Gene silencing and applications for functional gene validation: the case of geminiviruses

Plants are able to recognize and degrade double-strand RNA molecules employing the mechanism of post-transcriptional gene silencing (PTGS). PTGS is both a vegetal defense strategy against viral infections and a conserved eukaryotic mechanism for regulate endogenous gene expression. The VIGS methodol...

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Autores:
López, Camilo Ernesto
Cortés, Simón Pedro
Tipo de recurso:
Article of journal
Fecha de publicación:
2011
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/29619
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/29619
http://bdigital.unal.edu.co/19667/
http://bdigital.unal.edu.co/19667/2/
Palabra clave:
gene silencing
functional genomics
Geminivirus
Manihot esculenta.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López, Camilo Ernesto5845cefc-bce3-4bba-8230-998bac1dc014300Cortés, Simón Pedro263546c9-9173-41d4-90d7-42e5e446d27b3002019-06-26T13:43:04Z2019-06-26T13:43:04Z2011https://repositorio.unal.edu.co/handle/unal/29619http://bdigital.unal.edu.co/19667/http://bdigital.unal.edu.co/19667/2/Plants are able to recognize and degrade double-strand RNA molecules employing the mechanism of post-transcriptional gene silencing (PTGS). PTGS is both a vegetal defense strategy against viral infections and a conserved eukaryotic mechanism for regulate endogenous gene expression. The VIGS methodology(Virus Induced Gene Silencing) uses this mechanism to selectively silence genes employing viral vectors, which contain the target gene, becoming a tool for functional gene validation. Geminiviridae family, with the Begomovirus, Curtovirus, Mastrevirus and Topocuvirus genera, encompass viruses of circular, single-strand DNA packed in icosahedric geminated particles, and some of them had been evolved to infect particular plant species. Classification of these genera is based in the genome organization, the type of host plants and the insect vector that transmits the virus. Geminiviruses are able to induce gene silencing (GS) and therefore they have been used to develop VIGS–based methodologies for functional gene silencing. This review describes the molecular mechanism of gene silencing, with emphasis in gene silencing induced by Geminiviruses and the applications for a staple crop as cassava.application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/15699Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965López, Camilo Ernesto and Cortés, Simón Pedro (2011) Gene silencing and applications for functional gene validation: the case of geminiviruses. Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965 .Gene silencing and applications for functional gene validation: the case of geminivirusesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTgene silencingfunctional genomicsGeminivirusManihot esculenta.ORIGINAL15699-48173-1-SP.pdfapplication/pdf256558https://repositorio.unal.edu.co/bitstream/unal/29619/1/15699-48173-1-SP.pdfe358497267b67554885dfb25d42d4bc1MD5115699-102561-2-PB.pdfapplication/pdf394940https://repositorio.unal.edu.co/bitstream/unal/29619/2/15699-102561-2-PB.pdfaca23f286abfa8fa0c4fbc3656c72297MD52THUMBNAIL15699-48173-1-SP.pdf.jpg15699-48173-1-SP.pdf.jpgGenerated Thumbnailimage/jpeg4900https://repositorio.unal.edu.co/bitstream/unal/29619/3/15699-48173-1-SP.pdf.jpg96f4506c0cb48e8b2fd298fa8bf2d9f1MD5315699-102561-2-PB.pdf.jpg15699-102561-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg8596https://repositorio.unal.edu.co/bitstream/unal/29619/4/15699-102561-2-PB.pdf.jpg3349e252a05793d48b23fcf7da3bbd13MD54unal/29619oai:repositorio.unal.edu.co:unal/296192022-11-22 23:04:13.117Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Gene silencing and applications for functional gene validation: the case of geminiviruses
title Gene silencing and applications for functional gene validation: the case of geminiviruses
spellingShingle Gene silencing and applications for functional gene validation: the case of geminiviruses
gene silencing
functional genomics
Geminivirus
Manihot esculenta.
title_short Gene silencing and applications for functional gene validation: the case of geminiviruses
title_full Gene silencing and applications for functional gene validation: the case of geminiviruses
title_fullStr Gene silencing and applications for functional gene validation: the case of geminiviruses
title_full_unstemmed Gene silencing and applications for functional gene validation: the case of geminiviruses
title_sort Gene silencing and applications for functional gene validation: the case of geminiviruses
dc.creator.fl_str_mv López, Camilo Ernesto
Cortés, Simón Pedro
dc.contributor.author.spa.fl_str_mv López, Camilo Ernesto
Cortés, Simón Pedro
dc.subject.proposal.spa.fl_str_mv gene silencing
functional genomics
Geminivirus
Manihot esculenta.
topic gene silencing
functional genomics
Geminivirus
Manihot esculenta.
description Plants are able to recognize and degrade double-strand RNA molecules employing the mechanism of post-transcriptional gene silencing (PTGS). PTGS is both a vegetal defense strategy against viral infections and a conserved eukaryotic mechanism for regulate endogenous gene expression. The VIGS methodology(Virus Induced Gene Silencing) uses this mechanism to selectively silence genes employing viral vectors, which contain the target gene, becoming a tool for functional gene validation. Geminiviridae family, with the Begomovirus, Curtovirus, Mastrevirus and Topocuvirus genera, encompass viruses of circular, single-strand DNA packed in icosahedric geminated particles, and some of them had been evolved to infect particular plant species. Classification of these genera is based in the genome organization, the type of host plants and the insect vector that transmits the virus. Geminiviruses are able to induce gene silencing (GS) and therefore they have been used to develop VIGS–based methodologies for functional gene silencing. This review describes the molecular mechanism of gene silencing, with emphasis in gene silencing induced by Geminiviruses and the applications for a staple crop as cassava.
publishDate 2011
dc.date.issued.spa.fl_str_mv 2011
dc.date.accessioned.spa.fl_str_mv 2019-06-26T13:43:04Z
dc.date.available.spa.fl_str_mv 2019-06-26T13:43:04Z
dc.type.spa.fl_str_mv Artículo de revista
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url https://repositorio.unal.edu.co/handle/unal/29619
http://bdigital.unal.edu.co/19667/
http://bdigital.unal.edu.co/19667/2/
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dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/agrocol/article/view/15699
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.ispartofseries.none.fl_str_mv Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965
dc.relation.references.spa.fl_str_mv López, Camilo Ernesto and Cortés, Simón Pedro (2011) Gene silencing and applications for functional gene validation: the case of geminiviruses. Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 Agronomía Colombiana; Vol. 29, núm. 1 (2011); 27-34 2357-3732 0120-9965 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
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dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
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eu_rights_str_mv openAccess
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