Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano

La resistencia a los antimicrobianos (RAM) es una de las diez principales amenazas para la salud pública reportadas por la Organización Mundial de la Salud (OMS). Una de las causas del creciente problema de la RAM es la falta de nuevas terapias y/o agentes de tratamiento; en consecuencia, muchas enf...

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Autores:: Pineda Castañeda, Héctor Manuel

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano
dc.title.translated.eng.fl_str_mv	Peptide-resorcinarene dendrimers: (i) obtaining by azide/alkyne cycloaddition reaction and (ii) evaluation of their antibacterial potential
title	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano
spellingShingle	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano 540 - Química y ciencias afines Programas de Optimización del Uso de los Antimicrobianos Antiinfecciosos Antimicrobial Stewardship Anti-Infective Agents Dendrimeros Péptido Resorcinareno Péptidos Antimicrobianos Química click
title_short	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano
title_full	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano
title_fullStr	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano
title_full_unstemmed	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano
title_sort	Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano
dc.creator.fl_str_mv	Pineda Castañeda, Héctor Manuel
dc.contributor.advisor.none.fl_str_mv	Maldona Villamil, Mauricio Rivera Monroy, Zuly Jenny
dc.contributor.author.none.fl_str_mv	Pineda Castañeda, Héctor Manuel
dc.contributor.researchgroup.spa.fl_str_mv	Síntesis y Aplicación de Moléculas Peptídicas Aplicaciones Analíticas de Compuestos Orgánicos (Aaco)
dc.contributor.orcid.spa.fl_str_mv	0000-0002-9081-0546
dc.contributor.cvlac.spa.fl_str_mv	https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000073189
dc.contributor.scopus.spa.fl_str_mv	https://www.scopus.com/authid/detail.uri?authorId=57208139842
dc.contributor.researchgate.spa.fl_str_mv	https://www.researchgate.net/profile/Hector-Pineda-Castaneda
dc.contributor.googlescholar.spa.fl_str_mv	https://scholar.google.com/citations?user=Wu60Xw0AAAAJ&hl=es&authuser=3
dc.subject.ddc.spa.fl_str_mv	540 - Química y ciencias afines
topic	540 - Química y ciencias afines Programas de Optimización del Uso de los Antimicrobianos Antiinfecciosos Antimicrobial Stewardship Anti-Infective Agents Dendrimeros Péptido Resorcinareno Péptidos Antimicrobianos Química click
dc.subject.decs.spa.fl_str_mv	Programas de Optimización del Uso de los Antimicrobianos Antiinfecciosos
dc.subject.decs.eng.fl_str_mv	Antimicrobial Stewardship Anti-Infective Agents
dc.subject.proposal.spa.fl_str_mv	Dendrimeros Péptido Resorcinareno Péptidos Antimicrobianos Química click
description	La resistencia a los antimicrobianos (RAM) es una de las diez principales amenazas para la salud pública reportadas por la Organización Mundial de la Salud (OMS). Una de las causas del creciente problema de la RAM es la falta de nuevas terapias y/o agentes de tratamiento; en consecuencia, muchas enfermedades infecciosas podrían volverse incontrolables. La necesidad de descubrir nuevos agentes antimicrobianos, que sean alternativos a los existentes, y que permitan mitigar este problema, se ha incrementado debido a la rápida y global expansión de la RAM. En este contexto, se han propuesto como alternativas para combatir la RAM tanto los péptidos antimicrobianos (PAMs) como los dendrímeros que presentan múltiples copias de compuestos antibacterianos en su estructura. Los dendrímeros han exhibido propiedades antifúngicas y antibacterianas y también se han utilizado en terapias antiinflamatorias, antineoplásicas y cardiovasculares y son útiles en sistemas de administración de fármacos y genes. En este trabajo se propuso obtener dendrímeros que presenten cuatro copias de secuencias de PAMs. Específicamente, (i) se exploró la síntesis, purificación y caracterización de dendrímeros de péptido-resorcinareno derivados de las secuencias LfcinB (20-25): RRWQWR y BF (32-35): RLLR, y (ii) la actividad antimicrobiana y citotóxica de estos dendrímeros. Se establecieron las rutas de síntesis que permitieron obtener: a) alquino-resorcinarenos y b) péptidos funcionalizados con el grupo azida; los cuales se usaron para generar c) dendrímeros de péptido-resorcinareno mediante química click de cicloadición de azida-alquino CuAAC. Finalmente, se evaluó la actividad antimicrobiana y citotóxica de los dendrímeros obtenidos frente a cepas de referencia y aislados clínicos. Estos resultados permitieron la identificación de moléculas antimicrobianas prometedoras que pueden conducir a avances en el desarrollo de nuevos agentes terapéuticos. (Texto tomado de la fuente)
publishDate	2023
dc.date.issued.none.fl_str_mv	2023
dc.date.accessioned.none.fl_str_mv	2024-02-07T13:53:46Z
dc.date.available.none.fl_str_mv	2024-02-07T13:53:46Z
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	DataPaper
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/85642
