Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control

ilustraciones, diagramas

Autores:
Saldarriaga Gómez, Catalina
Tipo de recurso:
Fecha de publicación:
2024
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/86640
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/86640
https://repositorio.unal.edu.co/
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630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)
Mecanismos de defensa
Antracnosis de la judía
Colletotrichum lindemuthianum
defence mechanisms
bean anthracnose
Colletotrichum lindemuthianum
Antracnosis
Genes de defensa
Factor de virulencia
Fosfito de potasio
Anthracnose
Defense genes
Virulence factor
Potassium phosphite
Rights
openAccess
License
Reconocimiento 4.0 Internacional
id UNACIONAL2_12472fe1730da3f5aca0217d8a6a3a49
oai_identifier_str oai:repositorio.unal.edu.co:unal/86640
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
dc.title.translated.eng.fl_str_mv Association of the virulence of Colletotrichum lindemuthianum with the defense response in common beans under an alternative control
title Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
spellingShingle Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)
Mecanismos de defensa
Antracnosis de la judía
Colletotrichum lindemuthianum
defence mechanisms
bean anthracnose
Colletotrichum lindemuthianum
Antracnosis
Genes de defensa
Factor de virulencia
Fosfito de potasio
Anthracnose
Defense genes
Virulence factor
Potassium phosphite
title_short Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
title_full Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
title_fullStr Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
title_full_unstemmed Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
title_sort Asociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de control
dc.creator.fl_str_mv Saldarriaga Gómez, Catalina
dc.contributor.advisor.spa.fl_str_mv Gonzalez Almario, Adriana
dc.contributor.author.spa.fl_str_mv Saldarriaga Gómez, Catalina
dc.contributor.researchgroup.spa.fl_str_mv Genética de Rasgos de Interés Agronómico
dc.contributor.orcid.spa.fl_str_mv 0000-0002-8563-5210
dc.subject.ddc.spa.fl_str_mv 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)
topic 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)
Mecanismos de defensa
Antracnosis de la judía
Colletotrichum lindemuthianum
defence mechanisms
bean anthracnose
Colletotrichum lindemuthianum
Antracnosis
Genes de defensa
Factor de virulencia
Fosfito de potasio
Anthracnose
Defense genes
Virulence factor
Potassium phosphite
dc.subject.agrovoc.spa.fl_str_mv Mecanismos de defensa
Antracnosis de la judía
Colletotrichum lindemuthianum
dc.subject.agrovoc.eng.fl_str_mv defence mechanisms
bean anthracnose
Colletotrichum lindemuthianum
dc.subject.proposal.spa.fl_str_mv Antracnosis
Genes de defensa
Factor de virulencia
Fosfito de potasio
dc.subject.proposal.eng.fl_str_mv Anthracnose
Defense genes
Virulence factor
Potassium phosphite
description ilustraciones, diagramas
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-07-29T18:54:29Z
dc.date.available.none.fl_str_mv 2024-07-29T18:54:29Z
dc.date.issued.none.fl_str_mv 2024
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/86640
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/86640
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv Agrosavia
Agrovoc
dc.relation.references.spa.fl_str_mv Alvarez-Diaz, J.C., Laugé, R., Delannoy, E., Huguet, S., Paysant-Le Roux, C., Gratias, A., Geffroy, V. (2022). Genome-wide transcriptomic analysis of the effects of infection with the hemibiotrophic fungus Colletotrichum lindemuthianum on common bean. Plants, 11(15), 1995.
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dc.rights.license.spa.fl_str_mv Reconocimiento 4.0 Internacional
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http://creativecommons.org/licenses/by/4.0/
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dc.format.extent.spa.fl_str_mv xviii, 96 páginas
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv Bogotá - Ciencias Agrarias - Maestría en Ciencias Agrarias
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias Agrarias
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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spelling Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gonzalez Almario, Adriana2095714a2ee0a49a87d2319bc2e287b4600Saldarriaga Gómez, Catalina9ef435380972297f53aa71302725581e600Genética de Rasgos de Interés Agronómico0000-0002-8563-52102024-07-29T18:54:29Z2024-07-29T18:54:29Z2024https://repositorio.unal.edu.co/handle/unal/86640Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasLa antracnosis ocasionada por el hongo Colletotrichum lindemuthianum es una de las enfermedades más limitantes en el cultivo de frijol (Phaseolus vulgaris L.) ocasionando una disminución en su rendimiento. Entre las alternativas de manejo se ha implementado el uso de compuestos como los fosfitos los cuales pueden tener un efecto en la planta induciendo sus mecanismos de resistencia como también un efecto fungicida inhibiendo el crecimiento micelial y la germinación de las esporas del hongo. Por tanto, el objetivo de este trabajo fue evaluar la virulencia de C. lindemuthianum y asociarla con la respuesta de defensa en frijol bajo una alternativa de control a base de una solución de fosfito de potasio. Para esto, los aislados Cl(a) y el Cl(b) de C. lindemuthianum fueron caracterizados macro y microscópicamente y su virulencia fue evaluada en plantas de frijol del cultivar Sutagao con y sin aplicación previa de una solución de fosfito de potasio antes de la inoculación del patógeno. Así mismo, se evaluó la expresión de los genes PR1, PR4, PR3 y POD asociados con la defensa de la planta y la del factor de virulencia Cac1 del hongo, a las 0, 24, 48, 72 y 96 horas posteriores a la infección (hpi). Como resultados se obtuvo que el aislado Cl(a) desarrolló conidias y acérvulos en medio PDA, características que no fueron detectadas en Cl(b). Adicionalmente, el aislado Cl(a) al ser inoculado en las plantas de frijol del cultivar Sutagao fue más virulento que Cl(b), al generar el valor más alto de severidad y del área bajo la curva del progreso de la enfermedad (AUDPC). A nivel molecular, el aislado Cl(b) indujo un valor significativamente mayor en la expresión de los genes de defensa evaluados a diferencia del aislado Cl(a). No obstante, el factor de virulencia Cac1 de C. lindemuthianum presentó un valor de expresión significativamente mayor en el aislado Cl(a) comparado con Cl(b). Con respecto al tratamiento de las plantas con solución de fosfito de potasio previo a la infección con el hongo, se evidenció una disminución en el tamaño de las lesiones necróticas, se detectó un aumento en la expresión de los genes PR1, PR3, PR4 y POD con respecto a las plantas no tratadas y el patógeno expresó el gen Cac1 contribuyendo con su virulencia. Con base en estos resultados, se concluye que la variedad de frijol Sutagao presenta una menor expresión de genes de defensa cuando está frente a un aislamiento de C. lindemuthianum que expresa el factor de virulencia Cac1 en las etapas iniciales de la infección. Así mismo, se evidenció que la aplicación de una solución de fosfito de potasio previa a la infección con el patógeno, regula positivamente la expresión de genes de defensa de la planta contribuyendo a una disminución en la severidad de la enfermedad. (Texto tomado de la fuente).Anthracnose caused by the fungus Colletotrichum lindemuthianum is one of the most limiting diseases in bean cultivation (Phaseolus vulgaris L.), leading to a decrease in yield. Among the management alternatives, the use of compounds such as phosphites has been implemented, which can affect the plant by inducing its resistance mechanisms as well as a fungicidal effect, inhibiting the mycelial growth and spore germination of the fungus. Therefore, the objective of this study was to evaluate the virulence of C. lindemuthianum and associate it with the defense response in beans under a control alternative based on a solution of potassium phosphite. For this purpose, the isolates Cl(a) and Cl(b) of C. lindemuthianum were characterized macro and microscopically, and their virulence was evaluated in bean plants of the Sutagao cultivar, with and without prior application of a potassium phosphite solution before inoculation with the pathogen. Likewise, the expression of the genes PR1, PR4, PR3, and POD associated with plant defense, and the virulence factor Cac1 of the fungus, was evaluated at 0, 24, 48, 72, and 96 hours postinfection (hpi). Results showed that the Cl(a) isolate developed conidia and acervuli in PDA medium, characteristics not detected in Cl(b). Additionally, Cl(a), when inoculated into Sutagao bean plants, was more virulent than Cl(b), generating the highest severity value and area under the disease progress curve (AUDPC). At the molecular level, the Cl(b) isolate induced a significantly higher expression of the bean defense genes evaluated compared to the Cl(a) isolate. However, the Cac1 gene of C. lindemuthianum showed a significantly higher expression value in the Cl(a) isolate compared to Cl(b). Regarding the treatment of plants with a potassium phosphite solution before infection with the fungus, a decrease in the size of necrotic lesions was observed, and an increase in the expression of the PR1, PR3, PR4 and POD genes was detected compared to untreated plants. Based on these results, it is concluded that the Sutagao bean variety exhibits a lower expression of defense genes when confronted with an isolate of C. lindemuthianum expressing the Cac1 virulence factor in the early stages of infection. Similarly, it was observed that the application of a potassium phosphite solution before to infection with the pathogen positively regulates the expression of plant defense genes, contributing to a decrease in the severity of the disease.MaestríaMagíster en Ciencias AgrariasFitopatologíaxviii, 96 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias Agrarias - Maestría en Ciencias AgrariasFacultad de Ciencias AgrariasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales630 - Agricultura y tecnologías relacionadas::635 - Cultivos hortícolas (Horticultura)Mecanismos de defensaAntracnosis de la judíaColletotrichum lindemuthianumdefence mechanismsbean anthracnoseColletotrichum lindemuthianumAntracnosisGenes de defensaFactor de virulenciaFosfito de potasioAnthracnoseDefense genesVirulence factorPotassium phosphiteAsociación de la virulencia de Colletotrichum lindemuthianum con la respuesta de defensa en frijol común bajo una alternativa de controlAssociation of the virulence of Colletotrichum lindemuthianum with the defense response in common beans under an alternative controlTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAgrosaviaAgrovocAlvarez-Diaz, J.C., Laugé, R., Delannoy, E., Huguet, S., Paysant-Le Roux, C., Gratias, A., Geffroy, V. 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Plant Biology BMC, 16(86).EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86640/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1018464790.2024.pdf1018464790.2024.pdfTesis de Maestría en Ciencias Agrariasapplication/pdf10066820https://repositorio.unal.edu.co/bitstream/unal/86640/2/1018464790.2024.pdfba1567d3e569bd623122c1b8093fcc81MD52THUMBNAIL1018464790.2024.pdf.jpg1018464790.2024.pdf.jpgGenerated Thumbnailimage/jpeg5615https://repositorio.unal.edu.co/bitstream/unal/86640/3/1018464790.2024.pdf.jpg55282f4e3c7832c1e045f80e09f9656cMD53unal/86640oai:repositorio.unal.edu.co:unal/866402024-07-29 23:05:41.977Repositorio Institucional Universidad Nacional de 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