Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo

ilustraciones, gráficas, tablas

Autores:: López González, David Alejandro

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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oai_identifier_str	oai:repositorio.unal.edu.co:unal/80620
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo
dc.title.translated.eng.fl_str_mv	Effect of IGF2 on the DNA methylation landscape and associated mRNA expression in HTR-8/SVneo cell line
title	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo
spellingShingle	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo 570 - Biología::572 - Bioquímica Oncogenes Metilación de ADN Receptor IGF Tipo 2 Oncogenes DNA Methylation Receptor, IGF Type 2 Cáncer Metilación del DNA Placenta Factor de crecimiento Epigenética Cancer IGF signaling system Epigenetics Genomic instability Genic expression Malignant transformation
title_short	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo
title_full	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo
title_fullStr	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo
title_full_unstemmed	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo
title_sort	Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo
dc.creator.fl_str_mv	López González, David Alejandro
dc.contributor.advisor.spa.fl_str_mv	Umaña Pérez, Yadi Adriana
dc.contributor.author.spa.fl_str_mv	López González, David Alejandro
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Hormonas
dc.subject.ddc.spa.fl_str_mv	570 - Biología::572 - Bioquímica
topic	570 - Biología::572 - Bioquímica Oncogenes Metilación de ADN Receptor IGF Tipo 2 Oncogenes DNA Methylation Receptor, IGF Type 2 Cáncer Metilación del DNA Placenta Factor de crecimiento Epigenética Cancer IGF signaling system Epigenetics Genomic instability Genic expression Malignant transformation
dc.subject.decs.spa.fl_str_mv	Oncogenes Metilación de ADN Receptor IGF Tipo 2
dc.subject.decs.eng.fl_str_mv	Oncogenes DNA Methylation Receptor, IGF Type 2
dc.subject.proposal.spa.fl_str_mv	Cáncer Metilación del DNA Placenta Factor de crecimiento Epigenética
dc.subject.proposal.eng.fl_str_mv	Cancer IGF signaling system Epigenetics Genomic instability Genic expression Malignant transformation
description	ilustraciones, gráficas, tablas
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-10-27T14:11:50Z
dc.date.available.none.fl_str_mv	2021-10-27T14:11:50Z
dc.date.issued.none.fl_str_mv	2021-07-28
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/80620
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/80620 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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spelling	Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Umaña Pérez, Yadi Adrianad652013090d46425f836051252c18e62López González, David Alejandro4fecb1c9885cec0383419bbf14e3e6d7Grupo de Investigación en Hormonas2021-10-27T14:11:50Z2021-10-27T14:11:50Z2021-07-28https://repositorio.unal.edu.co/handle/unal/80620Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficas, tablasEl proceso de implantación placentaria depende de una minuciosa regulación de la invasión de la decidua materna por parte del subtipo celular denominado trofoblasto extravelloso, la evasión del sistema inmune de la madre y la remodelación de la vasculatura local. Estos y otros procesos hacen que la implantación placentaria sea notablemente similar a la invasión tumoral. Las células de cáncer deben su origen a una pérdida de la identidad celular somática acompañada del desarrollo de un fenotipo aberrante que se alcanza como consecuencia de la reactivación de programas de desarrollo embrionario. Se cree que la pérdida de la identidad celular, así como la reactivación de los programas de desarrollo se deben en gran medida a una pérdida de la estabilidad genómica, que a su vez depende del panorama de metilación a nivel genómico. Se sabe que diversos estímulos extracelulares son capaces de promover una remodelación del panorama de metilación de células somáticas tal que contribuye a una transición maligna hacia un fenotipo altamente proliferativo e invasivo, que se debe tanto a la activación estocástica de oncogenes, como a un silenciamiento de genes supresores de tumores. Utilizando la línea celular derivada de trofoblasto, HTR-8/SVneo, por su fenotipo pseudomaligno y como modelo para el estudio de la biología placentaria y de la progresión tumoral, el propósito de este trabajo fue analizar la respuesta funcional de la línea ante un estímulo mitogénico con el péptido IGF2, y determinar cómo esta respuesta se relaciona con la variación en el panorama de metilación genómico y la expresión de RNA mensajero. A través de la interrogación del panorama de metilación genómico y la actividad transcripcional de la línea celular se identificaron variaciones que aparecen por efecto del estímulo y que ocurren de manera simultánea con un aumento de la actividad proliferativa, migratoria e invasiva de la línea celular. Muchos de los genes comprometidos en estas