Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia

En los últimos años en Colombia, los cultivos de gulupa (Passiflora edulis f. edulis Sims) y uchuva (Physalis peruviana L.) han tomado gran relevancia en el sector agrícola de la región andina. Esto se debe a que sus frutos presentan características organolépticas y nutricionales muy apetecidas en e...

Full description

Autores:: Bacca David, Michelle

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

id	UNACIONAL2_23473521f348466a233fe81581fca876
oai_identifier_str	oai:repositorio.unal.edu.co:unal/83420
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
dc.title.translated.eng.fl_str_mv	Molecular characterization of viruses in crops of cape gooseberry (Physalis peruviana) and purple passion fruit (Passiflora edulis f. edulis) in southwestern Antioquia
title	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
spellingShingle	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Uchuva (Physalis peruviana) - Enfermedades y plagas Virus fitopatógenos Cultivo in vitro RT-qPCR Secuenciación masiva Passiflora edulis f. edulis Physalis peruviana virus fitopatógenos Cultivo in vitro Uchuva (Physalis peruviana) Gulupa (Passiflora edulis f. edulis) High-throughput sequencing plant viruses In vitro plant tissue
title_short	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_full	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_fullStr	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_full_unstemmed	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
title_sort	Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia
dc.creator.fl_str_mv	Bacca David, Michelle
dc.contributor.advisor.none.fl_str_mv	Marín Montoya, Mauricio Alejandro Gutiérrez Sánchez, Pablo Andrés
dc.contributor.author.none.fl_str_mv	Bacca David, Michelle
dc.contributor.researcher.none.fl_str_mv	Higuita Valencia, Monica Gallo Garcia, Yuliana Restrepo Escobar, Andrea Posada Rua,Jaime Giraldo Ramirez, Susana Martinez Mira, Anny Bacca David, Michelle Restrepo, Andrea Marín, Mauricio Gutiérrez, Pablo
dc.contributor.researchgroup.spa.fl_str_mv	Biotecnología vegetal
dc.contributor.orcid.spa.fl_str_mv	Bacca, Michelle [0000-0002-8277-9593]
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
topic	630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Uchuva (Physalis peruviana) - Enfermedades y plagas Virus fitopatógenos Cultivo in vitro RT-qPCR Secuenciación masiva Passiflora edulis f. edulis Physalis peruviana virus fitopatógenos Cultivo in vitro Uchuva (Physalis peruviana) Gulupa (Passiflora edulis f. edulis) High-throughput sequencing plant viruses In vitro plant tissue
dc.subject.agrovoc.none.fl_str_mv	Uchuva (Physalis peruviana) - Enfermedades y plagas
dc.subject.lemb.none.fl_str_mv	Virus fitopatógenos Cultivo in vitro
dc.subject.proposal.spa.fl_str_mv	RT-qPCR Secuenciación masiva Passiflora edulis f. edulis Physalis peruviana virus fitopatógenos Cultivo in vitro Uchuva (Physalis peruviana) Gulupa (Passiflora edulis f. edulis)
dc.subject.proposal.eng.fl_str_mv	High-throughput sequencing plant viruses In vitro plant tissue
description	En los últimos años en Colombia, los cultivos de gulupa (Passiflora edulis f. edulis Sims) y uchuva (Physalis peruviana L.) han tomado gran relevancia en el sector agrícola de la región andina. Esto se debe a que sus frutos presentan características organolépticas y nutricionales muy apetecidas en el mercado internacional y nacional. El aumento del área sembrada de estos frutales ha estado acompañado del incremento de diversos problemas fitosanitarios, entre los que se destacan aquellos de origen viral. Sin embargo, el conocimiento de los agentes causales, vectores y efectos sobre los rendimientos es aún incipiente para estas enfermedades. Esta información es fundamental para proponer programas de manejo integrado que resulten en aumentos en la productividad y longevidad de los cultivos, así como en la calidad de los frutos. Con el fin de contribuir al aumento del conocimiento sobre el viroma de estos cultivos, en este proyecto de investigación se planteó la utilización de metodologías moleculares de última generación para determinar los virus más prevalentes en cultivos y material de siembra de gulupa y uchuva del Suroeste de Antioquia; adicionalmente, se realizó una evaluación inicial de posibles vectores y reservorios biológicos (arvenses) para dichos virus. Los resultados obtenidos indicaron que PMTV fue el virus más prevalente en los cultivos de uchuva, ya que se detectó en el 100% de muestras sintomáticas, 80% de muestras asintomáticas y en el 60% de las muestras de semillas. Los análisis HTS revelaron la presencia de una nueva especie de virus estrechamente relacionada con el género Trichovirus, denominada tentativamente como Physalis chlorosis virus (PhyCV). También se obtuvo la secuencia del genoma de nuevos aislamientos de TaLMV, PhyVNV y PVY que afectan a P. peruviana. Por otra parte, mediante PCR y confirmación morfológica, fue posible identificar en el cultivo de uchuva ocho especies de plantas arvenses: Commelina diffusa, Ageratum conyzoides, Erigeron sumatrensis, Galinsoga quadriradiata, Bidens pilosa, Sonchus oleraceus, Persicaria nepalensis y Plantago australis; así como dos especies de insectos posiblemente vectores de virus: Trialeurodes vaporariorum y Frankliniella occidentalis. Utilizando la prueba de RT-qPCR con cebadores específicos, se detectó la ocurrencia de los virus PVS, PVX, TaLMV y PMTV en algunas arvenses asociadas al cultivo de uchuva. En el culltivo de gulupa, se detectaron los virus CMV, PFYMV, PpLDV y GBVA tanto en muestras de campo como en material de siembra; los virus CABMV y SMV no fueron encontrados en ninguna de las muestras analizadas. Para el caso de arvenses, en los cultivos de gulupa del Suroeste se identificaron nueve especies: Persicaria nepalensis, Commelina diffusa, Cardamine flexuosa, Galinsoga quadriradiata, Bidens pilosa, Ageratum conyzoides, Erigeron sumatrensis, Sonchus oleraceus e Ipomoea purpurea, así como seis posibles insectos vectores de virus: Lachnopus sp., Aphis fabae, Tetranychus sp., Pseudococcus sp., Brachycaudus helichrysi y Neohydatothrips burungae. También se detectó al PFYMV y GBVA en algunas de las arvenses y el CMV en el insecto A. fabae. Finalmente, se realizó una prueba inicial de la técnica aislamiento/cultivo de meristemos in vitro en uchuva para la eliminación viral, obteniéndo vitroplántulas libres de los virus PVY, PVS, PVX y CGIV-1. En gulupa se empleó quimioterapia ex vitro en plántulas bajo diferentes concentraciones de ribavirina (225, 250 y 275 ppm) en un tiempo de inmersión radicular de 2 h y 30 min; sin embargo, con este tratamiento no se logró obtener plántulas libres de PFYMV, pero sí con menor titulo viral, determinado en términos de valores de Ct en pruebas de RT-qPCR. Estos resultados señalan la importancia de implementar conjuntamente métodos de secuenciación masiva y técnicas moleculares como herramientas eficientes para el diagnóstico de infecciones virales que apoyen los programas de manejo integrado de enfermedades virales, vigilancia curentenaria y obtención de material certificado en estos frutales andinos. (Texto tomado de la fuente)
publishDate	2022
dc.date.issued.none.fl_str_mv	2022
dc.date.accessioned.none.fl_str_mv	2023-02-10T18:33:49Z
dc.date.available.none.fl_str_mv	2023-02-10T18:33:49Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/83420
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/83420 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.indexed.spa.fl_str_mv	LaReferencia
dc.relation.references.spa.fl_str_mv	International Committee on Taxonomy of Viruses [ICTV]. (2022). Bromoviridae- Positive Sense RNA Viruses. https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/bromoviridae International Committee on Taxonomy of Viruses [ICTV]. (2022). Potexvirus - Alphaflexiviridae - Positive-sense RNA Viruses.https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/alphaflexiviridae/1330/genus-potexvirus International Committee on Taxonomy of Viruses [ICTV]. (2022). Potyvirus - Potyviridae - Positive-sense RNA Viruses. https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/potyviridae/572/genus-potyvirus Rhoads, A. y Au, K. F. (2015). PacBio Sequencing and Its Applications. Genomics, Proteomics & Bioinformatics, 13(5), 278–289. https://doi:10.1016/j.gpb.2015.08.002 Riascos, D. (2011). Caracterización etiológica de la Roña de la gulupa (Passiflora edulis Sims.) en la región del Sumapaz (Cundinamarca). [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/9360 Rodríguez, M., Niño, N., Cutler, J., Langer, J., Casierra-Posada, F., Miranda, D., Bandte, M. y Büttner, C. (2016). Certificación de material vegetal sano en Colombia: un análisis crítico de oportunidades y retos para controlar enfermedades ocasionadas por virus. Revista Colombiana De Ciencias Hortícolas, 10(1), 164-175. https://doi.org/10.17584/rcch.2016v10i1.4921 Roingeard, P., Raynal, P. I., Eymieux, S. y Blanchard, E. (2019). Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety. Reviews in medical virology, 29(1). https://doi.org/10.1002/rmv.2019 Ryu, K. H. y Hong, J. S. (2008). Potexvirus. Encyclopedia of Virology, 310-313. https://doi:10.1016/b978-012374410-4.00738-x Sanger, F., Nicklen, S. y Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America, 74(12), 5463-5467. https://doi.org/10.1073/pnas.74.12.5463 Santillan, F. W., Fribourg, C. E., Adams, I. P., Gibbs, A. J., Boonham, N., Kehoe, M. A., Maina, S. y Jones, R. A. C. (2018). The Biology and Phylogenetics of Potato virus S Isolates from the Andean Region of South America. Plant Disease, 102(5), 869–885. https://doi.org/10.1094/PDIS-09-17-1414-RE Serrato, A., Flores, L., Aportela, J. y Sierra, E. (2014). PCR: reacción en cadena de la polimerasa. En: Herramientas moleculares aplicadas en ecología (pp. 53-73). http://www2.inecc.gob.mx/publicaciones2/libros/710/pcr.pdf Shaffer, L. (2019). Inner Workings: Portable DNA sequencer helps farmers stymie devastating viruses. Proceedings of the National Academy of Sciences, 116(9), 3351-3353. https://doi.org/10.1073/pnas.1901806116 Spadotti, D., Bello, V., Favara, G., Stangarlin, O., Krause-Sakate, R. y Rezende, J. (2019). Passiflora edulis: new natural host of Melochia yellow mosaic virus in Brazil. Australasian Plant Disease Notes, 14. https://doi.org/10.1007/s13314-019-0354-5 Taïwe, G. S. y Kuete, V. (2017). Passiflora edulis. Medicinal Spices and Vegetables from Africa, 513–526. http://doi:10.1016/b978-0-12-809286-6.00024-8 Tamayo, A. R. L., Betancur, C. E. y García, V. E. L. (2014). Manual técnico del cultivo de uchuva bajo buenas prácticas agrícolas. Torrance, L. (1998). Developments in serological methods to detect and identify plant viruses. Plant Cell, Tissue and Organ Culture, 52, 27-32. https://doi.org/10.1023/A:1005943823182 Torre, C. (2019). Detección y caracterización de virus epidemiológicamente relevantes en cultivos de tomate y cucurbitáceas [Tesis de doctorado, Universidad de Murcia]. http://hdl.handle.net/10201/74370 Trenado, H.P., Fortes, I.M. y Louro, D. (2007). Physalis ixocarpa and P. peruviana, new natural hosts of Tomato chlorosis virus. European Journal of Plant Pathology, 118, 193-196. https://doi.org/10.1007/s10658-007-9129-5 Vallejo, D. C., Gutiérrez, P. y Marín, M. (2016). Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja. Agronomía Colombiana, 34(1), 51-60. https://doi.org/10.15446/agron.colomb.v34n1.53161. Vidal, A.H., Sanches M.M., Alves-Freitas, D.M.T., Abreu, E.F.M., Lacorte, C., Pinheiro-Lima, B., Rosa, R.C.C., Jesus, O.N., Campos, M.A., Varsani, A. y Ribeiro S.G (2018). First World Report of Cucurbit aphid-borne yellows virus infecting Passionfruit. Plant Disease, 102(12): 2665. https://doi: 10.1094/PDIS-04-18-0694-PDN Villamor, D. E. V., Ho, T., Al Rwahnih, M., Martin, R. R. y Tzanetakis, I. (2019). High Throughput Sequencing in Plant Virus Detection and Discovery. Phytopathology. https://doi:10.1094/phyto-07-18-0257-rvw Vunsh, R., Rosner, A. y Stein, A. (1990). The use of the polymerase chain reaction (PCR) for the detection of bean yellow mosaic virus in gladiolus. Annals of Applied Biology, 117. https://doi.org/10.1111/j.1744-7348.1990.tb04822.x Wang, Q., Liu, Y., Xie, Y. y You, M. (2006). Cryotherapy of Potato Shoot Tips for Efficient Elimination of Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY). Potato Research, 49(2), 119-129. https://doi:10.1007/s11540-006-9011-4 Wang, Q., Cuellar, W., Rajamäki, M., Hirata, Y. y Valkonen, J. (2008).Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology, 9(2), 237-250. https://doi: 10.1111/j.1364-3703.2007.00456.x Wang, Q., Panis, B., Engelmann, F., Lambardi, M. y Valkonen J. (2009).Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation.Trends Plant Science, 14(3), 351-363. https://doi: 10.1016/j.tplants.2008.11.010. Wang, Y., Yang, Q. y Wang, Z. (2015). The evolution of nanopore sequencing. Frontiers in Genetics, 5, 449. https://doi.org/10.3389/fgene.2014.00449 Wang, M. R., Cui, Z. H., Li, J. W., Hao, X. Y., Zhao, L. y Wang, Q. C. (2018). In vitro thermotherapy-based methods for plant virus eradication. Plant Methods 14(1), 87. https://doi.org/10.1186/s13007-018-0355-y Wardrop, E. A., Gray, A. B., Singh, R. P. y Peterson, J. F. (1989). Aphid transmission of potato virus S. American Potato Journal, 66(8), 449-459. https://doi.org/10.1007/BF02855437 Waswa, M., Kakuhenzire, R. y Ochwo-Ssemakula, M. (2017). Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks. African Journal of Plant Science, 11(3), 61-70. https://doi.org/10.5897/AJPS2016.1497 Wylie, S. J. y Jones, M. G. K. (2010). The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses. Archives of Virology, 156(3), 479-482. https://doi:10.1007/s00705-010-0845-3 Zapata, J., Londoño, M., Diaz, Cipriano, A. y Saldarriaga, A. (2002). Manejo del cultivo de la uchuva en Colombia. AGROSAVIA. https://repository.agrosavia.co/handle/20.500.12324/12833?locale-attribute=es Zhang, S., Zhao, T., Liu, J., Zi, L., Li, X., Wang, Y., Zhang, Z., Li, D., Yu, J y Han, C. (2019). First Report of Cucurbit Aphid-Borne Yellows Virus in Passion fruit plants exhibiting mosaic and mottling in China. Plant Disease. https://doi:10.1094/pdis-07-19-1378-pdn Zibadi, S. y Watson, R. R. (2004). Passion Fruit (Passiflora edulis). Evidence-Based Integrative Medicine, 1(3), 183-187. https://doi:10.2165/01197065-200401030-00005 Al-Shahwan, I. M., Abdalla, O. A., Al-Saleh, M. A. y Amer, M. A. (2017). Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi J. Biol. Sci. 24(6), 1336-1343. https://doi.org/ 10.1016/j.sjbs.2016.02.022. Anwar, I., Bukhari, H. A., Nahid, N., Rashid, K., Amin, I., Shaheen, S., Hussain, K. y Mansoor, S. (2020). Association of cotton leaf curl Multan betasatellite and Ageratum conyzoides symptomless alphasatellite with tomato leaf curl New Delhi virus in Luffa cylindrica in Pakistan. Australasian Plant Pathology, 49(1), 25–29. https://doi.org/10.1007/s13313-019-00668-6 Cámara de comercio de Bogotá. (2015). Manual Gulupa. http://hdl.handle.net/11520/14314 Chen, G., Pan, H., Xie, W., Wang, S., Wu, Q., Fang, Y., Shi, X. y Zhang, Y. (2013). Virus infection of a weed increases vector attraction to and vector fitness on the weed. Scientific Reports, 3(1), 2253. https://doi.org/10.1038/srep02253 Cheng, T., Xu, C., Lei, L., Li, C., Zhang, Y. y Zhou, S. (2016), Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity. Molecular Ecology Resources, 16, 138-149. https://doi.org/10.1111/1755-0998.12438 Cutler, J., Langer, J., Von, S., Acosta, O., Casierra, F., Castañeda, A., Betancourt, M., Cuellar, W., Arvydas, E., Altenbach, D. y Büttner, C. (2018). Preliminary evaluation of associated viruses in production systems of cape gooseberry, purple passion fruit, and rose. Revista Colombiana de Ciencias Hortícolas, 12(2), 390-396. https://doi:10.17584/rcch.2018vl2i2.7799 Cuspoca, J. (2007). Evaluación de virus de tomate de árbol (Solanum betaceum) en plantas indicadoras y su detección por PCR. Tesis en agronomia. Facultad de agronomia. Universidad Nacional de Colombia. Bogotá. 31 p. DANE. (2020). Exportaciones-Histórico. https://www.dane.gov.co/index.php/estadisticas-por-tema/comercio-internacional/exportaciones/exportaciones-historicos Doyle, J. (1991). DNA Protocols for Plants. Molecular Techniques in Taxonomy. Springer. https://doi:10.1007/978-3-642-83962-7_18 Duque, M., Marín, M. y Gutiérrez, P.A. (2017). Genome comparison and primer design for detection of Tamarillo leaf malformation virus (TaLMV). Archives of Phytopathology and Plant Protection, 50, 713-726. https://doi.org/10.1080/03235408.2017.1370934 EPPO. (2021). Galinsoga quadriradiata. https://gd.eppo.int/taxon/GASCI/pests Feng, J.L., Chen, S.N., Tang, X.S., Ding, X.F., Du, Z.Y. y Chen, J.S. (2006). Quantitative determination of cucumber mosaic virus genome RNAs in virions by Real-Time Reverse Transcription-Polymerase Chain Reaction. Acta Biochimica et Biophysica Sinica, 38(10): 669-676. https://doi.org/10.1111/j.1745-7270.2006.00216.x Gao, L. X., Ding, K., Li, W., Liao, Y., Zhong, R., Ren, Z., Liu, K., Adhimoolam, K. y H. Zhi. (2015). Characterization of Soybean mosaic virus resistance derived from inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars. Theoretical and Applied Genetics 128, 1489-1505. https://doi.org/10.1007/s00122-015-2522-0 Hebert, P. D., Cywinska, A., Ball, S. L. y deWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings. Biological sciences, 270(1512), 313–321. https://doi.org/10.1098/rspb.2002.2218 Hebert, P. D. N., Penton, E. H., Burns, J. M., Janzen, D. H. y Hallwachs, W. (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences, 101(41), 14812–14817. doi:10.1073/pnas.0406166101 Hobbs, H. A., Eastburn, D. M., D’Arcy, C. J., Kindhart, J. D., Masiunas, J. B., Voegtlin, D. J., Weinzierl, R. A. y McCoppin, N. K. (2000). Solanaceous Weeds as Possible Sources of Cucumber mosaic virus in Southern Illinois for Aphid Transmission to Pepper. Plant Disease, 84(11), 1221–1224. https://doi.org/10.1094/PDIS.2000.84.11.1221 Huang, C. J., Liu, Y., Yu, H. Q., Liu, B. Z., y Qing, L. (2016). Bidens pilosa is a Natural Host of Tomato spotted wilt virus in Yunnan Province, China. Plant Disease, 100(9), 1957. https://doi.org/10.1094/PDIS-03-16-0344-PDN Hull, R. (2013). Plant Virology. Fifth Edition. Academic Press. Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2018). Molecular characterization of Soybean mosaic virus (SMV) infecting Purple passion fruit (Passiflora edulis f. edulis) in Antioquia, Colombia. Archives of Phytopathology and Plant Protection, 1–20. https://doi:10.1080/03235408.2018.1505411 Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2019). Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passion fruit (Passiflora edulis f. edulis). Revista Facultad Nacional de Agronomia Medellín, 72(1), 8643-8654. https://doi.org/10.15446/rfnam.v72n1.69438 Junco, M.C., Silva, C.D.C., do Carmo, C.M., Kotsubo, R.Y., de Novaes, T.G. y Molina, R.D.O. (2020). Identification of potential hosts plants of Cowpea aphid-borne mosaic virus. Journal of Phytopathology. 169, 45– 51. https://doi.org/10.1111/jph.12957 Kamaal, N., Akram, M., Gupta, S. y Agnihotri, A. (2014). Ageratum conyzoides Harbours Mungbean yellow mosaic India virus. Plant Pathology Journal, 13. https://doi.org/10.3923/ppj.2014.59.64 Kaliciak, A. y Syller, J. (2009). New hosts of Potato virus Y (PVY) among common wild plants in Europe. European Journal of Plant Pathology124, 707–713 https://doi.org/10.1007/s10658-009-9452-0 Kashina, B., MABAGALA, B. y Mpunami, A. (2003). First Report of Ageratum conyzoides L. and Sida acuta Burm F. as New Weed Hosts of Tomato Yellow Leaf Curl. Plant Protection Science, 39. https://doi.org/10.17221/3822-PPS Kumar, S., Stecher, G., Li, M., Knyaz, C. y Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096 Marín, M. y Gutiérrez, P.A. (2016). Principios de virología molecular de plantas tropicales. Agrosavia. http://editorial.agrosavia.co/index.php/publicaciones/catalog/book/69 Miranda, D., G. Fischer, C. Carranza, S. Magnitskiy, F. Casierra-Posada, W. Piedrahíta y Flórez, L. (2009). Cultivo, poscosecha y comercialización de las pasifloráceas en Colombia: maracuyá, granadilla, gulupa y curuba. Sociedad Colombiana de Ciencias Hortícolas, Bogotá.http://www.asohofrucol.com.co/archivos/biblioteca/biblioteca_118_cultivo_poscosechavp.pdf Miranda, D., Carranza Gutiérrez, C. E., Fischer Gebauer, G., Jerez, C., Ramírez Godoy, A., Forero de la Rotta, M. C., Quevedo, K., Lanchero, O., Obregón Corredor, D., Chaves Córdoba, B., Pedraza Flautero, M., Plaza Trujillo, G. A., Martínez Maldonado, F. E. y Torres Arango, C. (2016). Problemas de campo asociados al cultivo de uchuva (Physalis peruviana L.) (Primera edición). Universidad Nacional de Colombia. Facultad de Ciencias Agrarias. http://ezproxy.unal.edu.co/login?url=https://search.ebscohost.com/login.aspx?direct=true&db=cat02704a&AN=unc.000836877&lang=es&site=eds-live Plaza, G. A. y Pedraza, M. (2007). Reconocimiento y caracterización ecológica de la flora arvense asociada al cultivo de uchuva. Agronomía Colombiana, 25, 306–313. Pandey, N., Tiwari, A., Rao, G. y Shukla, K. (2011). Detection and identification of Ageratum enation virus infecting Ageratum conyzoides in India. Acta Phytopathologica et Entomologica Hungarica, 46(2), 203–211. https://doi.org/10.1556/aphyt.46.2011.2.4 Rojas, J. (2020). Bidens pilosa (blackjack). Invasive Species Compendium. CABI. https://doi:10.1079/ISC.9148.20203483381 Roossinck, M.J., Saha, P., Wiley, G.B., Quan, J., White, J.D., Lai, H. et al. (2010) Ecogenomics: using massively parallel pyrosequencing to understand virus ecology. Molecular Ecology, 19, 81–88. https://doi.org/10.1111/j.1365-294X.2009.04470.x Salazar, L. F., Muller, G., Querci, M., Zapata, J. L. y Owens, R. A. (2000). Potato yellow vein virus: its host range, distribution in South America and identification as a crinivirus transmitted by Trialeurodes vaporariorum. Annals of Applied Biology, 137(1), 7–19. https://doi.org/https://doi.org/10.1111/j.1744-7348.2000.tb00052.x Savenkov, E.I., Sandgren, M.Y. y Valkonen, J.P.T. (1999). Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus. Journal of General Virology, 80, 2779-2784. https://10.1099/0022-1317-80-10-2779 Schena, L., Nigro, F., Ippolito, A. y Gallitelli, D. (2004). Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. European Journal of Plant Pathology, 110(9), 893-908. https://doi.org/10.1007/s10658-004-4842-9 Shibuya, Y., Sakata, J., Sukamto, N., Kon, T., Sharma, P. y Ikegami, M. (2007). First Report of Pepper yellow leaf curl Indonesia virus in Ageratum conyzoides in Indonesia. Plant Disease, 91(9), 1198. https://doi.org/10.1094/PDIS-91-9-1198B Singh, M., Singh, R.P., Fageria, M.S., Nie, X., Coffin, R. y Hawkins, G. (2013). Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers. American Journal of Potato Research, 90, 43–50. https://doi.org/10.1007/s12230-012-9274-z Singh, G., Singh, N. P. y Singh, R. (2014). Food plants of a major agricultural pest Aphis gossypii Glover (Homoptera: Aphididae) from India: An updated checklist. International Journal of Research Studies in Biosciences, 3, 1-26. Tan, H. N. P. y Wong, S. M. (1993). Some Properties of Singapore Ageratum Yellow Vein Virus (SAYVV). Journal of Phytopathology, 139(2), 165–176. https://doi.org/https://doi.org/10.1111/j.1439-0434.1993.tb01412.x Wamonje, F. O., Tungadi, T. D., Murphy, A. M., Pate, A. E., Woodcock, C., Caulfield, J. C., Mutuku, J. M., Cunniffe, N. J., Bruce, T. J. A., Gilligan, C. A., Pickett, J. A. y Carr, J. P. (2020). Three Aphid-Transmitted Viruses Encourage Vector Migration From Infected Common Bean (Phaseolus vulgaris) Plants Through a Combination of Volatile and Surface Cues. Frontiers in Plant Science, 11. https://www.frontiersin.org/article/10.3389/fpls.2020.613772 Xanthis, C.K., Maliogka, V.I., Lecoq, H., Dezbiez, C., Tsvetkov, I. y Katis, N.I. (2015). First report of cucumber mosaic virus infecting watermelon in Greece and Bulgaria, Journal of Plant Pathology, 97(2), 391-403. http://dx.doi.org/10.4454/JPP.V97I2.007 Yazdkhasti, E., Hopkins, R. y Kvarnheden, A. (2021). Reservoirs of Plant Virus Disease: Occurrence of Wheat Dwarf Virus and Barley/Cereal Yellow Dwarf Viruses in Sweden. Plant Pathol. 70, 1552–1561. https://doi.org/10.1111/ppa.13414 Yu, J., Xue, J.-H. y Zhou, S.-L. (2011). New universal matK primers for DNA barcoding angiosperms. Journal of Systematics and Evolution, 49, 176-181. https://doi.org/10.1111/j.1759-6831.2011.00134.x Zapata, J., Londoño, M., Diaz, Cipriano, A. y Saldarriaga, A. (2002). Manejo del cultivo de la uchuva en Colombia. AGROSAVIA. https://repository.agrosavia.co/handle/20.500.12324/12833?locale-attribute=es AGUILERA P., TACHIQUIN M., ROCHA M.G., PINEDA B. 2014. PCR en tiempo real. Herramientas moleculares aplicadas en ecología: aspectos teóricos y prácticos. 175–201. GARCÍA N., GUTIÉRREZ P.A, MARÍN M.M. 2013. Detección y cuantificación del Potato mop-top virus (PMTV) en Colombia mediante qRT-PCR. Acta Agronómica.62(2):120-128. NIE X & SINGH R.P. 2001. A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves and tubers. Journal of Virological Methods.91:37–49. https://10.1016/s0166-0934(00)00242-1. PÁSSARO, C. (Ed). 2014. Physalis peruviana L: fruta andina para el mundo. http://www.cyted.org/es/noticias/libro-physalis-peruviana-l-fruta-andina-para-el-mundo SAMBROOK J., & RUSSELL, D. 2001. Molecular Cloning: A Laboratory Manual (3.a ed.). Spring Harbor Laboratory Press. SCHENA L., NIGRO F., IPPOLITO A., GALLITELLI D. 2004. Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. European Journal of Plant Pathology.110(9):893-908. https://doi.org/10.1007/s10658-004-4842-9 SHIRIMA R. R., MAEDA D. G., KANJU E., CEASAR G., TIBAZARWA F. I., LEGG J. P. 2017. Absolute quantification of cassava brown streak virus mRNA by real-time qPCR. Journal of Virological Methods.245:5-13. https://doi:10.1016/j.jviromet.2017.03.003 WONG M. L. Y MEDRANO J. F. 2005. Real-time PCR for mRNA quantitation. BioTechniques.39(1):75-85. https://doi:10.2144/05391rv01 Andrade, S., Fosneca, L., Silva, M., Faleiro, F. y Junqueira, N. (2010). Estudos Preliminares para o Uso de Termoterapia ex vitro em Maracujazeiro-Azedo visando à Eliminação de Vírus-do- endurecimento-dos-frutos. Boletim de Pesquisa e Desenvolvimento, 267 Balamuralikrishnan, M., Doraisamy, S., Ganapathy, T. y Viswanathan, R. (2002). Combined effect of chemotherapy and meristem culture on sugarcane mosaic virus elimination in sugarcane. Sugar Tech, 4(1-2), 19–25. https://doi:10.1007/bf02956875 Cutler, J., Langer, J., Von, S., Acosta, O., Casierra, F., Castañeda, A., Betancourt, M., Cuellar, W., Arvydas, E., Altenbach, D. y Büttner, C. (2018). Preliminary evaluation of associated viruses in production systems of cape gooseberry, purple passion fruit, and rose. Revista Colombiana de Ciencias Hortícolas, 12(2), 390-396. https://doi:10.17584/rcch.2018vl2i2.7799 Duque, M., Marín, M. y Gutiérrez, P.A. (2017). Genome comparison and primer design for detection of Tamarillo leaf malformation virus (TaLMV). Archives of Phytopathology and Plant Protection, 50, 713-726. https://doi.org/10.1080/03235408.2017.1370934 Faccioli G. (2001). Control de virus de la papa mediante cultivos de meristemo y corte de tallo, termoterapia y quimioterapia. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0842-6_28 Gil, J. F., Adams, I., Boonham, N., Nielsen, S. L. y Nicolaisen, M. (2016). Molecular and biological characterization of Potato mop-top virus (PMTV, Pomovirus) isolates from the potato-growing regions of Colombia. Plant Pathology, 65(7), 1210–1220. https://doi.org/10.1111/ppa.12491 Gómez, J.E., F. Morales, J. Arroyave. (1997). Mosaic disease of Physalis peruviana in Colombia. ASCOLFI Informa. 23:52. Gong, H., Igiraneza, C. y Dusengemungu, L. (2019). Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review. American Journal of Potato Research. 96, 379-389. https://doi.org/10.1007/s12230-019-09720-z Grout, B. W. W. (1999). Meristem-Tip Culture for Propagation and Virus Elimination. Plant Cell Culture Protocols, 115-126. https://doi.org/10.1385/1-59259-583-9:115 Hansen, A. J. y Lane, W. D. (1985). Elimination of apple chlorotic leafspot virus from apple shoot cultures by ribavirin. Plant Disease, 69(2), 134-135. Heredia, S. C. (2016). Erradicación de Onion Yellow Dwarf Virus (OYDV) en cebolla Shallot (Allium cepa var. Aggregatum), mediante el cultivo de meristemas, quimioterapia y termoterapia para la producción de bulbos libres de virus [Tesis de pregrado, Universidad San Francisco de Quito]. Repositorio Digital USFQ. http://repositorio.usfq.edu.ec/handle/23000/5198 Mellor, F. C. y Stace, R. (1987). Virus-Free Potatoes Through Meristem Culture. Potato, 30-39. https://doi:10.1007/978-3-642-72773-3_3 Montiel , O., Pastelín, M., Ventura, E., Castañeda, O., Gonzalez, M. y Guevara, M. (2011). Tropical and subtropical agroecosystem, 13(3), 537-542. Nasir, I., y Husnain, T. (2010). Strategies to control Potato Virus Y under in vitro conditions. Pakistan Journal Phytopathology, 22, 63–70. Pennazio, S. y Appiano, A. (1975). Potato virus X aggregates in ultrathin sections of Datura meristematic dome. Phytopathologia Mediterranea, 14(1), 12-15. http://www.jstor.org/stable/42684242 Savenkov, E.I., Sandgren, M.Y. y Valkonen, J.P.T. (1999). Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus. Journal of General Virology, 80, 2779-2784. https://10.1099/0022-1317-80-10-2779 Simpkins, I., Walkey, D. G. A. y Neely, H. A. (1981). Chemical suppression of virus in cultured plant tissues. Annals of Applied Biology, 99(2), 161-169. https://doi.org/10.1111/j.1744-7348.1981.tb05143.x Vallejo, D., Gutiérrez, P. y M, Marín. (2016). Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk. Agronomía Colombiana 34, 51–60. https://doi.org/10.15446/agron.colomb.v34n1.53161. Singh, B. (2015). Effect of antiviral chemicals on in vitro regeneration response and production of PLRV-free plants of potato. Journal of Crop Science and Biotechnology, 18(5), 341-348. https://doi.org/10.1007/s12892-015-0069-x Wang, Q., Panis B, Engelmann, F., Lambardi, M. y Valkonen, J. (2009). Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation.Trends Plant Science, 14(3), 351-363. https://doi.org/10.1111/j.1744-7348.2008.00308.x Wang, M. R., Cui, Z. H., Li, J. W., Hao, X. Y., Zhao, L. y Wang, Q. C. (2018). In vitro thermotherapy-based methods for plant virus eradication. Plant Methods 14(1), 87. https://doi.org/10.1186/s13007-018-0355-y Zhang, Z., Wang, Q.C., Spetz, C. y Blystad, D.R. (2019). In vitro therapies for virus elimination of potato-valuable germplasm in Norway. Scientia Horticulturae, 249, 7-14. https://doi.org/10.1016/j.scienta.2019.01.027 Fuchs, M., C. Schmitt-Keichinger, H. Sanfaçon. 2017. A renaissance in Nepovirus research provides new insights into their molecular interface with hosts and vectors. Advances in Virus Research 97(61-105). https://doi.org/10.1016/bs.aivir.2016.08.009 Fischer, Gerhard., Almanza-Merchán., Pedro José. y Miranda, Diego. (2014). Importancia y cultivo de la uchuva (Physalis peruviana L.). Revista Brasileira de Fruticultura, 36(1), 01-15. https://dx.doi.org/10.1590/0100-2945-441/13 Gong, H., Igiraneza, C. y Dusengemungu, L. (2019). Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review. American Journal of Potato Research. 96, 379-389. https://doi.org/10.1007/s12230-019-09720-z Hobbs, H. A., Eastburn, D. M., D’Arcy, C. J., Kindhart, J. D., Masiunas, J. B., Voegtlin, D. J., Weinzierl, R. A. y McCoppin, N. K. (2000). Solanaceous Weeds as Possible Sources of Cucumber mosaic virus in Southern Illinois for Aphid Transmission to Pepper. Plant Disease, 84(11), 1221–1224. https://doi.org/10.1094/PDIS.2000.84.11.1221 Johansen, E., M.C. Edwards, R.O. Hampton. 1994. Seed transmission of viruses: Current King AMQ (2012). Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego. Pallas, V., F. Aparicio, M.C. Herranz, K. Amari, M.A. Sanchez-Pina, A. Myrta, J.A. Sanchez-Navarro. 2012. Ilarviruses of Prunus spp.: a continued concern for fruit trees. Phytopathology 102(12), 1108-20. https://10.1094/PHYTO-02-12-0023-RVW Plaza, G. A. y Pedraza, M. (2007). Reconocimiento y caracterización ecológica de la flora arvense asociada al cultivo de uchuva. Agronomía Colombiana, 25, 306–313. Mellor, F. C. y Stace, R. (1987). Virus-Free Potatoes Through Meristem Culture. Potato, 30-39. https://doi:10.1007/978-3-642-72773-3_3 Wamonje, F. O., Tungadi, T. D., Murphy, A. M., Pate, A. E., Woodcock, C., Caulfield, J. C., Mutuku, J. M., Cunniffe, N. J., Bruce, T. J. A., Gilligan, C. A., Pickett, J. A. y Carr, J. P. (2020). Three Aphid-Transmitted Viruses Encourage Vector Migration From Infected Common Bean (Phaseolus vulgaris) Plants Through a Combination of Volatile and Surface Cues. Frontiers in Plant Science, 11. https://www.frontiersin.org/article/10.3389/fpls.2020.613772 Agindotan, B. O., Shiel, P. J., y Berger, P. H. (2007). Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan® real-time RT-PCR. Journal of Virological Methods, 142(1-2), 1-9. http://doi:10.1016/j.jviromet.2006.12.012 Agronet. (2018). Reporte: Área, Producción y Rendimiento Nacional por Cultivo. https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1 Almanza, P.J. (2000). Propagación. En, Flórez, V.J., Fischer, G. y Sora, A.D (Eds.). Producción, poscosecha y exportación de la uchuva (Physalis peruviana L.) (pp. 27–40). Universidad Nacional de Colombia, Bogotá. https://repository.agrosavia.co/handle/20.500.12324/30703 AlMaarri, K., Massa, R. y AlBiski, F. (2012). Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants. Plant Biotechnology, 29(3), 237-243. http://doi:10.5511/plantbiotechnology.12.0215a Álvarez, N., Jaramillo, H., Gallo, Y., Gutiérrez, P. y Marín, M. (2018). Molecular characterization of Potato virus Y (PVY) and Potato virus V (PVV) isolates naturally infecting cape gooseberry (Physalis peruviana) in Antioquia, Colombia. Agronomía Colombiana, 36(1), 13-23. https://dx.doi.org/10.15446/agron.colomb.v36n1.65051 Analdex. (2020). Exportación de Gulupa en 2020. https://www.analdex.org/2021/02/25/exportacion-de-gulupa-en-2020/ Analdex. (2021). Informe exportaciones de uchuva. https://www.analdex.org/2021/10/20/informe-exportaciones-de-uchuva/ Analdex. (s.f.). ¡Uchuva / Goldenberry nuestra Embajadora de frutas Colombianas para el mundo!. https://www.analdex.org/2020/06/30/uchuva-goldenberry-nuestra-embajadora-de-frutas-colombianas-para-el-mundo/ Andrade, S., Fosneca, L., Silva, M., Faleiro, F. y Junqueira, N. (2010). Estudos Preliminares para o Uso de Termoterapia ex vitro em Maracujazeiro-Azedo visando à Eliminação de Vírus-do- endurecimento-dos-frutos. Boletim de Pesquisa e Desenvolvimento, 267. Ateş, S.Y., İnan, S., Ayyaz, M., Dündar, I. y Yabangülü, D. (2019). Effect of thermotherapy in combination with meristem culture for eliminating potato virus Y (PVY) and potato virus S (PVS) from infected seed stocks. The Journal of Animal & Plant Sciences, 29(2), 549-555. http://www.thejaps.org.pk/docs/V-29-02/26.pdf Bang, B., Lee, J., Kim, S., Park, J., Nguyen, T. T. y Seo, Y. S. (2014). A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus. The Plant Pathology Journal, 30(3), 310-315. https://doi.org/10.5423/PPJ.NT.03.2014.0025 Bartholomaus, A., A. de la Rosa, J.O. Santos, L.E. Acero y W, Moosbrugger. (1990). Physalis peruviana. El manto de la tierra - Flora de los Andes. Guía de 150 especies de la flora andina. (pp. 163-164). Lerner, Bogotá. Benscher, D., Pappu, S.S., Niblett, C.L., Varón de Agudelo, F., Morales, F., Hodson, E., Álvarez, E., Acosta, O. y Lee, R.F. (1996). A strain of Soybean mosaic virus infecting Passiflora spp. In Colombia. Plant Disease. 80, 258-262. http://doi: 10.1094/PD-80-0258. Bhat, A.I. y Rao, G.P. (2020). Rolling Circle Amplification (RCA). En, Characterization of Plant Viruses (pp. 377-381). Springer Protocols Handbooks.https://doi.org/10.1007/978-1-0716-0334-5_2 Blanco, J. (2000). Manejo de enfermedades. En, V. J., Flóres, G., Fischer, Á. D., Sora, (Eds). Cultivos de uchuva. Producción, pos-cosecha y exportación de la uchuva (Physalis peruviana L.) (pp. 57-65). Universidad Nacional de Colombia. Blawid, R., Silva, J. M. F. y Nagata, T. (2017). Discovering and sequencing new plant viral genomes by next-generation sequencing: description of a practical pipeline. Annals of Applied Biology, 170(3), 301–314. http://doi:10.1111/aab.12345 Bolger, A. M., Lohse, M. y Usadel, B. (2014). Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics (Oxford, England), 30(15), 2114-2120. https://doi.org/10.1093/bioinformatics/btu170 Boonham, N., Laurenson, L., Weekes, R. y Mumford, R. (2008). Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR. Methods in Molecular Biology, 249-258. http://doi:10.1007/978-1-59745-062-1_19 Boonham, N., Kreuze, J., Winter, S., van der Vlugt, R., Bergervoet, J., Tomlinson, J. y Mumford, R. (2014). Methods in virus diagnostics: From ELISA to next generation sequencing. Virus Research, 186, 20-31. http://doi:10.1016/j.virusres.2013.12.007 Boratyn, G. M., Thierry-Mieg, J., Thierry-Mieg, D., Busby, B. y Madden, T. L. (2019). Magic-BLAST, an accurate RNA-seq aligner for long and short reads. BMC Bioinformatics, 20(1). http:// doi:10.1186/s12859-019-2996-x Camelo, V.M. (2010). Detección e identificación de los virus patógenos de cultivos de gulupa (Passiflora edulis Sims.) en la región de Sumapaz (Cundinamarca). [Tesis de Maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/6860 Cámara de comercio de Bogotá. Manual Gulupa. (2015). https://bibliotecadigital.ccb.org.co/bitstream/handle/11520/14314/Gulupa.pdf?sequence=1&isAllowed=y Chen, S., Yu, N., Yang, S. Zhong, B.y Lan, H. (2018). Identification of Telosma mosaic virus infection in Passiflora edulis and its impact on phytochemical contents Virology Jurnal 15, 168. https://doi.org/10.1186/s12985-018-1084-6 Chiemsombat, P., Prammanee, S. y Pipattanawong, N. (2014). Occurrence of Telosma mosaic virus causing passion fruit severe mosaic disease in Thailand and immunostrip test for rapid virus detection. Crop Protection, 63, 41–47. https://doi.org/https://doi.org/10.1016/j.cropro.2014.04.023 Chong, YH., Cheng, YH., Cheng, HW. (2018). The virus causing passionfruit woodiness disease in Taiwan is reclassified as East Asian passiflora virus. Journal of General Plant Pathology 84, 208-220. https://doi.org/10.1007/s10327-018-0777-4 Clark, M.F. y A.N. Adams. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34(3), 475-483. https://doi.org/10.1099/0022-1317-34-3-475 Corrales-Cabra, E., Higuita, M., Hoyos, R., Gallo, Y., Marín, M. y Gutiérrez, P. (2021). Prevalence of RNA viruses in seeds, plantlets, and adult plants of cape gooseberry (Physalis peruviana) in Antioquia (Colombia). Physiological and Molecular Plant Pathology 116, 101715. https://doi.org/10.1016/j.pmpp.2021.101715. Corrales-Cabra, E. (2022). Caracterización genómica de los virus que infectan los cultivos de uchuva (Physalis peruviana) en Antioquia para el apoyo de los programas de certificación de semilla [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/82181 Crestani, O.A., Kitajima, E.W., Lin, M.T. y Marinho V.L. (1986). Passion Fruit Yellow Mosaic Virus, a New Tymovirus Found in Brazil. The American Phytopathological Society 76(9), 951-955. https://doi:10.1094 / Phyto-76-951 Cutler, J., Langer, J., Von, S., Acosta, O., Casierra, F., Castañeda, A., Betancourt, M., Cuellar, W., Arvydas, E., Altenbach, D. y Büttner, C. (2018). Preliminary evaluation of associated viruses in production systems of cape gooseberry, purple passion fruit, and rose. Revista Colombiana de Ciencias Hortícolas, 12(2), 390-396. https://doi:10.17584/rcch.2018vl2i2.7799 DANE. (2020). Exportaciones-Histórico. https://www.dane.gov.co/index.php/estadisticas-por-tema/comercio-internacional/exportaciones/exportaciones-historicos D'Arcy, W. (1991). The Solanaceae since 1976, with a review of its biogeography. En: Hawkes, J., Lester, R.L., Nee, M. y Estrada N (Eds.), Solanaceae: Taxonomy, chemistry, evolution, 75-137. Davis, J. A., Radcliffe, E. B. y Ragsdale, D. W. (2005). Soybean aphid, Aphis glycines Matsumura, a new vector of Potato virus Y in potato. American Journal of Potato Research, 82(3), 197-201. https://doi:10.1007/bf02853585. Daza, P.A. y Rodríguez, P.A. (2006). Enfermedades de origen viral en plantas de uchuva (Physalis peruviana L.) en el Departamento de Cundinamarca. [Tesis de pregrado, Pontificia Universidad Javeriana] Bogotá. Díaz, A., Quiñones, M., Arana, F., Soto, M. y Hernández, Annia. (2010). Potyvirus: características generales, situación de su diagnóstico y determinación de su presencia en el cultivo del pimiento en Cuba. Revista de Protección Vegetal, 25(2), 69-79. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522010000200001&lng=es&tlng=. Domier, L. L., Hobbs, H. A., McCoppin, N. K., Bowen, C. R., Steinlage, T. A., Chang, S., Wang, Y. y Hartman, G. L. (2011). Multiple loci condition seed transmission of soybean mosaic virus (SMV) and SMV-induced seed coat mottling in soybean. Phytopathology, 101(6), 750–756. https://doi.org/10.1094/PHYTO-09-10-0239. Eiras, M., Costa, I.F.D., Chaves, A.L.R., Colariccio, A., Harakava, R., Tanaka, F.A.O., Garcêz, R.M. y Silva, L.A. (2012). First report of a tospovirus in a commercial crop of Cape gooseberry in Brazil. New Disease Reports 25, 25. http://dx.doi.org/10.5197/j.2044-0588.2012.025.025 Esquivel, A. F., Rezende, J. A. M., Lima, E. F. B., Kitajima, E. W. y Diniz, F. O. (2018). First Report of Groundnut ring spot virus on Physalis peruviana in Brazil. Plant Disease, 102(7), 1468–1468. http://doi:10.1094/pdis-10-17-1684-pdn Fariña, A.E., Gorayeb, E.S. y Camelo-García., V.M. (2019). Caracterización molecular y biológica de un nuevo sobemovirus putativo que infecta a Physalis peruviana. Archives Virology 164, 2805–2810.https://doi.org/10.1007/s00705-019-04374-y Fereres, A., Perez, P., Gemeno, C. y Ponz, F. (1993). Transmission of Spanish Pepper- and Potato-PVY Isolates by Aphid (Homoptera: Aphididae) Vectors: Epidemiological Implications. Environmental Entomology, 22(6), 1260-1265. https://doi.org/10.1093/ee/22.6.1260 Fisher, G., Miranda, D., Romero, J. y Piedrahita, W. (Eds). (2005). Avances en cultivo poscosecha y exportación de la uchuva (Physalis peruviana) en Colombia. Universidad Nacional de Colombia.http://www.asohofrucol.com.co/archivos/biblioteca/biblioteca_23_Avances%20cultivo%20uchuva.pdf Fischer, I.H. y Rezende, J.A.M. (2008). Diseases of Passion Flower (Passiflora spp.). Pest Technology 2(1): 1-19. https://www.researchgate.net/publication/228483390_Diseases_of_Passion_flower_Passiflora_spp Fischer, G. y Miranda, D. (Eds) (2012). Uchuva (Physalis peruviana L.). Manual para el cultivo de frutas tropicales en el trópico. Produmedios. Fontenele, R. S., Lamas, N. S., Lacorte, C., Lacerda, A. L. M., Varsani, A. y Ribeiro, S. G. (2017). A novel geminivirus identified in tomato and cleome plants sampled in Brazil. Virus Research, 240, 175-179. https://doi:10.1016/j.virusres.2017.08.007 Freedman, A. y Weeks, N. (2020). Best Practices for De Novo Transcriptome Assembly with Trinity. Hardvard FAS Informatics. https://informatics.fas.harvard.edu/best-practices-for-de-novo-transcriptome-assembly-with-trinity.html Fribourg, C.E. (1979). Host plant reactions, some properties, and serology of Peru tomato virus. Phytopathology. 69(5), 441–445. Fribourg, C. E. (1987). A New Tobamovirus from Passiflora edulis in Peru. Phytopathology, 77(3), 486. https://doi.org/10.1094/phyto-77-486 Fukumoto, T., Nakamura, M. y Rikitake, M. (2012). Molecular characterization and specific detection of two genetically distinguishable strains of East Asian Passiflora virus (EAPV) and their distribution in southern Japan. Virus Genes, 44, 141-148. https://doi.org/10.1007/s11262-011-0676-7 Gallo, Y. M, Jaramillo, H., Toro, L. F., Marín, M. y Gutiérrez, P. A. (2018). Characterization of the genome of a novel ilarvirus naturally infecting Cape gooseberry (Physalis peruviana). Archives of Virology, 163(6), 1713-1716. https://doi.org/10.1007/s00705-018-3796-8 Gallo, Y., Montoya, M. M. y Gutiérrez, P. A. (2020). Detection of RNA viruses in Cape gooseberry (Physalis peruviana L.) by RNAseq using total RNA and dsRNA inputs. Archives of Phytopathology and Plant Protection, 53(9-10), 395-413. https://doi.org/10.1080/03235408.2020.1748368 Geering, A. D. W. (2021). Badnaviruses (Caulimoviridae) (D. H. Bamford & M. B. T.-E. of V. (Fourth E. Zuckerman (eds.); pp. 158–168). Academic Press. https://doi.org/https://doi.org/10.1016/B978-0-12-814515-9.00147-8 Geniza, M. y Jaiswal, P. (2017). Tools for building de novo transcriptome assembly. Current Plant Biology, 11-12, 41-45. https://doi:10.1016/j.cpb.2017.12.004 Gergerich, R.C. y Dolja, V. V. (2006). Introducción a los virus vegetales, el enemigo invisible. The Plant Health Instructor. https://doi:10.1094/PHI-I-2008-0122-01 Gildow, F. E., Shah, D. A., Sackett, W. M., Butzler, T., Nault, B. A. y Fleischer, S. J. (2008). Transmission efficiency of Cucumber mosaic virus by aphids associated with virus epidemics in snap bean. Phytopathology, 98(11), 1233-1241. https://doi.org/10.1094/PHYTO-98-11-1233 Goodwin, S., McPherson, J. D. y McCombie, W. R. (2016). Coming of age: ten years of next-generation sequencing technologies. Nature Reviews Genetics, 17(6), 333–351. https://doi:10.1038/nrg.2016.49 Gómez, P., Sempere, R. y Aranda, M. A. (2012). Pepino Mosaic Virus and Tomato Torrado Virus. Viruses and Virus Diseases of Vegetables in the Mediterranean Basin, 505–532. https://doi:10.1016/b978-0-12-394314-9.00014-2 González-Garza, R. (2017). Evolución de técnicas de diagnóstico de virus fitopatógenos. Revista Mexicana de fitopatología, 35(3), 591-610. https://doi.org/10.18781/r.mex.fit.1706-1 Gordillo, L.A. (2011). Incidencia del Soybean mosaic virus en cultivos de Gulupa (Passiflora edulis Sims) en Cundinamarca y estudio de su diversidad en Colombia. [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/9885 Graça, J. V., Trench, T. N. y Martin, M. M. (1985). Tomato spotted wilt virus in commercial Cape gooseberry (Physalis peruviana) in Transkei. Plant Pathology, 34(3), 451–453. https://doi:10.1111/j.1365-3059.1985.tb01390.x Green, K.J., Chikh-Ali, M., Hamasaki, R.T., Melzer, M.J. y Karasev, A.V.(2017). Potato virus Y (PVY) Isolates from Physalis peruviana are unable to systemically infect Potato or Pepper and form a distinct new lineage Within the PVYC Strain Group. Phytopathology, 107(11):1433-1439. https://doi:10.1094/PHYTO-04-17-0147-R Gupta, S.P. y Singh, B.R. (1996). Severe mosaic of Cape gooseberry due to Cucumber mosaic virus. Indian Journal Virology, 24(1), 27-156 Hajimorad, M. R., Domier, L. L., Tolin, S. A., Whitham, S. A. y Saghai, M. A. (2018). Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range. Molecular Plant Pathology, 19(7), 1563-1579. http://doi:10.1111/mpp.12644. Hancinsk, R., Mih, D., Mrkvov, M., Candresse, T. y Glasa, M. (2020). Plant viruses infecting Solanaceae family members in the cultivated and wild environments: A Review. Plants, 9, 667. https://doi:10.3390/plants9050667 Heredia, S. C. (2016). Erradicación de Onion Yellow Dwarf Virus (OYDV) en cebolla Shallot (Allium cepa var. Aggregatum), mediante el cultivo de meristemas, quimioterapia y termoterapia para la producción de bulbos libres de virus [Tesis de pregrado, Universidad San Francisco de Quito]. Repositorio Digital USFQ. http://repositorio.usfq.edu.ec/handle/23000/5198 Hill, J. H. (2001). First Report of Transmission of Soybean mosaic virus and Alfalfa mosaic virus by Aphis glycines in the New World. Plant Disease, 85(5). https://doi.org/10.1094/PDIS.2001.85.5.561C. Horváth, J. (1970). Reaction of Physalis species to plant viruses. The Cape gooseberry as a symptomless carrier of Potato virus X and Y. Acta Phytopathologica, 5, 65-72- Horváth, J. (1996). Ornamental physalis species as perennial virus hosts. Acta Horticulturae, 432, 204-211. https://doi:10.17660/actahortic.1996.432.25 Jaramillo, H. (2017). Análisis del transcriptoma y viroma de Passiflora edulis f. edulis en cultivos de Antioquia utilizando métodos de secuenciación de nueva generación [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/62808 Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2018). Molecular characterization of Soybean mosaic virus (SMV) infecting Purple passion fruit (Passiflora edulis f. edulis) in Antioquia, Colombia. Archives of Phytopathology and Plant Protection, 1–20. https://doi:10.1080/03235408.2018.1505411 Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2019). Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passion fruit (Passiflora edulis f. edulis). Revista Facultad Nacional de Agronomia Medellín, 72(1), 8643-8654. https://doi.org/10.15446/rfnam.v72n1.69438 Jover-Gil, S., Beeri, A., Fresnillo, P., Samach, A. y Candela, H. (2018). Complete genome sequence of a novel virus, classifiable within the Potyviridae family, which infects passion fruit (Passiflora edulis). Archives of Virology. https://doi:10.1007/s00705-018-3983-7 Joy, P. P. y Sherin, C.G., (2012). DISEASES OF PASSION FRUIT (Passiflora edulis): Pathogen, Symptoms, Infection, Spread & Management. https://www.researchgate.net/publication/306017818_DISEASES_OF_PASSION_FRUIT_Passiflora_edulis_Pathogen_Symptoms_Infection_Spread_Management Kreuze, J. Vaira, A. M. Mezel, W. Candresse, T. Zavriev, S. K. Hammond, J. y Ryu, H. (2020). ICTV virus taxonomy profile: Alphaflexiviridae. Journal of General Virology. https://doi.org/10.1099/jgv.0.001436 Langmead, B. y Salzberg, S. L. (2012). Fast gapped-read alignment with Bowtie 2. Nature Methods, 9(4), 357–359. https://doi:10.1038/nmeth.1923 Lee, J. S., Cho, W.K., Choi, H.-S. y Kim, K.-H. (2011). RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses. The Plant Pathology Journal, 27(3), 291-296. https://doi.org/10.5423/PPJ.2011.27.3.291 Legge, A. (1974). Notes on the history, cultivation and uses of Physalis peruviana L. Journal of the Royal Horticultural Society, 99 (7), 310−314. Li, R. y Hartung, J. S. (2007). Reverse transcription-polymerase chain reaction-based detection of plant viruses. Current protocols in microbiology. https://doi.org/10.1002/9780471729259.mc16c01s6 Li, H. y Durbin, R. (2010). Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics (Oxford, England), 26(5), 589-595. https://doi.org/10.1093/bioinformatics/btp698 Li, H., Zhou, P., Yang, Q., Shen, Y., Deng, J., Li, L. y Zhao, D. (2011). Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis “edulis” and Passiflora edulis “flavicarpa.” Journal of Ethnopharmacology, 133(3), 1085-1090. https://doi:10.1016/j.jep.2010.11.039 Li, H., Liu, X., Michaud, J.P., Zhi, H., Li, K., Li, Z. y Li, X. (2018). Host Plant Infection by Soybean mosaic virus Reduces the Fitness of Its Vector, Aphis Glycines (Hemiptera: Aphididae). Journal of Economic Entomology, 111(5). https://doi.org/10.1093/JEE/TOY165. Liberato, J. y Murilo, Z. (s.f.) Diseases of Passionfruit (Passiflora spp.). https://www.apsnet.org/edcenter/resources/commonnames/Pages/Passionfruit.aspx Mabele, A. S., Were, H. K., Owuor Ndong’a, M. F. y Mukoye, B. (2021). Occurrence and genetic diversity of groundnut rosette assistor virus in western Kenya. Crop Protection, 139, 105381. https://doi.org/https://doi.org/10.1016/j.cropro.2020.105381 Maree, H. J., Fox, A., Al Rwahnih, M., Boonham, N. y Candresse, T. (2018). Application of HTS for Routine Plant Virus Diagnostics: State of the Art and Challenges. Frontiers in Plant Science, 9. https://doi:10.3389/fpls.2018.01082 Marín, M. Gutiérrez, P.A. (2016). Principios de virología molecular de plantas tropicales. Agrosavia. http://editorial.agrosavia.co/index.php/publicaciones/catalog/book/69 Martínez, M.L. (1998). Revisión de Physalis Sección Epeteiorhiza (Solanaceae). Universidad Nacional Autónoma de México, 69, 71-117. http://www.revistas.unam.mx/index.php/bot/article/view/1909 Medford, J. (1992). Vegetative Apical Meristems. Plant Cell, 4, 1029-1039. https://doi.org/10.1105/tpc.4.9.1029 Menzel, M. Y. (1951). The Cytotaxonomy and Genetics of Physalis. Proceedings of the American Philosophical Society, 95(2), 132–183. http://www.jstor.org/stable/31433310434.2002.00740. Ministerio de Agricultura y Desarollo Rural [Minagricultura]. (2020). Cadena de pasifloras. https://sioc.minagricultura.gov.co/Pasifloras/Documentos/2020-06-30%20Cifras%20Sectoriales.pdf Mink, G. I. (1992). Ilarvirus Vectors BT - Advances in Disease Vector Research (K. F. Harris (ed.); pp. (261–281). Springer New York. https://doi.org/10.1007/978-1-4612-2910-0_8 Miranda, D., G. Fischer, C. Carranza, S. Magnitskiy, F. Casierra-Posada, W. Piedrahíta y Flórez, L. (2009). Cultivo, poscosecha y comercialización de las pasifloráceas en Colombia: maracuyá, granadilla, gulupa y curuba. Sociedad Colombiana de Ciencias Hortícolas, Bogotá.http://www.asohofrucol.com.co/archivos/biblioteca/biblioteca_118_cultivo_poscosechavp.pdf Mituti, T., Spadotti, D. M. A., Narita, N. y Rezende, J. A. M. (2018). First Report of Sida Mottle Alagoas Virus Infecting Passiflora edulis in Brazil. Plant Disease, 103(1), 169. https://doi.org/10.1094/PDIS-06-18-1068-PDN Morales, F.J., Lozano, I., Castaño, M., Arroyave, J., Velasco, A.C. y Varon, F. (2002). Partial characterization of a tymovirus infecting passion fruit in Colombia, South America. Journal of Phytopathology 150(4-5), 292-296. https://doi:10.1046/j.1439-Morton, J. (1987). Passionfruit. Fruits of warm climates,320–328 Mortimer, S. M., Jones, M. G. K., Jones, R. A. C., Thomson, G. y Dwyer, G. I. (2009). A single tube, quantitative real-time RT-PCR assay that detects four potato viruses simultaneously. Journal of Virological Methods, 161(2), 289-296. https://doi:10.1016/j.jviromet.2009.06.027 Naidu, R.A. y Hughes, J.D.A. (2001). Methods for the detection of plant virus diseases [conferencia]. Proceedings of a conference on Plant Virology in Sub Saharan Africa, Ibadan, Nigeria, (pp. 233-253). https://hdl.handle.net/10568/96508 Nakasato, K., Fujioka, S., Sugawara, Y., Ono, T., Nishio, T. y Tsuda, S. (2020). First detection of two potyviruses, uraria mosaic virus and passiflora mosaic virus Y, from passionfruit in Japan. Journal of General Plant Pathology. https://doi:10.1007/s10327-020-00932-4 Nascimento, A., Santana, E. y Braz, A. (2006). Cowpea aphid-borne mosaic virus (CABMV) is widespread in passionfruit in Brazil and causes passionfruit woodiness disease. Archives of Virology 151, 1797–1809.https://doi.org/10.1007/s00705-006-0755-6 Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. y Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic acids research, 28(12), E63. https://doi.org/10.1093/nar/28.12.e63 Ocampo, J. y Wyckhuys, K. (Eds.) (2012.). Tecnología para el cultivo de la gulupa (Passiflora edulis f. edulis Sims) en Colombia. Centro de Bio-Sistemas de la Universidad Jorge Tadeo Lozano, Centro Internacional de Agricultura Tropical - CIAT y Ministerio de Agricultura y Desarrollo Rural, República de Colombia. https://www.researchgate.net/publication/259946957_Tecnologia_para_el_cultivo_de_la_Gulupa_en_Colombia_Passiflora_edulis_f_edulis_Sims_Purple_Passion_Fruit Olivares-Tenorio, M.-L., Dekker, M., Verkerk, R. y Boekel, M. A. J. S. (2016). Health-promoting compounds in cape gooseberry (Physalis peruviana L.): Review from a supply chain perspective. Trends in Food Science & Technology, 57, 83–92. https://doi:10.1016/j.tifs.2016.09.009 Panno, S., Matić, S., Tiberini, A., Caruso, A. G., Bella, P., Torta, L., Stassi, R. y Davino, A. S. (2020). Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology. Plants (Basel, Switzerland), 9(4), 461. https://doi.org/10.3390/plants9040461 Parrella, G. y Sorrentino, D. (2009). Identification of a Cucumber mosaic virus Isolate from Passiflora edulis in Southern Italy and Validation of Subgroup Identification by In Silico Restriction Fragment Length Polymorphism. Journal of Phytopathology, 157(11–12), 762–767. https://doi.org/10.1111/j.1439-0434.2009.01604.x Pássaro, C. (Ed). (2014). Physalis peruviana L: fruta andina para el mundo. http://www.cyted.org/es/noticias/libro-physalis-peruviana-l-fruta-andina-para-el-mundo Pavlovic, S., Klaassen, K., Stankovic, B., Stojiljkovic, M. y Zukic, B. (2020). Next-Generation Sequencing: The Enabler and the Way Ahead. Microbiomics, 175–200. https://doi:10.1016/b978-0-12-816664-2.00009-8 Perea, M., Rodríguez, N.C., Fisher, G., Velázquez, M., y Mican, Y. (2010). Solanaceae, Biotecnología aplicada al mejoramiento de los cultivos de frutas tropicales. Universidad Nacional de Colombia, 466-490. https://www.uneditorial.com/biotecnologia-aplicada-al-mejoramiento-de-los-cultivos-de-frutas-tropicales-agropecuario.html Perry, K. L., Zhang, L. y Palukaitis, P. (1998). Amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the Aphids Myzus persicae and Aphis gossypii. Virology, 242(1), 204–210. https://doi.org/10.1006/viro.1998.8991 Prakash, O., Misra, A.K., Singh, S.J. y Srivastava, K.M. (1988). Isolation, purification and electron microscopy of mosaic virus of cape gooseberry. International Journal of Tropical Plant Diseases. 