Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia

En los últimos años en Colombia, los cultivos de gulupa (Passiflora edulis f. edulis Sims) y uchuva (Physalis peruviana L.) han tomado gran relevancia en el sector agrícola de la región andina. Esto se debe a que sus frutos presentan características organolépticas y nutricionales muy apetecidas en e...

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Autores:: Bacca David, Michelle

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
dc.title.translated.eng.fl_str_mv	Molecular characterization of viruses in crops of cape gooseberry (Physalis peruviana) and purple passion fruit (Passiflora edulis f. edulis) in southwestern Antioquia
title	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
spellingShingle	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Uchuva (Physalis peruviana) - Enfermedades y plagas Virus fitopatógenos Cultivo in vitro RT-qPCR Secuenciación masiva Passiflora edulis f. edulis Physalis peruviana virus fitopatógenos Cultivo in vitro Uchuva (Physalis peruviana) Gulupa (Passiflora edulis f. edulis) High-throughput sequencing plant viruses In vitro plant tissue
title_short	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_full	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_fullStr	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_full_unstemmed	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_sort	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
dc.creator.fl_str_mv	Bacca David, Michelle
dc.contributor.advisor.none.fl_str_mv	Marín Montoya, Mauricio Alejandro Gutiérrez Sánchez, Pablo Andrés
dc.contributor.author.none.fl_str_mv	Bacca David, Michelle
dc.contributor.researcher.none.fl_str_mv	Higuita Valencia, Monica Gallo Garcia, Yuliana Restrepo Escobar, Andrea Posada Rua,Jaime Giraldo Ramirez, Susana Martinez Mira, Anny Bacca David, Michelle Restrepo, Andrea Marín, Mauricio Gutiérrez, Pablo
dc.contributor.researchgroup.spa.fl_str_mv	Biotecnología vegetal
dc.contributor.orcid.spa.fl_str_mv	Bacca, Michelle [0000-0002-8277-9593]
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
topic	630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Uchuva (Physalis peruviana) - Enfermedades y plagas Virus fitopatógenos Cultivo in vitro RT-qPCR Secuenciación masiva Passiflora edulis f. edulis Physalis peruviana virus fitopatógenos Cultivo in vitro Uchuva (Physalis peruviana) Gulupa (Passiflora edulis f. edulis) High-throughput sequencing plant viruses In vitro plant tissue
dc.subject.agrovoc.none.fl_str_mv	Uchuva (Physalis peruviana) - Enfermedades y plagas
dc.subject.lemb.none.fl_str_mv	Virus fitopatógenos Cultivo in vitro
dc.subject.proposal.spa.fl_str_mv	RT-qPCR Secuenciación masiva Passiflora edulis f. edulis Physalis peruviana virus fitopatógenos Cultivo in vitro Uchuva (Physalis peruviana) Gulupa (Passiflora edulis f. edulis)
dc.subject.proposal.eng.fl_str_mv	High-throughput sequencing plant viruses In vitro plant tissue
description	En los últimos años en Colombia, los cultivos de gulupa (Passiflora edulis f. edulis Sims) y uchuva (Physalis peruviana L.) han tomado gran relevancia en el sector agrícola de la región andina. Esto se debe a que sus frutos presentan características organolépticas y nutricionales muy apetecidas en el mercado internacional y nacional. El aumento del área sembrada de estos frutales ha estado acompañado del incremento de diversos problemas fitosanitarios, entre los que se destacan aquellos de origen viral. Sin embargo, el conocimiento de los agentes causales, vectores y efectos sobre los rendimientos es aún incipiente para estas enfermedades. Esta información es fundamental para proponer programas de manejo integrado que resulten en aumentos en la productividad y longevidad de los cultivos, así como en la calidad de los frutos. Con el fin de contribuir al aumento del conocimiento sobre el viroma de estos cultivos, en este proyecto de investigación se planteó la utilización de metodologías moleculares de última generación para determinar los virus más prevalentes en cultivos y material de siembra de gulupa y uchuva del Suroeste de Antioquia; adicionalmente, se realizó una evaluación inicial de posibles vectores y reservorios biológicos (arvenses) para dichos virus. Los resultados obtenidos indicaron que PMTV fue el virus más prevalente en los cultivos de uchuva, ya que se detectó en el 100% de muestras sintomáticas, 80% de muestras asintomáticas y en el 60% de las muestras de semillas. Los análisis HTS revelaron la presencia de una nueva especie de virus estrechamente relacionada con el género Trichovirus, denominada tentativamente como Physalis chlorosis virus (PhyCV). También se obtuvo la secuencia del genoma de nuevos