Next generation sequence analysis of the forage peanut (Arachis pintoi) virome

Forage peanut (Arachis pintoi) is one of the forage crops best adapted to tropical agroecosystems where it is used as ground cover in urban areas and slopes, in the preservation of soils cultivated with coffee, African oil palm and citrus and as animal feed in combination with gramineous plants. A....

Full description

Autores:
Gutiérrez Sánchez, Pablo Andrés
Jaramillo Mesa, Helena
Marín Montoya, Mauricio
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/58382
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/58382
http://bdigital.unal.edu.co/55164/
Palabra clave:
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
Alphaflexiviridae
Polerovirus
Potyvirus
Viral genomes
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_2d38501b7c03020ef3c35a087cb102e4
oai_identifier_str oai:repositorio.unal.edu.co:unal/58382
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gutiérrez Sánchez, Pablo Andrés8324cb8e-9fd5-44ea-a44f-8e8184a2ffd8300Jaramillo Mesa, Helena7c8f3a27-994b-44d6-a3af-a388f7ca3ef8300Marín Montoya, Mauricio936d15fc-c0c5-45c6-b61c-b1c548ee7e803002019-07-02T14:05:55Z2019-07-02T14:05:55Z2016-07-01ISSN: 2248-7026https://repositorio.unal.edu.co/handle/unal/58382http://bdigital.unal.edu.co/55164/Forage peanut (Arachis pintoi) is one of the forage crops best adapted to tropical agroecosystems where it is used as ground cover in urban areas and slopes, in the preservation of soils cultivated with coffee, African oil palm and citrus and as animal feed in combination with gramineous plants. A. pintoi is considered to be highly resistant to plagues and diseases; however, in recent years there has been a marked increase of plants showing symptoms typical of viral infection. In this work, Next Generation Sequencing (NGS) was used to confirm the presence of virus in symptomatic A. pintoi plants collected in urban areas in Medellín (Colombia). Transcriptome analysis revealed the presence of 3,291,089 reads related to viruses in the families Potyviridae, Luteoviridae and Alphaflexiviridae and resulted in the complete genome assembly of Peanut mottle virus (9707 nt), Turnip yellows virus (5578 nt) and two variants of a virus with phylogenetic affinity to the genus Allexivirus. These two variants lack ORF6 present in Allexivirus and probably belong to an uncharacterized genus within the family Alphaflexiviridae. The presence of at least three viruses infecting A. pintoi in Colombia highlights the importance of starting a germplasm clean-up program of the plant material used as seed in this crop.application/pdfspaUniversidad Nacional de Colombiahttp://www.revistas.unal.edu.co/index.php/refame/article/view/59133Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía MedellínRevista Facultad Nacional de Agronomía MedellínGutiérrez Sánchez, Pablo Andrés and Jaramillo Mesa, Helena and Marín Montoya, Mauricio (2016) Next generation sequence analysis of the forage peanut (Arachis pintoi) virome. Revista Facultad Nacional de Agronomía, 69 (2). pp. 7881-7891. ISSN 2248-702657 Ciencias de la vida; Biología / Life sciences; biology58 Plantas / PlantsAlphaflexiviridaePolerovirusPotyvirusViral genomesNext generation sequence analysis of the forage peanut (Arachis pintoi) viromeArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL59133-300467-2-PB.pdfapplication/pdf945299https://repositorio.unal.edu.co/bitstream/unal/58382/1/59133-300467-2-PB.pdf87c272ef9d4a617b68caa5e8cf16cc61MD51THUMBNAIL59133-300467-2-PB.pdf.jpg59133-300467-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg7893https://repositorio.unal.edu.co/bitstream/unal/58382/2/59133-300467-2-PB.pdf.jpgc0298ead9588108d1aed7193693312e1MD52unal/58382oai:repositorio.unal.edu.co:unal/583822024-04-01 23:08:11.683Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
title Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
spellingShingle Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
Alphaflexiviridae
Polerovirus
Potyvirus
Viral genomes
title_short Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
title_full Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
title_fullStr Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
title_full_unstemmed Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
title_sort Next generation sequence analysis of the forage peanut (Arachis pintoi) virome
dc.creator.fl_str_mv Gutiérrez Sánchez, Pablo Andrés
Jaramillo Mesa, Helena
Marín Montoya, Mauricio
dc.contributor.author.spa.fl_str_mv Gutiérrez Sánchez, Pablo Andrés
Jaramillo Mesa, Helena
Marín Montoya, Mauricio
dc.subject.ddc.spa.fl_str_mv 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
topic 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
Alphaflexiviridae
Polerovirus
Potyvirus
Viral genomes
dc.subject.proposal.spa.fl_str_mv Alphaflexiviridae
Polerovirus
Potyvirus
Viral genomes
description Forage peanut (Arachis pintoi) is one of the forage crops best adapted to tropical agroecosystems where it is used as ground cover in urban areas and slopes, in the preservation of soils cultivated with coffee, African oil palm and citrus and as animal feed in combination with gramineous plants. A. pintoi is considered to be highly resistant to plagues and diseases; however, in recent years there has been a marked increase of plants showing symptoms typical of viral infection. In this work, Next Generation Sequencing (NGS) was used to confirm the presence of virus in symptomatic A. pintoi plants collected in urban areas in Medellín (Colombia). Transcriptome analysis revealed the presence of 3,291,089 reads related to viruses in the families Potyviridae, Luteoviridae and Alphaflexiviridae and resulted in the complete genome assembly of Peanut mottle virus (9707 nt), Turnip yellows virus (5578 nt) and two variants of a virus with phylogenetic affinity to the genus Allexivirus. These two variants lack ORF6 present in Allexivirus and probably belong to an uncharacterized genus within the family Alphaflexiviridae. The presence of at least three viruses infecting A. pintoi in Colombia highlights the importance of starting a germplasm clean-up program of the plant material used as seed in this crop.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-07-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T14:05:55Z
dc.date.available.spa.fl_str_mv 2019-07-02T14:05:55Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.issn.spa.fl_str_mv ISSN: 2248-7026
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/58382
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/55164/
identifier_str_mv ISSN: 2248-7026
url https://repositorio.unal.edu.co/handle/unal/58382
http://bdigital.unal.edu.co/55164/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://www.revistas.unal.edu.co/index.php/refame/article/view/59133
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía Medellín
Revista Facultad Nacional de Agronomía Medellín
dc.relation.references.spa.fl_str_mv Gutiérrez Sánchez, Pablo Andrés and Jaramillo Mesa, Helena and Marín Montoya, Mauricio (2016) Next generation sequence analysis of the forage peanut (Arachis pintoi) virome. Revista Facultad Nacional de Agronomía, 69 (2). pp. 7881-7891. ISSN 2248-7026
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/58382/1/59133-300467-2-PB.pdf
https://repositorio.unal.edu.co/bitstream/unal/58382/2/59133-300467-2-PB.pdf.jpg
bitstream.checksum.fl_str_mv 87c272ef9d4a617b68caa5e8cf16cc61
c0298ead9588108d1aed7193693312e1
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
_version_ 1806886642172035072

Publicaciones similares