Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica
ilustraciones, gráficas, tablas
- Autores:
-
Botero Buitrago, Jenny Alejandra
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84357
- Palabra clave:
- 570 - Biología::572 - Bioquímica
Antígenos Nucleares del Virus de Epstein-Barr
Ensayos de Selección de Medicamentos Antitumorales
Epstein-Barr Virus Nuclear Antigens
Drug Screening Assays, Antitumor
Citoquinas
Péptidos
Perfil de expresión
Inflamación
Cytokines
Peptides
Expression profile
Inflammation
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica |
| dc.title.translated.eng.fl_str_mv |
Effect of peptides derived from the Epstein-Barr virus gp85 protein on the expression of cytokines in peripheral blood mononuclear cells |
| title |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica |
| spellingShingle |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica 570 - Biología::572 - Bioquímica Antígenos Nucleares del Virus de Epstein-Barr Ensayos de Selección de Medicamentos Antitumorales Epstein-Barr Virus Nuclear Antigens Drug Screening Assays, Antitumor Citoquinas Péptidos Perfil de expresión Inflamación Cytokines Peptides Expression profile Inflammation |
| title_short |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica |
| title_full |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica |
| title_fullStr |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica |
| title_full_unstemmed |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica |
| title_sort |
Efecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periférica |
| dc.creator.fl_str_mv |
Botero Buitrago, Jenny Alejandra |
| dc.contributor.advisor.none.fl_str_mv |
Urquiza Martínez, Mauricio |
| dc.contributor.author.none.fl_str_mv |
Botero Buitrago, Jenny Alejandra |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Hormonas |
| dc.contributor.orcid.spa.fl_str_mv |
Botero Buitrago, Jenny [0000000199904648] |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::572 - Bioquímica |
| topic |
570 - Biología::572 - Bioquímica Antígenos Nucleares del Virus de Epstein-Barr Ensayos de Selección de Medicamentos Antitumorales Epstein-Barr Virus Nuclear Antigens Drug Screening Assays, Antitumor Citoquinas Péptidos Perfil de expresión Inflamación Cytokines Peptides Expression profile Inflammation |
| dc.subject.decs.spa.fl_str_mv |
Antígenos Nucleares del Virus de Epstein-Barr Ensayos de Selección de Medicamentos Antitumorales |
| dc.subject.decs.eng.fl_str_mv |
Epstein-Barr Virus Nuclear Antigens Drug Screening Assays, Antitumor |
| dc.subject.proposal.spa.fl_str_mv |
Citoquinas Péptidos Perfil de expresión Inflamación |
| dc.subject.proposal.eng.fl_str_mv |
Cytokines Peptides Expression profile Inflammation |
| description |
ilustraciones, gráficas, tablas |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-07-28T16:21:51Z |
| dc.date.available.none.fl_str_mv |
2023-07-28T16:21:51Z |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84357 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/84357 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Universidad Nacional de Colombia |
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Facultad de Ciencias |
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Atribución-NoComercial-SinDerivadas 4.0 InternacionalDerechos reservados al autor, 2023http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Urquiza Martínez, Mauricioe587bea6b995ab20cfac09ef61d49a2fBotero Buitrago, Jenny Alejandra99039ff504a8958962ab3a2b7bb84fa1Grupo de Investigación en HormonasBotero Buitrago, Jenny [0000000199904648]2023-07-28T16:21:51Z2023-07-28T16:21:51Z2023https://repositorio.unal.edu.co/handle/unal/84357Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficas, tablasLas citoquinas son proteínas involucradas principalmente en la comunicación intercelular durante la respuesta inmune. Algunas citoquinas pueden inhibir el desarrollo y progresión de tumores, y estos efectos parecen estar relacionados con la modulación de la respuesta antitumoral. Estudios previos han identificado moléculas capaces de regular su expresión, entre ellas, un péptido derivado del sitio de unión de la glicoproteína gp85 del virus de Epstein-Barr a leucocitos