Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia

To determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) isolated in establishments that commercialize raw ground beef and pork chops in Cartagena- Colombia. 160 samples were analyzed through microbiological cultures in Baire Parcker agar, and it was determined the presence...

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Autores:
López Gutierrez, Lersy
Bettin Martinez, Alfonso
Suárez Mahecha, Héctor
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/66000
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/66000
http://bdigital.unal.edu.co/67023/
Palabra clave:
63 Agricultura y tecnologías relacionadas / Agriculture
MRSA
MSSA
PCR
Panton Valentine Leukocidin toxin (PVL)
Meat products
Bacterial resistance
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López Gutierrez, Lersycf19b401-983a-4aa5-bbb5-cf0f6417bdfb300Bettin Martinez, Alfonso45655485-6335-429e-bd6e-f0a1831c4563300Suárez Mahecha, Héctord5554d2c-4162-4e7c-b868-c2f802438d173002019-07-03T01:18:13Z2019-07-03T01:18:13Z2017-01-01ISSN: 2248-7026https://repositorio.unal.edu.co/handle/unal/66000http://bdigital.unal.edu.co/67023/To determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) isolated in establishments that commercialize raw ground beef and pork chops in Cartagena- Colombia. 160 samples were analyzed through microbiological cultures in Baire Parcker agar, and it was determined the presence of mecA gen that codifies the methicillin resistance and the pvl that codifies the Panton- Valentine leukocidin toxin (PVL) by the multiplex PCR technique. The antibiotic susceptibility profile for MRSA strains was realized by automatized methods and for MSSA strains it was used Kirby Bauver. 66 samples were confirmed as S. aureus by PCR. The prevalence of MRSA was 7.5% and 33.8% of MSSA. The 66% of the strains were isolated from raw ground beef and the 34% of pork chop meat. The isolations presented about 2 – 12% of multi-resistance to the antibiotics used. The MRSA showed resistance to amoxicillin- clavulanate (57%), ampicillin-sulbactam and cefazolin (85%), erythromycin and clindamycin (7%), tetracycline (35%). The 10% of the isolated strains had the gen of PVL toxin and the 71% of those were identified in samples of raw pork meat and the 28% in raw ground beef. This study reports for the first time, how meat raw products commercialized in the city of Cartagena could build a dissemination source of MRSA carrier of PVL toxin that could generate a public health disease.application/pdfspaUniversidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrariashttps://revistas.unal.edu.co/index.php/refame/article/view/61768Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía MedellínRevista Facultad Nacional de Agronomía MedellínLópez Gutierrez, Lersy and Bettin Martinez, Alfonso and Suárez Mahecha, Héctor (2017) Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia. Revista Facultad Nacional de Agronomía Medellín, 70 (1). pp. 8091-8098. ISSN 2248-702663 Agricultura y tecnologías relacionadas / AgricultureMRSAMSSAPCRPanton Valentine Leukocidin toxin (PVL)Meat productsBacterial resistanceMethicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, ColombiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL61768-313974-2-PB.pdfapplication/pdf395936https://repositorio.unal.edu.co/bitstream/unal/66000/1/61768-313974-2-PB.pdf61582e9d55451dafeae7ff2c09455ea4MD51THUMBNAIL61768-313974-2-PB.pdf.jpg61768-313974-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg8278https://repositorio.unal.edu.co/bitstream/unal/66000/2/61768-313974-2-PB.pdf.jpg0e3ce393d5523c1af5f1dd3a5b739b02MD52unal/66000oai:repositorio.unal.edu.co:unal/660002024-05-13 23:08:59.859Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
title Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
spellingShingle Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
63 Agricultura y tecnologías relacionadas / Agriculture
MRSA
MSSA
PCR
Panton Valentine Leukocidin toxin (PVL)
Meat products
Bacterial resistance
title_short Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
title_full Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
title_fullStr Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
title_full_unstemmed Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
title_sort Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia
dc.creator.fl_str_mv López Gutierrez, Lersy
Bettin Martinez, Alfonso
Suárez Mahecha, Héctor
dc.contributor.author.spa.fl_str_mv López Gutierrez, Lersy
Bettin Martinez, Alfonso
Suárez Mahecha, Héctor
dc.subject.ddc.spa.fl_str_mv 63 Agricultura y tecnologías relacionadas / Agriculture
topic 63 Agricultura y tecnologías relacionadas / Agriculture
MRSA
MSSA
PCR
Panton Valentine Leukocidin toxin (PVL)
Meat products
Bacterial resistance
dc.subject.proposal.spa.fl_str_mv MRSA
MSSA
PCR
Panton Valentine Leukocidin toxin (PVL)
Meat products
Bacterial resistance
description To determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) isolated in establishments that commercialize raw ground beef and pork chops in Cartagena- Colombia. 160 samples were analyzed through microbiological cultures in Baire Parcker agar, and it was determined the presence of mecA gen that codifies the methicillin resistance and the pvl that codifies the Panton- Valentine leukocidin toxin (PVL) by the multiplex PCR technique. The antibiotic susceptibility profile for MRSA strains was realized by automatized methods and for MSSA strains it was used Kirby Bauver. 66 samples were confirmed as S. aureus by PCR. The prevalence of MRSA was 7.5% and 33.8% of MSSA. The 66% of the strains were isolated from raw ground beef and the 34% of pork chop meat. The isolations presented about 2 – 12% of multi-resistance to the antibiotics used. The MRSA showed resistance to amoxicillin- clavulanate (57%), ampicillin-sulbactam and cefazolin (85%), erythromycin and clindamycin (7%), tetracycline (35%). The 10% of the isolated strains had the gen of PVL toxin and the 71% of those were identified in samples of raw pork meat and the 28% in raw ground beef. This study reports for the first time, how meat raw products commercialized in the city of Cartagena could build a dissemination source of MRSA carrier of PVL toxin that could generate a public health disease.
publishDate 2017
dc.date.issued.spa.fl_str_mv 2017-01-01
dc.date.accessioned.spa.fl_str_mv 2019-07-03T01:18:13Z
dc.date.available.spa.fl_str_mv 2019-07-03T01:18:13Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 2248-7026
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/66000
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/67023/
identifier_str_mv ISSN: 2248-7026
url https://repositorio.unal.edu.co/handle/unal/66000
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dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/refame/article/view/61768
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía Medellín
Revista Facultad Nacional de Agronomía Medellín
dc.relation.references.spa.fl_str_mv López Gutierrez, Lersy and Bettin Martinez, Alfonso and Suárez Mahecha, Héctor (2017) Methicillin resistant Staphylococcus aureus isolated from meat raw in Cartagena, Colombia. Revista Facultad Nacional de Agronomía Medellín, 70 (1). pp. 8091-8098. ISSN 2248-7026
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrarias
institution Universidad Nacional de Colombia
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