Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas
ilustraciones, gráficas
- Autores:
-
Murillo Murillo, Marisol
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/83973
- Palabra clave:
- 570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales
Privación Materna
Receptores de Cannabinoides/fisiología
Receptores Opioides/fisiología
Maternal Deprivation
Receptors, Cannabinoid/physiology
Receptors, Opioid/physiology
Separación materna durante la lactancia
Receptor opioide Mu
Receptor CB1
Sucralosa
Estrés
Epigenética
Obesidad
Early life stress
CB1 Receptor
Mu opioid receptor
Sucralose
Obesity
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas |
| dc.title.translated.eng.fl_str_mv |
Effect of maternal separation during breastfeeding and sucralose consumption on endocannabinoid receptors and endogenous opioid receptors expression´s in rat brains |
| title |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas |
| spellingShingle |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas 570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales Privación Materna Receptores de Cannabinoides/fisiología Receptores Opioides/fisiología Maternal Deprivation Receptors, Cannabinoid/physiology Receptors, Opioid/physiology Separación materna durante la lactancia Receptor opioide Mu Receptor CB1 Sucralosa Estrés Epigenética Obesidad Early life stress CB1 Receptor Mu opioid receptor Sucralose Obesity |
| title_short |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas |
| title_full |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas |
| title_fullStr |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas |
| title_full_unstemmed |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas |
| title_sort |
Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas |
| dc.creator.fl_str_mv |
Murillo Murillo, Marisol |
| dc.contributor.advisor.spa.fl_str_mv |
Dueñas Gómez, Zulma Janeth |
| dc.contributor.author.spa.fl_str_mv |
Murillo Murillo, Marisol |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales |
| topic |
570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales Privación Materna Receptores de Cannabinoides/fisiología Receptores Opioides/fisiología Maternal Deprivation Receptors, Cannabinoid/physiology Receptors, Opioid/physiology Separación materna durante la lactancia Receptor opioide Mu Receptor CB1 Sucralosa Estrés Epigenética Obesidad Early life stress CB1 Receptor Mu opioid receptor Sucralose Obesity |
| dc.subject.decs.spa.fl_str_mv |
Privación Materna Receptores de Cannabinoides/fisiología Receptores Opioides/fisiología |
| dc.subject.decs.eng.fl_str_mv |
Maternal Deprivation Receptors, Cannabinoid/physiology Receptors, Opioid/physiology |
| dc.subject.proposal.spa.fl_str_mv |
Separación materna durante la lactancia Receptor opioide Mu Receptor CB1 Sucralosa Estrés Epigenética Obesidad |
| dc.subject.proposal.eng.fl_str_mv |
Early life stress CB1 Receptor Mu opioid receptor Sucralose Obesity |
| description |
ilustraciones, gráficas |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-06-05T20:18:24Z |
| dc.date.available.none.fl_str_mv |
2023-06-05T20:18:24Z |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/83973 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/83973 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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Bireme |
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Universidad Nacional de Colombia |
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Bogotá - Medicina - Maestría en Fisiología |
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Facultad de Medicina |
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Bogotá, Colombia |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dueñas Gómez, Zulma Janethddf6ccc29fb0eb2a962c31ef7d7bb79d600Murillo Murillo, Marisol496ccd82712a78dfb590d007011f50e62023-06-05T20:18:24Z2023-06-05T20:18:24Z2023https://repositorio.unal.edu.co/handle/unal/83973Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficasa separación materna durante la lactancia (SMDL) en roedores (modelo de estrés crónico) altera procesos del desarrollo a nivel neuroendocrino y fisiológico, evidentes en la adolescencia y adultez asociados con la depresión, el abuso de sustancias, dificultades en procesos aprendizaje, ansiedad y trastornos de conductas alimentarias. En este sentido sistemas neuromoduladores endocannabinoide y opioides que actúan sobre la actividad del eje Hipotálamo Pituitaria Adrenal e inciden en áreas reguladoras de la conducta alimentaria y sistema de recompensa por acción de receptores cannabinoides CB1 y opioides tipo Mu podrían presentar alteraciones. Por lo anterior, el objetivo de este trabajo investigativo fue determinar el efecto de la SMDL y el consumo de sucralosa sobre la expresión de receptores de CB1 y Mu en corteza prefrontal medial, hipocampo, estriado, área tegmental ventral y la amígdala de ratas wistar adolescentes de ambos sexos. Metodología: Se utilizaron 35 ratas Wistar machos y hembras divididas en