Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos

ilustraciones, fotografías, gráficas, tablas

Autores:
Ramírez Yépez, Camilo Sebastián
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/80922
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/80922
https://repositorio.unal.edu.co/
Palabra clave:
610 - Medicina y salud::611 - Anatomía humana, citología, histología
Modelos Animales
Condrogénesis
Células Madre
Models, Animal
Chondrogenesis
Stem Cells
Cartílago
Células madre mesenquimales
Medios condicionados
Secretoma
Cartilage
Mesenchimal stem cells
Conditioned media
Secretome
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_0f5b597701dc79e788d61e7f7c99362e
oai_identifier_str oai:repositorio.unal.edu.co:unal/80922
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
dc.title.translated.eng.fl_str_mv Evaluation of adipose derived mesenchymal stem cell conditioned media mediated chondrogenesis versus platelet rich plasma in knee osteochondral defects: an experimental model in rabbits
title Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
spellingShingle Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
610 - Medicina y salud::611 - Anatomía humana, citología, histología
Modelos Animales
Condrogénesis
Células Madre
Models, Animal
Chondrogenesis
Stem Cells
Cartílago
Células madre mesenquimales
Medios condicionados
Secretoma
Cartilage
Mesenchimal stem cells
Conditioned media
Secretome
title_short Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
title_full Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
title_fullStr Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
title_full_unstemmed Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
title_sort Evaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejos
dc.creator.fl_str_mv Ramírez Yépez, Camilo Sebastián
dc.contributor.advisor.spa.fl_str_mv Ortiz Morales, Jorge Rolando
Chaparro Garzón, Orlando
Linero Segrera, Itali Marcelly
dc.contributor.author.spa.fl_str_mv Ramírez Yépez, Camilo Sebastián
dc.contributor.researchgroup.spa.fl_str_mv Biología de Células Madre
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud::611 - Anatomía humana, citología, histología
topic 610 - Medicina y salud::611 - Anatomía humana, citología, histología
Modelos Animales
Condrogénesis
Células Madre
Models, Animal
Chondrogenesis
Stem Cells
Cartílago
Células madre mesenquimales
Medios condicionados
Secretoma
Cartilage
Mesenchimal stem cells
Conditioned media
Secretome
dc.subject.decs.spa.fl_str_mv Modelos Animales
Condrogénesis
Células Madre
dc.subject.decs.eng.fl_str_mv Models, Animal
Chondrogenesis
Stem Cells
dc.subject.proposal.spa.fl_str_mv Cartílago
Células madre mesenquimales
Medios condicionados
Secretoma
dc.subject.proposal.eng.fl_str_mv Cartilage
Mesenchimal stem cells
Conditioned media
Secretome
description ilustraciones, fotografías, gráficas, tablas
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-02-09T17:27:29Z
dc.date.available.none.fl_str_mv 2022-02-09T17:27:29Z
dc.date.issued.none.fl_str_mv 2022
dc.type.spa.fl_str_mv Trabajo de grado - Especialidad Médica
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/80922
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/80922
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv Bireme
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dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ortiz Morales, Jorge Rolandoa1e3598a562d115367611270c313f2e9600Chaparro Garzón, Orlando01a9c792304abaab8fb28a2e998aa841Linero Segrera, Itali Marcellyf35f8de0ed1e0a9d2d6f143f303afff0600Ramírez Yépez, Camilo Sebastiánb6b244d70e7c191b3b0f9e5f6b2ac339Biología de Células Madre2022-02-09T17:27:29Z2022-02-09T17:27:29Z2022https://repositorio.unal.edu.co/handle/unal/80922Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficas, tablasIntroducción: Las lesiones del cartílago hialino, ya sean de tipo traumático o degenerativo dan lugar a defectos condrales u osteocondrales que no cicatrizan con cartílago hialino nuevo. Por esta razón se ha investigado en la terapia regenerativa, con especial atención al secretoma de las células madre mesenquimales (MSC). En el presente estudio se plantea evaluar la condrogénesis del plasma rico en plaquetas (PRP) suplementado con medios condicionados de las células madre mesenquimales derivadas de tejido adiposo (AD-MSC-CM) en un modelo de defecto osteocondral (DO) de rodilla en conejos. Materiales y métodos: se plantea un estudio por fases, una fase piloto en la cual se establece el modelo experimental estandarizando primero la técnica quirúrgica con seis piezas cadavéricas, luego se estandariza el método de administración del tratamiento con dos conejos Nueva Zelanda con supervivencia. El primero se trata únicamente PRP administrado de forma infiltrativa en la rodilla, el segundo se trata con PRP + AD-MSC-CM. Se practica eutanasia a los 7 días y se evalúan los desenlaces y las complicaciones perioperatorias. En las fases siguientes del trabajo se plantea un estudio con 20 conejos: un grupo sham (n=2), un grupo control (n=6), un grupo tratado con PRP (n=6) y un grupo tratado con PRP + AD-MSC-CM (n=6), se sacrifican a las 12 semanas y se evalúa la regeneración condral de forma macroscópica e histológica; y la diferenciación del tipo de cartílago mediante inmunohistoquímica. Resultados: El presente documento muestra los resultados de la fase piloto determinando el diseño del modelo esperimental. Se propone un DO en el surco troclear al lado del tendón extensor digitorium longus. A los 7 días, ambos tratamientos seguían intraarticulares, sin embargo, mientras que el conejo tratado únicamente con PRP no mostraba ningún cambio en el DO, el conejo tratado con PRP + AD-MSC-CM exhibía características de cicatrización en el defecto creado rellenando parcialmente el mismo. Durante siete días, ninguno de los conejos mostró ningún tipo de efecto adverso más que una leve cojera. Tampoco mostraron signos importantes de dolor, malestar o angustia por lo que no hubo necesidad de aplicar criterios de punto final. Discusión y conclusiones: A pesar de las diferencias entre especies, el planteamiento de un DO en conejos permite evaluar el principio del tratamiento a aplicar siendo un modelo costo efectivo, que permitirá analizar la capacidad condrogénica del PRP y los AD-MCS-CM. El PRP es una buena estrategia que sirve como andamiaje para la entrega de factores de crecimiento y además actúa de forma sinérgica con los AD-MSC-CM. Debemos aclarar que los resultados respecto a la condrogénesis deben analizarse mediante un estudio histopatológico en las siguientes fases del estudio y tras haber pasado 12 semanas. A pesar de haber realizado un implante xenogénico no observamos ningún tipo de evento adverso en el conejo tratado con AD-MSC-CM, posiblemente indica que estos son seguros en la aplicación en estudios ulteriores. (Texto tomado de la fuente).Evaluation of adipose-derived mesenchymal stem cell conditioned media mediated chondrogenesis versus platelet-rich plasma in knee osteochondral defects: an experimental model in rabbits Introduction: Hyaline cartilage injuries, whether traumatic or degenerative, give rise to chondral or osteochondral defects that do not heal with new hyaline cartilage. For this reason, it has been investigated in regenerative therapy, with special attention to the mesenchymal stem cell (MSC) secretome. The present study aims to evaluate the chondrogenesis of platelet-rich plasma (PRP) supplemented with adipose-derived mesenchymal stem cells conditioned media (AD-MSC-CM) in a model of knee osteochondral defect (OD) in rabbits. Materials y methods: A phased study is proposed, a pilot phase in which the experimental model is established, first standardizing the surgical technique with six cadaveric pieces, then the treatment administration method is standardized with two New Zealand rabbits with survival. The first is treated only with PRP administered infiltratively in the knee, the second is treated with PRP + AD-MSC-CM. Euthanasia is performed at 7 days and perioperative outcomes and complications are evaluated. In the following phases of the work, a study with 20 rabbits is proposed: a sham group (n=2), a control group (n=6), a group treated with PRP (n=6, and a group treated with PRP + AD. -MSC-CM (n=6), sacrificed at 12 weeks and chondral regeneration is evaluated