Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
To determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) isolated in establishments that commercialize raw ground beef and pork chops in Cartagena- Colombia. 160 samples were analyzed through microbiological cultures in Baire Parcker agar, and it was determined the presence...
- Autores:
-
López Gutierrez, Lersy
Bettin Martinez, Alfonso
Suárez Mahecha, Héctor
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/66000
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/66000
http://bdigital.unal.edu.co/67023/
- Palabra clave:
- 63 Agricultura y tecnologías relacionadas / Agriculture
MRSA
MSSA
PCR
Panton Valentine Leukocidin toxin (PVL)
Meat products
Bacterial resistance
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López Gutierrez, Lersycf19b401-983a-4aa5-bbb5-cf0f6417bdfb300Bettin Martinez, Alfonso45655485-6335-429e-bd6e-f0a1831c4563300Suárez Mahecha, Héctord5554d2c-4162-4e7c-b868-c2f802438d173002019-07-03T01:18:13Z2019-07-03T01:18:13Z2017-01-01ISSN: 2248-7026https://repositorio.unal.edu.co/handle/unal/66000http://bdigital.unal.edu.co/67023/To determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) isolated in establishments that commercialize raw ground beef and pork chops in Cartagena- Colombia. 160 samples were analyzed through microbiological cultures in Baire Parcker agar, and it was determined the presence of mecA gen that codifies the methicillin resistance and the pvl that codifies the Panton- Valentine leukocidin toxin (PVL) by the multiplex PCR technique. The antibiotic susceptibility profile for MRSA strains was realized by automatized methods and for MSSA strains it was used Kirby Bauver. 66 samples were confirmed as S. aureus by PCR. The prevalence of MRSA was 7.5% and 33.8% of MSSA. The 66% of the strains were isolated from raw ground beef and the 34% of pork chop meat. The isolations presented about 2 – 12% of multi-resistance to the antibiotics used. The MRSA showed resistance to amoxicillin- clavulanate (57%), ampicillin-sulbactam and cefazolin (85%), erythromycin and clindamycin (7%), tetracycline (35%). The 10% of the isolated strains had the gen of PVL toxin and the 71% of those were identified in samples of raw pork meat and the 28% in raw ground beef. This study reports for the first time, how meat raw products commercialized in the city of Cartagena could build a dissemination source of MRSA carrier of PVL toxin that could generate a public health disease.application/pdfspaUniversidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrariashttps://revistas.unal.edu.co/index.php/refame/article/view/61768Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía MedellínRevista Facultad Nacional de Agronomía MedellínLópez Gutierrez, Lersy and Bettin Martinez, Alfonso and Suárez Mahecha, Héctor (2017) Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia. Revista Facultad Nacional de Agronomía Medellín, 70 (1). pp. 8091-8098. ISSN 2248-702663 Agricultura y tecnologías relacionadas / AgricultureMRSAMSSAPCRPanton Valentine Leukocidin toxin (PVL)Meat productsBacterial resistanceMethicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, ColombiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL61768-313974-2-PB.pdfapplication/pdf395936https://repositorio.unal.edu.co/bitstream/unal/66000/1/61768-313974-2-PB.pdf61582e9d55451dafeae7ff2c09455ea4MD51THUMBNAIL61768-313974-2-PB.pdf.jpg61768-313974-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg8278https://repositorio.unal.edu.co/bitstream/unal/66000/2/61768-313974-2-PB.pdf.jpg0e3ce393d5523c1af5f1dd3a5b739b02MD52unal/66000oai:repositorio.unal.edu.co:unal/660002024-05-13 23:08:59.859Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia |
title |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia |
spellingShingle |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia 63 Agricultura y tecnologías relacionadas / Agriculture MRSA MSSA PCR Panton Valentine Leukocidin toxin (PVL) Meat products Bacterial resistance |
title_short |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia |
title_full |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia |
title_fullStr |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia |
title_full_unstemmed |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia |
title_sort |
Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia |
dc.creator.fl_str_mv |
López Gutierrez, Lersy Bettin Martinez, Alfonso Suárez Mahecha, Héctor |
dc.contributor.author.spa.fl_str_mv |
López Gutierrez, Lersy Bettin Martinez, Alfonso Suárez Mahecha, Héctor |
dc.subject.ddc.spa.fl_str_mv |
63 Agricultura y tecnologías relacionadas / Agriculture |
topic |
63 Agricultura y tecnologías relacionadas / Agriculture MRSA MSSA PCR Panton Valentine Leukocidin toxin (PVL) Meat products Bacterial resistance |
dc.subject.proposal.spa.fl_str_mv |
MRSA MSSA PCR Panton Valentine Leukocidin toxin (PVL) Meat products Bacterial resistance |
description |
To determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) isolated in establishments that commercialize raw ground beef and pork chops in Cartagena- Colombia. 160 samples were analyzed through microbiological cultures in Baire Parcker agar, and it was determined the presence of mecA gen that codifies the methicillin resistance and the pvl that codifies the Panton- Valentine leukocidin toxin (PVL) by the multiplex PCR technique. The antibiotic susceptibility profile for MRSA strains was realized by automatized methods and for MSSA strains it was used Kirby Bauver. 66 samples were confirmed as S. aureus by PCR. The prevalence of MRSA was 7.5% and 33.8% of MSSA. The 66% of the strains were isolated from raw ground beef and the 34% of pork chop meat. The isolations presented about 2 – 12% of multi-resistance to the antibiotics used. The MRSA showed resistance to amoxicillin- clavulanate (57%), ampicillin-sulbactam and cefazolin (85%), erythromycin and clindamycin (7%), tetracycline (35%). The 10% of the isolated strains had the gen of PVL toxin and the 71% of those were identified in samples of raw pork meat and the 28% in raw ground beef. This study reports for the first time, how meat raw products commercialized in the city of Cartagena could build a dissemination source of MRSA carrier of PVL toxin that could generate a public health disease. |
publishDate |
2017 |
dc.date.issued.spa.fl_str_mv |
2017-01-01 |
dc.date.accessioned.spa.fl_str_mv |
2019-07-03T01:18:13Z |
dc.date.available.spa.fl_str_mv |
2019-07-03T01:18:13Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.issn.spa.fl_str_mv |
ISSN: 2248-7026 |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/66000 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/67023/ |
identifier_str_mv |
ISSN: 2248-7026 |
url |
https://repositorio.unal.edu.co/handle/unal/66000 http://bdigital.unal.edu.co/67023/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/refame/article/view/61768 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía Medellín Revista Facultad Nacional de Agronomía Medellín |
dc.relation.references.spa.fl_str_mv |
López Gutierrez, Lersy and Bettin Martinez, Alfonso and Suárez Mahecha, Héctor (2017) Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia. Revista Facultad Nacional de Agronomía Medellín, 70 (1). pp. 8091-8098. ISSN 2248-7026 |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrarias |
institution |
Universidad Nacional de Colombia |
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