviii, 55 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias Agrarias - Maestría en Ciencias AgrariasDepartamento de AgronómicasFacultad de Ciencias AgrariasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materialesSoybeanSoyaIn vitro cultureCiltivo in vitroRegeneración in vitroGenotiposRegeneraciónTransformaciónGlifosatoGenotypeRegenerationTransformationGlyphosateEvaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.Evaluation of the in vitro behavior of soybean genotypes during genetic transformation mediated by Agrobacterium tumefaciens.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAnami, S., Njuguna, E., Coussens, G., Aesaert, S., & Van Lijsebettens, M. (2013). Higher plant transformation: Principles and molecular tools. International Journal of Developmental Biology, 57(6–8), 483–494. https://doi.org/10.1387/ijdb.130232mvBarfoot, P., & Brookes, G. (2014). Key global environmental impacts of genetically modified (GM) crop use 1996-2012. GM Crops & Food, 5(2), 149–160. https://doi.org/10.4161/gmcr.28449Barry, G., Kishore, G., Padgette, S., & Talling, W. (1997). Patente no US 6248876 B1. USA.Caicedo Guerrero, S., Tibocha Ardila, Y., Campuzano Duque, L. F., Flórez Gómez, D. L., & Arguelles Cardenas, J. (2020). Productive performance of seven soybeans genotypes in acid soils of the Colombian Orinoquía. Agronomy Mesoamerican, 31(1), 59–68. https://doi.org/10.15517/AM.V31I1.34440Christianson, M. L., Warnick, D. A., & Carlson, P. S. (1983). A Morphogenetically Competent Soybean Suspension Culture Abstract. SCIENCE, 222(7), 632–634.Clemente, T. E., Lavallee, B. J., Howe, A. R., Conner-ward, D., Rozman, R. J., Hunter, P. E., … Hinchee, M. A. (2000). Progeny Analysis of Glyphosate Selected Transgenic Soybeans Derived from Agrobacterium-Mediated Transformation. CROP SCIENCE, 40, 797–803Donaldson, P. A., & Simmonds, D. H. (2000). Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean. Plant Cell Reports, 19(5), 478–484. https://doi.org/10.1007/s002990050759Droste, A., Pasquali, G., & Bodanese-Zanettini, M. H. (2002). Transgenic fertile plants of soybean [Glycine max (L.) Merrill] obtained from bombarded embryogenic tissue. Euphytica, 127(3), 367–376. https://doi.org/10.1023/A:1020370913140Galindo, L. (2019). Establecimiento de una curva de selección in vitro con glifosato en un sistema de regeneración de tres variedades colombianas de soya (Glycine Max (L).Merrill). Universidad INCCA de Colombia.Gelvin, S. B. (2003). Agrobacterium-Mediated Plant Transformation: the Biology behind the " Gene-Jockeying " Tool. 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Plant Cell Reports. https://doi.org/10.1007/s00299-003-0712-8FENALCELICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/80567/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINAL1030532866.pdf1030532866.pdfTesis de Maestría en Ciencias Agrariasapplication/pdf849300https://repositorio.unal.edu.co/bitstream/unal/80567/2/1030532866.pdf17fb6a8a2eeb984651206eb4acc7acb3MD52THUMBNAIL1030532866.pdf.jpg1030532866.pdf.jpgGenerated Thumbnailimage/jpeg5207https://repositorio.unal.edu.co/bitstream/unal/80567/3/1030532866.pdf.jpg9c83cbcb859d7335646f6c36260416cdMD53unal/80567oai:repositorio.unal.edu.co:unal/805672024-07-31 23:13:57.063Repositorio Institucional Universidad Nacional de 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Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.

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