Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.

ilustraciones, gráficas, tablas

Autores:: Fonseca Fernández, Angie Lorena

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.
dc.title.translated.eng.fl_str_mv	Molecular and in vitro sensitivity characterization to antibiotics and cathelicidin peptides as antimicrobial agents against clinical isolates of Staphylococcus aureus from Bogotá city.
title	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.
spellingShingle	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C. 610 - Medicina y salud::616 - Enfermedades Programas de Optimización del Uso de los Antimicrobianos Aislamiento de Pacientes Antimicrobial Stewardship Patient Isolation Staphylococcus aureus Péptidos antimicrobianos Catelicidina Resistencia antibiótica Resistencia a meticilina LL-37 ATRA-1 Antimicrobial peptides Antibiotic resistance Cathelicidin Methicillin resistance
title_short	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.
title_full	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.
title_fullStr	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.
title_full_unstemmed	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.
title_sort	Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.
dc.creator.fl_str_mv	Fonseca Fernández, Angie Lorena
dc.contributor.advisor.none.fl_str_mv	Celis Ramírez, Adriana Marcela Leal Castro, Aura Lucía
dc.contributor.author.none.fl_str_mv	Fonseca Fernández, Angie Lorena
dc.contributor.researcher.none.fl_str_mv	Mancera García, María Alejandra Martínez, Heydys Chad, Leidy Guevara-Suárez, Marcela
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación Celular y Molecular de Microorganismos Patógenos
dc.subject.ddc.spa.fl_str_mv	610 - Medicina y salud::616 - Enfermedades
topic	610 - Medicina y salud::616 - Enfermedades Programas de Optimización del Uso de los Antimicrobianos Aislamiento de Pacientes Antimicrobial Stewardship Patient Isolation Staphylococcus aureus Péptidos antimicrobianos Catelicidina Resistencia antibiótica Resistencia a meticilina LL-37 ATRA-1 Antimicrobial peptides Antibiotic resistance Cathelicidin Methicillin resistance
dc.subject.decs.spa.fl_str_mv	Programas de Optimización del Uso de los Antimicrobianos Aislamiento de Pacientes
dc.subject.decs.eng.fl_str_mv	Antimicrobial Stewardship Patient Isolation
dc.subject.proposal.spa.fl_str_mv	Staphylococcus aureus Péptidos antimicrobianos Catelicidina Resistencia antibiótica Resistencia a meticilina
dc.subject.proposal.eng.fl_str_mv	LL-37 ATRA-1 Antimicrobial peptides Antibiotic resistance Cathelicidin Methicillin resistance
description	ilustraciones, gráficas, tablas
publishDate	2022
dc.date.issued.none.fl_str_mv	2022
dc.date.accessioned.none.fl_str_mv	2023-01-16T16:23:10Z
dc.date.available.none.fl_str_mv	2023-01-16T16:23:10Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/82941
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/82941 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.rights.spa.fl_str_mv	Derechos reservados al autor, 2022
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dc.rights.license.spa.fl_str_mv	Reconocimiento 4.0 Internacional
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rights_invalid_str_mv	Reconocimiento 4.0 Internacional Derechos reservados al autor, 2022 http://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2
