Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp
The Colombian Creole Cattle has showed a disturbing decrease in population, from 23,415 individuals in 1999 to 20,102 in 2003. Despite that many efforts to recover the creole breeds have been done, its future conservation is unclear. Searching for economic desirable genes may contribute to its pres...
- Autores:
-
Rosero-Alpala, Jaime A.
Alvarez Franco, Luz Angela
Muñoz Florez, Jaime Eduardo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2011
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/39997
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/39997
http://bdigital.unal.edu.co/30094/
- Palabra clave:
- 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
a-LA
b-LG
bovinos criollos
Colombia
globulina
proteínas del suero
α-LA
β-LG
Colombia
creole cattle
globulins
whey proteins
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp |
| title |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp |
| spellingShingle |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp 6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture a-LA b-LG bovinos criollos Colombia globulina proteínas del suero α-LA β-LG Colombia creole cattle globulins whey proteins |
| title_short |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp |
| title_full |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp |
| title_fullStr |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp |
| title_full_unstemmed |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp |
| title_sort |
Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp |
| dc.creator.fl_str_mv |
Rosero-Alpala, Jaime A. Alvarez Franco, Luz Angela Muñoz Florez, Jaime Eduardo |
| dc.contributor.author.spa.fl_str_mv |
Rosero-Alpala, Jaime A. Alvarez Franco, Luz Angela Muñoz Florez, Jaime Eduardo |
| dc.subject.ddc.spa.fl_str_mv |
6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture |
| topic |
6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture a-LA b-LG bovinos criollos Colombia globulina proteínas del suero α-LA β-LG Colombia creole cattle globulins whey proteins |
| dc.subject.proposal.spa.fl_str_mv |
a-LA b-LG bovinos criollos Colombia globulina proteínas del suero α-LA β-LG Colombia creole cattle globulins whey proteins |
| description |
The Colombian Creole Cattle has showed a disturbing decrease in population, from 23,415 individuals in 1999 to 20,102 in 2003. Despite that many efforts to recover the creole breeds have been done, its future conservation is unclear. Searching for economic desirable genes may contribute to its preservation and utilization as a genetic resource. Genes related with the improvement of milk proteins are considered as an economic important factor by the dairy industry. With the aim of characterizing beta-lactoglobulin (β-LG) and alpha-lactalbumin (α-LA) genes, 30 samples from each of the creole breeds (Blanco Orejinegro, Caqueteño, Casanareño, Costeño Con Cuernos, Chino Santandereano, Hartón del Valle, Romosinuano and Sanmartinero), two Colombian breeds (Lucerna and Velásquez) and two introduced breeds (Holstein and Brahman) were analyzed. A DNA fragment of 262 bp for β-LG and 166 for α-LA using PCR-SSCP were amplified and analyzed. The average frequencies for β-LG (A) and β-LG (B) were 0.46 ± 0.020 and 0.53 ± 0.020, respectively, and 0.35 ± 0.019 for α-LA (A) and 0.64 ± 0.019 for α-LA (B). The genetic diversity (He) average for β-LG was 0.498 and 0.455 for α-LA. Creole breeds represent a valuable genetic base as an alternative for breeding and improvement programs in dairy production herds in order to produce milk with desirable characteristics for the dairy industry. |
| publishDate |
2011 |
| dc.date.issued.spa.fl_str_mv |
2011-10-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-28T09:27:44Z |
| dc.date.available.spa.fl_str_mv |
2019-06-28T09:27:44Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/redcol/resource_type/ART |
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ISSN: 2323-0118 |
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https://repositorio.unal.edu.co/handle/unal/39997 |
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http://bdigital.unal.edu.co/30094/ |
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ISSN: 2323-0118 |
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spa |
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spa |
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http://revistas.unal.edu.co/index.php/acta_agronomica/article/view/28850 |
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Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica Acta Agronómica |
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Rosero-Alpala, Jaime A. and Alvarez Franco, Luz Angela and Muñoz Florez, Jaime Eduardo (2011) Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp. Acta Agronómica, 60 (4). pp. 339-346. ISSN 2323-0118 |
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Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Universidad Nacional de Colombia Sede Palmira |
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Universidad Nacional de Colombia |
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https://repositorio.unal.edu.co/bitstream/unal/39997/1/28850-103449-2-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/39997/2/28850-103449-2-PB.pdf.jpg |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rosero-Alpala, Jaime A.e6b05b0f-cf9a-4ea1-905a-94460bd70a6d300Alvarez Franco, Luz Angela39b2f2d6-ff5e-4356-a052-eb97b4832551300Muñoz Florez, Jaime Eduardode00a3e9-9ff7-4c8e-bcae-d5699ea4c1d43002019-06-28T09:27:44Z2019-06-28T09:27:44Z2011-10-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/39997http://bdigital.unal.edu.co/30094/The Colombian Creole Cattle has showed a disturbing decrease in population, from 23,415 individuals in 1999 to 20,102 in 2003. Despite that many efforts to recover the creole breeds have been done, its future conservation is unclear. Searching for economic desirable genes may contribute to its preservation and utilization as a genetic resource. Genes related with the improvement of milk proteins are considered as an economic important factor by the dairy industry. With the aim of characterizing beta-lactoglobulin (β-LG) and alpha-lactalbumin (α-LA) genes, 30 samples from each of the creole breeds (Blanco Orejinegro, Caqueteño, Casanareño, Costeño Con Cuernos, Chino Santandereano, Hartón del Valle, Romosinuano and Sanmartinero), two Colombian breeds (Lucerna and Velásquez) and two introduced breeds (Holstein and Brahman) were analyzed. A DNA fragment of 262 bp for β-LG and 166 for α-LA using PCR-SSCP were amplified and analyzed. The average frequencies for β-LG (A) and β-LG (B) were 0.46 ± 0.020 and 0.53 ± 0.020, respectively, and 0.35 ± 0.019 for α-LA (A) and 0.64 ± 0.019 for α-LA (B). The genetic diversity (He) average for β-LG was 0.498 and 0.455 for α-LA. Creole breeds represent a valuable genetic base as an alternative for breeding and improvement programs in dairy production herds in order to produce milk with desirable characteristics for the dairy industry.La población de ganado criollo colombiano ha venido presentando una inquietante disminución al pasar de 23.415 ejemplares en 1999 a 20.102 en 2003. A pesar de los esfuerzos por recuperar las razas criollas el panorama para su conservación es incierto, por tanto la búsqueda de caracteres deseables puede contribuir a su valoración y conservación. Los genes relacionados con el mejoramiento de la calidad de la leche producida por estas razas se consideran de gran importancia en la industria láctea, por tal razón y con el objetivo de caracterizar los genes beta-lactoglobulina y alpha-lactoalbúmina se analizaron 30 muestras de sangre de cada una de las razas criollas (Blanco Orejinegro, Caqueteño, Casanareño, Costeño con cuernos, Chino Santandereano, Hartón del Valle, Romosinuano y Sanmartinero), dos razas sintéticas colombianas (Lucerna y Velásquez) y dos razas foráneas (Holstein y Brahman). Se amplificaron fragmentos de 262pb para beta-lactoglobulina (b-LG) y de 166 pb para alpha-lactoalbúmina (a-LA) que se genotipificaron mediante PCR-SSCP. El promedio de la frecuencia para b-LG A y b-LG B fue de 0.46 ± 0.020 y de 0.53 ± 0.020, respectivamente, y de 0.35 ± 0.019 para a-LA A y 0.64 ± 0.019 para a-LA B. El promedio de diversidad genética (He) para b-LG fue 0.498 y de 0.455 para a-LA. Los ganados criollos representan una base genética valiosa, como alternativa para mejorar genéticamente los hatos destinados a la producción de leche con mejores características en calidad para la industria láctea.application/pdfspaUniversidad Nacional de Colombia Sede Palmirahttp://revistas.unal.edu.co/index.php/acta_agronomica/article/view/28850Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaRosero-Alpala, Jaime A. and Alvarez Franco, Luz Angela and Muñoz Florez, Jaime Eduardo (2011) Genetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscp. Acta Agronómica, 60 (4). pp. 339-346. ISSN 2323-01186 Tecnología (ciencias aplicadas) / Technology63 Agricultura y tecnologías relacionadas / Agriculturea-LAb-LGbovinos criollosColombiaglobulinaproteínas del sueroα-LAβ-LGColombiacreole cattleglobulinswhey proteinsGenetic polymorphism of beta-lactoglobulin and alpha-lactoalbumin in colombian creole cattle by pcr-sscpArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL28850-103449-2-PB.pdfapplication/pdf321004https://repositorio.unal.edu.co/bitstream/unal/39997/1/28850-103449-2-PB.pdfc85d731057b2fb3dbe0244b0eebda34fMD51THUMBNAIL28850-103449-2-PB.pdf.jpg28850-103449-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg8399https://repositorio.unal.edu.co/bitstream/unal/39997/2/28850-103449-2-PB.pdf.jpg834d9c60e6b2e4170730103b9166446fMD52unal/39997oai:repositorio.unal.edu.co:unal/399972024-01-22 23:06:54.88Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |