Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico

ilustraciones (principalmente a color), diagramas

Autores:: Moreno Tristancho, Angelica Natali

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico
dc.title.translated.eng.fl_str_mv	Evaluation of the interactions of polyphenolic metabolites obtained from Solanum tuberosum on the antiplatelet activity of acetylsalicylic
title	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico
spellingShingle	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico 610 - Medicina y salud::615 - Farmacología y terapéutica Solanum tuberosum Ácido clorogénico Inhibidores de agregación plaquetaria Aspirina Interacciones farmacológicas Terapia trombolítica-Efectos adversos Ácidos cafeicos Chlorogenic acid Platelet aggregation inhibitors Aspirin Drug interactions Thrombolytic therapy -Adverse effects Caffeic acids Solanum tuberosum antiagregante plaquetario sinergia ácido clorogénico ácido cafeico ácido acetilsalicílico platelet antiaggregant synergy chlorogenic acid caffeic acid acetylsalicylic acid
title_short	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico
title_full	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico
title_fullStr	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico
title_full_unstemmed	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico
title_sort	Evaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílico
dc.creator.fl_str_mv	Moreno Tristancho, Angelica Natali
dc.contributor.advisor.spa.fl_str_mv	Guerrero Pabon, Mario Francisco
dc.contributor.author.spa.fl_str_mv	Moreno Tristancho, Angelica Natali
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigaciones en Farmacología Molecular (Farmol)
dc.subject.ddc.spa.fl_str_mv	610 - Medicina y salud::615 - Farmacología y terapéutica
topic	610 - Medicina y salud::615 - Farmacología y terapéutica Solanum tuberosum Ácido clorogénico Inhibidores de agregación plaquetaria Aspirina Interacciones farmacológicas Terapia trombolítica-Efectos adversos Ácidos cafeicos Chlorogenic acid Platelet aggregation inhibitors Aspirin Drug interactions Thrombolytic therapy -Adverse effects Caffeic acids Solanum tuberosum antiagregante plaquetario sinergia ácido clorogénico ácido cafeico ácido acetilsalicílico platelet antiaggregant synergy chlorogenic acid caffeic acid acetylsalicylic acid
dc.subject.decs.spa.fl_str_mv	Solanum tuberosum Ácido clorogénico Inhibidores de agregación plaquetaria Aspirina Interacciones farmacológicas Terapia trombolítica-Efectos adversos Ácidos cafeicos
dc.subject.decs.eng.fl_str_mv	Chlorogenic acid Platelet aggregation inhibitors Aspirin Drug interactions Thrombolytic therapy -Adverse effects Caffeic acids
dc.subject.proposal.other.fl_str_mv	Solanum tuberosum
dc.subject.proposal.spa.fl_str_mv	antiagregante plaquetario sinergia ácido clorogénico ácido cafeico ácido acetilsalicílico
dc.subject.proposal.eng.fl_str_mv	platelet antiaggregant synergy chlorogenic acid caffeic acid acetylsalicylic acid
description	ilustraciones (principalmente a color), diagramas
publishDate	2023
dc.date.issued.none.fl_str_mv	2023-12
dc.date.accessioned.none.fl_str_mv	2024-01-29T19:25:16Z
dc.date.available.none.fl_str_mv	2024-01-29T19:25:16Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
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dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
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url	https://repositorio.unal.edu.co/handle/unal/85491 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
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dc.relation.references.spa.fl_str_mv	Alan D Michelson. (2013). Platelets (Alan D Michelson, Marco Cattaneo, Adrew relinger, & Peter Newman, Eds.; 3rd ed., Vol. 3) Badimon, L., & Vilahur, G. (2013). Antiagregación plaquetaria Mecanismos de acción de los diferentes agentes antiplaquetarios. In Rev Esp Cardiol Supl (Vol. 13). https://www.revespcardiol.org/?ref=1917747156 Bermejo, E. (2017). Plaquetas. Hematología, 10-18. http://www.sah.org.ar/revista/numeros/vol21/extra/06-Vol%2021-extra.pdf Buitrago, D. (2012). Estudio de los mecanismos antihipertensivos y antiagregantes plaquetarios de los metabolitos secundarios obtenidos de Solanum tuberosum. [Tesis doctoral]. Bogotá, D. C.: Universidad Nacional de Colombia. Borda,D.C. (2020). Evaluación del efecto sobre la agregación plaquetaria de una dieta enriquecida en cáscara de papa. [Tesis maestrpia]. Bogotá, D. C.: Universidad Nacional de Colombia. Buitrago, D., Puebla, P., & Guerrero, M. (2019). Antiplatelet activity of metabolites isolated from Solanum tuberosum. Latin American Journal of Pharmacy, 38(8), 1575-1581. Buitrago, D., Ramos, G., Rincón, J., & Guerrero, M. (2007). Actividad antiagregante del extracto etanólico de Solanum tuberosum en plaquetas humanas. Vitae, 14(1), 49- 54. http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121- 40042007000100007 Chen, Y., Yuan, Y., & Li, W. (2018). Sorting machineries: How platelet-dense granules differ from α-granules. In Bioscience Reports (Vol. 38, Issue 5). Portland Press Ltd. https://doi.org/10.1042/BSR20180458 Chou, T. C. (2010). Drug combination studies and their synergy quantification using the chou-talalay method. In Cancer Research (Vol. 70, Issue 2, pp. 440–446). https://doi.org/10.1158/0008-5472.CAN-09-1947 de Alencar Silva, A., Pereira-de-Morais, L., Rodrigues da Silva, R. E., de Menezes Dantas, D., Brito Milfont, C. G., Gomes, M. F., Araújo, I. M., Kerntopf, M. R., Alencar de Menezes, I. R., & Barbosa, R. (2020). Pharmacological screening of the phenolic compound caffeic acid using rat aorta, uterus and ileum smooth muscle. Chemico- Biological Interactions, 332. https://doi.org/10.1016/j.cbi.2020.109269 FAO,. (2008.). RESEÑA DE FIN DE AÑO AÑO INTERNACIONAL DE LA PAPA 2008. Roma, Italia . https://www.fao.org/3/i0500s/i0500s.pdf Di Veroli, G. Y., Fornari, C., Wang, D., Mollard, S., Bramhall, J. L., Richards, F. M., & Jodrell, D. I. (2016). Combenefit: An interactive platform for the analysis and visualization of drug combinations. Bioinformatics, 32(18), 2866–2868. https://doi.org/10.1093/bioinformatics/btw230 ENVIRONMENT DIRECTORATE JOINT MEETING OF THE CHEMICALS COMMITTEE AND THE WORKING PARTY ON CHEMICALS, PESTICIDES AND BIOTECHNOLOGY Cancels & replaces the same document of 21 December 2020 REVISED CONSENSUS DOCUMENT ON COMPOSITIONAL CONSIDERATIONS FOR NEW VARIETIES OF POTATO (Solanum tuberosum): Key Food and Feed Nutrients, Toxicants, Allergens, Anti-nutrients and Other Plant Metabolites Series on the Safety of Novel Foods and Feeds No. 33 JT03470054 OFDE. (2021) Fernando, G. L., & Frade, R. (2008). Manual de trombosis y terapia antitrombótica (V. J. Aldrete, Ed.; Vol. 1). Fuentes, E., Caballero, J., Alarcón, M., Rojas, A., & Palomo, I. (2014). Chlorogenic acid inhibits human platelet activation and thrombus formation. PLoS ONE, 9(3). https://doi.org/10.1371/journal.pone.0090699 Ghoshal, K., & Bhattacharyya, M. (2014). Overview of platelet physiology: Its hemostatic and nonhemostatic role in disease pathogenesis. In The Scientific World Journal (Vol. 2014). ScientificWorld Ltd. https://doi.org/10.1155/2014/781857 Goodman, & Gilman. (2012). Las bases farmacológicas de la terapéutica (L. Bruton, B. Chabner, & Bjorn Knollman, Eds.; 12th ed.) Gremmel, T., Frelinger, A. L., & Michelson, A. D. (2016). Platelet