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dc.format.extent.spa.fl_str_mv	62 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Reconocimiento 4.0 InternacionalDerechos reservados al autor, 2022http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Celis Ramírez, Adriana Marcelaed423c7385d34e43cde87e44089f0390Leal Castro, Aura Lucíab5b5a72c792fefdbe852bd28474b6008Fonseca Fernández, Angie Lorena9be27f4bcde11008dea21e50aa5467ccMancera García, María AlejandraMartínez, HeydysChad, LeidyGuevara-Suárez, MarcelaGrupo de Investigación Celular y Molecular de Microorganismos Patógenos2023-01-16T16:23:10Z2023-01-16T16:23:10Z2022https://repositorio.unal.edu.co/handle/unal/82941Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficas, tablasStaphylococcus aureus es una bacteria de gran importancia clínica, caracterizada por ser la especie más virulenta dentro de su género y causante de infecciones muy diversas que pueden amenazar la vida de quienes las padecen. Sin embargo, su importancia no radica únicamente en las enfermedades que causa, sino en la alta capacidad que ha evidenciado para presentar resistencia a múltiples antibióticos dentro de los que se encuentran los βlactámicos, ejemplificada por la resistencia a meticilina. Los aislamientos resistentes a meticilina son un problema de salud pública mundial, que inicialmente se atribuían a ambientes hospitalarios, pero que con el paso del tiempo han comenzado a reportarse cada vez con más frecuencia en la comunidad. Adicional a esto, se ha observado resistencia a otros antibióticos con blancos terapéuticos diferentes. Lo anterior hace necesario la búsqueda de nuevas alternativas que permitan controlar el crecimiento bacteriano de aislamientos que no pueden tratarse mediante los antimicrobianos existentes actualmente. Este trabajo tiene como objetivo determinar el efecto de péptidos derivados de catelicidina en el crecimiento y la morfología de aislamientos clínicos de S. aureus procedentes de la ciudad de Bogotá D.C. Para ello, realizaremos la caracterización molecular respecto al gen de resistencia a meticilina mecA, la proteína A (spa) y pvl , causante de la presencia de leucocidina Panton-Valentine, e identificaremos los perfiles de sensibilidad a compuestos antibióticos de 57 aislamientos clínicos de S. aureus. Adicionalmente, evaluaremos la actividad de péptidos de catelicidina, LL-37 y ATRA-1, y daptomicina sobre los aislamientos clínicos caracterizados. Finalmente, identificaremos los cambios en la morfología bacteriana de S. aureus, por acción de los péptidos de catelicidina, mediante microscopía electrónica de barrido. Determinamos que existe una importante prevalencia de aislamientos resistentes a meticilina, y que además, existen aislamientos con discordancias en el perfil de sensibilidad a estos antibióticos en S. aureus, por otra parte se evidencia una tasa alta de sensibilidad a antibióticos con mecanismos de acciones diferentes a meticilina. Comprobamos que los péptidos LL-37 y ATRA-1 presentan capacidad antimicrobiana frente a este patógeno y que existen diferencias entre la actividad de estos compuestos y el perfil de sensibilidad a meticiilina. Finalmente, comprobamos que estos péptidos generan cambios en la morfología superficial como uno de sus mecanismos de acción, sin embargo, no se descarta que presenten efectos intracelulares que potencien su actividad antimicrobiana. Con este trabajo, buscamos contribuir a la vigilancia de la resistencia bacteriana en la ciudad de Bogotá, promover el desarrollo de nuevas alternativas terapéuticas que permitan controlar la resistencia bacteriana en nuestra región, así como aportar en la caracterización de la actividad de péptidos antimicrobianos sintéticos en S. aureus. (Texto tomado de la fuente)Staphylococcus aureus is a bacterium of great clinical importance. It is the most virulent species within its genus and the cause of very diverse infections that can threaten the lives of those who suffer from them. More importantly, it has shown resistance to multiple antibiotics, including β-lactam antibiotics, like Methicillin resistance. Methicillin-resistant isolates are a worldwide public health problem, attributed initially to hospital environments, but it has begun to be reported more and more frequently in the community. In addition to this, resistance to other antibiotics with different therapeutic targets has been observed. That makes it necessary to search for new alternatives to control the