Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus

Sixty two virus-free accessions of Solanum phureja from the Colombiancentral collection of this tuber (Bogotá-Soacha) weretested for their susceptibility to infection with Potato yellowvein virus (PYVV). Under greenhouse conditions, the plantswere inoculated with PYVV using viruliferous Trialeurodes...

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Autores:
Guzmàn, Mònica
Rodriguez, Patricia
Tipo de recurso:
Article of journal
Fecha de publicación:
2010
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/31499
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/31499
http://bdigital.unal.edu.co/21578/
http://bdigital.unal.edu.co/21578/2/
Palabra clave:
Crinivirus
PYVV
Potato virus
Trialeurodes vaporariorum.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Guzmàn, Mònicadc613356-68c4-43d7-a519-ebc1431d2bea300Rodriguez, Patricia2db93a46-8d25-4d33-b472-cd452e80e0423002019-06-26T14:34:50Z2019-06-26T14:34:50Z2010https://repositorio.unal.edu.co/handle/unal/31499http://bdigital.unal.edu.co/21578/http://bdigital.unal.edu.co/21578/2/Sixty two virus-free accessions of Solanum phureja from the Colombiancentral collection of this tuber (Bogotá-Soacha) weretested for their susceptibility to infection with Potato yellowvein virus (PYVV). Under greenhouse conditions, the plantswere inoculated with PYVV using viruliferous Trialeurodesvaporariorum (Westwood) whiteflies, and monitored for threemonths (the tuber formation period of S. phureja) for symptomsof infection and virus capsid protein (CP) production, by meansof a reverse transcription-polymerase chain reaction (RT-PCR)assay. Symptoms of PYVV infection, consisting in the chloroticflecking of leaflets, followed by complete vein yellowing, werenoted in 38 accessions whose RNA extracts generated correctsizedCP amplicons in RT-PCR assays. Thirteen of the remainingaccessions were found to be latently infected with the virusthrough positive RT-PCR assays. Twelve accessions were freeof virus infection as judged by the absence of symptoms andnegative RT-PCR assay results over two cycles of tuber settingand germination. These accessions may possess field resistanceto PYVV. This work is the first one to detect and define the levelsof susceptibility of Colombian S. phureja accessions to PYVVinfection, and to identify the potential existence of resistanceto this deleterious Andean potato virusapplication/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/18065Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965Guzmàn, Mònica and Rodriguez, Patricia (2010) Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus. Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965 .Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virusArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTCrinivirusPYVVPotato virusTrialeurodes vaporariorum.ORIGINAL18065-58306-1-PB.pdfapplication/pdf868872https://repositorio.unal.edu.co/bitstream/unal/31499/1/18065-58306-1-PB.pdf6ef36dc8fb3459fb002ad05e486fd143MD51THUMBNAIL18065-58306-1-PB.pdf.jpg18065-58306-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8144https://repositorio.unal.edu.co/bitstream/unal/31499/2/18065-58306-1-PB.pdf.jpg812ba79eb2557d7c43e5dbb268256ce5MD52unal/31499oai:repositorio.unal.edu.co:unal/314992022-12-02 23:04:14.931Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
title Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
spellingShingle Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
Crinivirus
PYVV
Potato virus
Trialeurodes vaporariorum.
title_short Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
title_full Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
title_fullStr Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
title_full_unstemmed Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
title_sort Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
dc.creator.fl_str_mv Guzmàn, Mònica
Rodriguez, Patricia
dc.contributor.author.spa.fl_str_mv Guzmàn, Mònica
Rodriguez, Patricia
dc.subject.proposal.spa.fl_str_mv Crinivirus
PYVV
Potato virus
Trialeurodes vaporariorum.
topic Crinivirus
PYVV
Potato virus
Trialeurodes vaporariorum.
description Sixty two virus-free accessions of Solanum phureja from the Colombiancentral collection of this tuber (Bogotá-Soacha) weretested for their susceptibility to infection with Potato yellowvein virus (PYVV). Under greenhouse conditions, the plantswere inoculated with PYVV using viruliferous Trialeurodesvaporariorum (Westwood) whiteflies, and monitored for threemonths (the tuber formation period of S. phureja) for symptomsof infection and virus capsid protein (CP) production, by meansof a reverse transcription-polymerase chain reaction (RT-PCR)assay. Symptoms of PYVV infection, consisting in the chloroticflecking of leaflets, followed by complete vein yellowing, werenoted in 38 accessions whose RNA extracts generated correctsizedCP amplicons in RT-PCR assays. Thirteen of the remainingaccessions were found to be latently infected with the virusthrough positive RT-PCR assays. Twelve accessions were freeof virus infection as judged by the absence of symptoms andnegative RT-PCR assay results over two cycles of tuber settingand germination. These accessions may possess field resistanceto PYVV. This work is the first one to detect and define the levelsof susceptibility of Colombian S. phureja accessions to PYVVinfection, and to identify the potential existence of resistanceto this deleterious Andean potato virus
publishDate 2010
dc.date.issued.spa.fl_str_mv 2010
dc.date.accessioned.spa.fl_str_mv 2019-06-26T14:34:50Z
dc.date.available.spa.fl_str_mv 2019-06-26T14:34:50Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/21578/
http://bdigital.unal.edu.co/21578/2/
url https://repositorio.unal.edu.co/handle/unal/31499
http://bdigital.unal.edu.co/21578/
http://bdigital.unal.edu.co/21578/2/
dc.language.iso.spa.fl_str_mv spa
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dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/agrocol/article/view/18065
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.ispartofseries.none.fl_str_mv Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965
dc.relation.references.spa.fl_str_mv Guzmàn, Mònica and Rodriguez, Patricia (2010) Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus. Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial
institution Universidad Nacional de Colombia
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