aislamientos de TaLMV, PhyVNV y PVY que afectan a P. peruviana. Por otra parte, mediante PCR y confirmación morfológica, fue posible identificar en el cultivo de uchuva ocho especies de plantas arvenses: Commelina diffusa, Ageratum conyzoides, Erigeron sumatrensis, Galinsoga quadriradiata, Bidens pilosa, Sonchus oleraceus, Persicaria nepalensis y Plantago australis; así como dos especies de insectos posiblemente vectores de virus: Trialeurodes vaporariorum y Frankliniella occidentalis. Utilizando la prueba de RT-qPCR con cebadores específicos, se detectó la ocurrencia de los virus PVS, PVX, TaLMV y PMTV en algunas arvenses asociadas al cultivo de uchuva. En el culltivo de gulupa, se detectaron los virus CMV, PFYMV, PpLDV y GBVA tanto en muestras de campo como en material de siembra; los virus CABMV y SMV no fueron encontrados en ninguna de las muestras analizadas. Para el caso de arvenses, en los cultivos de gulupa del Suroeste se identificaron nueve especies: Persicaria nepalensis, Commelina diffusa, Cardamine flexuosa, Galinsoga quadriradiata, Bidens pilosa, Ageratum conyzoides, Erigeron sumatrensis, Sonchus oleraceus e Ipomoea purpurea, así como seis posibles insectos vectores de virus: Lachnopus sp., Aphis fabae, Tetranychus sp., Pseudococcus sp., Brachycaudus helichrysi y Neohydatothrips burungae. También se detectó al PFYMV y GBVA en algunas de las arvenses y el CMV en el insecto A. fabae. Finalmente, se realizó una prueba inicial de la técnica aislamiento/cultivo de meristemos in vitro en uchuva para la eliminación viral, obteniéndo vitroplántulas libres de los virus PVY, PVS, PVX y CGIV-1. En gulupa se empleó quimioterapia ex vitro en plántulas bajo diferentes concentraciones de ribavirina (225, 250 y 275 ppm) en un tiempo de inmersión radicular de 2 h y 30 min; sin embargo, con este tratamiento no se logró obtener plántulas libres de PFYMV, pero sí con menor titulo viral, determinado en términos de valores de Ct en pruebas de RT-qPCR. Estos resultados señalan la importancia de implementar conjuntamente métodos de secuenciación masiva y técnicas moleculares como herramientas eficientes para el diagnóstico de infecciones virales que apoyen los programas de manejo integrado de enfermedades virales, vigilancia curentenaria y obtención de material certificado en estos frutales andinos. (Texto tomado de la fuente)
publishDate	2022
dc.date.issued.none.fl_str_mv	2022
dc.date.accessioned.none.fl_str_mv	2023-02-10T18:33:49Z
dc.date.available.none.fl_str_mv	2023-02-10T18:33:49Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/83420
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/83420 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.indexed.spa.fl_str_mv	LaReferencia
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Gigascience 8(9): giz100. doi: 10.1093/gigascience/giz100 Cardona D, Higuita M, Posada J, Gallo Y, Marín M, Gutiérrez P (2022a). Viruses infecting purple passion fruit (Passiflora edulis f. edulis) in Southwestern Antioquia (Colombia). Archives of Phytopathology and Plant Protection. Archives of Phytopathology and Plant Protection, 55(12): 1394–1409. doi: 10.1080/03235408.2022.2098588 Cardona, D., Restrepo, A., Higuita, M., Gallo, Y., Marin, M., y Gutiérrez, P. (2022b). Natural infection of purple passion fruit (Passiflora edulis f. edulis) by a novel member of the family Tymoviridae in Colombia. Acta virologica, 66(3):254–262. doi:10.4149/av_2022_310 Cardona D, Gallo Y, Higuita M, Hoyos R, Gutiérrez P, Marín M (2022c). Detección molecular de virus en cultivos, plántulas y semillas de gulupa (Passiflora edulis f. edulis) en el oriente de Antioquia. Bioagro: 34(2). Chabannes M, Gabriel M, Aksa A, Galzi S, Dufayard JF, Iskra-Caruana ML, Muller E (2020). 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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Marín Montoya, Mauricio Alejandroea05ef2dc8f58ea502fc56bf288d0ab9600Gutiérrez Sánchez, Pablo Andrés03fa1cb1dfa744c01ad629ca741ddd94600Bacca David, Michelle0780ffaa143d32044f6d699a10039767Higuita Valencia, MonicaGallo Garcia, YulianaRestrepo Escobar, AndreaPosada Rua,JaimeGiraldo Ramirez, SusanaMartinez Mira, AnnyBacca David, MichelleRestrepo, AndreaMarín, MauricioGutiérrez, PabloBiotecnología vegetalBacca, Michelle [0000-0002-8277-9593]2023-02-10T18:33:49Z2023-02-10T18:33:49Z2022https://repositorio.unal.edu.co/handle/unal/83420Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/En los últimos años en Colombia, los cultivos de gulupa (Passiflora edulis f. edulis Sims) y uchuva (Physalis peruviana L.) han tomado gran relevancia en el sector agrícola de la región andina. Esto se debe a que sus frutos presentan características organolépticas y nutricionales muy apetecidas en