Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi
ilustraciones, fotografías a color
- Autores:
-
Castiblanco Quiroga, Nelly Fernanda
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84021
- Palabra clave:
- 570 - Biología::572 - Bioquímica
580 - Plantas
540 - Química y ciencias afines
580 - Plantas::582 - Plantas destacadas por características vegetativas y flores
Claveles
Virus fitopatógenos
Virosis (plantas)
Carnations
Plant viruses
Virus diseases of plants
Fusarium oxysporum f. sp. dianthi
Dianthus caryophyllus L.
Tiamina
Superóxido dismutasa
Tripeptidil peptidasa
Proteínas SIX
Factor de virulencia
Fusarium oxysporum f. sp. dianthi
Dianthus caryophyllus L.
Thiamine
Superoxide dismutase
Tripeptidyl peptidase
SIX proteins
Virulence factor
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi |
| dc.title.translated.eng.fl_str_mv |
Effect of the application of resistance inducers on the in vivo expression of candidate virulence factor genes of the pathogen Fusarium oxysporum f. sp. dianthi in carnation (Dianthus caryophyllus L) |
| title |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi |
| spellingShingle |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi 570 - Biología::572 - Bioquímica 580 - Plantas 540 - Química y ciencias afines 580 - Plantas::582 - Plantas destacadas por características vegetativas y flores Claveles Virus fitopatógenos Virosis (plantas) Carnations Plant viruses Virus diseases of plants Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus L. Tiamina Superóxido dismutasa Tripeptidil peptidasa Proteínas SIX Factor de virulencia Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus L. Thiamine Superoxide dismutase Tripeptidyl peptidase SIX proteins Virulence factor |
| title_short |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi |
| title_full |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi |
| title_fullStr |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi |
| title_full_unstemmed |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi |
| title_sort |
Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi |
| dc.creator.fl_str_mv |
Castiblanco Quiroga, Nelly Fernanda |
| dc.contributor.advisor.none.fl_str_mv |
Ardila Barrantes, Harold Duban |
| dc.contributor.author.none.fl_str_mv |
Castiblanco Quiroga, Nelly Fernanda |
| dc.contributor.researchgroup.spa.fl_str_mv |
Estudio de Actividades Metabólicas Vegetales |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::572 - Bioquímica 580 - Plantas 540 - Química y ciencias afines 580 - Plantas::582 - Plantas destacadas por características vegetativas y flores |
| topic |
570 - Biología::572 - Bioquímica 580 - Plantas 540 - Química y ciencias afines 580 - Plantas::582 - Plantas destacadas por características vegetativas y flores Claveles Virus fitopatógenos Virosis (plantas) Carnations Plant viruses Virus diseases of plants Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus L. Tiamina Superóxido dismutasa Tripeptidil peptidasa Proteínas SIX Factor de virulencia Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus L. Thiamine Superoxide dismutase Tripeptidyl peptidase SIX proteins Virulence factor |
| dc.subject.lemb.spa.fl_str_mv |
Claveles Virus fitopatógenos Virosis (plantas) |
| dc.subject.lemb.eng.fl_str_mv |
Carnations Plant viruses Virus diseases of plants |
| dc.subject.proposal.spa.fl_str_mv |
Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus L. Tiamina Superóxido dismutasa Tripeptidil peptidasa Proteínas SIX Factor de virulencia |
| dc.subject.proposal.eng.fl_str_mv |
Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus L. Thiamine Superoxide dismutase Tripeptidyl peptidase SIX proteins Virulence factor |
| description |
ilustraciones, fotografías a color |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-06-14T23:13:05Z |
| dc.date.available.none.fl_str_mv |
2023-06-14T23:13:05Z |
| dc.date.issued.none.fl_str_mv |
2023-06-15 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84021 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/84021 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
| dc.relation.references.spa.fl_str_mv |
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Purification of a Protein from Culture Filtrates of Fusarium oxysporum that Induces Ethylene and Necrosis in Leaves of Erythroxylum coca. Phytopathology, 85(10), 1250. https://doi.org/10.1094/Phyto-85-1250 Bailey, B. A., Apel-Birkhold, P. C., & Luster, D. G. (2002). Expression of NEP1 by Fusarium oxysporum f. sp. erythroxyli After Gene Replacement and Overexpression Using Polyethylene Glycol-Mediated Transformation. Phytopathology, 92(8), 833–841. https://doi.org/10.1094/PHYTO.2002.92.8.833 Bailey, B. A., Jennings, J. C., & Anderson, J. D. (1997). The 24-kDa protein from Fusarium oxysporum f.sp. erythroxyli: occurrence in related fungi and the effect of growth medium on its production. Canadian Journal of Microbiology, 43(1), 45–55 Balmer, A., Pastor, V., Gamir, J., Flors, V., & Mauch-Mani, B. (2015). The ‘prime- ome’: towards a holistic approach to priming. 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Condiciones de crecimiento del hongo Fusarium oxysporum f. sp. dianthi para la preparación de un potencial inductor de resistencia al marchitamiento vascular del clavel Dianthus caryophyllus L. Universidad Nacional de Colombia. Catanzariti, A.-M., Do, H. T. T., Bru, P., de Sain, M., Thatcher, L. F., Rep, M., & Jones, D. A. (2017). The tomato I gene for Fusarium wilt resistance encodes an atypical leucine-rich repeat receptor-like protein whose function is nevertheless dependent on SOBIR1 and SERK3/BAK1. The Plant Journal, 89(6), 1195– 1209. https://doi.org/10.1111/tpj.13458 Chen, C., Zhang, M.-K., Hu, K.-D., Sun, K.-K., Li, Y.-H., Hu, L.-Y., Chen, X.-Y., Yang, Y., Yang, F., Tang, J., Liu, H.-P., & Zhang, H. (2017). Deletion of Cu/Zn Superoxide Dismutase Gene sodC Reduces Aspergillus niger Virulence on Chinese White Pear. Journal of the American Society for Horticultural Science J. Amer. Soc. Hort. 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Sequence variation in the putative effector gene SIX8 facilitates molecular differentiation of Fusarium oxysporum f. sp. cubense. Plant Pathology, 63(5), 1044–1052. https://doi.org/10.1111/ppa.12184 Gawehns, F., Houterman, P. M., Ichou, F. A., Michielse, C. B., Hijdra, M., Cornelissen, B. J. C., Rep, M., & Takken, F. L. W. (2014). The Fusarium oxysporum Effector Six6 Contributes to Virulence and Suppresses I-2-Mediated Cell Death. Molecular Plant-Microbe Interactions, 27(4), 336–348. https://doi.org/10.1094/MPMI-11-13-0330-R Gawehns, F. K. K. (2014). Function and targets of Fusarium oxysporum effectors. Swammerdam Institute for Life Sciences (SILS). Gawehns, F., Ma, L., Bruning, O., Houterman, P. M., Boeren, S., Cornelissen, B. J. C., Rep, M., & Takken, F. L. W. (2015). The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection. Frontiers in Plant Science, 6, 967. https://doi.org/10.3389/fpls.2015.00967 Gentile, I. A., & Matta, A. (1975). Production of and some effects of ethylene in relation to Fusarium wilt of tomato. Physiological Plant Pathology, 5(1), 27–35. https://doi.org/10.1016/0048-4059(75)90067-3 Gessler, N. N., Aver’yanov, A. A., & Belozerskaya, T. A. (2007). Reactive oxygen species in regulation of fungal development. Biochemistry. Biokhimiia, 72(10), 1091–1109. https://doi.org/10.1134/S0006297907100070 Goellner, K., & Conrath, U. (2008). Priming: it’s all the world to induced disease resistance. European Journal of Plant Pathology, 121(3), 233–242. https://doi.org/10.1007/s10658-007-9251-4 Gómez García, L., & Martínez, S. T. (2005). Inducción de dos enzimas pectolíticas en el modelo Fusarium oxysporum f. sp. dianthi - clavel. Revista Colombiana de Química, 34(1), 25–34. http://www.redalyc.org/articulo.oa?id=309026662003 Goodwin, P. H., Trueman, C., Loewen, S. A., & Tazhoor, R. (2018). Variation in the responsiveness of induced resistance against Pseudomonas syringae pv. tomato by Solanum lycopersicum treated with para-aminobenzoic acid. Physiological and Molecular Plant Pathology, 104, 31–39. https://doi.org/10.1016/J.PMPP .2018.08.007 Gullino, M. L., Daughtrey, M. L., Garibaldi, A., & Elmer, W. H. (2015). Fusarium wilts of ornamental crops and their management. Crop Protection, 73, 50–59. https://doi.org/https://doi.org/10.1016/j.cropro.2015.01.003 Hallen-Adams, H. E., Wenner, N., Kuldau, G. A., & Trail, F. (2011). Deoxynivalenol Biosynthesis-Related Gene Expression During Wheat Kernel Colonization by Fusarium graminearum. Phytopathology®, 101(9), 1091–1096. https://doi.org/10.1094/PHYTO-01-11-0023 Harbaugh, D. T., Nepokroeff, M., Rabeler, R. K., McNeill, J., Zimmer, E. A., & Wagner, W. L. (2010). A New Lineage-Based Tribal Classification of the Family Caryophyllaceae. 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Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ardila Barrantes, Harold Duban3c0b4dc91cdf02792559faddcd37e2b3Castiblanco Quiroga, Nelly Fernandad206bc3c8a0a1c7af1e5fa44e3018d1fEstudio de Actividades Metabólicas Vegetales2023-06-14T23:13:05Z2023-06-14T23:13:05Z2023-06-15https://repositorio.unal.edu.co/handle/unal/84021Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías a colorLa tiamina se ha postulado como un potencial inductor de resistencia al marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi (Fod), dado que su aspersión foliar en plantas de clavel reduce significativamente la severidad de la enfermedad. No obstante, hasta el momento se desconocen los fenómenos bioquímicos involucrados en la inducción de resistencia en este modelo, por ejemplo, no se conoce el efecto que puede tener este tipo de sustancias directamente sobre el patógeno. En este estudio se investigó el efecto que tiene la tiamina sobre el crecimiento micelial y los niveles transcripcionales de los genes sod1, sed4, six7 y six10 los cuales codifican para posibles factores de virulencia de este patógeno del clavel. Para ello, primero se realizó el cultivo in vitro del patógeno y se determinó el efecto de la presencia de tiamina sobre el crecimiento y los niveles de transcripción de sod1 y sed4. Posteriormente, se inocularon con Fod plantas de clavel previamente tratadas con tiamina y se confirmó el efecto de ésta en la disminución de la severidad a la enfermedad; en este punto se evaluaron los niveles de transcripción in planta de los genes sed4, sod1, six7 y six10 en dos variedades de clavel con niveles de resistencia contrastante al marchitamiento vascular. En la primera etapa, se evidenció que, durante la fase exponencial, la presencia de tiamina genera una disminución de hasta un 68% en el crecimiento micelial, al comparar con el control; así mismo se observó que la presencia de este compuesto genera una modulación de los niveles transcripcionales de sod1 y sed4 en función de la fase de crecimiento del patógeno. A nivel in planta, se confirmó que la aspersión foliar de tiamina disminuye la severidad de la enfermedad causada por Fod y que, durante este proceso, hay una disminución de los niveles de expresión de sod1, six7, six10 y un aumento de la expresión de sed4. Además, se observó que los niveles transcripcionales de estos genes se encuentran modulados diferencialmente en función del nivel de resistencia de la planta. Estos hallazgos sugieren que la tiamina actúa como potencial inhibidor del crecimiento micelial in vitro y como un regulador de la transcripción de diversos genes relacionados con virulencia en Fusarium oxysporum f. sp. dianthi. (Texto tomado de la fuente)Thiamine has been proposed as a potential inducer of resistance to vascular wilt caused by Fusarium oxysporum f. sp. dianthi (Fod), since its foliar spraying on carnation plants results in a significant reduction in the severity of the disease. However, the biochemical phenomena involved in the induction of resistance in this model are still unknown: for example, the effect that this type of substance can have directly on the pathogen is also unknown. In this study, we investigate the effect of thiamine on mycelial growth and transcriptional levels of the sod1, sed4, six7 and six10 genes, which are encoding potential virulence factors of this carnation pathogen. For that purpose, the pathogen was first cultured in vitro and the effect of the presence of thiamine on the growth and transcriptional levels of sod1 and sed4 was evaluated. Subsequently, carnation plants previously treated with thiamine were inoculated with Fod and the effect of thiamine on the reduction of disease severity was confirmed; at this point, the in planta transcription levels of the sed4, sod1, six7 and six10 genes were analyzed in two carnation varieties with contrasting levels of resistance to vascular wilt. In the first stage, it was found that, during the exponential phase, the presence of thiamine generated a decrease of up to 68% in mycelial growth, when compared to the control; it was also observed that the presence of this compound generated a modulation of the transcriptional levels of sod1 and sed4 depending on the growth phase of the pathogen. At the in planta level, it was confirmed that the foliar spray of thiamine reduces the severity of the disease caused by Fod and that during this process, there is a decrease in the expression levels of sod1, six7, six10 and an increase in the expression of sed4. Furthermore, the transcriptional levels of these genes were found to be differentially modulated depending on the level of plant resistance. These findings suggested that thiamine acts as a potential inhibitor of mycelial growth in vitro and as a transcriptional regulator of several virulence-related genes in Fusarium oxysporum f. sp. dianthi.MaestríaInteracción Hospedero - Patógenoxxiii,110 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá,ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - Bioquímica580 - Plantas540 - Química y ciencias afines580 - Plantas::582 - Plantas destacadas por características vegetativas y floresClavelesVirus fitopatógenosVirosis (plantas)CarnationsPlant virusesVirus diseases of plantsFusarium oxysporum f. sp. dianthiDianthus caryophyllus L.TiaminaSuperóxido dismutasaTripeptidil peptidasaProteínas SIXFactor de virulenciaFusarium oxysporum f. sp. dianthiDianthus caryophyllus L.ThiamineSuperoxide dismutaseTripeptidyl peptidaseSIX proteinsVirulence factorEfecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthiEffect of the application of resistance inducers on the in vivo expression of candidate virulence factor genes of the pathogen Fusarium oxysporum f. sp. dianthi in carnation (Dianthus caryophyllus L)Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbdel-Monaim, M. 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Molecular Plant Pathology, 22(7), 882–895. https://doi.org/https://doi.org/10.1111/mpp.13068MinCienciasPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84021/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53ORIGINAL1012406246.2023.pdf1012406246.2023.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf4828152https://repositorio.unal.edu.co/bitstream/unal/84021/4/1012406246.2023.pdf01dae7f037a94fc326a8d97fb3e0afa7MD54THUMBNAIL1012406246.2023.pdf.jpg1012406246.2023.pdf.jpgGenerated Thumbnailimage/jpeg5788https://repositorio.unal.edu.co/bitstream/unal/84021/5/1012406246.2023.pdf.jpg6a145515fb85a0c0d32fe934a0b4c3e8MD55unal/84021oai:repositorio.unal.edu.co:unal/840212024-08-06 23:10:48.688Repositorio Institucional Universidad Nacional de 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