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/85642 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Maldona Villamil, Mauricio22af21f285c3f5f3c3e580fae6982abcRivera Monroy, Zuly Jennybcc909fcc85ff0efa659b7aaca42b3cbPineda Castañeda, Héctor Manuel94dc1b0c9bf5fa93da8185efbe9ce789Síntesis y Aplicación de Moléculas PeptídicasAplicaciones Analíticas de Compuestos Orgánicos (Aaco)0000-0002-9081-0546https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000073189https://www.scopus.com/authid/detail.uri?authorId=57208139842https://www.researchgate.net/profile/Hector-Pineda-Castanedahttps://scholar.google.com/citations?user=Wu60Xw0AAAAJ&hl=es&authuser=32024-02-07T13:53:46Z2024-02-07T13:53:46Z2023https://repositorio.unal.edu.co/handle/unal/85642Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/La resistencia a los antimicrobianos (RAM) es una de las diez principales amenazas para la salud pública reportadas por la Organización Mundial de la Salud (OMS). Una de las causas del creciente problema de la RAM es la falta de nuevas terapias y/o agentes de tratamiento; en consecuencia, muchas enfermedades infecciosas podrían volverse incontrolables. La necesidad de descubrir nuevos agentes antimicrobianos, que sean alternativos a los existentes, y que permitan mitigar este problema, se ha incrementado debido a la rápida y global expansión de la RAM. En este contexto, se han propuesto como alternativas para combatir la RAM tanto los péptidos antimicrobianos (PAMs) como los dendrímeros que presentan múltiples copias de compuestos antibacterianos en su estructura. Los dendrímeros han exhibido propiedades antifúngicas y antibacterianas y también se han utilizado en terapias antiinflamatorias, antineoplásicas y cardiovasculares y son útiles en sistemas de administración de fármacos y genes. En este trabajo se propuso obtener dendrímeros que presenten cuatro copias de secuencias de PAMs. Específicamente, (i) se exploró la síntesis, purificación y caracterización de dendrímeros de péptido-resorcinareno derivados de las secuencias LfcinB (20-25): RRWQWR y BF (32-35): RLLR, y (ii) la actividad antimicrobiana y citotóxica de estos dendrímeros. Se establecieron las rutas de síntesis que permitieron obtener: a) alquino-resorcinarenos y b) péptidos funcionalizados con el grupo azida; los cuales se usaron para generar c) dendrímeros de péptido-resorcinareno mediante química click de cicloadición de azida-alquino CuAAC. Finalmente, se evaluó la actividad antimicrobiana y citotóxica de los dendrímeros obtenidos frente a cepas de referencia y aislados clínicos. Estos resultados permitieron la identificación de moléculas antimicrobianas prometedoras que pueden conducir a avances en el desarrollo de nuevos agentes terapéuticos. (Texto tomado de la fuente)Antimicrobial resistance (AMR) is one of the top ten threats to public health reported by the World Health Organization (WHO). One of the causes of the growing AMR problem is the lack of new therapies and/or treatment agents; consequently, many infectious diseases could become uncontrollable. The need to discover new antimicrobial agents, which are alternatives to the existing ones, and which allow mitigating this problem, has increased due to the rapid and global expansion of AMR. In this context, both antimicrobial peptides (AMPs) and dendrimers that present multiple copies of antibacterial compounds in their structure have been proposed as alternatives to combat AMR. Dendrimers have exhibited antifungal and antibacterial properties and have also been used in anti-inflammatory, antineoplastic and cardiovascular therapies and are useful in drug and gene delivery systems. In this work, it was proposed to obtain dendrimers that present four copies of AMPs sequences. Specifically, (i) the synthesis, purification, and characterization of peptide-resorcinarene dendrimers derived from the sequences LfcinB (20-25): RRWQWR and BF (32-35): RLLR, and (ii) the antimicrobial and cytotoxic activity were explored. of these dendrimers. The synthesis routes that allowed obtaining: a) alkyne-resorcinarenes and b) peptides functionalized with the azide group were established, which were used to generate c) peptide-resorcinarene dendrimers by azide-alkyne cycloaddition (CuAAC) click chemistry. Finally, the antimicrobial and cytotoxic activity of the dendrimers obtained was evaluated against reference strains and clinical isolates. These results allowed the identification of promising antimicrobial molecules that may lead to breakthroughs in the development of new therapeutic agents.DoctoradoDoctor en Ciencias - Química269 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afinesProgramas de Optimización del Uso de los AntimicrobianosAntiinfecciososAntimicrobial StewardshipAnti-Infective AgentsDendrimerosPéptidoResorcinarenoPéptidos AntimicrobianosQuímica clickDendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacterianoPeptide-resorcinarene dendrimers: (i) obtaining by azide/alkyne cycloaddition reaction and (ii) evaluation of their antibacterial potentialTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06DataPaperhttp://purl.org/redcol/resource_type/TDJ. 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Guerra et al., “The tetrameric peptide LfcinB (20-25)4 derived from bovine lactoferricin induces apoptosis in the MCF-7 breast cancer cell line,” RSC Adv, vol. 9, no. 36, pp. 20497–20504, 2019, doi: 10.1039/c9ra04145aDiseño y obtención de nuevos agentes antibacterianos basados en dendrímeros péptido-resorcinareno: Una alternativa para combatir la resistencia bacterianaMincienciasInvestigadoresORIGINAL1016063614.2023.pdf1016063614.2023.pdfTesis de Doctorado en Ciencias - Químicaapplication/pdf49940176https://repositorio.unal.edu.co/bitstream/unal/85642/6/1016063614.2023.pdf43add30d4b4747a695ddb85fc20a415dMD56LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85642/5/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD55THUMBNAIL1016063614.2023.pdf.jpg1016063614.2023.pdf.jpgGenerated 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Dendrímeros péptido-resorcinareno: (i) obtención mediante reacción de cicloadición azida/alquino y (ii) evaluación de su potencial antibacteriano

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