physiology. In Seminars in Thrombosis and Hemostasis (Vol. 42, Issue 3, pp. 191–204). Thieme Medical Publishers, Inc. https://doi.org/10.1055/s-0035-1564835 Guadalupe Sánchez-Arias, A., Bobadilla-Serrano, M. E., Dimas-Altamirano, B., Gómez- Ortega, M., & González-González, G. (n.d.). Enfermedad cardiovascular: primera causa de morbilidad en un hospital de tercer nivel Heart diseases: the leading cause of morbidity in a third-level hospital. www.medigraphic.com/revmexcardiolwww.medigraphic.org.mx Jm, C. (2017.). Fisiología de la hemostasia. Introducción general Normal haemostasis. Introduction. (Vol. 21. 4–6). https://www.sah.org.ar/revistasah/numeros/vol21/extra/04-Vol%2021-extra.pdf Li, Y., Shi, W., Li, Y., Zhou, Y., Hu, X., Song, C., Ma, H., Wang, C., & Li, Y. (2008). Neuroprotective effects of chlorogenic acid against apoptosis of PC12 cells induced by methylmercury. Environmental Toxicology and Pharmacology, 26(1), 13–21. https://doi.org/10.1016/j.etap.2007.12.008 Harrison, P. (2005). Platelet function analysis. Blood Reviews, 19(2), 111–123. https://doi.org/10.1016/j.blre.2004.05.002 Lim, T. K., & Lim, T. K. (2016). Solanum tuberosum. Edible Medicinal and Non-Medicinal Plants, 12–93. https://doi.org/10.1007/978-3-319-26065-5_2 Linden, M., Frelinger, A., Barnard, M., Przyklenk, K., Furman, M., & Michelson, A. (2004). Application of flow cytometry to platelet disorders. Seminars in Thrombosis and Hemostasis, 30(5), 501-511. https://doi.org/10.1055/s-2004-835671 Machlus, K. R., & Italiano, J. E. (2013). The incredible journey: From megakaryocyte development to platelet formation. In Journal of Cell Biology (Vol. 201, Issue 6, pp. 785–796). https://doi.org/10.1083/jcb.201304054 Linden, M., Frelinger, A., Barnard, M., Przyklenk, K., Furman, M., & Michelson, A. (2004). Application of flow cytometry to platelet disorders. Seminars in Thrombosis and Hemostasis, 30(5), 501-511. https://doi.org/10.1055/s-2004-835671 Machlus, K. R., & Italiano, J. E. (2013). The incredible journey: From megakaryocyte development to platelet formation. In Journal of Cell Biology (Vol. 201, Issue 6, pp. 785–796). https://doi.org/10.1083/jcb.201304054 Ministerio de Salud. (1993). Resolución 8430 del 4 de octubre de 1993. [Por la cual se establecen las normas científicas, técnicas y administrativas para la investigación en salud]. Bogotá, D. C., Colombia. https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/DE/DIJ/RESOLUCI ON-8430-DE-1993.PDF Miao, M., & Xiang, L. (2020). Pharmacological action and potential targets of chlorogenic acid. In Advances in Pharmacology (Vol. 87, pp. 71–88). Academic Press Inc. https://doi.org/10.1016/bs.apha.2019.12.002 Park, J. B. (2015). Potential Effects of Chlorogenic Acids on Platelet Activation. In Coffee in Health and Disease Prevention (pp. 709–717). Elsevier Inc. https://doi.org/10.1016/B978-0-12-409517-5.00079-6 Pavlíková, N. (2023). Caffeic Acid and Diseases—Mechanisms of Action. In International Journal of Molecular Sciences (Vol. 24, Issue 1). MDPI. https://doi.org/10.3390/ijms24010588 Rubenstein, D. A., & Yin, W. (2018). Platelet-activation mechanisms and vascular remodeling. Comprehensive Physiology, 8(3), 1117–1156. https://doi.org/10.1002/cphy.c170049 Solla, I., Bembibre, L., & Freire, J. (2011). Manejo del Síndrome coronario agudo en Urgencias de Atención Primaria. Cadernos de Atención Primaria, 18(1), 49-55. https://www.agamfec.com/wp/wp-content/uploads/2014/07/18_1_actua_1.pdf Tang, J., Wennerberg, K., & Aittokallio, T. (2015). What is synergy? The Saariselkï¿½ agreement revisited. In Frontiers in Pharmacology (Vol. 6, Issue SEP). Frontiers Media