humanos, denominado 11438. En este trabajo, se evaluó el efecto de este péptido y su análogo (33210) sobre la producción de citoquinas pro y antiinflamatorias, a nivel de ARNm y de proteína, en células mononucleares de sangre periférica (PBMCs) sanas, así como la inducción de cambios fenotípicos en esta población celular. Se determinó que los péptidos inducen un aumento en la expresión génica de citoquinas como IL-12B e IL-4, y que el péptido 33210 modificó el perfil de expresión de citoquinas a nivel de proteína al aumentar la producción de citoquinas inflamatorias como TNF-α, IL-8 e IL-6. Con relación a los marcadores de superficie de linfocitos y monocitos específicamente, se estableció una tendencia que indica que los péptidos modifican su expresión, indicando una regulación continua de la respuesta inmune. Estos resultados sugieren que los péptidos evaluados pueden actuar como moléculas promisorias para ayudar a la erradicación de células tumorales, en tanto inducen una activación de la respuesta inmune mediada por la expresión de citoquinas pro y antiinflamatorias. (Texto tomado de la fuente)Cytokines are proteins mainly involved in intercellular communication during the immune response. Some cytokines can inhibit the development and progression of tumors, and these effects seem to be related to the modulation of the antitumor response. Previous studies have identified molecules capable of regulating its expression, among them, a peptide derived from the binding site of the glycoprotein gp85 of the Epstein-Barr virus to human leukocytes, called 11438. In this work, the effect of this peptide was evaluated and its analogue (33210) on the production of pro- and anti-inflammatory cytokines, at the mRNA and protein level, in healthy peripheral blood mononuclear cells (PBMCs), as well as the induction of phenotypic changes in this cell population. It was determined that the peptides induce an increase in the gene expression of cytokines such as IL-12B and IL-4, and that peptide 33210 modified the cytokine expression profile at the protein level by increasing the production of inflammatory cytokines such as TNF-α, IL-8 and IL-6. Regarding the surface markers of lymphocytes and monocytes specifically, a trend was established indicating that the peptides modify their expression, indicating a continuous regulation of the immune response. These results suggest that the peptides evaluated may act as promising molecules to help eradicate tumor cells, while inducing an activation of the immune response mediated by the expression of pro- and anti-inflammatory cytokines.MaestríaMagíster en Ciencias - BioquímicaBioactividadxiv, 78 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - BioquímicaAntígenos Nucleares del Virus de Epstein-BarrEnsayos de Selección de Medicamentos AntitumoralesEpstein-Barr Virus Nuclear AntigensDrug Screening Assays, AntitumorCitoquinasPéptidosPerfil de expresiónInflamaciónCytokinesPeptidesExpression profileInflammationEfecto de péptidos derivados de la proteína gp85 del virus de Epstein-Barr sobre la expresión de citoquinas en células mononucleares de sangre periféricaEffect of peptides derived from the Epstein-Barr virus gp85 protein on the expression of cytokines in peripheral blood mononuclear cellsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMShaw DM, Merien F, Braakhuis A, Dulson D. 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Dendritic cell differentiation induced by a self-peptide derived from Apolipoprotein E. jo. 2008;181(10):6859–71.EstudiantesInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84357/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1026297033.2023.pdf1026297033.2023.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf2976733https://repositorio.unal.edu.co/bitstream/unal/84357/2/1026297033.2023.pdf2db99db9c6e3197e36083bddff728e42MD52THUMBNAIL1026297033.2023.pdf.jpg1026297033.2023.pdf.jpgGenerated Thumbnailimage/jpeg6003https://repositorio.unal.edu.co/bitstream/unal/84357/3/1026297033.2023.pdf.jpg9821ea68f09e2800d8155d804e86075eMD53unal/84357oai:repositorio.unal.edu.co:unal/843572023-08-15 23:03:47.816Repositorio Institucional Universidad Nacional de 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