Photocatalytic disinfection treatments: viability, cultivability and metabolic changes of e. coli using diferent mesurements methods

In this work, the oxidative stress was produced by the illumination of either fixed TiO₂ or suspended TiO₂, and the relation between cultivability and viability under oxidative conditions was studied using a Direct Viable Count (DVC) and Fluorescence In Situ Hybridisation (FISH) methods. Under certa...

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Autores:
Sanabria Gómez, Irma Janeth
Wist, Julien
Pulgarín Gómez, César
Tipo de recurso:
Article of journal
Fecha de publicación:
2011
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/37748
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/37748
http://bdigital.unal.edu.co/27832/
Palabra clave:
Photocatalysis
drinking water
E. coli
oxidative stress
titanium dioxide
cultivability
disinfection
NMR
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
Description
Summary:In this work, the oxidative stress was produced by the illumination of either fixed TiO₂ or suspended TiO₂, and the relation between cultivability and viability under oxidative conditions was studied using a Direct Viable Count (DVC) and Fluorescence In Situ Hybridisation (FISH) methods. Under certain oxidative conditions simulating incomplete treatments, viable cells that had lost their cultivability were observed to recover all of their physiological functions when transferred to fresh nutrient media. Moreover, alterations in the nutrient assimilation processes were studied by 1H-NMR during and after the photocatalytic treatment. This allowed us to shed some light on how the damage chain propagates during the treatment. This work demonstrate that the Standard Plate Count (SPC) method commonly used to estimate the c ell populations during disinfection processes is ill adapted, whereas the FISH-DVC pair offers a reliable alternative.