variaciones han sido descritos previamente en cáncer y más aún, hacen parte de vías de señalización a través de las cuales podría estar procediendo el estímulo con IGF2 y que además, son importantes para la adquisición de un fenotipo maligno en diversos tipos de cáncer. En conclusión, el péptido IGF2 tiene la capacidad de aumentar la actividad proliferativa, migratoria e invasiva de la línea celular, y estos cambios ocurren de manera paralela, y probablemente como consecuencia de alteraciones a nivel epigenético y transcripcional. (Texto tomado de la fuente)The process of placental implantation depends on a thorough regulation of the invasion of the maternal decidua by the cellular subtype known as extravillous trophoblast, invasion of the maternal immune system and remodeling of the local vasculature. These and other processes make the placental implantation remarkably similar to tumor invasion. Cancer cells owe their origin to a loss of somatic cellular identity, accompanied by the development of an aberrant phenotype achieved as a consequence of the stochastic reactivation of embryonic development programs. It is believed that this loss of cellular identity, as well as the reactivation of these programs is owed to a great extent to a loss of genomic stability, which in turn depends on the DNA methylation landscape at the genomic level. It has been observed that extracellular stimuli of diverse kinds are capable of promoting a remodeling of the DNA methylation landscape in somatic cell such that it contributes to a malignant transformation to a highly proliferative, migratory and invasive phenotype, which is owed both to a stochastic activation of oncogenes and a silencing of tumor-repressor genes. Using trophoblast-derived HTR-8/SVneo cell line because its pseudo malignant phenotype and as model for the study of placental biology and tumor progression, the purpose of this work was to analyze the functional response of the cell line as a response to a mitogenic stimulus with IGF2, and to determine how this response is related to the variation of the methylation landscape and the transcriptional activity of the cell line. Through interrogation of the genomic methylation landscape and the transcriptional activity of the cell line, some alterations were identified that appear as a consequence of the stimulus and that occur simultaneously with an increase of the proliferative, migratory and invasive activity of the cell line. Many of the genes compromised in these alterations have been previously described in cancer and moreover, take part in signaling pathways through which the IGF2 stimulus could be proceeding and that are important for the acquisition of a malignant phenotype in several types of cancer. In conclusion, IGF2 peptide has the capacity of promoting the proliferative, migratory and invasive capacities of the cell line and these changes happen simultaneously, and probably as a consequence of alterations at epigenetic and transcriptional levels.MaestríaMagíster en Ciencias - BioquímicaFactores de crecimiento, diferenciación y cáncerviii, 62 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaDepartamento de QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - BioquímicaOncogenesMetilación de ADNReceptor IGF Tipo 2OncogenesDNA MethylationReceptor, IGF Type 2CáncerMetilación del DNAPlacentaFactor de crecimientoEpigenéticaCancerIGF signaling systemEpigeneticsGenomic instabilityGenic expressionMalignant transformationEfecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneoEffect of IGF2 on the DNA methylation landscape and associated mRNA expression in HTR-8/SVneo cell lineTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBurrows, T. 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Nature. 417, 949–954Ministerio de Ciencia, Tecnología e InnovaciónInvestigadoresPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/80620/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINAL1013638215.2021.pdf1013638215.2021.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf1903587https://repositorio.unal.edu.co/bitstream/unal/80620/3/1013638215.2021.pdf16497d7e774c86057593af1a23adca53MD53THUMBNAIL1013638215.2021.pdf.jpg1013638215.2021.pdf.jpgGenerated Thumbnailimage/jpeg4860https://repositorio.unal.edu.co/bitstream/unal/80620/4/1013638215.2021.pdf.jpgf655ee719b7970a7287528d030c85b70MD54unal/80620oai:repositorio.unal.edu.co:unal/806202023-07-29 23:04:00.682Repositorio Institucional Universidad Nacional de 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GVyZWNob3MgZGUgYXV0b3IgcXVlIGNvbmxsZXZlIGxhIGRpc3RyaWJ1Y2nDs24gZGUgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuCkFsIGhhY2VyIGNsaWMgZW4gZWwgc2lndWllbnRlIGJvdMOzbiwgdXN0ZWQgaW5kaWNhIHF1ZSBlc3TDoSBkZSBhY3VlcmRvIGNvbiBlc3RvcyB0w6lybWlub3MuCgpVTklWRVJTSURBRCBOQUNJT05BTCBERSBDT0xPTUJJQSAtIMOabHRpbWEgbW9kaWZpY2FjacOzbiAyNy8yMC8yMDIwCg==

Efecto de IGF2 sobre el panorama de metilación del ADN y la expresión de ARNm asociada en la línea celular HTR-8/SVneo

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