bacterial growth of isolates that cannot be treated with existing antibiotics. This work aims to determine the effect of cathelicidin-derived peptides on the growth and morphology of S. aureus clinical isolates from Bogotá D.C. city. We performed molecular characterization of the methicillin resistance mecA gene, protein A (spa), and pvl genes, and we identified the sensitivity profiles to the antibiotics of 57 S. aureus clinical isolates. Additionally, we evaluated the antimicrobial activity of LL-37, ATRA-1, and daptomycin on the characterized clinical isolates. Finally, we identified the changes in S. aureus bacterial morphology by peptides’ action using scanning electron microscopy (SEM). We determined that there is an important prevalence of resistant isolates to Methicillin. Some of them with discordances in the methicillin sensitivity in S. aureus. However, a high sensitivity rate to antibiotics with mechanisms of action different from methicillin is evidenced. We found that LL-37 and ATRA-1 peptides have antimicrobial capacity against this pathogen and are differences between the activity of these compounds and the sensitivity of the Methicillin profile. Finally, we proved that these peptides generate Surface changes in morphology as one of their mechanisms; however, it is not excluded that they present intracellular effects that potentiate its antimicrobial action. With this work, we are looking to contribute to the surveillance of bacterial resistance in Bogotá city, promote the development of new therapeutic strategies to control bacterial resistance in our region, and contribute to the characterization of the activity of synthetic antimicrobial peptides against S. aureus.MaestríaMagíster en Ciencias - MicrobiologíaCaracterización de aislamientos de S. aureus Perfil de sensibilidad antibiótica En este estudio se incluyeron 57 aislamientos proporcionados por el Grupo para el Control de la resistencia bacteriana en Bogotá (GREBO) y el laboratorio de Biofísica de la Universidad de los Andes (ver Anexo A). A partir de estos aislamientos se realizaron cultivos iniciales de 24 h a 37°C en medio solido tripticasa de soya (TSA) (Scharlau Chemie, España). La sensibilidad oxacilina se evalúo empleando el método microdilución en caldo conforme a lo descrito en la norma CLSI M07 (Clinical and Laboratory Standards Institute, 2018) y a cefoxitina, gentamicina, eritromicina, clindamicina, ciprofloxacina y trimetoprima-sulfametoxazol empleando el método difusión en agar conforme a lo descrito en la normar CLSI M02 (Clinical and Laboratory Standards Institute, 2018). Como controles se emplearon las cepas Staphylococcus aureus subsp. aureus (ATCC® 25923™), Staphylococcus aureus subsp. aureus (ATCC® 29213™) y Staphylococcus aureus subsp. aureus (ATCC® 33591™) (American Type Culture Collection, Virginia, USA) y los aislamientos clínicos 1631 sensible a daptomicina y 1634 resistente a daptomicina, proporcionados por el laboratorio de Biofísica de la Universidad de los Andes (ver anexo A). Todos los ensayos se realizaron por triplicado.Microbiología médicaBiología molecular de agentes infecciosos62 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::616 - EnfermedadesProgramas de Optimización del Uso de los AntimicrobianosAislamiento de PacientesAntimicrobial StewardshipPatient IsolationStaphylococcus aureusPéptidos antimicrobianosCatelicidinaResistencia antibióticaResistencia a meticilinaLL-37ATRA-1Antimicrobial peptidesAntibiotic resistanceCathelicidinMethicillin resistanceCaracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.Molecular and in vitro sensitivity characterization to antibiotics and cathelicidin peptides as antimicrobial agents against clinical isolates of Staphylococcus aureus from Bogotá city.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbente, S., Carpinelli, L., Guillén, R., Rodríguez, F., Fariña, N., Laspina, F., & López, Y. 