Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability

Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However, maintain...

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Autores:
Henao Duque, Ana María
Núñez Rangel, Vitelbina
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/61170
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/61170
http://bdigital.unal.edu.co/59978/
Palabra clave:
57 Ciencias de la vida; Biología / Life sciences; biology
antivenom
force-feeding
mortality.
alimentación forzada
antiveneno
mortalidad.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_2bfce1832668a8cb9a04d12b09f6e03c
oai_identifier_str oai:repositorio.unal.edu.co:unal/61170
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
spellingShingle Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
57 Ciencias de la vida; Biología / Life sciences; biology
antivenom
force-feeding
mortality.
alimentación forzada
antiveneno
mortalidad.
title_short Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_full Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_fullStr Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_full_unstemmed Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_sort Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
dc.creator.fl_str_mv Henao Duque, Ana María
Núñez Rangel, Vitelbina
dc.contributor.author.spa.fl_str_mv Henao Duque, Ana María
Núñez Rangel, Vitelbina
dc.subject.ddc.spa.fl_str_mv 57 Ciencias de la vida; Biología / Life sciences; biology
topic 57 Ciencias de la vida; Biología / Life sciences; biology
antivenom
force-feeding
mortality.
alimentación forzada
antiveneno
mortalidad.
dc.subject.proposal.spa.fl_str_mv antivenom
force-feeding
mortality.
alimentación forzada
antiveneno
mortalidad.
description Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However, maintaining this species in captivity is very difficult because it refuses to feed, in addition to the high mortality rate due to maladaptation syndrome. In this study a force feeding diet, diverse substrates for maintenance and a milking technique were evaluated. Additionally, individual variability of the venom was determined by High Performance Liquid Chromatography (HPLC), Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coagulant, Anticoagulant and Hemolytic activities. The results of this study demonstrate that it was possible to increase the survival rate of this species in captivity and to determine some of the important factors in the maintenance. As to the individual variability of the venom, we found differences in number and intensity of peaks recovered by chromatography and also displayed variations in some of its biological activities.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-09-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T20:06:18Z
dc.date.available.spa.fl_str_mv 2019-07-02T20:06:18Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica Colombiana
Acta Biológica Colombiana
dc.relation.references.spa.fl_str_mv Henao Duque, Ana María and Núñez Rangel, Vitelbina (2016) Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability. Acta Biológica Colombiana, 21 (3). pp. 593-600. ISSN 1900-1649
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
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http://creativecommons.org/licenses/by-nc/4.0/
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eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biología
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Henao Duque, Ana María7a6589e3-a7dd-4e82-a907-a1476dcc1a93300Núñez Rangel, Vitelbina281a101c-d8bf-4497-b506-d1a96e1e5f793002019-07-02T20:06:18Z2019-07-02T20:06:18Z2016-09-01ISSN: 1900-1649https://repositorio.unal.edu.co/handle/unal/61170http://bdigital.unal.edu.co/59978/Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However, maintaining this species in captivity is very difficult because it refuses to feed, in addition to the high mortality rate due to maladaptation syndrome. In this study a force feeding diet, diverse substrates for maintenance and a milking technique were evaluated. Additionally, individual variability of the venom was determined by High Performance Liquid Chromatography (HPLC), Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coagulant, Anticoagulant and Hemolytic activities. The results of this study demonstrate that it was possible to increase the survival rate of this species in captivity and to determine some of the important factors in the maintenance. As to the individual variability of the venom, we found differences in number and intensity of peaks recovered by chromatography and also displayed variations in some of its biological activities.La coral “rabo de ají” es una coral bicolor larga y delgada. Esta especie está ampliamente distribuida en Colombia y es la coral que causa el mayor número de accidentes en la región Andina, por esto es importante mantener esta especie en cautiverio con fines de producción de antivenenos e investigación. No obstante, el mantenimiento de esta especie en cautiverio es difícil, debido a que se rehúsan a alimentarse voluntariamente y a que presentan alta mortalidad por el denominado síndrome de mal adaptación. En este estudio se evaluaron varios  sustratos para el mantenimiento, además de una dieta forzada y una técnica de ordeño. Adicionalmente, se evaluó la variabilidad individual del veneno a través de cromatografía liquida de alta eficiencia (HPLC), electroforesis (SDS-PAGE) y las actividades coagulante, anticoagulante y hemolítica indirecta. Los resultados de este estudio demostraron que fue posible incrementar la sobrevivencia de esta especie en cautiverio, así como determinar algunos factores importantes en su mantenimiento. A partir de la evaluación del veneno se encontraron diferencias en el número y en la intensidad de picos en la cromatografía, así como en algunas de sus actividades biológicas.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biologíahttps://revistas.unal.edu.co/index.php/actabiol/article/view/51651Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica ColombianaActa Biológica ColombianaHenao Duque, Ana María and Núñez Rangel, Vitelbina (2016) Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability. Acta Biológica Colombiana, 21 (3). pp. 593-600. ISSN 1900-164957 Ciencias de la vida; Biología / Life sciences; biologyantivenomforce-feedingmortality.alimentación forzadaantivenenomortalidad.Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variabilityArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL51651-303537-1-PB.pdfapplication/pdf318533https://repositorio.unal.edu.co/bitstream/unal/61170/1/51651-303537-1-PB.pdfb79a9d9fb71693890c5020805391a1adMD51THUMBNAIL51651-303537-1-PB.pdf.jpg51651-303537-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8982https://repositorio.unal.edu.co/bitstream/unal/61170/2/51651-303537-1-PB.pdf.jpg976c4a62cebc280de3091d001f780d24MD52unal/61170oai:repositorio.unal.edu.co:unal/611702023-04-10 23:05:46.831Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co