grupos con y sin SMDL (n=18; n:17) durante los días posnatal (DPN) 1 a las 21 3 horas en la mañana y 3 en la tarde. El DPN 22 se destetaron, separaron por sexo y se brindó alimentación y agua ad-libitum; el DPN 27- 52 se ofreció consumo de edulcorante (sucralosa) y agua; el DPN 53 se sacrificaron con perfusión transcardiaca, remoción de encéfalo y posteriormente se realizó el corte e inmunohistoquímica en áreas seleccionadas, toma de microfotografía, medición de densitometría y análisis estadístico (test Kruskall Wallis; software R, P= 0.05). Resultados: existen cambios diferenciales en la expresión de receptores cannabinoides CB1 y opioides tipo Mu en áreas analizadas en condiciones estándar de crianza y alimentación, así mismo dicha expresión tiende a alterarse de manera diferencial dependiente del sexo posterior a la SMDL y el consumo de sucralosa de manera independiente y conjunta, tendientes a aumentar su expresión en machos y disminuirla en hembras. Conclusión: La SMDL genera alteración en receptores CB1 y Mu en areas neuromoduladores del eje HPA y sistema de recompensa; así mismo, el posterior consumo de sucralosa exacerba el efecto de la SMDL. (Texto tomado de la fuente).Maternal separation during breastfeeding (MSDB) in rodents (chronic stress model) alters developmental processes at neuroendocrine and physiological levels, evident in adolescence and adulthood associated with depression, substance abuse, learning difficulties, anxiety and eating disorders. In this sense, endocannabinoid and opioid neuromodulatory systems that act on the activity of the hypothalamic-pituitary-adrenal axis and affect regulatory areas of eating behavior and reward system through the action of CB1 cannabinoid receptors and Mu-type opioids could present alterations. Therefore, the aim of this research was to determine the effect of MSDB and sucralose consumption on the expression of CB1 and Mu receptors in the medial prefrontal cortex, hippocampus, striatum, ventral tegmental area and amygdala of adolescent wistar rats of both sexes. Methodology: 35 male and female Wistar rats divided into groups with and without MSDB (n=18; n:17) were used during postnatal days (PND) 1 at 21 3 hours in the morning and 3 in the afternoon. On PND 22 they were weaned, separated by sex and fed and water was provided ad-libitum; on PND 27- 52 they were offered sweetener consumption (sucralose) and water; on PND 53 they were sacrificed with transcardiac perfusion, removal of the encephalon and then cut and immunohistochemistry was performed in selected areas, microphotography, densitometry measurement and statistical analysis (Kruskall Wallis test; R software, P= 0.05). Results: there are differential changes in the expression of CB1 cannabinoid and Mu-type opioid receptors in areas analyzed under standard rearing and feeding conditions. Likewise, such expression tends to alter in a sex-dependent differential manner after MSDB and sucralose consumption independently and jointly, tending to increase its expression in males and decrease it in females. Conclusion: MSDB generates alterations in CB1 and Mu receptors in neuromodulatory areas of the HPA axis and reward system and the subsequent sucralose consumption exacerbates the effect of MSDB.MaestríaMagíster en FisiologíaNeurofisiología comportamentalxi, 84 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en FisiologíaFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animalesPrivación MaternaReceptores de Cannabinoides/fisiologíaReceptores Opioides/fisiologíaMaternal DeprivationReceptors, Cannabinoid/physiologyReceptors, Opioid/physiologySeparación materna durante la lactanciaReceptor opioide MuReceptor CB1SucralosaEstrésEpigenéticaObesidadEarly life stressCB1 ReceptorMu opioid receptorSucraloseObesityEfecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratasEffect of maternal separation during breastfeeding and sucralose consumption on endocannabinoid receptors and endogenous opioid receptors expression´s in rat brainsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBiremeAhmed, E. 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Acta Pharmacologica Sinica, 40(3), Art. 3. https://doi.org/10.1038/s41401-018-0051-5EstudiantesInvestigadoresMaestrosMedios de comunicaciónPadres y familiasPersonal de apoyo escolarPúblico generalResponsables políticosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83973/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53ORIGINAL1106778186.2023.pdf1106778186.2023.pdfTesis de Maestría en Fisiologíaapplication/pdf4418167https://repositorio.unal.edu.co/bitstream/unal/83973/4/1106778186.2023.pdf0b26b40ab724228bf319068f9dfba1f6MD54THUMBNAIL1106778186.2023.pdf.jpg1106778186.2023.pdf.jpgGenerated Thumbnailimage/jpeg5044https://repositorio.unal.edu.co/bitstream/unal/83973/5/1106778186.2023.pdf.jpgd8a2c1ff0dcf64fac980f9223ab00423MD55unal/83973oai:repositorio.unal.edu.co:unal/839732023-08-08 23:04:00.461Repositorio Institucional Universidad Nacional de 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