S.A. https://doi.org/10.3389/fphar.2015.00181 Tom, E. N. L., Girard-Thernier, C., & Demougeot, C. (2016). The Janus face of chlorogenic acid on vascular reactivity: A study on rat isolated vessels. Phytomedicine, 23(10), 1037–1042. https://doi.org/10.1016/j.phymed.2016.06.012 Tyszka-Czochara, M., Bukowska-Strakova, K., Kocemba-Pilarczyk, K. A., & Majka, M. (2018). Caffeic acid targets AMPK signaling and regulates tricarboxylic acid cycle anaplerosis while metformin downregulates HIF-1α-induced glycolytic enzymes in human cervical squamous cell carcinoma lines. Nutrients, 10(7). https://doi.org/10.3390/nu10070841 Ulrich-Merzenich, G. S. (2014). Combination screening of synthetic drugs and plant derived natural products-Potential and challenges for drug development. In Synergy (Vol. 1, Issue 1, pp. 59–69). Elsevier GmbH. https://doi.org/10.1016/j.synres.2014.07.011 Van der Meijden, P. E. J., & Heemskerk, J. W. M. (2019). Platelet biology and functions: new concepts and clinical perspectives. In Nature Reviews Cardiology (Vol. 16, Issue 3, pp. 166–179). Nature Publishing Group. https://doi.org/10.1038/s41569-018-0110- 0 Van der Meijden, P. E. J., & Heemskerk, J. W. M. (2019). Platelet biology and functions: new concepts and clinical perspectives. In Nature Reviews Cardiology (Vol. 16, Issue 3, pp. 166–179). Nature Publishing Group. https://doi.org/10.1038/s41569-018-0110- 0 Yang, Y., Zhang, Z., Li, S., Ye, X., Li, X., & He, K. (2014). Synergy effects of herb extracts: Pharmacokinetics and pharmacodynamic basis. In Fitoterapia (Vol. 92, pp. 133–147). https://doi.org/10.1016/j.fitote.2013.10.010 Yeung, J., Li, W., & Holinstat, M. (2018). Platelet signaling and disease: Targeted therapy for thrombosis and other related diseases. Pharmacological Reviews, 70(3), 526–548. https://doi.org/10.1124/pr.117.014530
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Maestría en Ciencias - Farmacología
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Guerrero Pabon, Mario Franciscodebd7ef8173a9e9f01b1d9ee0719c26aMoreno Tristancho, Angelica Natali6230e2e942b79184234a2ec3b884cb36Grupo de Investigaciones en Farmacología Molecular (Farmol)2024-01-29T19:25:16Z2024-01-29T19:25:16Z2023-12https://repositorio.unal.edu.co/handle/unal/85491Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones (principalmente a color), diagramasLa combinación de terapias es una estrategia efectiva en el ámbito clínico, ya que mejora la respuesta farmacológica y reduce el riesgo de efectos secundarios. Se investigó el efecto antiagregante de los polifenoles ácido cafeico (AC) y ácido clorogénico (ACG) de Solanum tuberosum, junto con ácido acetilsalicílico (ASA), en plasma rico en plaquetas. Los resultados mostraron efectos dependientes de la concentración de AC y ACG, así como de ASA. Se observó una interacción sinérgica con AC y una disminución en la efectividad antiagregante con ACG. Se necesitan estudios adicionales para determinar las concentraciones óptimas y posibles efectos sinérgicos de estos compuestos combinados con ASA. La combinación de terapias es una estrategia efectiva en el ámbito clínico, ya que mejora la respuesta farmacológica y reduce el riesgo de efectos secundarios. Se investigó el efecto antiagregante de los polifenoles ácido cafeico (AC) y ácido clorogénico (ACG) de Solanum tuberosum, junto con ácido acetilsalicílico (ASA), en plasma rico en plaquetas. Los resultados mostraron efectos dependientes de la concentración de AC y ACG, así como de ASA. Se observó una interacción sinérgica con AC y una disminución en la efectividad antiagregante con ACG. Se necesitan estudios adicionales para determinar las concentraciones óptimas y posibles efectos