Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability...
- Autores:
-
Henao Uribe, Francisco Javier
Valencia Giraldo, Julian
Mesa, Henry
Gómez Londoño, Germán
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/58565
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/58565
http://bdigital.unal.edu.co/55349/
- Palabra clave:
- 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
Boar
Reproduction
Seminal preservation
Seminal quality
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Henao Uribe, Francisco Javier8347b7ae-dbbe-4824-9999-25f91518b40c300Valencia Giraldo, Julian98cabab5-8581-4b18-ba02-968ba53fb96c300Mesa, Henry09b9052a-3e7b-4e83-932e-426ca032e98f300Gómez Londoño, Germán7319c36e-40f5-4138-a477-c7ec7de0c5593002019-07-02T14:22:55Z2019-07-02T14:22:55Z2016-07-01ISSN: 2248-7026https://repositorio.unal.edu.co/handle/unal/58565http://bdigital.unal.edu.co/55349/The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability (MHF) and two with low freezability (MLF), according to the percentage of functionally competent sperm (FCS). Immediately after the collection was completed, the SP and sperm from the males selected were separated by centrifugation to combine the two types of plasma with the two types of sperm, incubate them for three hours and then freeze them. The variables evaluated were: sperm morphology, structural and functional integrity of plasmatic membrane, progressive and total motility, DNA fragmentation, acrosome integrity, capacitated sperm and FCS. The combination of sperm and plasma of MHF recorded the highest value (P0.01) of acrosome integrity (24.3 ± 0.082 vs 6.076 ± 0.16) when compared to MLF plasma and cells. Membrane structural integrity was higher (P0.01) with MHF (53.56 ± 0.0395) than with MLF plasma (47.49 ± 0.0419). The differences in porcine semen freezability depend on interactions between seminal plasma and sperm.application/pdfspaUniversidad Nacional de Colombiahttp://www.revistas.unal.edu.co/index.php/refame/article/view/59135Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía MedellínRevista Facultad Nacional de Agronomía MedellínHenao Uribe, Francisco Javier and Valencia Giraldo, Julian and Mesa, Henry and Gómez Londoño, Germán (2016) Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation. Revista Facultad Nacional de Agronomía, 69 (2). pp. 7903-7910. ISSN 2248-702657 Ciencias de la vida; Biología / Life sciences; biology58 Plantas / PlantsBoarReproductionSeminal preservationSeminal qualityEffect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservationArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINALrfna.v69n2.59135.pdfapplication/pdf279671https://repositorio.unal.edu.co/bitstream/unal/58565/1/rfna.v69n2.59135.pdfed9d4c0f705cfa4f0a5f8a1c1de5e165MD51THUMBNAILrfna.v69n2.59135.pdf.jpgrfna.v69n2.59135.pdf.jpgGenerated Thumbnailimage/jpeg7766https://repositorio.unal.edu.co/bitstream/unal/58565/2/rfna.v69n2.59135.pdf.jpgd33d5b376b6ab257f5b1f32389f6f401MD52unal/58565oai:repositorio.unal.edu.co:unal/585652024-04-02 23:11:23.473Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation |
title |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation |
spellingShingle |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation 57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants Boar Reproduction Seminal preservation Seminal quality |
title_short |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation |
title_full |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation |
title_fullStr |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation |
title_full_unstemmed |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation |
title_sort |
Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation |
dc.creator.fl_str_mv |
Henao Uribe, Francisco Javier Valencia Giraldo, Julian Mesa, Henry Gómez Londoño, Germán |
dc.contributor.author.spa.fl_str_mv |
Henao Uribe, Francisco Javier Valencia Giraldo, Julian Mesa, Henry Gómez Londoño, Germán |
dc.subject.ddc.spa.fl_str_mv |
57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants |
topic |
57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants Boar Reproduction Seminal preservation Seminal quality |
dc.subject.proposal.spa.fl_str_mv |
Boar Reproduction Seminal preservation Seminal quality |
description |
The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability (MHF) and two with low freezability (MLF), according to the percentage of functionally competent sperm (FCS). Immediately after the collection was completed, the SP and sperm from the males selected were separated by centrifugation to combine the two types of plasma with the two types of sperm, incubate them for three hours and then freeze them. The variables evaluated were: sperm morphology, structural and functional integrity of plasmatic membrane, progressive and total motility, DNA fragmentation, acrosome integrity, capacitated sperm and FCS. The combination of sperm and plasma of MHF recorded the highest value (P0.01) of acrosome integrity (24.3 ± 0.082 vs 6.076 ± 0.16) when compared to MLF plasma and cells. Membrane structural integrity was higher (P0.01) with MHF (53.56 ± 0.0395) than with MLF plasma (47.49 ± 0.0419). The differences in porcine semen freezability depend on interactions between seminal plasma and sperm. |
publishDate |
2016 |
dc.date.issued.spa.fl_str_mv |
2016-07-01 |
dc.date.accessioned.spa.fl_str_mv |
2019-07-02T14:22:55Z |
dc.date.available.spa.fl_str_mv |
2019-07-02T14:22:55Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.issn.spa.fl_str_mv |
ISSN: 2248-7026 |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/58565 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/55349/ |
identifier_str_mv |
ISSN: 2248-7026 |
url |
https://repositorio.unal.edu.co/handle/unal/58565 http://bdigital.unal.edu.co/55349/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://www.revistas.unal.edu.co/index.php/refame/article/view/59135 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía Medellín Revista Facultad Nacional de Agronomía Medellín |
dc.relation.references.spa.fl_str_mv |
Henao Uribe, Francisco Javier and Valencia Giraldo, Julian and Mesa, Henry and Gómez Londoño, Germán (2016) Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation. Revista Facultad Nacional de Agronomía, 69 (2). pp. 7903-7910. ISSN 2248-7026 |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Universidad Nacional de Colombia |
institution |
Universidad Nacional de Colombia |
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