Anti-pepsin activity of silicon dioxide nanoparticles
SiO2NPs as an inhibitor of pepsin enzyme for treatment of gastro-esophageal reflux disease (GERD) was used. Silicon dioxide nanoparticles (pepsin coated SiO2NPs) are among the safest nanoparticles that can be used inside the human body. The activity of pepsin before and after the addition of certain...
- Autores:
-
Al-Hakeim, Hussein Kadhem
Jasem, Khlowd Mohammed
Moustafa, Shatha Rouf
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/66279
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/66279
http://bdigital.unal.edu.co/67303/
- Palabra clave:
- 54 Química y ciencias afines / Chemistry
GERD
SiO2 nanoparticles
pepsin
and enzyme inhibition
GERD, nanopartículas de SiO2
pepsina
inhibición enzimática
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Anti-pepsin activity of silicon dioxide nanoparticles |
| title |
Anti-pepsin activity of silicon dioxide nanoparticles |
| spellingShingle |
Anti-pepsin activity of silicon dioxide nanoparticles 54 Química y ciencias afines / Chemistry GERD SiO2 nanoparticles pepsin and enzyme inhibition GERD, nanopartículas de SiO2 pepsina inhibición enzimática |
| title_short |
Anti-pepsin activity of silicon dioxide nanoparticles |
| title_full |
Anti-pepsin activity of silicon dioxide nanoparticles |
| title_fullStr |
Anti-pepsin activity of silicon dioxide nanoparticles |
| title_full_unstemmed |
Anti-pepsin activity of silicon dioxide nanoparticles |
| title_sort |
Anti-pepsin activity of silicon dioxide nanoparticles |
| dc.creator.fl_str_mv |
Al-Hakeim, Hussein Kadhem Jasem, Khlowd Mohammed Moustafa, Shatha Rouf |
| dc.contributor.author.spa.fl_str_mv |
Al-Hakeim, Hussein Kadhem Jasem, Khlowd Mohammed Moustafa, Shatha Rouf |
| dc.subject.ddc.spa.fl_str_mv |
54 Química y ciencias afines / Chemistry |
| topic |
54 Química y ciencias afines / Chemistry GERD SiO2 nanoparticles pepsin and enzyme inhibition GERD, nanopartículas de SiO2 pepsina inhibición enzimática |
| dc.subject.proposal.spa.fl_str_mv |
GERD SiO2 nanoparticles pepsin and enzyme inhibition GERD, nanopartículas de SiO2 pepsina inhibición enzimática |
| description |
SiO2NPs as an inhibitor of pepsin enzyme for treatment of gastro-esophageal reflux disease (GERD) was used. Silicon dioxide nanoparticles (pepsin coated SiO2NPs) are among the safest nanoparticles that can be used inside the human body. The activity of pepsin before and after the addition of certain amounts of the NPs to the reaction mixture was measured spectrophotometrically. Furthermore, these experiments were repeated at different temperatures, different weights of NPs, and different ionic strengths. The kinetic aparameters (Km and Vmax) of the pepsin catalyzed reactions were calculated from the Lineweaver-Burke plots. The results showed that there is a significant reduction of pepsin activity by SiO2NPs (Vmax of free pepsin = 4.82 U and Vmax of the immobilizedpepsin = 2.90 U). The results also indicated that the presence of ionic strength causes remarkable reduction of pepsin activity. It can be concluded the best condition for inhibition of pepsin activity is by using a combination of SiO2NPs and high concentration NaCl at 37 °C. |
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2016 |
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2016-09-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-03T01:49:30Z |
| dc.date.available.spa.fl_str_mv |
2019-07-03T01:49:30Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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ISSN: 2357-3791 |
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https://repositorio.unal.edu.co/handle/unal/66279 |
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http://bdigital.unal.edu.co/67303/ |
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ISSN: 2357-3791 |
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spa |
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spa |
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https://revistas.unal.edu.co/index.php/rcolquim/article/view/58760 |
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Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Química Revista Colombiana de Química |
| dc.relation.references.spa.fl_str_mv |
Al-Hakeim, Hussein Kadhem and Jasem, Khlowd Mohammed and Moustafa, Shatha Rouf (2016) Anti-pepsin activity of silicon dioxide nanoparticles. Revista Colombiana de Química, 45 (3). pp. 5-11. ISSN 2357-3791 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Química |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Al-Hakeim, Hussein Kadhemc7b415c6-23ca-44ef-be83-2aabfe66bc40300Jasem, Khlowd Mohammed2e49654b-b5be-40a5-bf26-27547c10cb66300Moustafa, Shatha Rouf2e6d106e-242d-40f1-88ef-2fbf7531298b3002019-07-03T01:49:30Z2019-07-03T01:49:30Z2016-09-01ISSN: 2357-3791https://repositorio.unal.edu.co/handle/unal/66279http://bdigital.unal.edu.co/67303/SiO2NPs as an inhibitor of pepsin enzyme for treatment of gastro-esophageal reflux disease (GERD) was used. Silicon dioxide nanoparticles (pepsin coated SiO2NPs) are among the safest nanoparticles that can be used inside the human body. The activity of pepsin before and after the addition of certain amounts of the NPs to the reaction mixture was measured spectrophotometrically. Furthermore, these experiments were repeated at different temperatures, different weights of NPs, and different ionic strengths. The kinetic aparameters (Km and Vmax) of the pepsin catalyzed reactions were calculated from the Lineweaver-Burke plots. The results showed that there is a significant reduction of pepsin activity by SiO2NPs (Vmax of free pepsin = 4.82 U and Vmax of the immobilizedpepsin = 2.90 U). The results also indicated that the presence of ionic strength causes remarkable reduction of pepsin activity. It can be concluded the best condition for inhibition of pepsin activity is by using a combination of SiO2NPs and high concentration NaCl at 37 °C.Se usaron nanopartículas de dióxido de silicio como inhibidores de la pepsina para el tratamiento del reflujo gastroesofágico (GERD). Estas nanopartículas (SiO2NPs recubiertas de pepsina) son unas de las más seguras y pueden usarse en el cuerpo humano. Se midió a través de espectrofotometría la actividad de la pepsina antes y después de añadir cierta cantidad de NPs a la mezcla reactante. Adicionalmente, se repitieron estas pruebas a diferentes temperaturas, variando el peso de las NPs y la fuerza iónica. Se calcularon los parámetros cinéticos (Km y Vmax) de las reacciones catalizadas con pepsina a través de las gráficas de Lineweaver-Burke. Los resultados mostraron que, usando SiO2NPs (Vmax de pepsina libre = 4.82 U y Vmax de pepsina inmovilizada = 2.90 U) y a través de la presencia de fuerza iónica, la actividad enzimática se reduce significativamente. Se concluye que la mejor condición para inhibir la actividad enzimática es usando una combinación de SiO2NPs y una alta concentración de NaCl a 37 °C.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Químicahttps://revistas.unal.edu.co/index.php/rcolquim/article/view/58760Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de QuímicaRevista Colombiana de QuímicaAl-Hakeim, Hussein Kadhem and Jasem, Khlowd Mohammed and Moustafa, Shatha Rouf (2016) Anti-pepsin activity of silicon dioxide nanoparticles. Revista Colombiana de Química, 45 (3). pp. 5-11. ISSN 2357-379154 Química y ciencias afines / ChemistryGERDSiO2 nanoparticlespepsinand enzyme inhibitionGERD, nanopartículas de SiO2pepsinainhibición enzimáticaAnti-pepsin activity of silicon dioxide nanoparticlesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL58760-318164-1-PB.pdfapplication/pdf1979646https://repositorio.unal.edu.co/bitstream/unal/66279/1/58760-318164-1-PB.pdf5106423c5a1314f4a66420107eb71e8bMD51THUMBNAIL58760-318164-1-PB.pdf.jpg58760-318164-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8732https://repositorio.unal.edu.co/bitstream/unal/66279/2/58760-318164-1-PB.pdf.jpg9a467a9f32a77aa98d6195ac9c533b71MD52unal/66279oai:repositorio.unal.edu.co:unal/662792024-05-15 23:09:11.933Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |