Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8

In this work the relevance of a systematic replacement of peptide-bonds on the Mastoparan MP-8 antimicrobial peptide to afford 27 new pseudopeptide analogues is reported. Results allowed to determine that pseudopeptides ψ-38617 (INLKALAALAKd-[CH2NH]-RLL), ψ-38629(INLKAd-[CH2NH]-LAALAKRLL) and ψ-3863...

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Autores:
Carreño, Luisa Fernanda
Lozano, José Manuel
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/49320
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/49320
http://bdigital.unal.edu.co/42777/
http://bdigital.unal.edu.co/42777/2/
Palabra clave:
péptido antimicrobiano
Mastoparán MP-8
actividad hemolítica
estabilidad
antimicrobial peptide
Mastoparan MP-8
hemolytic activity
stability
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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oai_identifier_str oai:repositorio.unal.edu.co:unal/49320
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Carreño, Luisa Fernandacd61ba98-1a85-4468-9cac-3c00599126b0300Lozano, José Manuelf7ee3a81-06b2-44b1-9874-954e1e8f9dc83002019-06-29T08:35:16Z2019-06-29T08:35:16Z2013https://repositorio.unal.edu.co/handle/unal/49320http://bdigital.unal.edu.co/42777/http://bdigital.unal.edu.co/42777/2/In this work the relevance of a systematic replacement of peptide-bonds on the Mastoparan MP-8 antimicrobial peptide to afford 27 new pseudopeptide analogues is reported. Results allowed to determine that pseudopeptides ψ-38617 (INLKALAALAKd-[CH2NH]-RLL), ψ-38629(INLKAd-[CH2NH]-LAALAKRLL) and ψ-38630 (INLK-[CH2NH]-ALAALAKRLL), showed an enhanced anti gam-negative bacterial properties, regarding the native Mastoparan MP-8 peptide, but maintaining a comparable activity against gram-positive bacteria. In addition, specific performed modifications on MP-8 sequence led three new molecules that completely abolished the hemolytic activity of the parent MP-8 peptide. All obtained molecules possess a high stability profile when tested against a severe proteolytic attack.En este trabajo se reporta el efecto, la modificación puntual y sistemática a nivel del enlace peptídico realizada al péptido Mastoparán MP-8, obteniéndose 27 pseudopéptidos en total. Los resultados permitieron determinar que los pseudopéptidos ψ-38617(INLKALAALAKd-[CH2NH]-RLL), ψ-38629(INLKAd-[CH2NH]-LAALAKRLL) y ψ-38630 (INLK-[CH2NH]-ALAALAKRLL) presentan mejor actividad que el péptido Mastoparán MP-8 frente a las bacterias Gram negativas y conservaron la actividad antibacteriana frente a las bacterias Gram positivas; además, la modificación realizada produjo tres moléculas que abolieron totalmente la actividad hemolítica propia del péptido nativo. Todas las modificaciones realizadas a esta secuencia provocaron un aumento en la estabilidad de las moléculas frente al ataque enzimático.application/pdfspaRevista Colombiana de Ciencias Químico Farmacéuticashttp://revistas.unal.edu.co/index.php/rccquifa/article/view/45103Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Ciencias Químico FarmacéuticasRevista Colombiana de Ciencias Químico FarmacéuticasRevista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 56-79 0034-7418 1909-6356Carreño, Luisa Fernanda and Lozano, José Manuel (2013) Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8. Revista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 56-79 0034-7418 1909-6356 .Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTpéptido antimicrobianoMastoparán MP-8actividad hemolíticaestabilidadantimicrobial peptideMastoparan MP-8hemolytic activitystabilityORIGINAL45103-216312-1-SM.pdfapplication/pdf1167680https://repositorio.unal.edu.co/bitstream/unal/49320/1/45103-216312-1-SM.pdfc0d7172bf1eadf109e69fb062e705f6fMD51THUMBNAIL45103-216312-1-SM.pdf.jpg45103-216312-1-SM.pdf.jpgGenerated Thumbnailimage/jpeg5528https://repositorio.unal.edu.co/bitstream/unal/49320/2/45103-216312-1-SM.pdf.jpg694e2a747956b55dcaa4a677034bdcfeMD52unal/49320oai:repositorio.unal.edu.co:unal/493202022-12-14 23:03:55.032Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
title Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
spellingShingle Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
péptido antimicrobiano
Mastoparán MP-8
actividad hemolítica
estabilidad
antimicrobial peptide
Mastoparan MP-8
hemolytic activity
stability
title_short Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
title_full Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
title_fullStr Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
title_full_unstemmed Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
title_sort Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8
dc.creator.fl_str_mv Carreño, Luisa Fernanda
Lozano, José Manuel
dc.contributor.author.spa.fl_str_mv Carreño, Luisa Fernanda
Lozano, José Manuel
dc.subject.proposal.spa.fl_str_mv péptido antimicrobiano
Mastoparán MP-8
actividad hemolítica
estabilidad
antimicrobial peptide
Mastoparan MP-8
hemolytic activity
stability
topic péptido antimicrobiano
Mastoparán MP-8
actividad hemolítica
estabilidad
antimicrobial peptide
Mastoparan MP-8
hemolytic activity
stability
description In this work the relevance of a systematic replacement of peptide-bonds on the Mastoparan MP-8 antimicrobial peptide to afford 27 new pseudopeptide analogues is reported. Results allowed to determine that pseudopeptides ψ-38617 (INLKALAALAKd-[CH2NH]-RLL), ψ-38629(INLKAd-[CH2NH]-LAALAKRLL) and ψ-38630 (INLK-[CH2NH]-ALAALAKRLL), showed an enhanced anti gam-negative bacterial properties, regarding the native Mastoparan MP-8 peptide, but maintaining a comparable activity against gram-positive bacteria. In addition, specific performed modifications on MP-8 sequence led three new molecules that completely abolished the hemolytic activity of the parent MP-8 peptide. All obtained molecules possess a high stability profile when tested against a severe proteolytic attack.
publishDate 2013
dc.date.issued.spa.fl_str_mv 2013
dc.date.accessioned.spa.fl_str_mv 2019-06-29T08:35:16Z
dc.date.available.spa.fl_str_mv 2019-06-29T08:35:16Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/49320
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/42777/
http://bdigital.unal.edu.co/42777/2/
url https://repositorio.unal.edu.co/handle/unal/49320
http://bdigital.unal.edu.co/42777/
http://bdigital.unal.edu.co/42777/2/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/rccquifa/article/view/45103
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Ciencias Químico Farmacéuticas
Revista Colombiana de Ciencias Químico Farmacéuticas
dc.relation.ispartofseries.none.fl_str_mv Revista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 56-79 0034-7418 1909-6356
dc.relation.references.spa.fl_str_mv Carreño, Luisa Fernanda and Lozano, José Manuel (2013) Structural exploration of antibacterial activity and hemolytic profiles of non-natural analogs of the antimicrobial peptide mastoparan mp-8. Revista Colombiana de Ciencias Químico Farmacéuticas; Vol. 42, núm. 1 (2013); 56-79 0034-7418 1909-6356 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Revista Colombiana de Ciencias Químico Farmacéuticas
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/49320/1/45103-216312-1-SM.pdf
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