el mercado internacional y nacional. El aumento del área sembrada de estos frutales ha estado acompañado del incremento de diversos problemas fitosanitarios, entre los que se destacan aquellos de origen viral. Sin embargo, el conocimiento de los agentes causales, vectores y efectos sobre los rendimientos es aún incipiente para estas enfermedades. Esta información es fundamental para proponer programas de manejo integrado que resulten en aumentos en la productividad y longevidad de los cultivos, así como en la calidad de los frutos. Con el fin de contribuir al aumento del conocimiento sobre el viroma de estos cultivos, en este proyecto de investigación se planteó la utilización de metodologías moleculares de última generación para determinar los virus más prevalentes en cultivos y material de siembra de gulupa y uchuva del Suroeste de Antioquia; adicionalmente, se realizó una evaluación inicial de posibles vectores y reservorios biológicos (arvenses) para dichos virus. Los resultados obtenidos indicaron que PMTV fue el virus más prevalente en los cultivos de uchuva, ya que se detectó en el 100% de muestras sintomáticas, 80% de muestras asintomáticas y en el 60% de las muestras de semillas. Los análisis HTS revelaron la presencia de una nueva especie de virus estrechamente relacionada con el género Trichovirus, denominada tentativamente como Physalis chlorosis virus (PhyCV). También se obtuvo la secuencia del genoma de nuevos aislamientos de TaLMV, PhyVNV y PVY que afectan a P. peruviana. Por otra parte, mediante PCR y confirmación morfológica, fue posible identificar en el cultivo de uchuva ocho especies de plantas arvenses: Commelina diffusa, Ageratum conyzoides, Erigeron sumatrensis, Galinsoga quadriradiata, Bidens pilosa, Sonchus oleraceus, Persicaria nepalensis y Plantago australis; así como dos especies de insectos posiblemente vectores de virus: Trialeurodes vaporariorum y Frankliniella occidentalis. Utilizando la prueba de RT-qPCR con cebadores específicos, se detectó la ocurrencia de los virus PVS, PVX, TaLMV y PMTV en algunas arvenses asociadas al cultivo de uchuva. En el culltivo de gulupa, se detectaron los virus CMV, PFYMV, PpLDV y GBVA tanto en muestras de campo como en material de siembra; los virus CABMV y SMV no fueron encontrados en ninguna de las muestras analizadas. Para el caso de arvenses, en los cultivos de gulupa del Suroeste se identificaron nueve especies: Persicaria nepalensis, Commelina diffusa, Cardamine flexuosa, Galinsoga quadriradiata, Bidens pilosa, Ageratum conyzoides, Erigeron sumatrensis, Sonchus oleraceus e Ipomoea purpurea, así como seis posibles insectos vectores de virus: Lachnopus sp., Aphis fabae, Tetranychus sp., Pseudococcus sp., Brachycaudus helichrysi y Neohydatothrips burungae. También se detectó al PFYMV y GBVA en algunas de las arvenses y el CMV en el insecto A. fabae. Finalmente, se realizó una prueba inicial de la técnica aislamiento/cultivo de meristemos in vitro en uchuva para la eliminación viral, obteniéndo vitroplántulas libres de los virus PVY, PVS, PVX y CGIV-1. En gulupa se empleó quimioterapia ex vitro en plántulas bajo diferentes concentraciones de ribavirina (225, 250 y 275 ppm) en un tiempo de inmersión radicular de 2 h y 30 min; sin embargo, con este tratamiento no se logró obtener plántulas libres de PFYMV, pero sí con menor titulo viral, determinado en términos de valores de Ct en pruebas de RT-qPCR. Estos resultados señalan la importancia de implementar conjuntamente métodos de secuenciación masiva y técnicas moleculares como herramientas eficientes para el diagnóstico de infecciones virales que apoyen los programas de manejo integrado de enfermedades virales, vigilancia curentenaria y obtención de material certificado en estos frutales andinos. (Texto tomado de la fuente)In recent years, purple passion fruit (Passiflora edulis f. edulis Sims) and cape gooseberry (Physalis peruviana L.) have become some of the most important crops in the Andean region of Colombia. Due to their unique organoleptic characteristics, both fruits have an increasingly high demand in local and international markets. However, the rapid expansion of these crops has resulted in a significant increase of several phytopathological problems, particularly viral diseases. Unfortunately, little is known about the viruses, their associated vectors and/or their effect on yields in both crops. This type of information is key for the implementation of integrated disease management programs aimed at improving the productivity and longevity of crops, as well as the quality of fruits. To increase our current knowledge on the virology of these crops, this work intended to determine which viruses are the most prevalent in commercial