Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus
Sixty two virus-free accessions of Solanum phureja from the Colombiancentral collection of this tuber (Bogotá-Soacha) weretested for their susceptibility to infection with Potato yellowvein virus (PYVV). Under greenhouse conditions, the plantswere inoculated with PYVV using viruliferous Trialeurodes...
- Autores:
-
Guzmàn, Mònica
Rodriguez, Patricia
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2010
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/31499
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/31499
http://bdigital.unal.edu.co/21578/
http://bdigital.unal.edu.co/21578/2/
- Palabra clave:
- Crinivirus
PYVV
Potato virus
Trialeurodes vaporariorum.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Guzmàn, Mònicadc613356-68c4-43d7-a519-ebc1431d2bea300Rodriguez, Patricia2db93a46-8d25-4d33-b472-cd452e80e0423002019-06-26T14:34:50Z2019-06-26T14:34:50Z2010https://repositorio.unal.edu.co/handle/unal/31499http://bdigital.unal.edu.co/21578/http://bdigital.unal.edu.co/21578/2/Sixty two virus-free accessions of Solanum phureja from the Colombiancentral collection of this tuber (Bogotá-Soacha) weretested for their susceptibility to infection with Potato yellowvein virus (PYVV). Under greenhouse conditions, the plantswere inoculated with PYVV using viruliferous Trialeurodesvaporariorum (Westwood) whiteflies, and monitored for threemonths (the tuber formation period of S. phureja) for symptomsof infection and virus capsid protein (CP) production, by meansof a reverse transcription-polymerase chain reaction (RT-PCR)assay. Symptoms of PYVV infection, consisting in the chloroticflecking of leaflets, followed by complete vein yellowing, werenoted in 38 accessions whose RNA extracts generated correctsizedCP amplicons in RT-PCR assays. Thirteen of the remainingaccessions were found to be latently infected with the virusthrough positive RT-PCR assays. Twelve accessions were freeof virus infection as judged by the absence of symptoms andnegative RT-PCR assay results over two cycles of tuber settingand germination. These accessions may possess field resistanceto PYVV. This work is the first one to detect and define the levelsof susceptibility of Colombian S. phureja accessions to PYVVinfection, and to identify the potential existence of resistanceto this deleterious Andean potato virusapplication/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/18065Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965Guzmàn, Mònica and Rodriguez, Patricia (2010) Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus. Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965 .Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virusArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTCrinivirusPYVVPotato virusTrialeurodes vaporariorum.ORIGINAL18065-58306-1-PB.pdfapplication/pdf868872https://repositorio.unal.edu.co/bitstream/unal/31499/1/18065-58306-1-PB.pdf6ef36dc8fb3459fb002ad05e486fd143MD51THUMBNAIL18065-58306-1-PB.pdf.jpg18065-58306-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8144https://repositorio.unal.edu.co/bitstream/unal/31499/2/18065-58306-1-PB.pdf.jpg812ba79eb2557d7c43e5dbb268256ce5MD52unal/31499oai:repositorio.unal.edu.co:unal/314992022-12-02 23:04:14.931Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus |
title |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus |
spellingShingle |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus Crinivirus PYVV Potato virus Trialeurodes vaporariorum. |
title_short |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus |
title_full |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus |
title_fullStr |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus |
title_full_unstemmed |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus |
title_sort |
Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus |
dc.creator.fl_str_mv |
Guzmàn, Mònica Rodriguez, Patricia |
dc.contributor.author.spa.fl_str_mv |
Guzmàn, Mònica Rodriguez, Patricia |
dc.subject.proposal.spa.fl_str_mv |
Crinivirus PYVV Potato virus Trialeurodes vaporariorum. |
topic |
Crinivirus PYVV Potato virus Trialeurodes vaporariorum. |
description |
Sixty two virus-free accessions of Solanum phureja from the Colombiancentral collection of this tuber (Bogotá-Soacha) weretested for their susceptibility to infection with Potato yellowvein virus (PYVV). Under greenhouse conditions, the plantswere inoculated with PYVV using viruliferous Trialeurodesvaporariorum (Westwood) whiteflies, and monitored for threemonths (the tuber formation period of S. phureja) for symptomsof infection and virus capsid protein (CP) production, by meansof a reverse transcription-polymerase chain reaction (RT-PCR)assay. Symptoms of PYVV infection, consisting in the chloroticflecking of leaflets, followed by complete vein yellowing, werenoted in 38 accessions whose RNA extracts generated correctsizedCP amplicons in RT-PCR assays. Thirteen of the remainingaccessions were found to be latently infected with the virusthrough positive RT-PCR assays. Twelve accessions were freeof virus infection as judged by the absence of symptoms andnegative RT-PCR assay results over two cycles of tuber settingand germination. These accessions may possess field resistanceto PYVV. This work is the first one to detect and define the levelsof susceptibility of Colombian S. phureja accessions to PYVVinfection, and to identify the potential existence of resistanceto this deleterious Andean potato virus |
publishDate |
2010 |
dc.date.issued.spa.fl_str_mv |
2010 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-26T14:34:50Z |
dc.date.available.spa.fl_str_mv |
2019-06-26T14:34:50Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/31499 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/21578/ http://bdigital.unal.edu.co/21578/2/ |
url |
https://repositorio.unal.edu.co/handle/unal/31499 http://bdigital.unal.edu.co/21578/ http://bdigital.unal.edu.co/21578/2/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/agrocol/article/view/18065 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
dc.relation.ispartofseries.none.fl_str_mv |
Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965 |
dc.relation.references.spa.fl_str_mv |
Guzmàn, Mònica and Rodriguez, Patricia (2010) Susceptibility of solanum phureja (juz. et buk.) to potato yellow vein virus. Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 Agronomía Colombiana; Vol. 28, núm. 2 (2010); 219-224 2357-3732 0120-9965 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
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