6(1), 85-87. Prammanee, S., Thumjamras, S., Chiemsombat, P. y Pipattanawong, N. (2011). Efficient shoot regeneration from direct apical meristem tissue to produce virus-free purple passion fruit plants. Crop Protection, 30(11), 1425-1429. https://doi:10.1016/j.cropro.2011.07.008 PROCOLOMBIA. (s.f.). Uchuva (goldenberry). https://docs.procolombia.co/int-procolombia/es/exportaciones/ficha_uchuva_final.pdf PROCOLOMBIA. (2021). Aumentan los pedidos de frutas colombianas en Europa. https://procolombia.co/noticias/aumentan-los-pedidos-de-frutas-colombianas-en-europa Puente, L. A., Pinto-Muñoz, C. A., Castro, E. S. y Cortés, M. (2011). Physalis peruviana Linnaeus, the multiple properties of a highly functional fruit: A review. Food Research International, 44(7), 1733-1740. https://doi.org/https://doi.org/10.1016/j.foodres.2010.09.034 Reuter, J. A., Spacek, D. V. y Snyder, M. P. (2015). High-Throughput Sequencing Technologies. Molecular Cell, 58(4), 586-597. https://doi:10.1016/j.molcel.2015.05.004 Salamon, P. y Palkovics, L. (2005). Occurrence of colombian datura virus in Brugmansia hybrids, Physalis peruviana L. and Solanum muricatum Ait. in Hungary. Acta Virologica. 49, 117-122 Sepúlveda, M., Cardona, D., Gallo, Y., Higuita, M., Gutiérrez, P. y Marín, M. (2022). Virome analysis for identification of viruses associated with asymptomatic infection of purple passion fruit (Passiflora edulis f. edulis) in Colombia, The Journal of Horticultural Science and Biotechnology, 1–14. https://doi: 10.1080/14620316.2021.1973583 Vaca-Vaca, J.C., Carrasco-Lozano, E.C. y López-López, K. (2017). Molecular identification of a new begomovirus infecting yellow passion fruit (Passiflora edulis) in Colombia. Archives of Virology 162, 573–576. https://doi.org/10.1007/s00705-016-3098-y Agindotan, B.O., P.J. Shiel, P.H. Berger. 2007. Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan real-time RT-PCR. Journal of Virological Methods 142(1-2): 1-9. https://doi: 10.1016/j.jviromet.2006.12.012. Agronet, 2020. Red de información y comunicación del sector Agropecuario Colombiano. https://www.agronet.gov.co/estadistica/Paginas/home.aspx (accessed 20 september 2021). Álvarez, N., H. Jaramillo, Y. Gallo, P. Gutiérrez, M. Marín. 2018. Molecular characterization of Potato virus Y (PVY) and Potato virus V (PVV) isolates naturally infecting Cape gooseberry (Physalis peruviana) in Antioquia, Colombia. Agronomía Colombiana 36(1): 13–23. https://dx.doi.org/10.15446/agron.colomb.v36n1.65051 Ayala, M., P. González, P. Gutiérrez, J. Cotes, M. Marín. 2010. Caracterización serológica y molecular de potyvirus asociados a la virosis del tomate de árbol en Antioquia (Colombia). Acta Biológica Colombiana 15: 143-162. Brewer, E., M. Cao, B. Gutierrez, M. Bateman, R. Li. 2020. Discovery and molecular characterization of a novel trichovirus infecting sweet cherry. Virus Genes 56: 380-385. https://doi.org/10.1007/s11262-020-01743-7 Bushmanova, E., D. Antipov, A. Lapidus, A.D. Prjibelski. 2019. rnaSPAdes: a de novo transcriptome assembler and its application to RNA-Seq data, GigaScience 8(9): 1-13. https://doi.org/10.1093/gigascience/giz100 Duarte, P.D.S.G., S.B.F. Galvino-Costa, S.R.R. de Paula Ribeiro, A.R. Figueira. 2012. Complete genome sequence of the first Andean strain of potato virus S from Brazil and evidence of recombination between PVS strains. Archives of Virology 157: 1357–1364. https://doi.org/10.1007/s00705-012-1289-8 Duque, M., M. Marín, P.A. Gutiérrez. 2017. Genome comparison and primer design for detection of Tamarillo leaf malformation virus (TaLMV). Archives of Phytopathology and Plant Protection 50: 713-726. https://doi.org/10.1080/03235408.2017.1370934 Edgar, R.C., R.M. Drive, M. Valley. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340. Fischer, G., L.M. Melgarejo. 2020. The ecophysiology of cape gooseberry (Physalis peruviana L.) - an Andean fruit crop. A review. Revista Colombiana de Ciencias Hortícolas 14(1): 76-89. https://doi.org/10.17584/rcch.2020v14i1.10893 Fuchs, M., C. Schmitt-Keichinger, H. Sanfaçon. 2017. A renaissance in Nepovirus research provides new insights into their molecular interface with hosts and vectors. Advances in Virus Research 97: 61-105. https://doi.org/10.1016/bs.aivir.2016.08.009 Gallo, Y. 2020. Caracterización molecular del viroma de plantas solanáceas de importancia económica en Antioquia. PhD. thesis Biotechnology. Universidad Nacional de Colombia sede Medellín. García, N., P. Gutiérrez, M. Marín. 2013. Detección y cuantificación del Potato mop-top virus (PMTV) en Colombia mediante qRT-PCR. Acta Agronómica 62(2): 120-128. Gil, J.F., J.M. Cotes, E.P. González, M. Marín. 2011. Caracterización genotípica de aislamientos colombianos del potato mop-top virus (PMTV, Pomovirus). Actualidades Biológicas 33(94): 69–84. Gil, J.F., I. Adams, N. Boonham, S.L. Nielsen, M. Nicolaisen. 2016. Molecular and biological characterization of Potato mop-top virus (PMTV, Pomovirus) isolates from the potato-growing regions of Colombia. Plant Pathology 65: 1210–1220. https://doi.org/10.1111/ppa.12491. Gish, W., D.J. States. 1993. Identification of protein coding regions by database similarity search. Nature Genetics 3: 266–272. https://doi.org/10.1038/ng0393-266 Gutiérrez, P.A., J.F. Alzate, M.M. Montoya. 2015a. Complete genome sequence of an isolate of Potato virus X (PVX) infecting cape gooseberry (Physalis peruviana) in Colombia. Virus Genes 50(3): 518-522. https://10.1007/s11262-015-1181-1 Gutiérrez, P.A., J.F. Alzate, M. Marín. 2015b. Genome sequence of a virus isolate from tamarillo (Solanum betaceum) in Colombia: evidence for a new potyvirus. Archives of Virology 160(2): 557-560. https://doi.org/10.1007/s00705-014-2296-8 Gutiérrez, P., A. Rivillas, D. Tejada, S. Giraldo, A. Restrepo, M. Ospina, S. Cadavid, Y. Gallo, M. Marín. 2021. PVDP: A portable open source pipeline for detection of plant viruses in RNAseq data. A case study on potato viruses in Antioquia (Colombia). Physiological and Molecular Plant Pathology 113: 101604. https://doi.org/10.1016/j.pmpp.2021.101604 Hull, R. 2013. Plant Virology. 5th Edition. Academic Press, London. 918 p. Johansen, E., M.C. Edwards, R.O. Hampton. 1994. Seed transmission of viruses: Current Perspectives. Annual Review of Phytopathology 32: 363-386. Jones, D.T., W.R. Taylor, J.M. Thornton. 1992. The rapid generation of mutation data matrices from protein sequences. Computer Applications in the Biosciences 8: 275-282. https://doi.org/10.1093/bioinformatics/8.3.275 Kim, S., M. Park, S.I. Yeom, Y.M. Kim, J.M. Lee, H.A. Lee, E. Seo, J. Choi, K. Cheong, K.T. Kim, K. Jung, G.W. Lee, S.K. Oh, C. Bae, S.B. Kim, H.Y. Lee, S.Y. Kim, M.S. Kim, B.C. Kang, Y.D. Jo, H.B. Yang, H.J. Jeong, W.H. Kang, J.K. Kwon, C. Shin, J.Y. Lim, J.H. Park, J.H. Huh, J.S. Kim, B.D. Kim, O. Cohen, I. Paran, M.C. Suh, S.B. Lee, Y.K. Kim, Y. Shin, S.J. Noh, J. Park, Y.S. Seo, S.Y. Kwon, H.A. Kim, J.M. Park, H.J. Kim, S.B. Choi, P.W. Bosland, G. Reeves, S.H. Jo, B.W. Lee, H.T. Cho, H.S. Choi, M.S. Lee, Y. Yu, Y. Do Choi, B.S. Park, A. van Deynze, H. Ashrafi, T. Hill, W.T. Kim, H.S. Pai, H.K. Ahn, I. Yeam, J.J. Giovannoni, J.K. Rose, I. Sørensen, S.J. Lee, R.W. Kim, I.Y. Choi, B.S. Choi, J.S. Lim, Y.H. Lee, D. Choi. 2014. Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species. Nature Genetics 2014 46(3): 270-278. https://doi.org/10.1038/ng.2877 King, A.M.Q. 2012. Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego. Kitajima, E.W. 2020. An annotated list of plant viruses and viroids described in Brazil (1926-2018). Biota Neotropica 20(2): e20190932. https://doi.org/10.1590/1676-0611-BN-2019-0932. Kumar, S., G. Stecher, M. Li, C. Knyaz, K. Tamura. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547-1549. https://doi.org/10.1093/molbev/msy096. Martelli, G.P., T. Candresse, S. Namba. 1994. Trichovirus, a new genus of plant viruses. Archives of Virology 134(3-4): 451-455. https://doi.org/10.1007/BF01310583. Mistry, J., S. Chuguransky, L. Williams, M. Qureshi, G.A. Salazar, E.L.L. Sonnhammer, S.C.E. Tosatto, L. Paladin, S. Raj, L.J. Richardson, R.D. Finn, A. Bateman. 2021. Pfam: The protein families database in 2021. Nucleic Acids Research, 49(D1): D412-D419. https://doi.org/10.1093/nar/gkaa913 Posada, D., K.A. Crandall. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817-818. https://doi.org/10.1093/bioinformatics/14.9.817 Procolombia. 2020. Uchuva (Goldenberry). https://docs.procolombia.co/int-procolombia/es/exportaciones/ficha_uchuva_final.pdf (accessed 10 October 2021) Saitou, N., M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4(4): 406–425. https://10.1093/oxfordjournals.molbev.a040454 Savenkov, E.I., M.Y. Sandgren, J.P.T. Valkonen. 1999. Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus. Journal of General Virology 80: 2779-2784. https://10.1099/0022-1317-80-10-2779 Singh, M., Singh, R.P., Fageria, M.S., Nie, X., Coffin, R., Hawkins, G. 2013. Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers. American Journal of Potato Research, 90: 43–50. https://doi.org/10.1007/s12230-012-9274-z Tamura K. 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Molecular Biology and Evolution 9(4): 678-687. https://10.1093/oxfordjournals.molbev.a040752 Vallejo, D., Gutiérrez, P., M. Marín. 2016. Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk. Agronomía Colombiana 34: 51–60. https://doi.org/10.15446/agron.colomb.v34n1.53161. Agronet (2021). Área, producción y rendimiento de Gulupa en Colombia. In: https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1; accessed: October 2020. Bhat AI, Hohn T, Selvarajan R (2016). Badnaviruses: The current global scenario. Viruses 8(6): 177. doi: 10.3390/v8060177 Bushmanova E, Antipov D, Lapidus A, Prjibelski AD (2019) rnaSPAdes: a de novo transcriptome assembler and its application to RNA-Seq data. Gigascience 8(9): giz100. doi: 10.1093/gigascience/giz100 Cardona D, Higuita M, Posada J, Gallo Y, Marín M, Gutiérrez P (2022a). Viruses infecting purple passion fruit (Passiflora edulis f. edulis) in Southwestern Antioquia (Colombia). Archives of Phytopathology and Plant Protection. Archives of Phytopathology and Plant Protection, 55(12): 1394–1409. doi: 10.1080/03235408.2022.2098588 Cardona, D., Restrepo, A., Higuita, M., Gallo, Y., Marin, M., y Gutiérrez, P. (2022b). Natural infection of purple passion fruit (Passiflora edulis f. edulis) by a novel member of the family Tymoviridae in Colombia. Acta virologica, 66(3):254–262. doi:10.4149/av_2022_310 Cardona D, Gallo Y, Higuita M, Hoyos R, Gutiérrez P, Marín M (2022c). Detección molecular de virus en cultivos, plántulas y semillas de gulupa (Passiflora edulis f. edulis) en el oriente de Antioquia. Bioagro: 34(2). Chabannes M, Gabriel M, Aksa A, Galzi S, Dufayard JF, Iskra-Caruana ML, Muller E (2020). Badnaviruses and banana genomes: a long association sheds light on Musa phylogeny and origin. Molecular Plant Pathology 22:216-230. doi: 10.1111/mpp.13019 Chomczynski P, Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162(1): 156-159. doi: 10.1006/abio.1987.9999 Cuspoca, J (2007). Evaluación de virus de tomate de árbol (Solanum betaceum) en plantas indicadoras y su detección por PCR. Thesis in Agronomy. Faculty of Agronomy. Universidad Nacional de Colombia. Bogotá. 31 p. Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus, 12: 13-15. Feng JL, Chen SN, Tang XS, Ding XF, Du ZY, Chen JS (2006). Quantitative determination of cucumber mosaic virus genome RNAs in virions by Real-Time Reverse Transcription-Polymerase Chain Reaction. Acta Biochimica et Biophysica Sinica, 38(10): 669-676. doi: 10.1111/j.1745-7270.2006.00216.x Gao L, Ding X, Li K, Liao W, Zhong Y, Ren R, Liu Z, Adhimoolam K, Zhi H (2015). Characterization of Soybean mosaic virus resistance derived from inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars. Theoretical and Applied Genetics, 128: 1489-1505. doi: 10.1007/s00122-015-2522-0 Gillaspie AG Jr, Pio-Ribeiro G, Andrade GP, Pappu HR (2001). RT-PCR Detection of seedborne Cowpea aphid-borne mosaic virus in peanut. Plant Disease, 85(11): 1181-1182. doi: 10.1094/PDIS.2001.85.11.1181 Gonçalves Z, de Jesus O, Cerqueira-Silva CB; Diniz RP, Soares TL, de Oliveira EJ (2017). Methodological approaches to assess passion fruit resistance (Passiflora spp.) to passionfruit woodiness disease. Bioscience Journal, 33(6): 1441-1451. doi: 10.14393/BJ-v33n6a2017-36619 Gordillo LA (2011). Incidencia del Soybean mosaic virus en cultivos de Gulupa (Passiflora edulis Sims) en Cundinamarca y estudio de su diversidad en Colombia. Master’s Thesis in Microbiology. Faculty of Sciences. Universidad Nacional de Colombia. Bogotá. 106 p. https://repositorio.unal.edu.co/handle/unal/9885 Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS (2018). UFBoot2: Improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution 35(2): 518-522. doi: 10.1093/molbev/msx281. PMID: 29077904 Jaramillo H, Marín M and Gutiérrez P (2018). Molecular characterization of Soybean mosaic virus (SMV) infecting Purple passion fruit (Passiflora edulis f. edulis) in Antioquia, Colombia. Archives of Phytopathology and Plant Protection, 51(11-12): 617-636. doi: 10.1080/03235408.2018.1505411 Jaramillo H, Marín M and Gutiérrez P (2019). Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passion fruit (Passiflora edulis f. edulis). Revista Facultad Nacional de Agronomía Medellín, 72(1): 8643-8654. doi: 10.15446/rfnam.v72n1.69438 Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS (2017). ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14(6): 587-589. doi: 10.1038/nmeth.4285 Katoh K, Standley DM (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4): 772-780. doi: 10.1093/molbev/mst010 King AMQ (2012). Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego. King AMQ (2012). Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego. Melgarejo, L, Fischer, G, Cuca Suárez, L, Hernández Gómez, M, Hoyos Carvajal, L, Magnitskiy, S, Brochero, H, Miranda Lasprilla, D, Álvarez-Flórez, F, Ávila Murillo, M, Delgado Ávila, W, Mendoza Forero, C, Lizarazo Hernández, K, Solarte Cruz, M, Hurtado Clopatosky, S, Plazas Rodríguez, E, García Morantes, J, Ramírez Soler, C, Márquez-Niño, F, Moreno Echeverry, D, Sandoval, J, Flechas Bejarano, N, Díaz Ardila, H, Cárdenas Pira, W, Torres Moya, E, Cruz Ospina, S, Toro Tobón, G, Paz Figueroa, V y Rodríguez Castillo, N. (2019). Gulupa (Passiflora edulis), curuba (Passiflora tripartita), aguacate (Persea americana) y tomate de árbol (Solanum betaceum) Innovaciones. Universidad Nacional de Colombia, Bogotá. https://repositorio.unal.edu.co/handle/unal/79864 Munguti F, Maina S, Nyaboga EN, Kilalo D, Kimani E, Macharia M, Holton T (2019). Transcriptome sequencing reveals a complete genome sequence of cowpea aphid-borne mosaic virus from passion fruit in Kenya. Microbiology Resource Announcements, 8(2): e01607-18. doi: 10.1128/MRA.01607-18. Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ (2015). IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32(1): 268-274. doi: 10.1093/molbev/msu300 Robinson JT, Thorvaldsdóttir H, Winckler W, Guttman M, Lander ES, Getz G, Mesirov JP (2011). Integrative Genomics Viewer. Nature Biotechnology 29(1): 24-26. doi: 10.1038/nbt.1754 Xanthis CK, Maliogka VI, Lecoq H, Dezbiez C, Tsvetkov I, Katis NI (2015). First report of cucumber mosaic virus infecting watermelon in Greece and Bulgaria. Journal of Plant Pathology 97(2): 391-403. doi: 10.4454/JPP.V97I2.00
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv	Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.uri.spa.fl_str_mv	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.accessrights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	Atribución-NoComercial-SinDerivadas 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.extent.spa.fl_str_mv	xii, 222 páginas
dc.format.mimetype.spa.fl_str_mv	application/pdf
dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Medellín - Ciencias - Maestría en Ciencias - Biotecnología
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
institution	Universidad Nacional de Colombia
bitstream.url.fl_str_mv	https://repositorio.unal.edu.co/bitstream/unal/83420/3/license.txt https://repositorio.unal.edu.co/bitstream/unal/83420/10/Bacca%20David%20Michelle.licencia.cap.1.pdf https://repositorio.unal.edu.co/bitstream/unal/83420/11/Bacca%20David%20Michelle.licencia.cap.2.pdf https://repositorio.unal.edu.co/bitstream/unal/83420/12/Bacca%20David%20Michelle.licencia.cap.3.pdf https://repositorio.unal.edu.co/bitstream/unal/83420/13/Bacca%20David%20Michelle.licencia.cap.4.pdf https://repositorio.unal.edu.co/bitstream/unal/83420/14/Bacca%20David%20Michelle.licencia.cap.5.pdf https://repositorio.unal.edu.co/bitstream/unal/83420/4/1037654739.2022.pdf https://repositorio.unal.edu.co/bitstream/unal/83420/15/1037654739.2022.pdf.jpg
bitstream.checksum.fl_str_mv	eb34b1cf90b7e1103fc9dfd26be24b4a c35d6e5beb2a767ae474f81fe798a13e c9035d234e07cb41695ae39f2571781c cf3c64bfc0801fe7794a600bebaa8495 9fd00672bff9949b5b7888a8194cf317 f2f8bad1d19cbc2eaf191840a1f9e13a 5c4479b71731f63429b9a674dae6a144 dd4dcd7a90e0512116642cdda0d3aa89
bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5 MD5 MD5 MD5 MD5 MD5
repository.name.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv	repositorio_nal@unal.edu.co
_version_	1814089291440062464
spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Marín Montoya, Mauricio Alejandroea05ef2dc8f58ea502fc56bf288d0ab9600Gutiérrez Sánchez, Pablo Andrés03fa1cb1dfa744c01ad629ca741ddd94600Bacca David, Michelle0780ffaa143d32044f6d699a10039767Higuita Valencia, MonicaGallo Garcia, YulianaRestrepo Escobar, AndreaPosada Rua,JaimeGiraldo Ramirez, SusanaMartinez Mira, AnnyBacca David, MichelleRestrepo, AndreaMarín, MauricioGutiérrez, PabloBiotecnología vegetalBacca, Michelle [0000-0002-8277-9593]2023-02-10T18:33:49Z2023-02-10T18:33:49Z2022https://repositorio.unal.edu.co/handle/unal/83420Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/En los últimos años en Colombia, los cultivos de gulupa (Passiflora edulis f. edulis Sims) y uchuva (Physalis peruviana L.) han tomado gran relevancia en el sector agrícola de la región andina. Esto se debe a que sus frutos presentan características organolépticas y nutricionales muy apetecidas en el mercado internacional y nacional. El aumento del área sembrada de estos frutales ha estado acompañado del incremento de diversos problemas fitosanitarios, entre los que se destacan aquellos de origen viral. Sin embargo, el conocimiento de los agentes causales, vectores y efectos sobre los rendimientos es aún incipiente para estas enfermedades. Esta información es fundamental para proponer programas de manejo integrado que resulten en aumentos en la productividad y longevidad de los cultivos, así como en la calidad de los frutos. Con el fin de contribuir al aumento del conocimiento sobre el viroma de estos cultivos, en este proyecto de investigación se planteó la utilización de metodologías moleculares de última generación para determinar los virus más prevalentes en cultivos y material de siembra de gulupa y uchuva del Suroeste de Antioquia; adicionalmente, se realizó una evaluación inicial de posibles vectores y reservorios biológicos (arvenses) para dichos virus. Los resultados obtenidos indicaron que PMTV fue el virus más prevalente en los cultivos de uchuva, ya que se detectó en el 100% de muestras sintomáticas, 80% de muestras asintomáticas y en el 60% de las muestras de semillas. Los análisis HTS revelaron la presencia de una nueva especie de virus estrechamente relacionada con el género Trichovirus, denominada tentativamente como Physalis chlorosis virus (PhyCV). También se obtuvo la secuencia del genoma de nuevos aislamientos de TaLMV, PhyVNV y PVY que afectan a P. peruviana. Por otra parte, mediante PCR y confirmación morfológica, fue posible identificar en el cultivo de uchuva ocho especies de plantas arvenses: Commelina diffusa, Ageratum conyzoides, Erigeron sumatrensis, Galinsoga quadriradiata, Bidens pilosa, Sonchus oleraceus, Persicaria nepalensis y Plantago australis; así como dos especies de insectos posiblemente vectores de virus: Trialeurodes vaporariorum y Frankliniella occidentalis. Utilizando la prueba de RT-qPCR con cebadores específicos, se detectó la ocurrencia de los virus PVS, PVX, TaLMV y PMTV en algunas arvenses asociadas al cultivo de uchuva. En el culltivo de gulupa, se detectaron los virus CMV, PFYMV, PpLDV y GBVA tanto en muestras de campo como en material de siembra; los virus CABMV y SMV no fueron encontrados en ninguna de las muestras analizadas. Para el caso de arvenses, en los cultivos de gulupa del Suroeste se identificaron nueve especies: Persicaria nepalensis, Commelina diffusa, Cardamine flexuosa, Galinsoga quadriradiata, Bidens pilosa, Ageratum conyzoides, Erigeron sumatrensis, Sonchus oleraceus e Ipomoea purpurea, así como seis posibles insectos vectores de virus: Lachnopus sp., Aphis fabae, Tetranychus sp., Pseudococcus sp., Brachycaudus helichrysi y Neohydatothrips burungae. También se detectó al PFYMV y GBVA en algunas de las arvenses y el CMV en el insecto A. fabae. Finalmente, se realizó una prueba inicial de la técnica aislamiento/cultivo de meristemos in vitro en uchuva para la eliminación viral, obteniéndo vitroplántulas libres de los virus PVY, PVS, PVX y CGIV-1. En gulupa se empleó quimioterapia ex vitro en plántulas bajo diferentes concentraciones de ribavirina (225, 250 y 275 ppm) en un tiempo de inmersión radicular de 2 h y 30 min; sin embargo, con este tratamiento no se logró obtener plántulas libres de PFYMV, pero sí con menor titulo viral, determinado en términos de valores de Ct en pruebas de RT-qPCR. Estos resultados señalan la importancia de implementar conjuntamente métodos de secuenciación masiva y técnicas moleculares como herramientas eficientes para el diagnóstico de infecciones virales que apoyen los programas de manejo integrado de enfermedades virales, vigilancia curentenaria y obtención de material certificado en estos frutales andinos. (Texto tomado de la fuente)In recent years, purple passion fruit (Passiflora edulis f. edulis Sims) and cape gooseberry (Physalis peruviana L.) have become some of the most important crops in the Andean region of Colombia. Due to their unique organoleptic characteristics, both fruits have an increasingly high demand in local and international markets. However, the rapid expansion of these crops has resulted in a significant increase of several phytopathological problems, particularly viral diseases. Unfortunately, little is known about the viruses, their associated vectors and/or their effect on yields in both crops. This type of information is key for the implementation of integrated disease management programs aimed at improving the productivity and longevity of crops, as well as the quality of fruits. To increase our current knowledge on the virology of these crops, this work intended to determine which viruses are the most prevalent in commercial fields and planting material of purple passion fruit and cape gooseberry in southwestern Antioquia using state of the art molecular techniques. In addition, a preliminary investigation on potential vectors and plant weed reservoirs to these viruses was also performed. In cape gooseberry, results indicate that PMTV is the most prevalent virus as it was detected in 100% of symptomatic plants, 80% of asymptomatic plants, and 60% of seed samples. High-throughput sequencing revealed a new viral species related to the genus Trichovirus tentatively named Physalis chlorosis virus (PhyCV). Genome information on TaLMV, PhyVNV and PVY circulating in the region was also obtained. With respect to potential alternate hosts and vectors, a combination of standard PCR with morphological analyses revealed eight weeds associated with P. peruviana: Commelina diffusa, Ageratum conyzoides, Erigeron sumatrensis, Galinsoga quadriradiata, Bidens pilosa, Sonchus oleraceus, Persicaria nepalensis and Plantago australis; and two potential insect vectors: Trialeurodes vaporariorum and Frankliniella occidentalis. On the other hand, RT-qPCR analysis detected PVS, PVX, TaLMV and PMTV is some of the associated weeds. With respect to purple passion fruit, the viruses CMV, PFYMV, PpLDV and GBVA were detected in field samples and planting material; however, no evidence from the presence of CABMV nor SMV was found in any of the tested samples. Nine weed plants associated to purple passion fruit crops were identified in southwestern Antioquia: Persicaria nepalensis, Commelina diffusa, Cardamine flexuosa, Galinsoga quadriradiata, Bidens pilosa, Ageratum conyzoides, Erigeron sumatrensis, Sonchus oleraceus and Ipomoea purpurea. Six potential insect vectors were also identified in this crop: Lachnopus sp., Aphis fabae, Tetranychus sp., Pseudococcus sp., Brachycaudus helichrysi and Neohydatothrips burungae. RT-qPCR detected PFYMV and GBVA in some weeds, and CMV in the insect A. fabae. Finally, a preliminary test of virus clean-up methods using in vitro meristem culture in cape gooseberry resulted in the generation of vitroplants negative for PVY, PVS, PVX and CGIV-1. In purple passion fruit, evaluation of ribavirin chemotherapy (225, 250 y 275 ppm) of ex vitro plantlets at one immersion time (2 h and 30 min) failed to eliminate the virus PFYMV; however, results suggest that this method does reduce the viral load for this virus as evidenced by RT-qPCR. Together, these results highlight the importance of using HTS in combination with molecular techniques for reliable testing of viruses and to support disease management programs, quarantine vigilance and the generation of certified planting material of these important fruit crops.Universidad Nacional de Colombia sede Medellín, Universidad CES y Caribbean Exotics.MaestríaMagíster en Ciencias - BiotecnologíaVirología vegetalÁrea curricular Biotecnologíaxii, 222 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesUchuva (Physalis peruviana) - Enfermedades y plagasVirus fitopatógenosCultivo in vitroRT-qPCRSecuenciación masivaPassiflora edulis f. edulisPhysalis peruvianavirus fitopatógenosCultivo in vitroUchuva (Physalis peruviana)Gulupa (Passiflora edulis f. edulis)High-throughput sequencingplant virusesIn vitro plant tissueCaracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de AntioquiaMolecular characterization of viruses in crops of cape gooseberry (Physalis peruviana) and purple passion fruit (Passiflora edulis f. edulis) in southwestern AntioquiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMLaReferenciaInternational Committee on Taxonomy of Viruses [ICTV]. (2022). Bromoviridae- Positive Sense RNA Viruses. https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/bromoviridaeInternational Committee on Taxonomy of Viruses [ICTV]. (2022). Potexvirus - Alphaflexiviridae - Positive-sense RNA Viruses.https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/alphaflexiviridae/1330/genus-potexvirusInternational Committee on Taxonomy of Viruses [ICTV]. (2022). Potyvirus - Potyviridae - Positive-sense RNA Viruses. https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/potyviridae/572/genus-potyvirusRhoads, A. y Au, K. F. (2015). PacBio Sequencing and Its Applications. Genomics, Proteomics & Bioinformatics, 13(5), 278–289. https://doi:10.1016/j.gpb.2015.08.002Riascos, D. (2011). Caracterización etiológica de la Roña de la gulupa (Passiflora edulis Sims.) en la región del Sumapaz (Cundinamarca). [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/9360Rodríguez, M., Niño, N., Cutler, J., Langer, J., Casierra-Posada, F., Miranda, D., Bandte, M. y Büttner, C. (2016). Certificación de material vegetal sano en Colombia: un análisis crítico de oportunidades y retos para controlar enfermedades ocasionadas por virus. Revista Colombiana De Ciencias Hortícolas, 10(1), 164-175. https://doi.org/10.17584/rcch.2016v10i1.4921Roingeard, P., Raynal, P. I., Eymieux, S. y Blanchard, E. (2019). Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety. Reviews in medical virology, 29(1). https://doi.org/10.1002/rmv.2019Ryu, K. H. y Hong, J. S. (2008). Potexvirus. Encyclopedia of Virology, 310-313. https://doi:10.1016/b978-012374410-4.00738-xSanger, F., Nicklen, S. y Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America, 74(12), 5463-5467. https://doi.org/10.1073/pnas.74.12.5463Santillan, F. W., Fribourg, C. E., Adams, I. P., Gibbs, A. J., Boonham, N., Kehoe, M. A., Maina, S. y Jones, R. A. C. (2018). The Biology and Phylogenetics of Potato virus S Isolates from the Andean Region of South America. Plant Disease, 102(5), 869–885. https://doi.org/10.1094/PDIS-09-17-1414-RESerrato, A., Flores, L., Aportela, J. y Sierra, E. (2014). PCR: reacción en cadena de la polimerasa. En: Herramientas moleculares aplicadas en ecología (pp. 53-73). http://www2.inecc.gob.mx/publicaciones2/libros/710/pcr.pdfShaffer, L. (2019). Inner Workings: Portable DNA sequencer helps farmers stymie devastating viruses. Proceedings of the National Academy of Sciences, 116(9), 3351-3353. https://doi.org/10.1073/pnas.1901806116Spadotti, D., Bello, V., Favara, G., Stangarlin, O., Krause-Sakate, R. y Rezende, J. (2019). Passiflora edulis: new natural host of Melochia yellow mosaic virus in Brazil. Australasian Plant Disease Notes, 14. https://doi.org/10.1007/s13314-019-0354-5Taïwe, G. S. y Kuete, V. (2017). Passiflora edulis. Medicinal Spices and Vegetables from Africa, 513–526. http://doi:10.1016/b978-0-12-809286-6.00024-8Tamayo, A. R. L., Betancur, C. E. y García, V. E. L. (2014). Manual técnico del cultivo de uchuva bajo buenas prácticas agrícolas.Torrance, L. (1998). Developments in serological methods to detect and identify plant viruses. Plant Cell, Tissue and Organ Culture, 52, 27-32. https://doi.org/10.1023/A:1005943823182Torre, C. (2019). Detección y caracterización de virus epidemiológicamente relevantes en cultivos de tomate y cucurbitáceas [Tesis de doctorado, Universidad de Murcia]. http://hdl.handle.net/10201/74370Trenado, H.P., Fortes, I.M. y Louro, D. (2007). Physalis ixocarpa and P. peruviana, new natural hosts of Tomato chlorosis virus. European Journal of Plant Pathology, 118, 193-196. https://doi.org/10.1007/s10658-007-9129-5Vallejo, D. C., Gutiérrez, P. y Marín, M. (2016). Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja. Agronomía Colombiana, 34(1), 51-60. https://doi.org/10.15446/agron.colomb.v34n1.53161.Vidal, A.H., Sanches M.M., Alves-Freitas, D.M.T., Abreu, E.F.M., Lacorte, C., Pinheiro-Lima, B., Rosa, R.C.C., Jesus, O.N., Campos, M.A., Varsani, A. y Ribeiro S.G (2018). First World Report of Cucurbit aphid-borne yellows virus infecting Passionfruit. Plant Disease, 102(12): 2665. https://doi: 10.1094/PDIS-04-18-0694-PDNVillamor, D. E. V., Ho, T., Al Rwahnih, M., Martin, R. R. y Tzanetakis, I. (2019). High Throughput Sequencing in Plant Virus Detection and Discovery. Phytopathology. https://doi:10.1094/phyto-07-18-0257-rvwVunsh, R., Rosner, A. y Stein, A. (1990). The use of the polymerase chain reaction (PCR) for the detection of bean yellow mosaic virus in gladiolus. Annals of Applied Biology, 117. https://doi.org/10.1111/j.1744-7348.1990.tb04822.xWang, Q., Liu, Y., Xie, Y. y You, M. (2006). Cryotherapy of Potato Shoot Tips for Efficient Elimination of Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY). Potato Research, 49(2), 119-129. https://doi:10.1007/s11540-006-9011-4Wang, Q., Cuellar, W., Rajamäki, M., Hirata, Y. y Valkonen, J. (2008).Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology, 9(2), 237-250. https://doi: 10.1111/j.1364-3703.2007.00456.xWang, Q., Panis, B., Engelmann, F., Lambardi, M. y Valkonen J. (2009).Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation.Trends Plant Science, 14(3), 351-363. https://doi: 10.1016/j.tplants.2008.11.010.Wang, Y., Yang, Q. y Wang, Z. (2015). The evolution of nanopore sequencing. Frontiers in Genetics, 5, 449. https://doi.org/10.3389/fgene.2014.00449Wang, M. R., Cui, Z. H., Li, J. W., Hao, X. Y., Zhao, L. y Wang, Q. C. (2018). In vitro thermotherapy-based methods for plant virus eradication. Plant Methods 14(1), 87. https://doi.org/10.1186/s13007-018-0355-yWardrop, E. A., Gray, A. B., Singh, R. P. y Peterson, J. F. (1989). Aphid transmission of potato virus S. American Potato Journal, 66(8), 449-459. https://doi.org/10.1007/BF02855437Waswa, M., Kakuhenzire, R. y Ochwo-Ssemakula, M. (2017). Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks. African Journal of Plant Science, 11(3), 61-70. https://doi.org/10.5897/AJPS2016.1497Wylie, S. J. y Jones, M. G. K. (2010). The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses. Archives of Virology, 156(3), 479-482. https://doi:10.1007/s00705-010-0845-3Zapata, J., Londoño, M., Diaz, Cipriano, A. y Saldarriaga, A. (2002). Manejo del cultivo de la uchuva en Colombia. AGROSAVIA. https://repository.agrosavia.co/handle/20.500.12324/12833?locale-attribute=esZhang, S., Zhao, T., Liu, J., Zi, L., Li, X., Wang, Y., Zhang, Z., Li, D., Yu, J y Han, C. (2019). First Report of Cucurbit Aphid-Borne Yellows Virus in Passion fruit plants exhibiting mosaic and mottling in China. Plant Disease. https://doi:10.1094/pdis-07-19-1378-pdnZibadi, S. y Watson, R. R. (2004). Passion Fruit (Passiflora edulis). Evidence-Based Integrative Medicine, 1(3), 183-187. https://doi:10.2165/01197065-200401030-00005Al-Shahwan, I. M., Abdalla, O. A., Al-Saleh, M. A. y Amer, M. A. (2017). Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi J. Biol. Sci. 24(6), 1336-1343. https://doi.org/ 10.1016/j.sjbs.2016.02.022.Anwar, I., Bukhari, H. A., Nahid, N., Rashid, K., Amin, I., Shaheen, S., Hussain, K. y Mansoor, S. (2020). Association of cotton leaf curl Multan betasatellite and Ageratum conyzoides symptomless alphasatellite with tomato leaf curl New Delhi virus in Luffa cylindrica in Pakistan. Australasian Plant Pathology, 49(1), 25–29. https://doi.org/10.1007/s13313-019-00668-6Cámara de comercio de Bogotá. (2015). Manual Gulupa. http://hdl.handle.net/11520/14314Chen, G., Pan, H., Xie, W., Wang, S., Wu, Q., Fang, Y., Shi, X. y Zhang, Y. (2013). Virus infection of a weed increases vector attraction to and vector fitness on the weed. Scientific Reports, 3(1), 2253. https://doi.org/10.1038/srep02253Cheng, T., Xu, C., Lei, L., Li, C., Zhang, Y. y Zhou, S. (2016), Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity. Molecular Ecology Resources, 16, 138-149. https://doi.org/10.1111/1755-0998.12438Cutler, J., Langer, J., Von, S., Acosta, O., Casierra, F., Castañeda, A., Betancourt, M., Cuellar, W., Arvydas, E., Altenbach, D. y Büttner, C. (2018). Preliminary evaluation of associated viruses in production systems of cape gooseberry, purple passion fruit, and rose. Revista Colombiana de Ciencias Hortícolas, 12(2), 390-396. https://doi:10.17584/rcch.2018vl2i2.7799Cuspoca, J. (2007). Evaluación de virus de tomate de árbol (Solanum betaceum) en plantas indicadoras y su detección por PCR. Tesis en agronomia. Facultad de agronomia. Universidad Nacional de Colombia. Bogotá. 31 p.DANE. (2020). Exportaciones-Histórico. https://www.dane.gov.co/index.php/estadisticas-por-tema/comercio-internacional/exportaciones/exportaciones-historicosDoyle, J. (1991). DNA Protocols for Plants. Molecular Techniques in Taxonomy. Springer. https://doi:10.1007/978-3-642-83962-7_18Duque, M., Marín, M. y Gutiérrez, P.A. (2017). Genome comparison and primer design for detection of Tamarillo leaf malformation virus (TaLMV). Archives of Phytopathology and Plant Protection, 50, 713-726. https://doi.org/10.1080/03235408.2017.1370934EPPO. (2021). Galinsoga quadriradiata. https://gd.eppo.int/taxon/GASCI/pestsFeng, J.L., Chen, S.N., Tang, X.S., Ding, X.F., Du, Z.Y. y Chen, J.S. (2006). Quantitative determination of cucumber mosaic virus genome RNAs in virions by Real-Time Reverse Transcription-Polymerase Chain Reaction. Acta Biochimica et Biophysica Sinica, 38(10): 669-676. https://doi.org/10.1111/j.1745-7270.2006.00216.xGao, L. X., Ding, K., Li, W., Liao, Y., Zhong, R., Ren, Z., Liu, K., Adhimoolam, K. y H. Zhi. (2015). Characterization of Soybean mosaic virus resistance derived from inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars. Theoretical and Applied Genetics 128, 1489-1505. https://doi.org/10.1007/s00122-015-2522-0Hebert, P. D., Cywinska, A., Ball, S. L. y deWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings. Biological sciences, 270(1512), 313–321. https://doi.org/10.1098/rspb.2002.2218Hebert, P. D. N., Penton, E. H., Burns, J. M., Janzen, D. H. y Hallwachs, W. (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences, 101(41), 14812–14817. doi:10.1073/pnas.0406166101Hobbs, H. A., Eastburn, D. M., D’Arcy, C. J., Kindhart, J. D., Masiunas, J. B., Voegtlin, D. J., Weinzierl, R. A. y McCoppin, N. K. (2000). Solanaceous Weeds as Possible Sources of Cucumber mosaic virus in Southern Illinois for Aphid Transmission to Pepper. Plant Disease, 84(11), 1221–1224. https://doi.org/10.1094/PDIS.2000.84.11.1221Huang, C. J., Liu, Y., Yu, H. Q., Liu, B. Z., y Qing, L. (2016). Bidens pilosa is a Natural Host of Tomato spotted wilt virus in Yunnan Province, China. Plant Disease, 100(9), 1957. https://doi.org/10.1094/PDIS-03-16-0344-PDNHull, R. (2013). Plant Virology. Fifth Edition. Academic Press.Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2018). Molecular characterization of Soybean mosaic virus (SMV) infecting Purple passion fruit (Passiflora edulis f. edulis) in Antioquia, Colombia. Archives of Phytopathology and Plant Protection, 1–20. https://doi:10.1080/03235408.2018.1505411Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2019). Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passion fruit (Passiflora edulis f. edulis). Revista Facultad Nacional de Agronomia Medellín, 72(1), 8643-8654. https://doi.org/10.15446/rfnam.v72n1.69438Junco, M.C., Silva, C.D.C., do Carmo, C.M., Kotsubo, R.Y., de Novaes, T.G. y Molina, R.D.O. (2020). Identification of potential hosts plants of Cowpea aphid-borne mosaic virus. Journal of Phytopathology. 169, 45– 51. https://doi.org/10.1111/jph.12957Kamaal, N., Akram, M., Gupta, S. y Agnihotri, A. (2014). Ageratum conyzoides Harbours Mungbean yellow mosaic India virus. Plant Pathology Journal, 13. https://doi.org/10.3923/ppj.2014.59.64Kaliciak, A. y Syller, J. (2009). New hosts of Potato virus Y (PVY) among common wild plants in Europe. European Journal of Plant Pathology124, 707–713 https://doi.org/10.1007/s10658-009-9452-0Kashina, B., MABAGALA, B. y Mpunami, A. (2003). First Report of Ageratum conyzoides L. and Sida acuta Burm F. as New Weed Hosts of Tomato Yellow Leaf Curl. Plant Protection Science, 39. https://doi.org/10.17221/3822-PPSKumar, S., Stecher, G., Li, M., Knyaz, C. y Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096Marín, M. y Gutiérrez, P.A. (2016). Principios de virología molecular de plantas tropicales. Agrosavia. http://editorial.agrosavia.co/index.php/publicaciones/catalog/book/69Miranda, D., G. Fischer, C. Carranza, S. Magnitskiy, F. Casierra-Posada, W. Piedrahíta y Flórez, L. (2009). Cultivo, poscosecha y comercialización de las pasifloráceas en Colombia: maracuyá, granadilla, gulupa y curuba. Sociedad Colombiana de Ciencias Hortícolas, Bogotá.http://www.asohofrucol.com.co/archivos/biblioteca/biblioteca_118_cultivo_poscosechavp.pdfMiranda, D., Carranza Gutiérrez, C. E., Fischer Gebauer, G., Jerez, C., Ramírez Godoy, A., Forero de la Rotta, M. C., Quevedo, K., Lanchero, O., Obregón Corredor, D., Chaves Córdoba, B., Pedraza Flautero, M., Plaza Trujillo, G. A., Martínez Maldonado, F. E. y Torres Arango, C. (2016). Problemas de campo asociados al cultivo de uchuva (Physalis peruviana L.) (Primera edición). Universidad Nacional de Colombia. Facultad de Ciencias Agrarias. http://ezproxy.unal.edu.co/login?url=https://search.ebscohost.com/login.aspx?direct=true&db=cat02704a&AN=unc.000836877&lang=es&site=eds-livePlaza, G. A. y Pedraza, M. (2007). Reconocimiento y caracterización ecológica de la flora arvense asociada al cultivo de uchuva. Agronomía Colombiana, 25, 306–313.Pandey, N., Tiwari, A., Rao, G. y Shukla, K. (2011). Detection and identification of Ageratum enation virus infecting Ageratum conyzoides in India. Acta Phytopathologica et Entomologica Hungarica, 46(2), 203–211. https://doi.org/10.1556/aphyt.46.2011.2.4Rojas, J. (2020). Bidens pilosa (blackjack). Invasive Species Compendium. CABI. https://doi:10.1079/ISC.9148.20203483381Roossinck, M.J., Saha, P., Wiley, G.B., Quan, J., White, J.D., Lai, H. et al. (2010) Ecogenomics: using massively parallel pyrosequencing to understand virus ecology. Molecular Ecology, 19, 81–88. https://doi.org/10.1111/j.1365-294X.2009.04470.xSalazar, L. F., Muller, G., Querci, M., Zapata, J. L. y Owens, R. A. (2000). Potato yellow vein virus: its host range, distribution in South America and identification as a crinivirus transmitted by Trialeurodes vaporariorum. Annals of Applied Biology, 137(1), 7–19. https://doi.org/https://doi.org/10.1111/j.1744-7348.2000.tb00052.xSavenkov, E.I., Sandgren, M.Y. y Valkonen, J.P.T. (1999). Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus. Journal of General Virology, 80, 2779-2784. https://10.1099/0022-1317-80-10-2779Schena, L., Nigro, F., Ippolito, A. y Gallitelli, D. (2004). Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. European Journal of Plant Pathology, 110(9), 893-908. https://doi.org/10.1007/s10658-004-4842-9Shibuya, Y., Sakata, J., Sukamto, N., Kon, T., Sharma, P. y Ikegami, M. (2007). First Report of Pepper yellow leaf curl Indonesia virus in Ageratum conyzoides in Indonesia. Plant Disease, 91(9), 1198. https://doi.org/10.1094/PDIS-91-9-1198BSingh, M., Singh, R.P., Fageria, M.S., Nie, X., Coffin, R. y Hawkins, G. (2013). Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers. American Journal of Potato Research, 90, 43–50. https://doi.org/10.1007/s12230-012-9274-zSingh, G., Singh, N. P. y Singh, R. (2014). Food plants of a major agricultural pest Aphis gossypii Glover (Homoptera: Aphididae) from India: An updated checklist. International Journal of Research Studies in Biosciences, 3, 1-26.Tan, H. N. P. y Wong, S. M. (1993). Some Properties of Singapore Ageratum Yellow Vein Virus (SAYVV). Journal of Phytopathology, 139(2), 165–176. https://doi.org/https://doi.org/10.1111/j.1439-0434.1993.tb01412.xWamonje, F. O., Tungadi, T. D., Murphy, A. M., Pate, A. E., Woodcock, C., Caulfield, J. C., Mutuku, J. M., Cunniffe, N. J., Bruce, T. J. A., Gilligan, C. A., Pickett, J. A. y Carr, J. P. (2020). Three Aphid-Transmitted Viruses Encourage Vector Migration From Infected Common Bean (Phaseolus vulgaris) Plants Through a Combination of Volatile and Surface Cues. Frontiers in Plant Science, 11. https://www.frontiersin.org/article/10.3389/fpls.2020.613772Xanthis, C.K., Maliogka, V.I., Lecoq, H., Dezbiez, C., Tsvetkov, I. y Katis, N.I. (2015). First report of cucumber mosaic virus infecting watermelon in Greece and Bulgaria, Journal of Plant Pathology, 97(2), 391-403. http://dx.doi.org/10.4454/JPP.V97I2.007Yazdkhasti, E., Hopkins, R. y Kvarnheden, A. (2021). Reservoirs of Plant Virus Disease: Occurrence of Wheat Dwarf Virus and Barley/Cereal Yellow Dwarf Viruses in Sweden. Plant Pathol. 70, 1552–1561. https://doi.org/10.1111/ppa.13414Yu, J., Xue, J.-H. y Zhou, S.-L. (2011). New universal matK primers for DNA barcoding angiosperms. Journal of Systematics and Evolution, 49, 176-181. https://doi.org/10.1111/j.1759-6831.2011.00134.xZapata, J., Londoño, M., Diaz, Cipriano, A. y Saldarriaga, A. (2002). Manejo del cultivo de la uchuva en Colombia. AGROSAVIA. https://repository.agrosavia.co/handle/20.500.12324/12833?locale-attribute=esAGUILERA P., TACHIQUIN M., ROCHA M.G., PINEDA B. 2014. PCR en tiempo real. Herramientas moleculares aplicadas en ecología: aspectos teóricos y prácticos. 175–201.GARCÍA N., GUTIÉRREZ P.A, MARÍN M.M. 2013. Detección y cuantificación del Potato mop-top virus (PMTV) en Colombia mediante qRT-PCR. Acta Agronómica.62(2):120-128.NIE X & SINGH R.P. 2001. A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves and tubers. Journal of Virological Methods.91:37–49. https://10.1016/s0166-0934(00)00242-1.PÁSSARO, C. (Ed). 2014. Physalis peruviana L: fruta andina para el mundo. http://www.cyted.org/es/noticias/libro-physalis-peruviana-l-fruta-andina-para-el-mundoSAMBROOK J., & RUSSELL, D. 2001. Molecular Cloning: A Laboratory Manual (3.a ed.). Spring Harbor Laboratory Press.SCHENA L., NIGRO F., IPPOLITO A., GALLITELLI D. 2004. Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. European Journal of Plant Pathology.110(9):893-908. https://doi.org/10.1007/s10658-004-4842-9SHIRIMA R. R., MAEDA D. G., KANJU E., CEASAR G., TIBAZARWA F. I., LEGG J. P. 2017. Absolute quantification of cassava brown streak virus mRNA by real-time qPCR. Journal of Virological Methods.245:5-13. https://doi:10.1016/j.jviromet.2017.03.003WONG M. L. Y MEDRANO J. F. 2005. Real-time PCR for mRNA quantitation. BioTechniques.39(1):75-85. https://doi:10.2144/05391rv01Andrade, S., Fosneca, L., Silva, M., Faleiro, F. y Junqueira, N. (2010). Estudos Preliminares para o Uso de Termoterapia ex vitro em Maracujazeiro-Azedo visando à Eliminação de Vírus-do- endurecimento-dos-frutos. Boletim de Pesquisa e Desenvolvimento, 267Balamuralikrishnan, M., Doraisamy, S., Ganapathy, T. y Viswanathan, R. (2002). Combined effect of chemotherapy and meristem culture on sugarcane mosaic virus elimination in sugarcane. Sugar Tech, 4(1-2), 19–25. https://doi:10.1007/bf02956875Cutler, J., Langer, J., Von, S., Acosta, O., Casierra, F., Castañeda, A., Betancourt, M., Cuellar, W., Arvydas, E., Altenbach, D. y Büttner, C. (2018). Preliminary evaluation of associated viruses in production systems of cape gooseberry, purple passion fruit, and rose. Revista Colombiana de Ciencias Hortícolas, 12(2), 390-396. https://doi:10.17584/rcch.2018vl2i2.7799Duque, M., Marín, M. y Gutiérrez, P.A. (2017). Genome comparison and primer design for detection of Tamarillo leaf malformation virus (TaLMV). Archives of Phytopathology and Plant Protection, 50, 713-726. https://doi.org/10.1080/03235408.2017.1370934Faccioli G. (2001). Control de virus de la papa mediante cultivos de meristemo y corte de tallo, termoterapia y quimioterapia. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0842-6_28Gil, J. F., Adams, I., Boonham, N., Nielsen, S. L. y Nicolaisen, M. (2016). Molecular and biological characterization of Potato mop-top virus (PMTV, Pomovirus) isolates from the potato-growing regions of Colombia. Plant Pathology, 65(7), 1210–1220. https://doi.org/10.1111/ppa.12491Gómez, J.E., F. Morales, J. Arroyave. (1997). Mosaic disease of Physalis peruviana in Colombia. ASCOLFI Informa. 23:52.Gong, H., Igiraneza, C. y Dusengemungu, L. (2019). Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review. American Journal of Potato Research. 96, 379-389. https://doi.org/10.1007/s12230-019-09720-zGrout, B. W. W. (1999). Meristem-Tip Culture for Propagation and Virus Elimination. Plant Cell Culture Protocols, 115-126. https://doi.org/10.1385/1-59259-583-9:115Hansen, A. J. y Lane, W. D. (1985). Elimination of apple chlorotic leafspot virus from apple shoot cultures by ribavirin. Plant Disease, 69(2), 134-135.Heredia, S. C. (2016). Erradicación de Onion Yellow Dwarf Virus (OYDV) en cebolla Shallot (Allium cepa var. Aggregatum), mediante el cultivo de meristemas, quimioterapia y termoterapia para la producción de bulbos libres de virus [Tesis de pregrado, Universidad San Francisco de Quito]. Repositorio Digital USFQ. http://repositorio.usfq.edu.ec/handle/23000/5198Mellor, F. C. y Stace, R. (1987). Virus-Free Potatoes Through Meristem Culture. Potato, 30-39. https://doi:10.1007/978-3-642-72773-3_3Montiel , O., Pastelín, M., Ventura, E., Castañeda, O., Gonzalez, M. y Guevara, M. (2011). Tropical and subtropical agroecosystem, 13(3), 537-542.Nasir, I., y Husnain, T. (2010). Strategies to control Potato Virus Y under in vitro conditions. Pakistan Journal Phytopathology, 22, 63–70.Pennazio, S. y Appiano, A. (1975). Potato virus X aggregates in ultrathin sections of Datura meristematic dome. Phytopathologia Mediterranea, 14(1), 12-15. http://www.jstor.org/stable/42684242Savenkov, E.I., Sandgren, M.Y. y Valkonen, J.P.T. (1999). Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus. Journal of General Virology, 80, 2779-2784. https://10.1099/0022-1317-80-10-2779Simpkins, I., Walkey, D. G. A. y Neely, H. A. (1981). Chemical suppression of virus in cultured plant tissues. Annals of Applied Biology, 99(2), 161-169. https://doi.org/10.1111/j.1744-7348.1981.tb05143.xVallejo, D., Gutiérrez, P. y M, Marín. (2016). Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk. Agronomía Colombiana 34, 51–60. https://doi.org/10.15446/agron.colomb.v34n1.53161.Singh, B. (2015). Effect of antiviral chemicals on in vitro regeneration response and production of PLRV-free plants of potato. Journal of Crop Science and Biotechnology, 18(5), 341-348. https://doi.org/10.1007/s12892-015-0069-xWang, Q., Panis B, Engelmann, F., Lambardi, M. y Valkonen, J. (2009). Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation.Trends Plant Science, 14(3), 351-363. https://doi.org/10.1111/j.1744-7348.2008.00308.xWang, M. R., Cui, Z. H., Li, J. W., Hao, X. Y., Zhao, L. y Wang, Q. C. (2018). In vitro thermotherapy-based methods for plant virus eradication. Plant Methods 14(1), 87. https://doi.org/10.1186/s13007-018-0355-yZhang, Z., Wang, Q.C., Spetz, C. y Blystad, D.R. (2019). In vitro therapies for virus elimination of potato-valuable germplasm in Norway. Scientia Horticulturae, 249, 7-14. https://doi.org/10.1016/j.scienta.2019.01.027Fuchs, M., C. Schmitt-Keichinger, H. Sanfaçon. 2017. A renaissance in Nepovirus research provides new insights into their molecular interface with hosts and vectors. Advances in Virus Research 97(61-105). https://doi.org/10.1016/bs.aivir.2016.08.009Fischer, Gerhard., Almanza-Merchán., Pedro José. y Miranda, Diego. (2014). Importancia y cultivo de la uchuva (Physalis peruviana L.). Revista Brasileira de Fruticultura, 36(1), 01-15. https://dx.doi.org/10.1590/0100-2945-441/13Gong, H., Igiraneza, C. y Dusengemungu, L. (2019). Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review. American Journal of Potato Research. 96, 379-389. https://doi.org/10.1007/s12230-019-09720-zHobbs, H. A., Eastburn, D. M., D’Arcy, C. J., Kindhart, J. D., Masiunas, J. B., Voegtlin, D. J., Weinzierl, R. A. y McCoppin, N. K. (2000). Solanaceous Weeds as Possible Sources of Cucumber mosaic virus in Southern Illinois for Aphid Transmission to Pepper. Plant Disease, 84(11), 1221–1224. https://doi.org/10.1094/PDIS.2000.84.11.1221Johansen, E., M.C. Edwards, R.O. Hampton. 1994. Seed transmission of viruses: CurrentKing AMQ (2012). Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego.Pallas, V., F. Aparicio, M.C. Herranz, K. Amari, M.A. Sanchez-Pina, A. Myrta, J.A. Sanchez-Navarro. 2012. Ilarviruses of Prunus spp.: a continued concern for fruit trees. Phytopathology 102(12), 1108-20. https://10.1094/PHYTO-02-12-0023-RVWPlaza, G. A. y Pedraza, M. (2007). Reconocimiento y caracterización ecológica de la flora arvense asociada al cultivo de uchuva. Agronomía Colombiana, 25, 306–313.Mellor, F. C. y Stace, R. (1987). Virus-Free Potatoes Through Meristem Culture. Potato, 30-39. https://doi:10.1007/978-3-642-72773-3_3Wamonje, F. O., Tungadi, T. D., Murphy, A. M., Pate, A. E., Woodcock, C., Caulfield, J. C., Mutuku, J. M., Cunniffe, N. J., Bruce, T. J. A., Gilligan, C. A., Pickett, J. A. y Carr, J. P. (2020). Three Aphid-Transmitted Viruses Encourage Vector Migration From Infected Common Bean (Phaseolus vulgaris) Plants Through a Combination of Volatile and Surface Cues. Frontiers in Plant Science, 11. https://www.frontiersin.org/article/10.3389/fpls.2020.613772Agindotan, B. O., Shiel, P. J., y Berger, P. H. (2007). Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan® real-time RT-PCR. Journal of Virological Methods, 142(1-2), 1-9. http://doi:10.1016/j.jviromet.2006.12.012Agronet. (2018). Reporte: Área, Producción y Rendimiento Nacional por Cultivo. https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1Almanza, P.J. (2000). Propagación. En, Flórez, V.J., Fischer, G. y Sora, A.D (Eds.). Producción, poscosecha y exportación de la uchuva (Physalis peruviana L.) (pp. 27–40). Universidad Nacional de Colombia, Bogotá. https://repository.agrosavia.co/handle/20.500.12324/30703AlMaarri, K., Massa, R. y AlBiski, F. (2012). Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants. Plant Biotechnology, 29(3), 237-243. http://doi:10.5511/plantbiotechnology.12.0215aÁlvarez, N., Jaramillo, H., Gallo, Y., Gutiérrez, P. y Marín, M. (2018). Molecular characterization of Potato virus Y (PVY) and Potato virus V (PVV) isolates naturally infecting cape gooseberry (Physalis peruviana) in Antioquia, Colombia. Agronomía Colombiana, 36(1), 13-23. https://dx.doi.org/10.15446/agron.colomb.v36n1.65051Analdex. (2020). Exportación de Gulupa en 2020. https://www.analdex.org/2021/02/25/exportacion-de-gulupa-en-2020/Analdex. (2021). Informe exportaciones de uchuva. https://www.analdex.org/2021/10/20/informe-exportaciones-de-uchuva/Analdex. (s.f.). ¡Uchuva / Goldenberry nuestra Embajadora de frutas Colombianas para el mundo!. https://www.analdex.org/2020/06/30/uchuva-goldenberry-nuestra-embajadora-de-frutas-colombianas-para-el-mundo/Andrade, S., Fosneca, L., Silva, M., Faleiro, F. y Junqueira, N. (2010). Estudos Preliminares para o Uso de Termoterapia ex vitro em Maracujazeiro-Azedo visando à Eliminação de Vírus-do- endurecimento-dos-frutos. Boletim de Pesquisa e Desenvolvimento, 267.Ateş, S.Y., İnan, S., Ayyaz, M., Dündar, I. y Yabangülü, D. (2019). Effect of thermotherapy in combination with meristem culture for eliminating potato virus Y (PVY) and potato virus S (PVS) from infected seed stocks. The Journal of Animal & Plant Sciences, 29(2), 549-555. http://www.thejaps.org.pk/docs/V-29-02/26.pdfBang, B., Lee, J., Kim, S., Park, J., Nguyen, T. T. y Seo, Y. S. (2014). A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus. The Plant Pathology Journal, 30(3), 310-315. https://doi.org/10.5423/PPJ.NT.03.2014.0025Bartholomaus, A., A. de la Rosa, J.O. Santos, L.E. Acero y W, Moosbrugger. (1990). Physalis peruviana. El manto de la tierra - Flora de los Andes. Guía de 150 especies de la flora andina. (pp. 163-164). Lerner, Bogotá.Benscher, D., Pappu, S.S., Niblett, C.L., Varón de Agudelo, F., Morales, F., Hodson, E., Álvarez, E., Acosta, O. y Lee, R.F. (1996). A strain of Soybean mosaic virus infecting Passiflora spp. In Colombia. Plant Disease. 80, 258-262. http://doi: 10.1094/PD-80-0258.Bhat, A.I. y Rao, G.P. (2020). Rolling Circle Amplification (RCA). En, Characterization of Plant Viruses (pp. 377-381). Springer Protocols Handbooks.https://doi.org/10.1007/978-1-0716-0334-5_2Blanco, J. (2000). Manejo de enfermedades. En, V. J., Flóres, G., Fischer, Á. D., Sora, (Eds). Cultivos de uchuva. Producción, pos-cosecha y exportación de la uchuva (Physalis peruviana L.) (pp. 57-65). Universidad Nacional de Colombia.Blawid, R., Silva, J. M. F. y Nagata, T. (2017). Discovering and sequencing new plant viral genomes by next-generation sequencing: description of a practical pipeline. Annals of Applied Biology, 170(3), 301–314. http://doi:10.1111/aab.12345Bolger, A. M., Lohse, M. y Usadel, B. (2014). Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics (Oxford, England), 30(15), 2114-2120. https://doi.org/10.1093/bioinformatics/btu170Boonham, N., Laurenson, L., Weekes, R. y Mumford, R. (2008). Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR. Methods in Molecular Biology, 249-258. http://doi:10.1007/978-1-59745-062-1_19Boonham, N., Kreuze, J., Winter, S., van der Vlugt, R., Bergervoet, J., Tomlinson, J. y Mumford, R. (2014). Methods in virus diagnostics: From ELISA to next generation sequencing. Virus Research, 186, 20-31. http://doi:10.1016/j.virusres.2013.12.007Boratyn, G. M., Thierry-Mieg, J., Thierry-Mieg, D., Busby, B. y Madden, T. L. (2019). Magic-BLAST, an accurate RNA-seq aligner for long and short reads. BMC Bioinformatics, 20(1). http:// doi:10.1186/s12859-019-2996-xCamelo, V.M. (2010). Detección e identificación de los virus patógenos de cultivos de gulupa (Passiflora edulis Sims.) en la región de Sumapaz (Cundinamarca). [Tesis de Maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/6860Cámara de comercio de Bogotá. Manual Gulupa. (2015). https://bibliotecadigital.ccb.org.co/bitstream/handle/11520/14314/Gulupa.pdf?sequence=1&isAllowed=yChen, S., Yu, N., Yang, S. Zhong, B.y Lan, H. (2018). Identification of Telosma mosaic virus infection in Passiflora edulis and its impact on phytochemical contents Virology Jurnal 15, 168. https://doi.org/10.1186/s12985-018-1084-6Chiemsombat, P., Prammanee, S. y Pipattanawong, N. (2014). Occurrence of Telosma mosaic virus causing passion fruit severe mosaic disease in Thailand and immunostrip test for rapid virus detection. Crop Protection, 63, 41–47. https://doi.org/https://doi.org/10.1016/j.cropro.2014.04.023Chong, YH., Cheng, YH., Cheng, HW. (2018). The virus causing passionfruit woodiness disease in Taiwan is reclassified as East Asian passiflora virus. Journal of General Plant Pathology 84, 208-220. https://doi.org/10.1007/s10327-018-0777-4Clark, M.F. y A.N. Adams. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34(3), 475-483. https://doi.org/10.1099/0022-1317-34-3-475Corrales-Cabra, E., Higuita, M., Hoyos, R., Gallo, Y., Marín, M. y Gutiérrez, P. (2021). Prevalence of RNA viruses in seeds, plantlets, and adult plants of cape gooseberry (Physalis peruviana) in Antioquia (Colombia). Physiological and Molecular Plant Pathology 116, 101715. https://doi.org/10.1016/j.pmpp.2021.101715.Corrales-Cabra, E. (2022). Caracterización genómica de los virus que infectan los cultivos de uchuva (Physalis peruviana) en Antioquia para el apoyo de los programas de certificación de semilla [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/82181Crestani, O.A., Kitajima, E.W., Lin, M.T. y Marinho V.L. (1986). Passion Fruit Yellow Mosaic Virus, a New Tymovirus Found in Brazil. The American Phytopathological Society 76(9), 951-955. https://doi:10.1094 / Phyto-76-951Cutler, J., Langer, J., Von, S., Acosta, O., Casierra, F., Castañeda, A., Betancourt, M., Cuellar, W., Arvydas, E., Altenbach, D. y Büttner, C. (2018). Preliminary evaluation of associated viruses in production systems of cape gooseberry, purple passion fruit, and rose. Revista Colombiana de Ciencias Hortícolas, 12(2), 390-396. https://doi:10.17584/rcch.2018vl2i2.7799DANE. (2020). Exportaciones-Histórico. https://www.dane.gov.co/index.php/estadisticas-por-tema/comercio-internacional/exportaciones/exportaciones-historicosD'Arcy, W. (1991). The Solanaceae since 1976, with a review of its biogeography. En: Hawkes, J., Lester, R.L., Nee, M. y Estrada N (Eds.), Solanaceae: Taxonomy, chemistry, evolution, 75-137.Davis, J. A., Radcliffe, E. B. y Ragsdale, D. W. (2005). Soybean aphid, Aphis glycines Matsumura, a new vector of Potato virus Y in potato. American Journal of Potato Research, 82(3), 197-201. https://doi:10.1007/bf02853585.Daza, P.A. y Rodríguez, P.A. (2006). Enfermedades de origen viral en plantas de uchuva (Physalis peruviana L.) en el Departamento de Cundinamarca. [Tesis de pregrado, Pontificia Universidad Javeriana] Bogotá.Díaz, A., Quiñones, M., Arana, F., Soto, M. y Hernández, Annia. (2010). Potyvirus: características generales, situación de su diagnóstico y determinación de su presencia en el cultivo del pimiento en Cuba. Revista de Protección Vegetal, 25(2), 69-79. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522010000200001&lng=es&tlng=.Domier, L. L., Hobbs, H. A., McCoppin, N. K., Bowen, C. R., Steinlage, T. A., Chang, S., Wang, Y. y Hartman, G. L. (2011). Multiple loci condition seed transmission of soybean mosaic virus (SMV) and SMV-induced seed coat mottling in soybean. Phytopathology, 101(6), 750–756. https://doi.org/10.1094/PHYTO-09-10-0239.Eiras, M., Costa, I.F.D., Chaves, A.L.R., Colariccio, A., Harakava, R., Tanaka, F.A.O., Garcêz, R.M. y Silva, L.A. (2012). First report of a tospovirus in a commercial crop of Cape gooseberry in Brazil. New Disease Reports 25, 25. http://dx.doi.org/10.5197/j.2044-0588.2012.025.025Esquivel, A. F., Rezende, J. A. M., Lima, E. F. B., Kitajima, E. W. y Diniz, F. O. (2018). First Report of Groundnut ring spot virus on Physalis peruviana in Brazil. Plant Disease, 102(7), 1468–1468. http://doi:10.1094/pdis-10-17-1684-pdnFariña, A.E., Gorayeb, E.S. y Camelo-García., V.M. (2019). Caracterización molecular y biológica de un nuevo sobemovirus putativo que infecta a Physalis peruviana. Archives Virology 164, 2805–2810.https://doi.org/10.1007/s00705-019-04374-yFereres, A., Perez, P., Gemeno, C. y Ponz, F. (1993). Transmission of Spanish Pepper- and Potato-PVY Isolates by Aphid (Homoptera: Aphididae) Vectors: Epidemiological Implications. Environmental Entomology, 22(6), 1260-1265. https://doi.org/10.1093/ee/22.6.1260Fisher, G., Miranda, D., Romero, J. y Piedrahita, W. (Eds). (2005). Avances en cultivo poscosecha y exportación de la uchuva (Physalis peruviana) en Colombia. Universidad Nacional de Colombia.http://www.asohofrucol.com.co/archivos/biblioteca/biblioteca_23_Avances%20cultivo%20uchuva.pdfFischer, I.H. y Rezende, J.A.M. (2008). Diseases of Passion Flower (Passiflora spp.). Pest Technology 2(1): 1-19. https://www.researchgate.net/publication/228483390_Diseases_of_Passion_flower_Passiflora_sppFischer, G. y Miranda, D. (Eds) (2012). Uchuva (Physalis peruviana L.). Manual para el cultivo de frutas tropicales en el trópico. Produmedios.Fontenele, R. S., Lamas, N. S., Lacorte, C., Lacerda, A. L. M., Varsani, A. y Ribeiro, S. G. (2017). A novel geminivirus identified in tomato and cleome plants sampled in Brazil. Virus Research, 240, 175-179. https://doi:10.1016/j.virusres.2017.08.007Freedman, A. y Weeks, N. (2020). Best Practices for De Novo Transcriptome Assembly with Trinity. Hardvard FAS Informatics. https://informatics.fas.harvard.edu/best-practices-for-de-novo-transcriptome-assembly-with-trinity.htmlFribourg, C.E. (1979). Host plant reactions, some properties, and serology of Peru tomato virus. Phytopathology. 69(5), 441–445.Fribourg, C. E. (1987). A New Tobamovirus from Passiflora edulis in Peru. Phytopathology, 77(3), 486. https://doi.org/10.1094/phyto-77-486Fukumoto, T., Nakamura, M. y Rikitake, M. (2012). Molecular characterization and specific detection of two genetically distinguishable strains of East Asian Passiflora virus (EAPV) and their distribution in southern Japan. Virus Genes, 44, 141-148. https://doi.org/10.1007/s11262-011-0676-7Gallo, Y. M, Jaramillo, H., Toro, L. F., Marín, M. y Gutiérrez, P. A. (2018). Characterization of the genome of a novel ilarvirus naturally infecting Cape gooseberry (Physalis peruviana). Archives of Virology, 163(6), 1713-1716. https://doi.org/10.1007/s00705-018-3796-8Gallo, Y., Montoya, M. M. y Gutiérrez, P. A. (2020). Detection of RNA viruses in Cape gooseberry (Physalis peruviana L.) by RNAseq using total RNA and dsRNA inputs. Archives of Phytopathology and Plant Protection, 53(9-10), 395-413. https://doi.org/10.1080/03235408.2020.1748368Geering, A. D. W. (2021). Badnaviruses (Caulimoviridae) (D. H. Bamford & M. B. T.-E. of V. (Fourth E. Zuckerman (eds.); pp. 158–168). Academic Press. https://doi.org/https://doi.org/10.1016/B978-0-12-814515-9.00147-8Geniza, M. y Jaiswal, P. (2017). Tools for building de novo transcriptome assembly. Current Plant Biology, 11-12, 41-45. https://doi:10.1016/j.cpb.2017.12.004Gergerich, R.C. y Dolja, V. V. (2006). Introducción a los virus vegetales, el enemigo invisible. The Plant Health Instructor. https://doi:10.1094/PHI-I-2008-0122-01Gildow, F. E., Shah, D. A., Sackett, W. M., Butzler, T., Nault, B. A. y Fleischer, S. J. (2008). Transmission efficiency of Cucumber mosaic virus by aphids associated with virus epidemics in snap bean. Phytopathology, 98(11), 1233-1241. https://doi.org/10.1094/PHYTO-98-11-1233Goodwin, S., McPherson, J. D. y McCombie, W. R. (2016). Coming of age: ten years of next-generation sequencing technologies. Nature Reviews Genetics, 17(6), 333–351. https://doi:10.1038/nrg.2016.49Gómez, P., Sempere, R. y Aranda, M. A. (2012). Pepino Mosaic Virus and Tomato Torrado Virus. Viruses and Virus Diseases of Vegetables in the Mediterranean Basin, 505–532. https://doi:10.1016/b978-0-12-394314-9.00014-2González-Garza, R. (2017). Evolución de técnicas de diagnóstico de virus fitopatógenos. Revista Mexicana de fitopatología, 35(3), 591-610. https://doi.org/10.18781/r.mex.fit.1706-1Gordillo, L.A. (2011). Incidencia del Soybean mosaic virus en cultivos de Gulupa (Passiflora edulis Sims) en Cundinamarca y estudio de su diversidad en Colombia. [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/9885Graça, J. V., Trench, T. N. y Martin, M. M. (1985). Tomato spotted wilt virus in commercial Cape gooseberry (Physalis peruviana) in Transkei. Plant Pathology, 34(3), 451–453. https://doi:10.1111/j.1365-3059.1985.tb01390.xGreen, K.J., Chikh-Ali, M., Hamasaki, R.T., Melzer, M.J. y Karasev, A.V.(2017). Potato virus Y (PVY) Isolates from Physalis peruviana are unable to systemically infect Potato or Pepper and form a distinct new lineage Within the PVYC Strain Group. Phytopathology, 107(11):1433-1439. https://doi:10.1094/PHYTO-04-17-0147-RGupta, S.P. y Singh, B.R. (1996). Severe mosaic of Cape gooseberry due to Cucumber mosaic virus. Indian Journal Virology, 24(1), 27-156Hajimorad, M. R., Domier, L. L., Tolin, S. A., Whitham, S. A. y Saghai, M. A. (2018). Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range. Molecular Plant Pathology, 19(7), 1563-1579. http://doi:10.1111/mpp.12644.Hancinsk, R., Mih, D., Mrkvov, M., Candresse, T. y Glasa, M. (2020). Plant viruses infecting Solanaceae family members in the cultivated and wild environments: A Review. Plants, 9, 667. https://doi:10.3390/plants9050667Heredia, S. C. (2016). Erradicación de Onion Yellow Dwarf Virus (OYDV) en cebolla Shallot (Allium cepa var. Aggregatum), mediante el cultivo de meristemas, quimioterapia y termoterapia para la producción de bulbos libres de virus [Tesis de pregrado, Universidad San Francisco de Quito]. Repositorio Digital USFQ. http://repositorio.usfq.edu.ec/handle/23000/5198Hill, J. H. (2001). First Report of Transmission of Soybean mosaic virus and Alfalfa mosaic virus by Aphis glycines in the New World. Plant Disease, 85(5). https://doi.org/10.1094/PDIS.2001.85.5.561C.Horváth, J. (1970). Reaction of Physalis species to plant viruses. The Cape gooseberry as a symptomless carrier of Potato virus X and Y. Acta Phytopathologica, 5, 65-72-Horváth, J. (1996). Ornamental physalis species as perennial virus hosts. Acta Horticulturae, 432, 204-211. https://doi:10.17660/actahortic.1996.432.25Jaramillo, H. (2017). Análisis del transcriptoma y viroma de Passiflora edulis f. edulis en cultivos de Antioquia utilizando métodos de secuenciación de nueva generación [Tesis de maestría, Universidad Nacional de Colombia]. https://repositorio.unal.edu.co/handle/unal/62808Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2018). Molecular characterization of Soybean mosaic virus (SMV) infecting Purple passion fruit (Passiflora edulis f. edulis) in Antioquia, Colombia. Archives of Phytopathology and Plant Protection, 1–20. https://doi:10.1080/03235408.2018.1505411Jaramillo, H., Marín, M. y Gutiérrez, P. A. (2019). Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passion fruit (Passiflora edulis f. edulis). Revista Facultad Nacional de Agronomia Medellín, 72(1), 8643-8654. https://doi.org/10.15446/rfnam.v72n1.69438Jover-Gil, S., Beeri, A., Fresnillo, P., Samach, A. y Candela, H. (2018). Complete genome sequence of a novel virus, classifiable within the Potyviridae family, which infects passion fruit (Passiflora edulis). Archives of Virology. https://doi:10.1007/s00705-018-3983-7Joy, P. P. y Sherin, C.G., (2012). DISEASES OF PASSION FRUIT (Passiflora edulis): Pathogen, Symptoms, Infection, Spread & Management. https://www.researchgate.net/publication/306017818_DISEASES_OF_PASSION_FRUIT_Passiflora_edulis_Pathogen_Symptoms_Infection_Spread_ManagementKreuze, J. Vaira, A. M. Mezel, W. Candresse, T. Zavriev, S. K. Hammond, J. y Ryu, H. (2020). ICTV virus taxonomy profile: Alphaflexiviridae. Journal of General Virology. https://doi.org/10.1099/jgv.0.001436Langmead, B. y Salzberg, S. L. (2012). Fast gapped-read alignment with Bowtie 2. Nature Methods, 9(4), 357–359. https://doi:10.1038/nmeth.1923Lee, J. S., Cho, W.K., Choi, H.-S. y Kim, K.-H. (2011). RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses. The Plant Pathology Journal, 27(3), 291-296. https://doi.org/10.5423/PPJ.2011.27.3.291Legge, A. (1974). Notes on the history, cultivation and uses of Physalis peruviana L. Journal of the Royal Horticultural Society, 99 (7), 310−314.Li, R. y Hartung, J. S. (2007). Reverse transcription-polymerase chain reaction-based detection of plant viruses. Current protocols in microbiology. https://doi.org/10.1002/9780471729259.mc16c01s6Li, H. y Durbin, R. (2010). Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics (Oxford, England), 26(5), 589-595. https://doi.org/10.1093/bioinformatics/btp698Li, H., Zhou, P., Yang, Q., Shen, Y., Deng, J., Li, L. y Zhao, D. (2011). Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis “edulis” and Passiflora edulis “flavicarpa.” Journal of Ethnopharmacology, 133(3), 1085-1090. https://doi:10.1016/j.jep.2010.11.039Li, H., Liu, X., Michaud, J.P., Zhi, H., Li, K., Li, Z. y Li, X. (2018). Host Plant Infection by Soybean mosaic virus Reduces the Fitness of Its Vector, Aphis Glycines (Hemiptera: Aphididae). Journal of Economic Entomology, 111(5). https://doi.org/10.1093/JEE/TOY165.Liberato, J. y Murilo, Z. (s.f.) Diseases of Passionfruit (Passiflora spp.). https://www.apsnet.org/edcenter/resources/commonnames/Pages/Passionfruit.aspxMabele, A. S., Were, H. K., Owuor Ndong’a, M. F. y Mukoye, B. (2021). Occurrence and genetic diversity of groundnut rosette assistor virus in western Kenya. Crop Protection, 139, 105381. https://doi.org/https://doi.org/10.1016/j.cropro.2020.105381Maree, H. J., Fox, A., Al Rwahnih, M., Boonham, N. y Candresse, T. (2018). Application of HTS for Routine Plant Virus Diagnostics: State of the Art and Challenges. Frontiers in Plant Science, 9. https://doi:10.3389/fpls.2018.01082Marín, M. Gutiérrez, P.A. (2016). Principios de virología molecular de plantas tropicales. Agrosavia. http://editorial.agrosavia.co/index.php/publicaciones/catalog/book/69Martínez, M.L. (1998). Revisión de Physalis Sección Epeteiorhiza (Solanaceae). Universidad Nacional Autónoma de México, 69, 71-117. http://www.revistas.unam.mx/index.php/bot/article/view/1909Medford, J. (1992). Vegetative Apical Meristems. Plant Cell, 4, 1029-1039. https://doi.org/10.1105/tpc.4.9.1029Menzel, M. Y. (1951). The Cytotaxonomy and Genetics of Physalis. Proceedings of the American Philosophical Society, 95(2), 132–183. http://www.jstor.org/stable/31433310434.2002.00740.Ministerio de Agricultura y Desarollo Rural [Minagricultura]. (2020). Cadena de pasifloras. https://sioc.minagricultura.gov.co/Pasifloras/Documentos/2020-06-30%20Cifras%20Sectoriales.pdfMink, G. I. (1992). Ilarvirus Vectors BT - Advances in Disease Vector Research (K. F. Harris (ed.); pp. (261–281). Springer New York. https://doi.org/10.1007/978-1-4612-2910-0_8Miranda, D., G. Fischer, C. Carranza, S. Magnitskiy, F. Casierra-Posada, W. Piedrahíta y Flórez, L. (2009). Cultivo, poscosecha y comercialización de las pasifloráceas en Colombia: maracuyá, granadilla, gulupa y curuba. Sociedad Colombiana de Ciencias Hortícolas, Bogotá.http://www.asohofrucol.com.co/archivos/biblioteca/biblioteca_118_cultivo_poscosechavp.pdfMituti, T., Spadotti, D. M. A., Narita, N. y Rezende, J. A. M. (2018). First Report of Sida Mottle Alagoas Virus Infecting Passiflora edulis in Brazil. Plant Disease, 103(1), 169. https://doi.org/10.1094/PDIS-06-18-1068-PDNMorales, F.J., Lozano, I., Castaño, M., Arroyave, J., Velasco, A.C. y Varon, F. (2002). Partial characterization of a tymovirus infecting passion fruit in Colombia, South America. Journal of Phytopathology 150(4-5), 292-296. https://doi:10.1046/j.1439-Morton, J. (1987). Passionfruit. Fruits of warm climates,320–328Mortimer, S. M., Jones, M. G. K., Jones, R. A. C., Thomson, G. y Dwyer, G. I. (2009). A single tube, quantitative real-time RT-PCR assay that detects four potato viruses simultaneously. Journal of Virological Methods, 161(2), 289-296. https://doi:10.1016/j.jviromet.2009.06.027Naidu, R.A. y Hughes, J.D.A. (2001). Methods for the detection of plant virus diseases [conferencia]. Proceedings of a conference on Plant Virology in Sub Saharan Africa, Ibadan, Nigeria, (pp. 233-253). https://hdl.handle.net/10568/96508Nakasato, K., Fujioka, S., Sugawara, Y., Ono, T., Nishio, T. y Tsuda, S. (2020). First detection of two potyviruses, uraria mosaic virus and passiflora mosaic virus Y, from passionfruit in Japan. Journal of General Plant Pathology. https://doi:10.1007/s10327-020-00932-4Nascimento, A., Santana, E. y Braz, A. (2006). Cowpea aphid-borne mosaic virus (CABMV) is widespread in passionfruit in Brazil and causes passionfruit woodiness disease. Archives of Virology 151, 1797–1809.https://doi.org/10.1007/s00705-006-0755-6Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. y Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic acids research, 28(12), E63. https://doi.org/10.1093/nar/28.12.e63Ocampo, J. y Wyckhuys, K. (Eds.) (2012.). Tecnología para el cultivo de la gulupa (Passiflora edulis f. edulis Sims) en Colombia. Centro de Bio-Sistemas de la Universidad Jorge Tadeo Lozano, Centro Internacional de Agricultura Tropical - CIAT y Ministerio de Agricultura y Desarrollo Rural, República de Colombia. https://www.researchgate.net/publication/259946957_Tecnologia_para_el_cultivo_de_la_Gulupa_en_Colombia_Passiflora_edulis_f_edulis_Sims_Purple_Passion_FruitOlivares-Tenorio, M.-L., Dekker, M., Verkerk, R. y Boekel, M. A. J. S. (2016). Health-promoting compounds in cape gooseberry (Physalis peruviana L.): Review from a supply chain perspective. Trends in Food Science & Technology, 57, 83–92. https://doi:10.1016/j.tifs.2016.09.009Panno, S., Matić, S., Tiberini, A., Caruso, A. G., Bella, P., Torta, L., Stassi, R. y Davino, A. S. (2020). Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology. Plants (Basel, Switzerland), 9(4), 461. https://doi.org/10.3390/plants9040461Parrella, G. y Sorrentino, D. (2009). Identification of a Cucumber mosaic virus Isolate from Passiflora edulis in Southern Italy and Validation of Subgroup Identification by In Silico Restriction Fragment Length Polymorphism. Journal of Phytopathology, 157(11–12), 762–767. https://doi.org/10.1111/j.1439-0434.2009.01604.xPássaro, C. (Ed). (2014). Physalis peruviana L: fruta andina para el mundo. http://www.cyted.org/es/noticias/libro-physalis-peruviana-l-fruta-andina-para-el-mundoPavlovic, S., Klaassen, K., Stankovic, B., Stojiljkovic, M. y Zukic, B. (2020). Next-Generation Sequencing: The Enabler and the Way Ahead. Microbiomics, 175–200. https://doi:10.1016/b978-0-12-816664-2.00009-8Perea, M., Rodríguez, N.C., Fisher, G., Velázquez, M., y Mican, Y. (2010). Solanaceae, Biotecnología aplicada al mejoramiento de los cultivos de frutas tropicales. Universidad Nacional de Colombia, 466-490. https://www.uneditorial.com/biotecnologia-aplicada-al-mejoramiento-de-los-cultivos-de-frutas-tropicales-agropecuario.htmlPerry, K. L., Zhang, L. y Palukaitis, P. (1998). Amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the Aphids Myzus persicae and Aphis gossypii. Virology, 242(1), 204–210. https://doi.org/10.1006/viro.1998.8991Prakash, O., Misra, A.K., Singh, S.J. y Srivastava, K.M. (1988). Isolation, purification and electron microscopy of mosaic virus of cape gooseberry. International Journal of Tropical Plant Diseases. 6(1), 85-87.Prammanee, S., Thumjamras, S., Chiemsombat, P. y Pipattanawong, N. (2011). Efficient shoot regeneration from direct apical meristem tissue to produce virus-free purple passion fruit plants. Crop Protection, 30(11), 1425-1429. https://doi:10.1016/j.cropro.2011.07.008PROCOLOMBIA. (s.f.). Uchuva (goldenberry). https://docs.procolombia.co/int-procolombia/es/exportaciones/ficha_uchuva_final.pdfPROCOLOMBIA. (2021). Aumentan los pedidos de frutas colombianas en Europa. https://procolombia.co/noticias/aumentan-los-pedidos-de-frutas-colombianas-en-europaPuente, L. A., Pinto-Muñoz, C. A., Castro, E. S. y Cortés, M. (2011). Physalis peruviana Linnaeus, the multiple properties of a highly functional fruit: A review. Food Research International, 44(7), 1733-1740. https://doi.org/https://doi.org/10.1016/j.foodres.2010.09.034Reuter, J. A., Spacek, D. V. y Snyder, M. P. (2015). High-Throughput Sequencing Technologies. Molecular Cell, 58(4), 586-597. https://doi:10.1016/j.molcel.2015.05.004Salamon, P. y Palkovics, L. (2005). Occurrence of colombian datura virus in Brugmansia hybrids, Physalis peruviana L. and Solanum muricatum Ait. in Hungary. Acta Virologica. 49, 117-122Sepúlveda, M., Cardona, D., Gallo, Y., Higuita, M., Gutiérrez, P. y Marín, M. (2022). Virome analysis for identification of viruses associated with asymptomatic infection of purple passion fruit (Passiflora edulis f. edulis) in Colombia, The Journal of Horticultural Science and Biotechnology, 1–14. https://doi: 10.1080/14620316.2021.1973583Vaca-Vaca, J.C., Carrasco-Lozano, E.C. y López-López, K. (2017). Molecular identification of a new begomovirus infecting yellow passion fruit (Passiflora edulis) in Colombia. Archives of Virology 162, 573–576. https://doi.org/10.1007/s00705-016-3098-yAgindotan, B.O., P.J. Shiel, P.H. Berger. 2007. Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan real-time RT-PCR. Journal of Virological Methods 142(1-2): 1-9. https://doi: 10.1016/j.jviromet.2006.12.012.Agronet, 2020. Red de información y comunicación del sector Agropecuario Colombiano. https://www.agronet.gov.co/estadistica/Paginas/home.aspx (accessed 20 september 2021).Álvarez, N., H. Jaramillo, Y. Gallo, P. Gutiérrez, M. Marín. 2018. Molecular characterization of Potato virus Y (PVY) and Potato virus V (PVV) isolates naturally infecting Cape gooseberry (Physalis peruviana) in Antioquia, Colombia. Agronomía Colombiana 36(1): 13–23. https://dx.doi.org/10.15446/agron.colomb.v36n1.65051Ayala, M., P. González, P. Gutiérrez, J. Cotes, M. Marín. 2010. Caracterización serológica y molecular de potyvirus asociados a la virosis del tomate de árbol en Antioquia (Colombia). Acta Biológica Colombiana 15: 143-162.Brewer, E., M. Cao, B. Gutierrez, M. Bateman, R. Li. 2020. Discovery and molecular characterization of a novel trichovirus infecting sweet cherry. Virus Genes 56: 380-385. https://doi.org/10.1007/s11262-020-01743-7Bushmanova, E., D. Antipov, A. Lapidus, A.D. Prjibelski. 2019. rnaSPAdes: a de novo transcriptome assembler and its application to RNA-Seq data, GigaScience 8(9): 1-13. https://doi.org/10.1093/gigascience/giz100Duarte, P.D.S.G., S.B.F. Galvino-Costa, S.R.R. de Paula Ribeiro, A.R. Figueira. 2012. Complete genome sequence of the first Andean strain of potato virus S from Brazil and evidence of recombination between PVS strains. Archives of Virology 157: 1357–1364. https://doi.org/10.1007/s00705-012-1289-8Duque, M., M. Marín, P.A. Gutiérrez. 2017. Genome comparison and primer design for detection of Tamarillo leaf malformation virus (TaLMV). Archives of Phytopathology and Plant Protection 50: 713-726. https://doi.org/10.1080/03235408.2017.1370934Edgar, R.C., R.M. Drive, M. Valley. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340.Fischer, G., L.M. Melgarejo. 2020. The ecophysiology of cape gooseberry (Physalis peruviana L.) - an Andean fruit crop. A review. Revista Colombiana de Ciencias Hortícolas 14(1): 76-89. https://doi.org/10.17584/rcch.2020v14i1.10893Fuchs, M., C. Schmitt-Keichinger, H. Sanfaçon. 2017. A renaissance in Nepovirus research provides new insights into their molecular interface with hosts and vectors. Advances in Virus Research 97: 61-105. https://doi.org/10.1016/bs.aivir.2016.08.009Gallo, Y. 2020. Caracterización molecular del viroma de plantas solanáceas de importancia económica en Antioquia. PhD. thesis Biotechnology. Universidad Nacional de Colombia sede Medellín.García, N., P. Gutiérrez, M. Marín. 2013. Detección y cuantificación del Potato mop-top virus (PMTV) en Colombia mediante qRT-PCR. Acta Agronómica 62(2): 120-128.Gil, J.F., J.M. Cotes, E.P. González, M. Marín. 2011. Caracterización genotípica de aislamientos colombianos del potato mop-top virus (PMTV, Pomovirus). Actualidades Biológicas 33(94): 69–84.Gil, J.F., I. Adams, N. Boonham, S.L. Nielsen, M. Nicolaisen. 2016. Molecular and biological characterization of Potato mop-top virus (PMTV, Pomovirus) isolates from the potato-growing regions of Colombia. Plant Pathology 65: 1210–1220. https://doi.org/10.1111/ppa.12491.Gish, W., D.J. States. 1993. Identification of protein coding regions by database similarity search. Nature Genetics 3: 266–272. https://doi.org/10.1038/ng0393-266Gutiérrez, P.A., J.F. Alzate, M.M. Montoya. 2015a. Complete genome sequence of an isolate of Potato virus X (PVX) infecting cape gooseberry (Physalis peruviana) in Colombia. Virus Genes 50(3): 518-522. https://10.1007/s11262-015-1181-1Gutiérrez, P.A., J.F. Alzate, M. Marín. 2015b. Genome sequence of a virus isolate from tamarillo (Solanum betaceum) in Colombia: evidence for a new potyvirus. Archives of Virology 160(2): 557-560. https://doi.org/10.1007/s00705-014-2296-8Gutiérrez, P., A. Rivillas, D. Tejada, S. Giraldo, A. Restrepo, M. Ospina, S. Cadavid, Y. Gallo, M. Marín. 2021. PVDP: A portable open source pipeline for detection of plant viruses in RNAseq data. A case study on potato viruses in Antioquia (Colombia). Physiological and Molecular Plant Pathology 113: 101604. https://doi.org/10.1016/j.pmpp.2021.101604Hull, R. 2013. Plant Virology. 5th Edition. Academic Press, London. 918 p.Johansen, E., M.C. Edwards, R.O. Hampton. 1994. Seed transmission of viruses: Current Perspectives. Annual Review of Phytopathology 32: 363-386.Jones, D.T., W.R. Taylor, J.M. Thornton. 1992. The rapid generation of mutation data matrices from protein sequences. Computer Applications in the Biosciences 8: 275-282. https://doi.org/10.1093/bioinformatics/8.3.275Kim, S., M. Park, S.I. Yeom, Y.M. Kim, J.M. Lee, H.A. Lee, E. Seo, J. Choi, K. Cheong, K.T. Kim, K. Jung, G.W. Lee, S.K. Oh, C. Bae, S.B. Kim, H.Y. Lee, S.Y. Kim, M.S. Kim, B.C. Kang, Y.D. Jo, H.B. Yang, H.J. Jeong, W.H. Kang, J.K. Kwon, C. Shin, J.Y. Lim, J.H. Park, J.H. Huh, J.S. Kim, B.D. Kim, O. Cohen, I. Paran, M.C. Suh, S.B. Lee, Y.K. Kim, Y. Shin, S.J. Noh, J. Park, Y.S. Seo, S.Y. Kwon, H.A. Kim, J.M. Park, H.J. Kim, S.B. Choi, P.W. Bosland, G. Reeves, S.H. Jo, B.W. Lee, H.T. Cho, H.S. Choi, M.S. Lee, Y. Yu, Y. Do Choi, B.S. Park, A. van Deynze, H. Ashrafi, T. Hill, W.T. Kim, H.S. Pai, H.K. Ahn, I. Yeam, J.J. Giovannoni, J.K. Rose, I. Sørensen, S.J. Lee, R.W. Kim, I.Y. Choi, B.S. Choi, J.S. Lim, Y.H. Lee, D. Choi. 2014. Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species. Nature Genetics 2014 46(3): 270-278. https://doi.org/10.1038/ng.2877King, A.M.Q. 2012. Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego.Kitajima, E.W. 2020. An annotated list of plant viruses and viroids described in Brazil (1926-2018). Biota Neotropica 20(2): e20190932. https://doi.org/10.1590/1676-0611-BN-2019-0932.Kumar, S., G. Stecher, M. Li, C. Knyaz, K. Tamura. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547-1549. https://doi.org/10.1093/molbev/msy096.Martelli, G.P., T. Candresse, S. Namba. 1994. Trichovirus, a new genus of plant viruses. Archives of Virology 134(3-4): 451-455. https://doi.org/10.1007/BF01310583.Mistry, J., S. Chuguransky, L. Williams, M. Qureshi, G.A. Salazar, E.L.L. Sonnhammer, S.C.E. Tosatto, L. Paladin, S. Raj, L.J. Richardson, R.D. Finn, A. Bateman. 2021. Pfam: The protein families database in 2021. Nucleic Acids Research, 49(D1): D412-D419. https://doi.org/10.1093/nar/gkaa913Posada, D., K.A. Crandall. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817-818. https://doi.org/10.1093/bioinformatics/14.9.817Procolombia. 2020. Uchuva (Goldenberry). https://docs.procolombia.co/int-procolombia/es/exportaciones/ficha_uchuva_final.pdf (accessed 10 October 2021)Saitou, N., M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4(4): 406–425. https://10.1093/oxfordjournals.molbev.a040454Savenkov, E.I., M.Y. Sandgren, J.P.T. Valkonen. 1999. Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus. Journal of General Virology 80: 2779-2784. https://10.1099/0022-1317-80-10-2779Singh, M., Singh, R.P., Fageria, M.S., Nie, X., Coffin, R., Hawkins, G. 2013. Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers. American Journal of Potato Research, 90: 43–50. https://doi.org/10.1007/s12230-012-9274-zTamura K. 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Molecular Biology and Evolution 9(4): 678-687. https://10.1093/oxfordjournals.molbev.a040752Vallejo, D., Gutiérrez, P., M. Marín. 2016. Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk. Agronomía Colombiana 34: 51–60. https://doi.org/10.15446/agron.colomb.v34n1.53161.Agronet (2021). Área, producción y rendimiento de Gulupa en Colombia. In: https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1; accessed: October 2020.Bhat AI, Hohn T, Selvarajan R (2016). Badnaviruses: The current global scenario. Viruses 8(6): 177. doi: 10.3390/v8060177Bushmanova E, Antipov D, Lapidus A, Prjibelski AD (2019) rnaSPAdes: a de novo transcriptome assembler and its application to RNA-Seq data. Gigascience 8(9): giz100. doi: 10.1093/gigascience/giz100Cardona D, Higuita M, Posada J, Gallo Y, Marín M, Gutiérrez P (2022a). Viruses infecting purple passion fruit (Passiflora edulis f. edulis) in Southwestern Antioquia (Colombia). Archives of Phytopathology and Plant Protection. Archives of Phytopathology and Plant Protection, 55(12): 1394–1409. doi: 10.1080/03235408.2022.2098588Cardona, D., Restrepo, A., Higuita, M., Gallo, Y., Marin, M., y Gutiérrez, P. (2022b). Natural infection of purple passion fruit (Passiflora edulis f. edulis) by a novel member of the family Tymoviridae in Colombia. Acta virologica, 66(3):254–262. doi:10.4149/av_2022_310Cardona D, Gallo Y, Higuita M, Hoyos R, Gutiérrez P, Marín M (2022c). Detección molecular de virus en cultivos, plántulas y semillas de gulupa (Passiflora edulis f. edulis) en el oriente de Antioquia. Bioagro: 34(2).Chabannes M, Gabriel M, Aksa A, Galzi S, Dufayard JF, Iskra-Caruana ML, Muller E (2020). Badnaviruses and banana genomes: a long association sheds light on Musa phylogeny and origin. Molecular Plant Pathology 22:216-230. doi: 10.1111/mpp.13019Chomczynski P, Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162(1): 156-159. doi: 10.1006/abio.1987.9999Cuspoca, J (2007). Evaluación de virus de tomate de árbol (Solanum betaceum) en plantas indicadoras y su detección por PCR. Thesis in Agronomy. Faculty of Agronomy. Universidad Nacional de Colombia. Bogotá. 31 p.Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.Feng JL, Chen SN, Tang XS, Ding XF, Du ZY, Chen JS (2006). Quantitative determination of cucumber mosaic virus genome RNAs in virions by Real-Time Reverse Transcription-Polymerase Chain Reaction. Acta Biochimica et Biophysica Sinica, 38(10): 669-676. doi: 10.1111/j.1745-7270.2006.00216.xGao L, Ding X, Li K, Liao W, Zhong Y, Ren R, Liu Z, Adhimoolam K, Zhi H (2015). Characterization of Soybean mosaic virus resistance derived from inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars. Theoretical and Applied Genetics, 128: 1489-1505. doi: 10.1007/s00122-015-2522-0Gillaspie AG Jr, Pio-Ribeiro G, Andrade GP, Pappu HR (2001). RT-PCR Detection of seedborne Cowpea aphid-borne mosaic virus in peanut. Plant Disease, 85(11): 1181-1182. doi: 10.1094/PDIS.2001.85.11.1181Gonçalves Z, de Jesus O, Cerqueira-Silva CB; Diniz RP, Soares TL, de Oliveira EJ (2017). Methodological approaches to assess passion fruit resistance (Passiflora spp.) to passionfruit woodiness disease. Bioscience Journal, 33(6): 1441-1451. doi: 10.14393/BJ-v33n6a2017-36619Gordillo LA (2011). Incidencia del Soybean mosaic virus en cultivos de Gulupa (Passiflora edulis Sims) en Cundinamarca y estudio de su diversidad en Colombia. Master’s Thesis in Microbiology. Faculty of Sciences. Universidad Nacional de Colombia. Bogotá. 106 p. https://repositorio.unal.edu.co/handle/unal/9885Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS (2018). UFBoot2: Improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution 35(2): 518-522. doi: 10.1093/molbev/msx281. PMID: 29077904Jaramillo H, Marín M and Gutiérrez P (2018). Molecular characterization of Soybean mosaic virus (SMV) infecting Purple passion fruit (Passiflora edulis f. edulis) in Antioquia, Colombia. Archives of Phytopathology and Plant Protection, 51(11-12): 617-636. doi: 10.1080/03235408.2018.1505411Jaramillo H, Marín M and Gutiérrez P (2019). Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passion fruit (Passiflora edulis f. edulis). Revista Facultad Nacional de Agronomía Medellín, 72(1): 8643-8654. doi: 10.15446/rfnam.v72n1.69438Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS (2017). ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14(6): 587-589. doi: 10.1038/nmeth.4285Katoh K, Standley DM (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4): 772-780. doi: 10.1093/molbev/mst010King AMQ (2012). Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego.King AMQ (2012). Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press. San Diego.Melgarejo, L, Fischer, G, Cuca Suárez, L, Hernández Gómez, M, Hoyos Carvajal, L, Magnitskiy, S, Brochero, H, Miranda Lasprilla, D, Álvarez-Flórez, F, Ávila Murillo, M, Delgado Ávila, W, Mendoza Forero, C, Lizarazo Hernández, K, Solarte Cruz, M, Hurtado Clopatosky, S, Plazas Rodríguez, E, García Morantes, J, Ramírez Soler, C, Márquez-Niño, F, Moreno Echeverry, D, Sandoval, J, Flechas Bejarano, N, Díaz Ardila, H, Cárdenas Pira, W, Torres Moya, E, Cruz Ospina, S, Toro Tobón, G, Paz Figueroa, V y Rodríguez Castillo, N. (2019). Gulupa (Passiflora edulis), curuba (Passiflora tripartita), aguacate (Persea americana) y tomate de árbol (Solanum betaceum) Innovaciones. Universidad Nacional de Colombia, Bogotá. https://repositorio.unal.edu.co/handle/unal/79864Munguti F, Maina S, Nyaboga EN, Kilalo D, Kimani E, Macharia M, Holton T (2019). Transcriptome sequencing reveals a complete genome sequence of cowpea aphid-borne mosaic virus from passion fruit in Kenya. Microbiology Resource Announcements, 8(2): e01607-18. doi: 10.1128/MRA.01607-18.Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ (2015). IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32(1): 268-274. doi: 10.1093/molbev/msu300Robinson JT, Thorvaldsdóttir H, Winckler W, Guttman M, Lander ES, Getz G, Mesirov JP (2011). Integrative Genomics Viewer. Nature Biotechnology 29(1): 24-26. doi: 10.1038/nbt.1754Xanthis CK, Maliogka VI, Lecoq H, Dezbiez C, Tsvetkov I, Katis NI (2015). First report of cucumber mosaic virus infecting watermelon in Greece and Bulgaria. Journal of Plant Pathology 97(2): 391-403. doi: 10.4454/JPP.V97I2.00(Proyecto 1101-805-62786, Convenio No. 4600007658-779) " Detección, caracterización y limpieza de virus y otros fitopatógenos en material de siembra y cultivos de gulupa (Passiflora edulis f. edulis) y uchuva (Physalis peruviana) en Antioquia"Fondo de Ciencia, Tecnología e Innovación del Sistema General de Regalías del Departamento de AntioquiaAdministradoresBibliotecariosConsejerosEstudiantesGrupos comunitariosInvestigadoresMaestrosMedios de comunicaciónPadres y familiasPersonal de apoyo escolarProveedores de ayuda financiera para estudiantesPúblico generalReceptores de fondos federales y solicitantesResponsables políticosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83420/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53CC-LICENSEBacca David Michelle.licencia.cap.1.pdfBacca David Michelle.licencia.cap.1.pdfapplication/pdf268312https://repositorio.unal.edu.co/bitstream/unal/83420/10/Bacca%20David%20Michelle.licencia.cap.1.pdfc35d6e5beb2a767ae474f81fe798a13eMD510Bacca David Michelle.licencia.cap.2.pdfBacca David Michelle.licencia.cap.2.pdfapplication/pdf270265https://repositorio.unal.edu.co/bitstream/unal/83420/11/Bacca%20David%20Michelle.licencia.cap.2.pdfc9035d234e07cb41695ae39f2571781cMD511Bacca David Michelle.licencia.cap.3.pdfBacca David Michelle.licencia.cap.3.pdfapplication/pdf268832https://repositorio.unal.edu.co/bitstream/unal/83420/12/Bacca%20David%20Michelle.licencia.cap.3.pdfcf3c64bfc0801fe7794a600bebaa8495MD512Bacca David Michelle.licencia.cap.4.pdfBacca David Michelle.licencia.cap.4.pdfapplication/pdf269409https://repositorio.unal.edu.co/bitstream/unal/83420/13/Bacca%20David%20Michelle.licencia.cap.4.pdf9fd00672bff9949b5b7888a8194cf317MD513Bacca David Michelle.licencia.cap.5.pdfBacca David Michelle.licencia.cap.5.pdfapplication/pdf254659https://repositorio.unal.edu.co/bitstream/unal/83420/14/Bacca%20David%20Michelle.licencia.cap.5.pdff2f8bad1d19cbc2eaf191840a1f9e13aMD514ORIGINAL1037654739.2022.pdf1037654739.2022.pdfTesis de maestría en Ciencias- Biotecnologíaapplication/pdf4193475https://repositorio.unal.edu.co/bitstream/unal/83420/4/1037654739.2022.pdf5c4479b71731f63429b9a674dae6a144MD54THUMBNAIL1037654739.2022.pdf.jpg1037654739.2022.pdf.jpgGenerated Thumbnailimage/jpeg4891https://repositorio.unal.edu.co/bitstream/unal/83420/15/1037654739.2022.pdf.jpgdd4dcd7a90e0512116642cdda0d3aa89MD515unal/83420oai:repositorio.unal.edu.co:unal/834202023-10-04 11:40:49.606Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.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

Caracterización molecular de virus en cultivos de uchuva (Physalis peruviana) y gulupa (Passiflora edulis f. edulis) en el suroeste de Antioquia

Publicaciones similares