Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation

The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability...

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Autores:
Henao Uribe, Francisco Javier
Valencia Giraldo, Julian
Mesa, Henry
Gómez Londoño, Germán
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/58565
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/58565
http://bdigital.unal.edu.co/55349/
Palabra clave:
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
Boar
Reproduction
Seminal preservation
Seminal quality
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Henao Uribe, Francisco Javier8347b7ae-dbbe-4824-9999-25f91518b40c300Valencia Giraldo, Julian98cabab5-8581-4b18-ba02-968ba53fb96c300Mesa, Henry09b9052a-3e7b-4e83-932e-426ca032e98f300Gómez Londoño, Germán7319c36e-40f5-4138-a477-c7ec7de0c5593002019-07-02T14:22:55Z2019-07-02T14:22:55Z2016-07-01ISSN: 2248-7026https://repositorio.unal.edu.co/handle/unal/58565http://bdigital.unal.edu.co/55349/The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability (MHF) and two with low freezability (MLF), according to the percentage of functionally competent sperm (FCS). Immediately after the collection was completed, the SP and sperm from the males selected were separated by centrifugation to combine the two types of plasma with the two types of sperm, incubate them for three hours and then freeze them. The variables evaluated were: sperm morphology, structural and functional integrity of plasmatic membrane, progressive and total motility, DNA fragmentation, acrosome integrity, capacitated sperm and FCS. The combination of sperm and plasma of MHF recorded the highest value (P0.01) of acrosome integrity (24.3 ± 0.082 vs 6.076 ± 0.16) when compared to MLF plasma and cells. Membrane structural integrity was higher (P0.01) with MHF (53.56 ± 0.0395) than with MLF plasma (47.49 ± 0.0419). The differences in porcine semen freezability depend on interactions between seminal plasma and sperm.application/pdfspaUniversidad Nacional de Colombiahttp://www.revistas.unal.edu.co/index.php/refame/article/view/59135Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía MedellínRevista Facultad Nacional de Agronomía MedellínHenao Uribe, Francisco Javier and Valencia Giraldo, Julian and Mesa, Henry and Gómez Londoño, Germán (2016) Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation. Revista Facultad Nacional de Agronomía, 69 (2). pp. 7903-7910. ISSN 2248-702657 Ciencias de la vida; Biología / Life sciences; biology58 Plantas / PlantsBoarReproductionSeminal preservationSeminal qualityEffect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservationArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINALrfna.v69n2.59135.pdfapplication/pdf279671https://repositorio.unal.edu.co/bitstream/unal/58565/1/rfna.v69n2.59135.pdfed9d4c0f705cfa4f0a5f8a1c1de5e165MD51THUMBNAILrfna.v69n2.59135.pdf.jpgrfna.v69n2.59135.pdf.jpgGenerated Thumbnailimage/jpeg7766https://repositorio.unal.edu.co/bitstream/unal/58565/2/rfna.v69n2.59135.pdf.jpgd33d5b376b6ab257f5b1f32389f6f401MD52unal/58565oai:repositorio.unal.edu.co:unal/585652024-04-02 23:11:23.473Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
title Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
spellingShingle Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
Boar
Reproduction
Seminal preservation
Seminal quality
title_short Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
title_full Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
title_fullStr Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
title_full_unstemmed Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
title_sort Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
dc.creator.fl_str_mv Henao Uribe, Francisco Javier
Valencia Giraldo, Julian
Mesa, Henry
Gómez Londoño, Germán
dc.contributor.author.spa.fl_str_mv Henao Uribe, Francisco Javier
Valencia Giraldo, Julian
Mesa, Henry
Gómez Londoño, Germán
dc.subject.ddc.spa.fl_str_mv 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
topic 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
Boar
Reproduction
Seminal preservation
Seminal quality
dc.subject.proposal.spa.fl_str_mv Boar
Reproduction
Seminal preservation
Seminal quality
description The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability (MHF) and two with low freezability (MLF), according to the percentage of functionally competent sperm (FCS). Immediately after the collection was completed, the SP and sperm from the males selected were separated by centrifugation to combine the two types of plasma with the two types of sperm, incubate them for three hours and then freeze them. The variables evaluated were: sperm morphology, structural and functional integrity of plasmatic membrane, progressive and total motility, DNA fragmentation, acrosome integrity, capacitated sperm and FCS. The combination of sperm and plasma of MHF recorded the highest value (P0.01) of acrosome integrity (24.3 ± 0.082 vs 6.076 ± 0.16) when compared to MLF plasma and cells. Membrane structural integrity was higher (P0.01) with MHF (53.56 ± 0.0395) than with MLF plasma (47.49 ± 0.0419). The differences in porcine semen freezability depend on interactions between seminal plasma and sperm.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-07-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T14:22:55Z
dc.date.available.spa.fl_str_mv 2019-07-02T14:22:55Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 2248-7026
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/58565
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/55349/
identifier_str_mv ISSN: 2248-7026
url https://repositorio.unal.edu.co/handle/unal/58565
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dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://www.revistas.unal.edu.co/index.php/refame/article/view/59135
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Facultad Nacional de Agronomía Medellín
Revista Facultad Nacional de Agronomía Medellín
dc.relation.references.spa.fl_str_mv Henao Uribe, Francisco Javier and Valencia Giraldo, Julian and Mesa, Henry and Gómez Londoño, Germán (2016) Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation. Revista Facultad Nacional de Agronomía, 69 (2). pp. 7903-7910. ISSN 2248-7026
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
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