sinérgicos de estos compuestos combinados con ASA. (Texto tomado de la fuente)Combination therapy is a therapeutic strategy that can be highly effective in the clinical setting, since drugs used in association can improve the pharmacological response and, at the same time, make it possible to reduce doses and the risk of possible side effects (Yang et al., 2014). Considering this approach, it is appropriate to investigate or explore new therapeutic options that contribute to decrease the impact of thrombotic diseases on public health. Therapies based on natural sources could offer active metabolites that, when interacting synergistically, provide new possibilities for combination therapies at the pharmacological level. In this study, the antiaggregation effect of the polyphenols previously identified in Solanum tuberosum, caffeic acid and chlorogenic acid, in the absence and in combination with increasing concentrations of acetylsalicylic acid (ASA), was analyzed against the agonist arachidonic acid (AA), in platelet-rich plasma from healthy volunteers, using the Born spectrophotometric technique, which is based on the kinetics of platelet aggregation analyzed by turbidimetry (Born, 1962). The aggregometer allowed observing the changes in light transmission in the blood plasma after incubation with the test reagents and the platelet aggregation-inducing agent arachidonic acid (AA), so that the increase in the percentage of platelet aggregation was evident by the increase in light transmission through the cell, due to the platelets aggregating and settling at the bottom of the vessel. Considering that caffeic acid and chlorogenic acid are active metabolites with antiplatelet aggregation activity present in Solanum tuberosum, in this work we proposed to study the possible interactions that they exert when combined with the reference drug, acetylsalicylic acid, one of the most widely used agents in clinical practice. The objective was to determine whether they could exert coadjuvant effects as antiaggregants, which could eventually be useful in the therapy or prevention of atherothrombotic disorders. The results, under the experimental conditions set in this work, showed concentration- dependent effects of caffeic acid and chlorogenic acid, with effective concentrations 50 (IC50) of 1x10-3 and 3,4x10-4 M, respectively, while with ASA, in the range of concentrations tested, an antiaggregant effect was observed that decreases with increasing concentration (from 5,5x10-5 to 1,6x10-3M), which is consistent with its mechanism of action, antiaggregant at low concentrations and proaggregant at higher concentrations. When examining the interaction of caffeic acid (in the concentration range 1.5 - 3.0x10-4 M) with ASA (5.5x10-6M), an increase in the antiaggregation effect was observed that decreased with concentration, while the interaction of chlorogenic acid (in the range 2.26x10-4 - 5.6x10-3 M) with ASA (5.5x10-6M) did not show an increase in the antiaggregation effect, but rather a dose-dependent decrease. In conclusion, concentration-dependent antiaggregation effects are confirmed with the polyphenolic compounds caffeic acid and chlorogenic acid, a dose-dependent decreasing antiaggregation effect of ASA, and an interaction suggesting synergistic effect with caffeic acid; however, the sample size and concentration range need to be expanded to identify the appropriate range of concentrations of these compounds that exert possible synergistic effects when combined with ASA.MaestríaMagíster en Ciencias - FarmacologíaFarmacología experimental Cardiovascular80 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - FarmacologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::615 - Farmacología y terapéuticaSolanum tuberosumÁcido clorogénicoInhibidores de agregación plaquetariaAspirinaInteracciones farmacológicasTerapia trombolítica-Efectos adversosÁcidos cafeicosChlorogenic acidPlatelet aggregation inhibitorsAspirinDrug interactionsThrombolytic therapy -Adverse effectsCaffeic acidsSolanum tuberosumantiagregante plaquetariosinergiaácido clorogénicoácido cafeicoácido acetilsalicílicoplatelet antiaggregantsynergychlorogenic acidcaffeic acidacetylsalicylic acidEvaluación de las interacciones de metabolitos polifenólicos obtenidos de Solanum tuberosum sobre la actividad antiagregante plaquetario del ácido acetil salicílicoEvaluation of the interactions of polyphenolic metabolites obtained from Solanum tuberosum on the antiplatelet activity of acetylsalicylicTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlan D Michelson. (2013). Platelets (Alan D Michelson, Marco Cattaneo, Adrew relinger, & Peter Newman, Eds.; 3rd ed., Vol. 3)Badimon, L., & Vilahur, G. (2013). Antiagregación plaquetaria Mecanismos de acción de los diferentes agentes antiplaquetarios. In Rev Esp Cardiol Supl (Vol. 13). https://www.revespcardiol.org/?ref=1917747156Bermejo, E. (2017). Plaquetas. Hematología, 10-18. http://www.sah.org.ar/revista/numeros/vol21/extra/06-Vol%2021-extra.pdfBuitrago, D. (2012). Estudio de los mecanismos antihipertensivos y antiagregantes plaquetarios de los metabolitos secundarios obtenidos de Solanum tuberosum. [Tesis doctoral]. Bogotá, D. C.: Universidad Nacional de Colombia. Borda,D.C. (2020). Evaluación del efecto sobre la agregación plaquetaria de una dieta enriquecida en cáscara de papa. [Tesis maestrpia]. Bogotá, D. C.: Universidad Nacional de Colombia.Buitrago, D., Puebla, P., & Guerrero, M. (2019). Antiplatelet activity of metabolites isolated from Solanum tuberosum. Latin American Journal of Pharmacy, 38(8), 1575-1581.Buitrago, D., Ramos, G., Rincón, J., & Guerrero, M. (2007). Actividad antiagregante del extracto etanólico de Solanum tuberosum en plaquetas humanas. Vitae, 14(1), 49- 54. http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121- 40042007000100007Chen, Y., Yuan, Y., & Li, W. (2018). Sorting machineries: How platelet-dense granules differ from α-granules. In Bioscience Reports (Vol. 38, Issue 5). Portland Press Ltd. https://doi.org/10.1042/BSR20180458Chou, T. C. (2010). Drug combination studies and their synergy quantification using the chou-talalay method. In Cancer Research (Vol. 70, Issue 2, pp. 440–446). https://doi.org/10.1158/0008-5472.CAN-09-1947de Alencar Silva, A., Pereira-de-Morais, L., Rodrigues da Silva, R. E., de Menezes Dantas, D., Brito Milfont, C. G., Gomes, M. F., Araújo, I. M., Kerntopf, M. R., Alencar de Menezes, I. R., & Barbosa, R. (2020). 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Pharmacological Reviews, 70(3), 526–548. https://doi.org/10.1124/pr.117.014530EstudiantesInvestigadoresMaestrosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85491/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1053586975.2023.pdf1053586975.2023.pdfTesis de Maestría en Ciencias Farmacologíaapplication/pdf1952851https://repositorio.unal.edu.co/bitstream/unal/85491/2/1053586975.2023.pdf97be65246a0b3ac9fa58c74b7dd5689fMD52THUMBNAIL1053586975.2023.pdf.jpg1053586975.2023.pdf.jpgGenerated Thumbnailimage/jpeg4773https://repositorio.unal.edu.co/bitstream/unal/85491/3/1053586975.2023.pdf.jpgfc0b98a4d226b717241e6a59fb6cb245MD53unal/85491oai:repositorio.unal.edu.co:unal/854912024-01-29 23:03:52.037Repositorio Institucional Universidad Nacional de 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