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Biological and Pharmaceutical Bulletin, b20-00217.Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.MinCienciasEstudiantesGrupos comunitariosInvestigadoresMaestrosMedios de comunicaciónPadres y familiasPersonal de apoyo escolarProveedores de ayuda financiera para estudiantesPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/82941/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53ORIGINAL1013675857.2022.pdf1013675857.2022.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf1358519https://repositorio.unal.edu.co/bitstream/unal/82941/4/1013675857.2022.pdf014f2cff27cbd6c43b72e9bf2755a3b7MD54THUMBNAIL1013675857.2022.pdf.jpg1013675857.2022.pdf.jpgGenerated 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WwgdHJhdGFtaWVudG8gZGUgZGF0b3MgcGVyc29uYWxlcywgZGUgYWN1ZXJkbyBjb24gbGEgbGV5IDE1ODEgZGUgMjAxMiBlbnRlbmRpZW5kbyBxdWUgc2UgZW5jdWVudHJhbiBiYWpvIG1lZGlkYXMgcXVlIGdhcmFudGl6YW4gbGEgc2VndXJpZGFkLCBjb25maWRlbmNpYWxpZGFkIGUgaW50ZWdyaWRhZCwgeSBzdSB0cmF0YW1pZW50byB0aWVuZSB1bmEgZmluYWxpZGFkIGhpc3TDs3JpY2EsIGVzdGFkw61zdGljYSBvIGNpZW50w61maWNhIHNlZ8O6biBsbyBkaXNwdWVzdG8gZW4gbGEgUG9sw610aWNhIGRlIFRyYXRhbWllbnRvIGRlIERhdG9zIFBlcnNvbmFsZXMuCgoKClBBUlRFIDIuIEFVVE9SSVpBQ0nDk04gUEFSQSBQVUJMSUNBUiBZIFBFUk1JVElSIExBIENPTlNVTFRBIFkgVVNPIERFIE9CUkFTIEVOIEVMIFJFUE9TSVRPUklPIElOU1RJVFVDSU9OQUwgVU5BTC4KClNlIGF1dG9yaXphIGxhIHB1YmxpY2FjacOzbiBlbGVjdHLDs25pY2EsIGNvbnN1bHRhIHkgdXNvIGRlIGxhIG9icmEgcG9yIHBhcnRlIGRlIGxhIFVuaXZlcnNpZGFkIE5hY2lvbmFsIGRlIENvbG9tYmlhIHkgZGUgc3VzIHVzdWFyaW9zIGRlIGxhIHNpZ3VpZW50ZSBtYW5lcmE6CgphLglDb25jZWRvIGxpY2VuY2lhIGVuIGxvcyB0w6lybWlub3Mgc2XDsWFsYWRvcyBlbiBsYSBwYXJ0ZSAxIGRlbCBwcmVzZW50ZSBkb2N1bWVudG8sIGNvbiBlbCBvYmpldGl2byBkZSBxdWUgbGEgb2JyYSBlbnRyZWdhZGEgc2VhIHB1YmxpY2FkYSBlbiBlbCBSZXBvc2l0b3JpbyBJbnN0aXR1Y2lvbmFsIGRlIGxhIFVuaXZlcnNpZGFkIE5hY2lvbmFsIGRlIENvbG9tYmlhIHkgcHVlc3RhIGEgZGlzcG9zaWNpw7NuIGVuIGFjY2VzbyBhYmllcnRvIHBhcmEgc3UgY29uc3VsdGEgcG9yIGxvcyB1c3VhcmlvcyBkZSBsYSBVbml2ZXJzaWRhZCBOYWNpb25hbCBkZSBDb2xvbWJpYSAgYSB0cmF2w6lzIGRlIGludGVybmV0LgoKCgpQQVJURSAzIEFVVE9SSVpBQ0nDk04gREUgVFJBVEFNSUVOVE8gREUgREFUT1MgUEVSU09OQUxFUy4KCkxhIFVuaXZlcnNpZGFkIE5hY2lvbmFsIGRlIENvbG9tYmlhLCBjb21vIHJlc3BvbnNhYmxlIGRlbCBUcmF0YW1pZW50byBkZSBEYXRvcyBQZXJzb25hbGVzLCBpbmZvcm1hIHF1ZSBsb3MgZGF0b3MgZGUgY2Fyw6FjdGVyIHBlcnNvbmFsIHJlY29sZWN0YWRvcyBtZWRpYW50ZSBlc3RlIGZvcm11bGFyaW8sIHNlIGVuY3VlbnRyYW4gYmFqbyBtZWRpZGFzIHF1ZSBnYXJhbnRpemFuIGxhIHNlZ3VyaWRhZCwgY29uZmlkZW5jaWFsaWRhZCBlIGludGVncmlkYWQgeSBzdSB0cmF0YW1pZW50byBzZSByZWFsaXphIGRlIGFjdWVyZG8gYWwgY3VtcGxpbWllbnRvIG5vcm1hdGl2byBkZSBsYSBMZXkgMTU4MSBkZSAyMDEyIHkgZGUgbGEgUG9sw610aWNhIGRlIFRyYXRhbWllbnRvIGRlIERhdG9zIFBlcnNvbmFsZXMgZGUgbGEgVW5pdmVyc2lkYWQgTmFjaW9uYWwgZGUgQ29sb21iaWEuIFB1ZWRlIGVqZXJjZXIgc3VzIGRlcmVjaG9zIGNvbW8gdGl0dWxhciBhIGNvbm9jZXIsIGFjdHVhbGl6YXIsIHJlY3RpZmljYXIgeSByZXZvY2FyIGxhcyBhdXRvcml6YWNpb25lcyBkYWRhcyBhIGxhcyBmaW5hbGlkYWRlcyBhcGxpY2FibGVzIGEgdHJhdsOpcyBkZSBsb3MgY2FuYWxlcyBkaXNwdWVzdG9zIHkgZGlzcG9uaWJsZXMgZW4gd3d3LnVuYWwuZWR1LmNvIG8gZS1tYWlsOiBwcm90ZWNkYXRvc19uYUB1bmFsLmVkdS5jbyIKClRlbmllbmRvIGVuIGN1ZW50YSBsbyBhbnRlcmlvciwgYXV0b3Jpem8gZGUgbWFuZXJhIHZvbHVudGFyaWEsIHByZXZpYSwgZXhwbMOtY2l0YSwgaW5mb3JtYWRhIGUgaW5lcXXDrXZvY2EgYSBsYSBVbml2ZXJzaWRhZCBOYWNpb25hbCBkZSBDb2xvbWJpYSBhIHRyYXRhciBsb3MgZGF0b3MgcGVyc29uYWxlcyBkZSBhY3VlcmRvIGNvbiBsYXMgZmluYWxpZGFkZXMgZXNwZWPDrWZpY2FzIHBhcmEgZWwgZGVzYXJyb2xsbyB5IGVqZXJjaWNpbyBkZSBsYXMgZnVuY2lvbmVzIG1pc2lvbmFsZXMgZGUgZG9jZW5jaWEsIGludmVzdGlnYWNpw7NuIHkgZXh0ZW5zacOzbiwgYXPDrSBjb21vIGxhcyByZWxhY2lvbmVzIGFjYWTDqW1pY2FzLCBsYWJvcmFsZXMsIGNvbnRyYWN0dWFsZXMgeSB0b2RhcyBsYXMgZGVtw6FzIHJlbGFjaW9uYWRhcyBjb24gZWwgb2JqZXRvIHNvY2lhbCBkZSBsYSBVbml2ZXJzaWRhZC4gCgo=

Caracterización molecular y de sensibilidad in vitro a antibióticos y péptidos derivados de catelicidina como agentes antimicrobianos frente a aislamientos clínicos de Staphylococcus aureus procedentes de la ciudad de Bogotá D.C.

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