Gene silencing and applications for functional gene validation: the case of geminiviruses
Plants are able to recognize and degrade double-strand RNA molecules employing the mechanism of post-transcriptional gene silencing (PTGS). PTGS is both a vegetal defense strategy against viral infections and a conserved eukaryotic mechanism for regulate endogenous gene expression. The VIGS methodol...
- Autores:
-
López, Camilo Ernesto
Cortés, Simón Pedro
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2011
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/29619
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/29619
http://bdigital.unal.edu.co/19667/
http://bdigital.unal.edu.co/19667/2/
- Palabra clave:
- gene silencing
functional genomics
Geminivirus
Manihot esculenta.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López, Camilo Ernesto5845cefc-bce3-4bba-8230-998bac1dc014300Cortés, Simón Pedro263546c9-9173-41d4-90d7-42e5e446d27b3002019-06-26T13:43:04Z2019-06-26T13:43:04Z2011https://repositorio.unal.edu.co/handle/unal/29619http://bdigital.unal.edu.co/19667/http://bdigital.unal.edu.co/19667/2/Plants are able to recognize and degrade double-strand RNA molecules employing the mechanism of post-transcriptional gene silencing (PTGS). PTGS is both a vegetal defense strategy against viral infections and a conserved eukaryotic mechanism for regulate endogenous gene expression. The VIGS methodology(Virus Induced Gene Silencing) uses this mechanism to selectively silence genes employing viral vectors, which contain the target gene, becoming a tool for functional gene validation. Geminiviridae family, with the Begomovirus, Curtovirus, Mastrevirus and Topocuvirus genera, encompass viruses of circular, single-strand DNA packed in icosahedric geminated particles, and some of them had been evolved to infect particular plant species. Classification of these genera is based in the genome organization, the type of host plants and the insect vector that transmits the virus. Geminiviruses are able to induce gene silencing (GS) and therefore they have been used to develop VIGS–based methodologies for functional gene silencing. This review describes the molecular mechanism of gene silencing, with emphasis in gene silencing induced by Geminiviruses and the applications for a staple crop as cassava.application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/15699Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965López, Camilo Ernesto and Cortés, Simón Pedro (2011) Gene silencing and applications for functional gene validation: the case of geminiviruses. Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965 .Gene silencing and applications for functional gene validation: the case of geminivirusesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTgene silencingfunctional genomicsGeminivirusManihot esculenta.ORIGINAL15699-48173-1-SP.pdfapplication/pdf256558https://repositorio.unal.edu.co/bitstream/unal/29619/1/15699-48173-1-SP.pdfe358497267b67554885dfb25d42d4bc1MD5115699-102561-2-PB.pdfapplication/pdf394940https://repositorio.unal.edu.co/bitstream/unal/29619/2/15699-102561-2-PB.pdfaca23f286abfa8fa0c4fbc3656c72297MD52THUMBNAIL15699-48173-1-SP.pdf.jpg15699-48173-1-SP.pdf.jpgGenerated Thumbnailimage/jpeg4900https://repositorio.unal.edu.co/bitstream/unal/29619/3/15699-48173-1-SP.pdf.jpg96f4506c0cb48e8b2fd298fa8bf2d9f1MD5315699-102561-2-PB.pdf.jpg15699-102561-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg8596https://repositorio.unal.edu.co/bitstream/unal/29619/4/15699-102561-2-PB.pdf.jpg3349e252a05793d48b23fcf7da3bbd13MD54unal/29619oai:repositorio.unal.edu.co:unal/296192022-11-22 23:04:13.117Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Gene silencing and applications for functional gene validation: the case of geminiviruses |
title |
Gene silencing and applications for functional gene validation: the case of geminiviruses |
spellingShingle |
Gene silencing and applications for functional gene validation: the case of geminiviruses gene silencing functional genomics Geminivirus Manihot esculenta. |
title_short |
Gene silencing and applications for functional gene validation: the case of geminiviruses |
title_full |
Gene silencing and applications for functional gene validation: the case of geminiviruses |
title_fullStr |
Gene silencing and applications for functional gene validation: the case of geminiviruses |
title_full_unstemmed |
Gene silencing and applications for functional gene validation: the case of geminiviruses |
title_sort |
Gene silencing and applications for functional gene validation: the case of geminiviruses |
dc.creator.fl_str_mv |
López, Camilo Ernesto Cortés, Simón Pedro |
dc.contributor.author.spa.fl_str_mv |
López, Camilo Ernesto Cortés, Simón Pedro |
dc.subject.proposal.spa.fl_str_mv |
gene silencing functional genomics Geminivirus Manihot esculenta. |
topic |
gene silencing functional genomics Geminivirus Manihot esculenta. |
description |
Plants are able to recognize and degrade double-strand RNA molecules employing the mechanism of post-transcriptional gene silencing (PTGS). PTGS is both a vegetal defense strategy against viral infections and a conserved eukaryotic mechanism for regulate endogenous gene expression. The VIGS methodology(Virus Induced Gene Silencing) uses this mechanism to selectively silence genes employing viral vectors, which contain the target gene, becoming a tool for functional gene validation. Geminiviridae family, with the Begomovirus, Curtovirus, Mastrevirus and Topocuvirus genera, encompass viruses of circular, single-strand DNA packed in icosahedric geminated particles, and some of them had been evolved to infect particular plant species. Classification of these genera is based in the genome organization, the type of host plants and the insect vector that transmits the virus. Geminiviruses are able to induce gene silencing (GS) and therefore they have been used to develop VIGS–based methodologies for functional gene silencing. This review describes the molecular mechanism of gene silencing, with emphasis in gene silencing induced by Geminiviruses and the applications for a staple crop as cassava. |
publishDate |
2011 |
dc.date.issued.spa.fl_str_mv |
2011 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-26T13:43:04Z |
dc.date.available.spa.fl_str_mv |
2019-06-26T13:43:04Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/29619 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/19667/ http://bdigital.unal.edu.co/19667/2/ |
url |
https://repositorio.unal.edu.co/handle/unal/29619 http://bdigital.unal.edu.co/19667/ http://bdigital.unal.edu.co/19667/2/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/agrocol/article/view/15699 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
dc.relation.ispartofseries.none.fl_str_mv |
Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965 |
dc.relation.references.spa.fl_str_mv |
López, Camilo Ernesto and Cortés, Simón Pedro (2011) Gene silencing and applications for functional gene validation: the case of geminiviruses. Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial |
institution |
Universidad Nacional de Colombia |
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