Effect of pH on the rheological response of reconstituted gastric mucin
The rheological response of reconstituted gastric mucin was studied by time dependent rheological experiments as function of pH. Mucin concentrations of 5% and 10% were prepared in buffer dispersions at pH of 1.15, 2.00, 2.55, 4.00 and 7.67. The isoelectric point was identified by z-potential betwe...
- Autores:
-
Caicedo, Jonathan Argenis
Perilla, Jairo Ernesto
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/67678
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/67678
http://bdigital.unal.edu.co/68707/
- Palabra clave:
- 62 Ingeniería y operaciones afines / Engineering
Mucin
rheology
z-potential
creep
elasticity
Mucina
reología
potencial zeta
fluencia lenta
elasticidad.
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Effect of pH on the rheological response of reconstituted gastric mucin |
| title |
Effect of pH on the rheological response of reconstituted gastric mucin |
| spellingShingle |
Effect of pH on the rheological response of reconstituted gastric mucin 62 Ingeniería y operaciones afines / Engineering Mucin rheology z-potential creep elasticity Mucina reología potencial zeta fluencia lenta elasticidad. |
| title_short |
Effect of pH on the rheological response of reconstituted gastric mucin |
| title_full |
Effect of pH on the rheological response of reconstituted gastric mucin |
| title_fullStr |
Effect of pH on the rheological response of reconstituted gastric mucin |
| title_full_unstemmed |
Effect of pH on the rheological response of reconstituted gastric mucin |
| title_sort |
Effect of pH on the rheological response of reconstituted gastric mucin |
| dc.creator.fl_str_mv |
Caicedo, Jonathan Argenis Perilla, Jairo Ernesto |
| dc.contributor.author.spa.fl_str_mv |
Caicedo, Jonathan Argenis Perilla, Jairo Ernesto |
| dc.subject.ddc.spa.fl_str_mv |
62 Ingeniería y operaciones afines / Engineering |
| topic |
62 Ingeniería y operaciones afines / Engineering Mucin rheology z-potential creep elasticity Mucina reología potencial zeta fluencia lenta elasticidad. |
| dc.subject.proposal.spa.fl_str_mv |
Mucin rheology z-potential creep elasticity Mucina reología potencial zeta fluencia lenta elasticidad. |
| description |
The rheological response of reconstituted gastric mucin was studied by time dependent rheological experiments as function of pH. Mucin concentrations of 5% and 10% were prepared in buffer dispersions at pH of 1.15, 2.00, 2.55, 4.00 and 7.67. The isoelectric point was identified by z-potential between pH 2.00 to 2.55. Dynamic light scattering showed that as pH reduced, a second population of larger mucin aggregates was formed indicating the presence of new structures. Steady shear rheological measurements reflected the pseudoplastic behavior of mucin dispersions and the effect of concentration on viscosity. Creep-recovery measurements were performed on the regenerated mucus at different levels of pH. By creep, it was possible to determine the values of zero-shear-viscosity of mucin suspensions, with higher precision and in a lower experimental time than steady shear measurements. Additionally, it was found that at pH ~1.15, the viscosity of the mucus increased to high values, which is an indicative of a gel-like structure. By recovery experiments, it was possible to find that even the very low viscosities the mucin suspensions at pH ~1.15 possessed a defined elastic character. By the use of a four-element mechanistic viscoelastic model, it was concluded that this elasticity underwent retardation due to the combined effect of viscous and elastic responses. |
| publishDate |
2015 |
| dc.date.issued.spa.fl_str_mv |
2015-05-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-03T04:50:20Z |
| dc.date.available.spa.fl_str_mv |
2019-07-03T04:50:20Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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publishedVersion |
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ISSN: 2248-8723 |
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https://repositorio.unal.edu.co/handle/unal/67678 |
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http://bdigital.unal.edu.co/68707/ |
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ISSN: 2248-8723 |
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https://repositorio.unal.edu.co/handle/unal/67678 http://bdigital.unal.edu.co/68707/ |
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spa |
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spa |
| dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/ingeinv/article/view/50019 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Ingeniería e Investigación Ingeniería e Investigación |