macroscopically and histologically; and differentiation of cartilage type by immunohistochemistry. Results: This document shows the results of the pilot phase determining the design of the experimental model. A DO is proposed in the trochlear groove next to the extensor digitorium longus tendon. At 7 days, both treatments were still intra-articular, however, while the rabbit treated with PRP alone did not show any change in DO, the rabbit treated with PRP + AD-MSC-CM exhibited healing characteristics in the defect created by partially filling the same. For seven days, none of the rabbits showed any adverse effects other than a slight lameness. They also did not show significant signs of pain, discomfort or distress, so there was no need to apply endpoint criteria. Discussion and conclusions: Despite the differences between species, the approach of a DO in rabbits allows evaluating the principle of the treatment to be applied, being a cost-effective model, which will allow analyzing the chondrogenic capacity of PRP and AD-MCS-CM. PRP is a good strategy that serves as a scaffold for the delivery of growth factors and also acts synergistically with AD-MSC-CM. We must clarify that the results regarding chondrogenesis must be analyzed by means of a histopathological study in the following phases of the study and after 12 weeks have passed. Despite having performed a xenogenic implant, we did not observe any type of adverse event in the rabbit treated with AD-MSC-CM, possibly indicating that these are safe in the application in further studies.Incluye anexosEspecialidades MédicasEspecialista en Ortopedia y TraumatologíaEstudio experimental en un modelo animalCirugía de rodillaTecnología de tejidosCiencias básicasxiii, 71 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Especialidad en Ortopedia y TraumatologíaDepartamento de CirugíaFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::611 - Anatomía humana, citología, histologíaModelos AnimalesCondrogénesisCélulas MadreModels, AnimalChondrogenesisStem CellsCartílagoCélulas madre mesenquimalesMedios condicionadosSecretomaCartilageMesenchimal stem cellsConditioned mediaSecretomeEvaluación de la condrogénesis mediada por medios condicionados de células madre mesenquimales derivadas de tejido adiposo versus plasma rico en plaquetas en defectos osteocondrales de rodilla: un modelo experimental en conejosEvaluation of adipose derived mesenchymal stem cell conditioned media mediated chondrogenesis versus platelet rich plasma in knee osteochondral defects: an experimental model in rabbitsTrabajo de grado - Especialidad Médicainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBireme1. 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Journal of Orthopaedic Research. 2010 Aug;28(8):1064–70.Convocatoria para el Apoyo a Proyectos de Investigación, Creación Artística e Innovación de la Sede de Bogota de la universidad Nacional de Colombia – 2020 (HERMES)EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/80922/2/license.txt8153f7789df02f0a4c9e079953658ab2MD52ORIGINAL1030594330.2022.pdf1030594330.2022.pdfTesis de Especialidad en Ortopedia y Traumatologíaapplication/pdf1717708https://repositorio.unal.edu.co/bitstream/unal/80922/1/1030594330.2022.pdfe7047ca5a8aa7d5303597d8c2f463ea6MD51THUMBNAIL1030594330.2022.pdf.jpg1030594330.2022.pdf.jpgGenerated Thumbnailimage/jpeg5029https://repositorio.unal.edu.co/bitstream/unal/80922/3/1030594330.2022.pdf.jpg39cfa944c6065bfc36cc88d6a00a8059MD53unal/80922oai:repositorio.unal.edu.co:unal/809222024-08-02 23:11:11.69Repositorio Institucional Universidad Nacional de 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EVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLiAqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4gCgpBbCBoYWNlciBjbGljIGVuIGVsIHNpZ3VpZW50ZSBib3TDs24sIHVzdGVkIGluZGljYSBxdWUgZXN0w6EgZGUgYWN1ZXJkbyBjb24gZXN0b3MgdMOpcm1pbm9zLiBTaSB0aWVuZSBhbGd1bmEgZHVkYSBzb2JyZSBsYSBsaWNlbmNpYSwgcG9yIGZhdm9yLCBjb250YWN0ZSBjb24gZWwgYWRtaW5pc3RyYWRvciBkZWwgc2lzdGVtYS4KClVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIC0gw5psdGltYSBtb2RpZmljYWNpw7NuIDE5LzEwLzIwMjEK

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