Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida
ilustraciones, diagramas, fotografías, tablas
- Autores:
-
Ramírez Perdomo, Gabriel Andrés
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/82893
- Palabra clave:
- 540 - Química y ciencias afines::547 - Química orgánica
Polimerización
Polymerization
Resorcinarenos
Sistemas huésped–hospedero
Interacciones moleculares
L-carnitina
Cuantificación
Resorcinarenes
Host-guest systems
Molecular interactions
L-carnitine
Quantification
Química de los polímeros
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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|
| dc.title.spa.fl_str_mv |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida |
| dc.title.translated.eng.fl_str_mv |
Obtaining a methacrylate-based polymeric surface modified with resorcinarenes and evaluation of its application in the preconcentration of carnitine by the solid phase extraction technique |
| title |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida |
| spellingShingle |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida 540 - Química y ciencias afines::547 - Química orgánica Polimerización Polymerization Resorcinarenos Sistemas huésped–hospedero Interacciones moleculares L-carnitina Cuantificación Resorcinarenes Host-guest systems Molecular interactions L-carnitine Quantification Química de los polímeros |
| title_short |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida |
| title_full |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida |
| title_fullStr |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida |
| title_full_unstemmed |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida |
| title_sort |
Obtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólida |
| dc.creator.fl_str_mv |
Ramírez Perdomo, Gabriel Andrés |
| dc.contributor.advisor.none.fl_str_mv |
Maldonado Villamil, Mauricio |
| dc.contributor.author.none.fl_str_mv |
Ramírez Perdomo, Gabriel Andrés |
| dc.contributor.researchgroup.spa.fl_str_mv |
Aplicaciones Analíticas de Compuestos Orgánicos (Aaco) |
| dc.subject.ddc.spa.fl_str_mv |
540 - Química y ciencias afines::547 - Química orgánica |
| topic |
540 - Química y ciencias afines::547 - Química orgánica Polimerización Polymerization Resorcinarenos Sistemas huésped–hospedero Interacciones moleculares L-carnitina Cuantificación Resorcinarenes Host-guest systems Molecular interactions L-carnitine Quantification Química de los polímeros |
| dc.subject.other.spa.fl_str_mv |
Polimerización |
| dc.subject.other.eng.fl_str_mv |
Polymerization |
| dc.subject.proposal.spa.fl_str_mv |
Resorcinarenos Sistemas huésped–hospedero Interacciones moleculares L-carnitina Cuantificación |
| dc.subject.proposal.eng.fl_str_mv |
Resorcinarenes Host-guest systems Molecular interactions L-carnitine Quantification |
| dc.subject.spines.spa.fl_str_mv |
Química de los polímeros |
| description |
ilustraciones, diagramas, fotografías, tablas |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2023-01-12T16:16:04Z |
| dc.date.available.none.fl_str_mv |
2023-01-12T16:16:04Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/82893 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/82893 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Res. 29, 70–74 (2009). Scafidi, S., Racz, J., Hazelton, J., McKenna, M. C. & Fiskum, G. Neuroprotection by acetyl-L-carnitine after traumatic injury to the immature rat brain. Dev. Neurosci. 32, 480–487 (2011). Zhang, R. et al. Neuroprotective effects of pre-treament with L-carnitine and Acetyl L-carnitine on ischemic injury in vivo and in vitro. Int. J. Mol. Sci. 13, 2078–2090 (2012) Patel, S. P., Sullivan, P. G., Lyttle, T. S., Magnuson, D. S. K. & Rabchevsky, A. G. Acetyl-l-carnitine treatment following spinal cord injury improves mitochondrial function correlated with remarkable tissue sparing and functional recovery. Neuroscience 210, 296–307 (2012). Kocsis, K. et al. Acetyl-L-carnitine and oxaloacetate in post-treatment against LTP impairment in a rat ischemia model. An in vitro electrophysiological study. J. Neural Transm. 122, 867–872 (2015). Hota, S. K., Chaurasia, O. P. & Singh, S. B. Acetyl-L-carnitine mediated neuroprotection during hypoxia is attributed to ERK1/2-Nrf2-regulated mitochondrial biosynthesis. Hippocampus 22, 723–736 (2012). Barhwal, K., Hota, S. K., Prasad, D., Singh, S. B. & Ilavazhagan, G. Hypoxia induced deactivation of NGF-mediated ERK1/2 signaling in hippocampal cells: Neuroprotection by acetyl-L-carnitine. J. Neurosci. Res. 86, 2705–2721 (2008). Ishii, T.; Shimpo, Y.; Matsuoka, Y.; Kinoshit, K. 2000.pdf. Wainwright, M. S., Mannix, M. K., Brown, J. & Stumpf, D. A. L-Carnitine Reduces Brain Injury after Hypoxia-Ischemia in Newborn Rats. Pediatr. Res. 54, 688–695 (2003). Wainwright, M. S., Kohli, R., Whitington, P. F. & Chace, D. H. Carnitine treatment inhibits increases in cerebral carnitine esters and glutamate detected by mass spectrometry after hypoxia-ischemia in newborn rats. Stroke 37, 524–530 (2006). Roe, C. R. et al. Metabolic response to carnitine in methylmalonic aciduria. Arch. Dis. Child. 58, 916–920 (1983). Vieira Neto, E. et al. 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Methacrylate monoliths prepared from various hydrophobic and hydrophilic monomers - Structural and chromatographic characteristics. J. Sep. Sci. 26, 322–330 (2003). Ermakova, A. M. et al. Nanoconjugates of a calixresorcinarene derivative with methoxy poly(ethylene glycol) fragments for drug encapsulation. Beilstein J. Nanotechnol. 9, 2057–2070 (2018). |
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Reconocimiento 4.0 Internacional |
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Reconocimiento 4.0 Internacional Derechos reservados al autor, 2022 http://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
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112 páginas |
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Universidad Nacional de Colombia |
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Bogotá - Ciencias - Maestría en Ciencias - Química |
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Facultad de Ciencias |
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Bogotá, Colombia |
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Universidad Nacional de Colombia - Sede Bogotá |
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Universidad Nacional de Colombia |
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Reconocimiento 4.0 InternacionalDerechos reservados al autor, 2022http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Maldonado Villamil, Mauricio3a378aa45195548db31da075813fbd30600Ramírez Perdomo, Gabriel Andrésd530233ad839875fc3089d7ebb90abf0Aplicaciones Analíticas de Compuestos Orgánicos (Aaco)2023-01-12T16:16:04Z2023-01-12T16:16:04Z2022https://repositorio.unal.edu.co/handle/unal/82893Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografías, tablasEste trabajo de investigación tiene como objetivo la síntesis y caracterización de resorcinarenos tipo corona para modificar una superficie polimérica con base en metacrilatos y evaluar la interacción molecular y su capacidad de preconcentración con L-carnitina. La investigación se realizó en cinco etapas: En la primera etapa se desarrolló la síntesis de los resorcinarenos de partida entre el resorcinol y propanal y/o heptanal obteniendo como resultado los productos C-tetra(etil)calix[4]resorcinareno (1A) y C-tetra(hexil)calix[4]resorcinareno (1B), los cuales fueron caracterizados por diferentes técnicas instrumentales tales como IR-ATR, RMN-1H, RMN-13C y espectrometría de masas obteniendo como un único confórmero el tipo corona de gran utilidad por su facilidad de formar sistemas tipo host-guest. En la segunda etapa se evaluó la interacción host-guest (huésped-hospedero) en solución entre los resorcinarenos C-tetra(etil)calix[4]resorcinareno (1A) y C-tetra(hexil) calix[4]resorcinareno (1B) con L-carnitina (1C),este se realizó en disolucion acuosa donde se evaluó mediante RMN-1H y espectrometría de masas evidenciando una interacción entre ellos debido a la cavidad existente entre los resorcinarenos gracias a sus interacciones tipo π. La tercera etapa consistió en la copolimerización entre el butilmetacrilato (BMA) y etilendimetacrilato (EDMA) y su modificación física con los resorcinarenos (1A y 1B) el material polímero se caracterizó a través de IR y SEM – MEB En la cuarta etapa se evalúan las variables de tiempo, solvente, volumen de carga, concentración y pH. Elegidas las mejores condiciones estas se aplican en la última etapa para la cuantificación de la L-carnitina (1C) partiendo del material polímero modificado con resorcinarenos (1A y 1B) mediante cromatografía líquida acoplada a masas.This research work aims to synthesize and characterize corona-type resorcinarenes to modify a methacrylate-based polymeric surface and to evaluate the molecular interaction and their preconcentration ability with L-carnitine. The research was carried out in five stages: In the first stage, the synthesis of the starting resorcinarenes between resorcinol and propanal and/or heptanal was developed obtaining as a result the products C-tetra(ethyl)calix[4]resorcinarene (1A) and C-tetra(hexyl)calix[4]resorcinarene (1B), which were characterized by different instrumental techniques such as IR-ATR, 1H-NMR, 13C-NMR and mass spectrometry obtaining as a single conformer the crown type of great utility for its ease of forming host-guest systems. In the second stage, the host-guest interaction in solution between the resorcinarenes C-tetra(ethyl)calix[4]resorcinarene (1A) and C-tetra(hexyl)calix[4]resorcinarene (1B) with L-carnitine (1C) was evaluated, This was performed in aqueous solution where it was evaluated by 1H-NMR and mass spectrometry evidencing an interaction between them due to the existing cavity between the resorcinarenes thanks to their π-type interactions. The third stage consisted of the copolymerization between butylmethacrylate (BMA) and ethylenedimethacrylate (EDMA) and its physical modification with resorcinarenes (1A and 1B) the polymer material was characterized by IR and SEM-MEB. In the fourth stage, the variables of time, solvent, loading volume, concentration and pH are evaluated. Once the best conditions are chosen, these are applied in the last stage for the quantification of L-carnitine (1C) from the polymer material modified with resorcinarenes (1A and 1B) by mass-coupled liquid chromatography.MaestríaMagíster en Ciencias - QuímicaSíntesis orgánica112 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::547 - Química orgánicaPolimerizaciónPolymerizationResorcinarenosSistemas huésped–hospederoInteracciones molecularesL-carnitinaCuantificaciónResorcinarenesHost-guest systemsMolecular interactionsL-carnitineQuantificationQuímica de los polímerosObtención de una superficie polimérica con base en metacrilatos modificada con resorcinarenos y evaluación de su aplicación en la preconcentración de carnitina por la técnica de extracción en fase sólidaObtaining a methacrylate-based polymeric surface modified with resorcinarenes and evaluation of its application in the preconcentration of carnitine by the solid phase extraction techniqueTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMPitt, M. 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Nanotechnol. 9, 2057–2070 (2018).EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/82893/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53ORIGINAL1026289725.2022.pdf1026289725.2022.pdfTesis de Maestría en Ciencias - Químicaapplication/pdf3920923https://repositorio.unal.edu.co/bitstream/unal/82893/4/1026289725.2022.pdfa46659df72198479a8fba0b190cd4768MD54THUMBNAIL1026289725.2022.pdf.jpg1026289725.2022.pdf.jpgGenerated Thumbnailimage/jpeg5355https://repositorio.unal.edu.co/bitstream/unal/82893/5/1026289725.2022.pdf.jpg5ad5e48320ee5313eff7434ed93cdfabMD55unal/82893oai:repositorio.unal.edu.co:unal/828932023-08-12 23:03:48.658Repositorio Institucional Universidad Nacional de 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