Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum
ilustraciones (algunas a color), diagramas, fotografías
- Autores:
-
García Suárez, Angélica Tatiana
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/85527
- Palabra clave:
- 540 - Química y ciencias afines::546 - Química inorgánica
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Metal nanoparticles
Nanopartículas metálicas
Ralstonia solanacearum
Bactericidas
Bactericides
Marchitez bacteriana del plátano
Enfermedades bacterianas de las plantas
Bacterias fitopatógenas
Bacterial blight (Plantain banana)
Bacterial diseases of plants
Bacteria, Phytopathogenic
Nanopartículas
Agente antibacteriano
Marchitez bacteriana
Ralstonia solanacearum
Fitopatógeno
Nanoparticles
Antibacterial agent
Bacterial wilt
Phytopathogen
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum |
| dc.title.translated.eng.fl_str_mv |
Synthesis, characterization and evaluation of the antibacterial activity of copper and silver nanoparticles against the phytopathogen Ralstonia solanacearum |
| title |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum |
| spellingShingle |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum 540 - Química y ciencias afines::546 - Química inorgánica 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Metal nanoparticles Nanopartículas metálicas Ralstonia solanacearum Bactericidas Bactericides Marchitez bacteriana del plátano Enfermedades bacterianas de las plantas Bacterias fitopatógenas Bacterial blight (Plantain banana) Bacterial diseases of plants Bacteria, Phytopathogenic Nanopartículas Agente antibacteriano Marchitez bacteriana Ralstonia solanacearum Fitopatógeno Nanoparticles Antibacterial agent Bacterial wilt Phytopathogen |
| title_short |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum |
| title_full |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum |
| title_fullStr |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum |
| title_full_unstemmed |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum |
| title_sort |
Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum |
| dc.creator.fl_str_mv |
García Suárez, Angélica Tatiana |
| dc.contributor.advisor.spa.fl_str_mv |
González Almario, Adriana Duarte Ruiz, Álvaro |
| dc.contributor.author.spa.fl_str_mv |
García Suárez, Angélica Tatiana |
| dc.contributor.researchgroup.spa.fl_str_mv |
Nuevos Materiales Nano y Supramoleculares |
| dc.subject.ddc.spa.fl_str_mv |
540 - Química y ciencias afines::546 - Química inorgánica 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales |
| topic |
540 - Química y ciencias afines::546 - Química inorgánica 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales Metal nanoparticles Nanopartículas metálicas Ralstonia solanacearum Bactericidas Bactericides Marchitez bacteriana del plátano Enfermedades bacterianas de las plantas Bacterias fitopatógenas Bacterial blight (Plantain banana) Bacterial diseases of plants Bacteria, Phytopathogenic Nanopartículas Agente antibacteriano Marchitez bacteriana Ralstonia solanacearum Fitopatógeno Nanoparticles Antibacterial agent Bacterial wilt Phytopathogen |
| dc.subject.lcc.eng.fl_str_mv |
Metal nanoparticles |
| dc.subject.lcc.spa.fl_str_mv |
Nanopartículas metálicas |
| dc.subject.agrovoc.spa.fl_str_mv |
Ralstonia solanacearum Bactericidas |
| dc.subject.agrovoc.eng.fl_str_mv |
Bactericides |
| dc.subject.lemb.spa.fl_str_mv |
Marchitez bacteriana del plátano Enfermedades bacterianas de las plantas Bacterias fitopatógenas |
| dc.subject.lemb.eng.fl_str_mv |
Bacterial blight (Plantain banana) Bacterial diseases of plants Bacteria, Phytopathogenic |
| dc.subject.proposal.spa.fl_str_mv |
Nanopartículas Agente antibacteriano Marchitez bacteriana Ralstonia solanacearum Fitopatógeno |
| dc.subject.proposal.eng.fl_str_mv |
Nanoparticles Antibacterial agent Bacterial wilt Phytopathogen |
| description |
ilustraciones (algunas a color), diagramas, fotografías |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023-11-17 |
| dc.date.accessioned.none.fl_str_mv |
2024-01-30T16:44:43Z |
| dc.date.available.none.fl_str_mv |
2024-01-30T16:44:43Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/85527 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
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https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/85527 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2González Almario, Adriana830a04311a4cbfb2ec2c69bc62a73252Duarte Ruiz, Álvaro784d4857833b3916559551e9f6d84631600García Suárez, Angélica Tatianaa8d09ce3a7538c9041fccfde156232dbNuevos Materiales Nano y Supramoleculares2024-01-30T16:44:43Z2024-01-30T16:44:43Z2023-11-17https://repositorio.unal.edu.co/handle/unal/85527Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones (algunas a color), diagramas, fotografíasRalstonia solanacearum es una bacteria fitopatógena que afecta a un amplio rango de hospedantes de importancia agronómica entre los que se encuentra el plátano (Musa x paradisiaca), causando la enfermedad denominada Moko o Maduraviche que afecta un amplio rango de musáceas a nivel mundial, siendo este un producto hortícola de importancia en la alimentación humana y en la economía colombiana. Para el control de esta enfermedad se han utilizado diferentes pesticidas y antibióticos, pero la aplicación de estos productos no muestra resultados satisfactorios. En este estudio se sintetizaron dos tamaños diferentes de nanopartículas de plata [1-AgNPs] y [2-AgNPs] mediante reducción química empleando nitrato de plata, borohidruro de sodio y citrato de sodio, y para la síntesis de nanopartículas de cobre [1-CuNPs] y [2-CuNPs], cloruro de cobre y ácido L-ascórbico. Para la caracterización se emplearon técnicas como: Dispersión Dinámica de Luz (DLS), Espectroscopia Ultravioleta Visible (Uv-Vis), Espectroscopia Infrarroja (IR), Espectroscopia Raman, Difracción de Rayos X (DRX), Absorción Atómica de Llama (FAAS), Microscopia Electrónica de Barrido (SEM) y Microscopia Electrónica de Transmisión del Alta Resolución (HRTEM), con el fin de identificar la composición, distribución de tamaño y morfología. Posteriormente, a partir de un pseudotallo de plátano proveniente de un cultivo de Norte de Santander - Colombia, que presentaba síntomas de necrosis de haces vasculares y exudado bacteriano, se aisló e identificó R. solanacearum y se evaluó in vitro la actividad antibacteriana de las nanopartículas contra este fitopatógeno por el método de difusión en pozo y se determinó la concentración mínima inhibitoria (CMI). Como resultado se evidenció una mayor actividad antibacteriana para las nanopartículas de cobre demostrando su potencial para abordar los desafíos en el tratamiento de esta enfermedad. (Texto tomado de la fuente)Ralstonia solanacearum is a phytopathogenic bacterium that affects a wide range of hosts of agronomic importance, among which is the plantain (Musa x paradisiaca), causing the disease called Moko or Maduraviche that affects a wide range of musaceae worldwide, this being an important horticultural product in human nutrition and in the Colombian economy. For the control of this disease, different pesticides and antibiotics have been used, but the application of these products not show satisfactory results. Therefore, in this study, two different sizes of silver nanoparticles [1-AgNPs] and [2-AgNPs] were synthesized by chemical reduction using silver nitrate, sodium borohydride and sodium citrate, and for the synthesis of copper nanoparticles [1- CuNPs] and [2-CuNPs], copper chloride and L-ascorbic acid. For the characterization, techniques such as: Dynamic Light Scattering (DLS), Ultraviolet Visible Spectroscopy (Uv-Vis), Infrared Spectroscopy (IR), Raman Spectroscopy, X-ray Diffraction (XRD), Flame Atomic Absorption (FAAS), were used. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), in order to identify the composition, size distribution and morphology. Subsequently, from a plantain pseudostem from a crop in Norte de Santander - Colombia, which presented symptoms of necrosis of vascular bundles and bacterial exudate, R. solanacearum was isolated and identified and evaluated in vitro the antibacterial activity of the nanoparticles against this phytopathogen by the well diffusion method and the minimum inhibitory concentration (MIC) was determined. As a result, a greater antibacterial activity was evidenced for copper nanoparticles, demonstrating their potential to address the challenges in the treatment of this disease.MaestríaMagíster en Ciencias- Químicaxviii, 133 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::546 - Química inorgánica630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesMetal nanoparticlesNanopartículas metálicasRalstonia solanacearumBactericidasBactericidesMarchitez bacteriana del plátanoEnfermedades bacterianas de las plantasBacterias fitopatógenasBacterial blight (Plantain banana)Bacterial diseases of plantsBacteria, PhytopathogenicNanopartículasAgente antibacterianoMarchitez bacterianaRalstonia solanacearumFitopatógenoNanoparticlesAntibacterial agentBacterial wiltPhytopathogenSíntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearumSynthesis, characterization and evaluation of the antibacterial activity of copper and silver nanoparticles against the phytopathogen Ralstonia solanacearumTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMPaudel, S.; Dobhal, S.; Alvarez, A. 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Microbiol. 2016, 1–10Proyecto aprobado No. 45667, registrado en HERMESUniversidad Nacional de Colombia - Resolución de la UGI Facultad de Ciencias - Sede BogotáBibliotecariosConsejerosEstudiantesInvestigadoresMaestrosMedios de comunicaciónPersonal de apoyo escolarPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85527/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1022349067.2023.pdf1022349067.2023.pdfMaestría en Ciencias - Químicaapplication/pdf6407701https://repositorio.unal.edu.co/bitstream/unal/85527/2/1022349067.2023.pdf5feedae4a6596fb6c480ab2beb46fc97MD52THUMBNAIL1022349067.2023.pdf.jpg1022349067.2023.pdf.jpgGenerated Thumbnailimage/jpeg4386https://repositorio.unal.edu.co/bitstream/unal/85527/3/1022349067.2023.pdf.jpg491ba3f5030d3276c9779a6255affda7MD53unal/85527oai:repositorio.unal.edu.co:unal/855272024-08-22 23:10:09.129Repositorio Institucional Universidad Nacional de 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