fields and planting material of purple passion fruit and cape gooseberry in southwestern Antioquia using state of the art molecular techniques. In addition, a preliminary investigation on potential vectors and plant weed reservoirs to these viruses was also performed. In cape gooseberry, results indicate that PMTV is the most prevalent virus as it was detected in 100% of symptomatic plants, 80% of asymptomatic plants, and 60% of seed samples. High-throughput sequencing revealed a new viral species related to the genus Trichovirus tentatively named Physalis chlorosis virus (PhyCV). Genome information on TaLMV, PhyVNV and PVY circulating in the region was also obtained. With respect to potential alternate hosts and vectors, a combination of standard PCR with morphological analyses revealed eight weeds associated with P. peruviana: Commelina diffusa, Ageratum conyzoides, Erigeron sumatrensis, Galinsoga quadriradiata, Bidens pilosa, Sonchus oleraceus, Persicaria nepalensis and Plantago australis; and two potential insect vectors: Trialeurodes vaporariorum and Frankliniella occidentalis. On the other hand, RT-qPCR analysis detected PVS, PVX, TaLMV and PMTV is some of the associated weeds. With respect to purple passion fruit, the viruses CMV, PFYMV, PpLDV and GBVA were detected in field samples and planting material; however, no evidence from the presence of CABMV nor SMV was found in any of the tested samples. Nine weed plants associated to purple passion fruit crops were identified in southwestern Antioquia: Persicaria nepalensis, Commelina diffusa, Cardamine flexuosa, Galinsoga quadriradiata, Bidens pilosa, Ageratum conyzoides, Erigeron sumatrensis, Sonchus oleraceus and Ipomoea purpurea. Six potential insect vectors were also identified in this crop: Lachnopus sp., Aphis fabae, Tetranychus sp., Pseudococcus sp., Brachycaudus helichrysi and Neohydatothrips burungae. RT-qPCR detected PFYMV and GBVA in some weeds, and CMV in the insect A. fabae. Finally, a preliminary test of virus clean-up methods using in vitro meristem culture in cape gooseberry resulted in the generation of vitroplants negative for PVY, PVS, PVX and CGIV-1. In purple passion fruit, evaluation of ribavirin chemotherapy (225, 250 y 275 ppm) of ex vitro plantlets at one immersion time (2 h and 30 min) failed to eliminate the virus PFYMV; however, results suggest that this method does reduce the viral load for this virus as evidenced by RT-qPCR. Together, these results highlight the importance of using HTS in combination with molecular techniques for reliable testing of viruses and to support disease management programs, quarantine vigilance and the generation of certified planting material of these important fruit crops.Universidad Nacional de Colombia sede Medellín, Universidad CES y Caribbean Exotics.MaestríaMagíster en Ciencias - BiotecnologíaVirología vegetalÁrea curricular Biotecnologíaxii, 222 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesUchuva (Physalis peruviana) - Enfermedades y plagasVirus fitopatógenosCultivo in vitroRT-qPCRSecuenciación masivaPassiflora edulis f. edulisPhysalis peruvianavirus fitopatógenosCultivo in vitroUchuva (Physalis peruviana)Gulupa (Passiflora edulis f. edulis)High-throughput sequencingplant virusesIn vitro plant tissueCaracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de AntioquiaMolecular characterization of viruses in crops of cape gooseberry (Physalis peruviana) and purple passion fruit (Passiflora edulis f. edulis) in southwestern AntioquiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMLaReferenciaInternational Committee on Taxonomy of Viruses [ICTV]. 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Journal of Plant Pathology 97(2): 391-403. doi: 10.4454/JPP.V97I2.00(Proyecto 1101-805-62786, Convenio No. 4600007658-779) " Detección, caracterización y limpieza de virus y otros fitopatógenos en material de siembra y cultivos de gulupa (Passiflora edulis f. edulis) y uchuva (Physalis peruviana) en Antioquia"Fondo de Ciencia, Tecnología e Innovación del Sistema General de Regalías del Departamento de AntioquiaAdministradoresBibliotecariosConsejerosEstudiantesGrupos comunitariosInvestigadoresMaestrosMedios de comunicaciónPadres y familiasPersonal de apoyo escolarProveedores de ayuda financiera para estudiantesPúblico generalReceptores de fondos federales y solicitantesResponsables políticosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83420/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53CC-LICENSEBacca David Michelle.licencia.cap.1.pdfBacca David 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Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia

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