| dc.relation.references.spa.fl_str_mv |
Caicedo, Jonathan Argenis and Perilla, Jairo Ernesto (2015) Effect of pH on the rheological response of reconstituted gastric mucin. Ingeniería e Investigación, 35 (2). pp. 43-48. ISSN 2248-8723 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ingeniería |
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Caicedo, Jonathan Argenisf6add073-51c1-4103-bc6e-a47eede9a25c300Perilla, Jairo Ernestoa2677a8a-0178-401a-81ae-66ff9e097ceb3002019-07-03T04:50:20Z2019-07-03T04:50:20Z2015-05-01ISSN: 2248-8723https://repositorio.unal.edu.co/handle/unal/67678http://bdigital.unal.edu.co/68707/The rheological response of reconstituted gastric mucin was studied by time dependent rheological experiments as function of pH. Mucin concentrations of 5% and 10% were prepared in buffer dispersions at pH of 1.15, 2.00, 2.55, 4.00 and 7.67. The isoelectric point was identified by z-potential between pH 2.00 to 2.55. Dynamic light scattering showed that as pH reduced, a second population of larger mucin aggregates was formed indicating the presence of new structures. Steady shear rheological measurements reflected the pseudoplastic behavior of mucin dispersions and the effect of concentration on viscosity. Creep-recovery measurements were performed on the regenerated mucus at different levels of pH. By creep, it was possible to determine the values of zero-shear-viscosity of mucin suspensions, with higher precision and in a lower experimental time than steady shear measurements. Additionally, it was found that at pH ~1.15, the viscosity of the mucus increased to high values, which is an indicative of a gel-like structure. By recovery experiments, it was possible to find that even the very low viscosities the mucin suspensions at pH ~1.15 possessed a defined elastic character. By the use of a four-element mechanistic viscoelastic model, it was concluded that this elasticity underwent retardation due to the combined effect of viscous and elastic responses.Se estudió la respuesta reológica de mucina gástrica reconstituida como función del pH mediante experimentos reológicos dependientes del tiempo. Se usaron concentraciones de mucina del 5% y 10% en soluciones buffer a valores de pH de 1.15, 2.00, 2.55, 4.00 y 7.67. Mediante potencial zeta se identifico que el punto isoeléctrico se ubicaba entre pH de 2.00 a 2.55. Los estudios de dispersión de luz dinámica mostraron que a mediada que el pH se reduce, una segunda población de agregados de mucina se forma, indicando el estructuramiento dentro del fluido. Las mediciones reológicas en estado estable, mostraron el comportamiento seudoplástico y el efecto de la concentración en las soluciones de mucina. Se llevaron a cabo determinaciones de fluencia lenta-recuperación sobre las suspensiones de mucina a diferentes valores de pH. Mediante fluencia lenta, se determinaron los valores de viscosidad a cero corte con mayor precisión y menor tiempo experimental que las mediciones de estado estable. Adicionalmente, se encontró que para pH ~1.15 la viscosidad del mucus incrementó a valores altos, lo cual es una característica de las estructuras tipo gel. Los experimentos de recuperación mostraron que aun a bajas viscosidades las suspensiones de mucina a pH ~1.15 poseían un carácter elástico definido. Mediante el uso de un modelo viscoelástico de cuatro elementos, se concluyó que esta elasticidad presentaba respuesta retardada debido al efecto combinado de los efectos viscoso y elástico.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ingenieríahttps://revistas.unal.edu.co/index.php/ingeinv/article/view/50019Universidad Nacional de Colombia Revistas electrónicas UN Ingeniería e InvestigaciónIngeniería e InvestigaciónCaicedo, Jonathan Argenis and Perilla, Jairo Ernesto (2015) Effect of pH on the rheological response of reconstituted gastric mucin. Ingeniería e Investigación, 35 (2). pp. 43-48. ISSN 2248-872362 Ingeniería y operaciones afines / EngineeringMucinrheologyz-potentialcreepelasticityMucinareologíapotencial zetafluencia lentaelasticidad.Effect of pH on the rheological response of reconstituted gastric mucinArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL50019-255976-4-PB.pdfapplication/pdf539550https://repositorio.unal.edu.co/bitstream/unal/67678/1/50019-255976-4-PB.pdffb60f606118daac62b6b472d348fc7e5MD51THUMBNAIL50019-255976-4-PB.pdf.jpg50019-255976-4-PB.pdf.jpgGenerated Thumbnailimage/jpeg8334https://repositorio.unal.edu.co/bitstream/unal/67678/2/50019-255976-4-PB.pdf.jpgea6b1ac7bd5287bd6770205659a40948MD52unal/67678oai:repositorio.unal.edu.co:unal/676782023-05-31 23:03:07.418Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |