Molecular tools for mycobacterium tuberculosis genotyping

Objective The present work studied molecular typing methods used for Mycobacterium tuberculosis characterization in order to learn about their advantages, disadvantages and discrimination power as regards the implementation of tuberculosis surveillance and control programs. Methods To analyze the di...

Full description

Autores:: Rozo-Anaya, Juan C.
Ribón, Wellman

Tipo de recurso:: Article of journal

Fecha de publicación:: 2010

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

id	UNACIONAL2_0d1ddbac140261713a3ac56794bcf569
oai_identifier_str	oai:repositorio.unal.edu.co:unal/35257
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
spelling	Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rozo-Anaya, Juan C.b50a530d-e225-424b-ad26-fd0c1b4ba7eb300Ribón, Wellman73541a13-d0e1-4d1c-8b76-1bf809ce155c3002019-06-28T00:04:52Z2019-06-28T00:04:52Z2010https://repositorio.unal.edu.co/handle/unal/35257http://bdigital.unal.edu.co/25337/Objective The present work studied molecular typing methods used for Mycobacterium tuberculosis characterization in order to learn about their advantages, disadvantages and discrimination power as regards the implementation of tuberculosis surveillance and control programs. Methods To analyze the discrimination power of each method we studied articles that included Hunter-Gaston discrimination index (HGDI) values or data allowing their calculation. Results The highest discrimination power was registered for LM-PCR followed by FLiP and 15-loci MIRU. The most frequently used methods showed an HGDI of 0.9491, 0.9519 and 0.8630 for 12-loci MIRU, RFLP-IS6110 and spoligotyping, respectively. Conclusion M. tuberculosis isolates molecular characterization requires at least two molecular markers to discriminate non related isolates, as well as previous analysis to their implementation.Objetivo En el presente trabajo se estudiaron las metodologías de tipificación molecular empleadas para caracterizar Mycobacterium tuberculosis con el objetivo de conocer las ventajas, desventajas y poder discriminatorio para ser consideradas al momento de la implementación en los programas de vigilancia y control de la tuberculosis. Métodos Para el análisis del poder discriminatorio de cada metodología se estudiaron los artículos que suministraban el valor del Hunter-Gaston discrimination index (HGDI) ó los datos que permitían su determinación. Resultados Se documentó que el LM-PCR tiene una mayor capacidad discriminatoria seguida de FLiP y MIRU de 15 loci. Las metodologías más comúnmente empleadas mostraron un HGDI de 0.9491, 0.9519 y 0.8630 para MIRU de 12 loci, RFLP-IS6110 y spoligotyping respectivamente. Conclusión La caracterización molecular de aislamientos de M. tuberculosis requiere mínimo el análisis de al menos dos marcadores moleculares para discriminar aislamientos no relacionados y la necesidad de realizar análisis previos a la implementación de estas metodologías.application/pdfspaRevista de Salud Públicahttp://revistas.unal.edu.co/index.php/revsaludpublica/article/view/22684Universidad Nacional de Colombia Revistas electrónicas UN Revista de Salud PúblicaRevista de Salud PúblicaJournal of Public Health; Vol. 12, núm. 3 (2010); 510-521 Revista de Salud Pública; Vol. 12, núm. 3 (2010); 510-521 0124-0064Rozo-Anaya, Juan C. and Ribón, Wellman (2010) Molecular tools for mycobacterium tuberculosis genotyping. Journal of Public Health; Vol. 12, núm. 3 (2010); 510-521 Revista de Salud Pública; Vol. 12, núm. 3 (2010); 510-521 0124-0064 .Molecular tools for mycobacterium tuberculosis genotypingArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTTuberculosisMycobacterium tuberculosisepidemiologia moleculargenotipoADNpolimorfismo de longitud del fragmento de restricciónTuberculosisMycobacterium tuberculosismolecular epidemiologygenotypeDNAPolymorphismRestriction Fragment LengthORIGINAL22684-78390-1-PB.pdfapplication/pdf22229448https://repositorio.unal.edu.co/bitstream/unal/35257/1/22684-78390-1-PB.pdf1bd9baa1d587339880b97be07734bb4bMD51THUMBNAIL22684-78390-1-PB.pdf.jpg22684-78390-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg5503https://repositorio.unal.edu.co/bitstream/unal/35257/2/22684-78390-1-PB.pdf.jpgf2fa3d2876bbd7e9a24ace40e7727875MD52unal/35257oai:repositorio.unal.edu.co:unal/352572023-01-04 23:03:27.711Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv	Molecular tools for mycobacterium tuberculosis genotyping
title	Molecular tools for mycobacterium tuberculosis genotyping
spellingShingle	Molecular tools for mycobacterium tuberculosis genotyping Tuberculosis Mycobacterium tuberculosis epidemiologia molecular genotipo ADN polimorfismo de longitud del fragmento de restricción Tuberculosis Mycobacterium tuberculosis molecular epidemiology genotype DNA Polymorphism Restriction Fragment Length
title_short	Molecular tools for mycobacterium tuberculosis genotyping
title_full	Molecular tools for mycobacterium tuberculosis genotyping
title_fullStr	Molecular tools for mycobacterium tuberculosis genotyping
title_full_unstemmed	Molecular tools for mycobacterium tuberculosis genotyping
title_sort	Molecular tools for mycobacterium tuberculosis genotyping
dc.creator.fl_str_mv	Rozo-Anaya, Juan C. Ribón, Wellman
dc.contributor.author.spa.fl_str_mv	Rozo-Anaya, Juan C. Ribón, Wellman
dc.subject.proposal.spa.fl_str_mv	Tuberculosis Mycobacterium tuberculosis epidemiologia molecular genotipo ADN polimorfismo de longitud del fragmento de restricción Tuberculosis Mycobacterium tuberculosis molecular epidemiology genotype DNA Polymorphism Restriction Fragment Length
topic	Tuberculosis Mycobacterium tuberculosis epidemiologia molecular genotipo ADN polimorfismo de longitud del fragmento de restricción Tuberculosis Mycobacterium tuberculosis molecular epidemiology genotype DNA Polymorphism Restriction Fragment Length
description	Objective The present work studied molecular typing methods used for Mycobacterium tuberculosis characterization in order to learn about their advantages, disadvantages and discrimination power as regards the implementation of tuberculosis surveillance and control programs. Methods To analyze the discrimination power of each method we studied articles that included Hunter-Gaston discrimination index (HGDI) values or data allowing their calculation. Results The highest discrimination power was registered for LM-PCR followed by FLiP and 15-loci MIRU. The most frequently used methods showed an HGDI of 0.9491, 0.9519 and 0.8630 for 12-loci MIRU, RFLP-IS6110 and spoligotyping, respectively. Conclusion M. tuberculosis isolates molecular characterization requires at least two molecular markers to discriminate non related isolates, as well as previous analysis to their implementation.
publishDate	2010
dc.date.issued.spa.fl_str_mv	2010
dc.date.accessioned.spa.fl_str_mv	2019-06-28T00:04:52Z
dc.date.available.spa.fl_str_mv	2019-06-28T00:04:52Z
dc.type.spa.fl_str_mv	Artículo de revista
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.spa.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/ART
format	http://purl.org/coar/resource_type/c_6501
status_str	publishedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/35257
dc.identifier.eprints.spa.fl_str_mv	http://bdigital.unal.edu.co/25337/
url	https://repositorio.unal.edu.co/handle/unal/35257 http://bdigital.unal.edu.co/25337/
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.spa.fl_str_mv	http://revistas.unal.edu.co/index.php/revsaludpublica/article/view/22684
dc.relation.ispartof.spa.fl_str_mv	Universidad Nacional de Colombia Revistas electrónicas UN Revista de Salud Pública Revista de Salud Pública
dc.relation.ispartofseries.none.fl_str_mv	Journal of Public Health; Vol. 12, núm. 3 (2010); 510-521 Revista de Salud Pública; Vol. 12, núm. 3 (2010); 510-521 0124-0064
dc.relation.references.spa.fl_str_mv	Rozo-Anaya, Juan C. and Ribón, Wellman (2010) Molecular tools for mycobacterium tuberculosis genotyping. Journal of Public Health; Vol. 12, núm. 3 (2010); 510-521 Revista de Salud Pública; Vol. 12, núm. 3 (2010); 510-521 0124-0064 .
dc.rights.spa.fl_str_mv	Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv	Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv	http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.mimetype.spa.fl_str_mv	application/pdf
dc.publisher.spa.fl_str_mv	Revista de Salud Pública
institution	Universidad Nacional de Colombia
bitstream.url.fl_str_mv	https://repositorio.unal.edu.co/bitstream/unal/35257/1/22684-78390-1-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/35257/2/22684-78390-1-PB.pdf.jpg
bitstream.checksum.fl_str_mv	1bd9baa1d587339880b97be07734bb4b f2fa3d2876bbd7e9a24ace40e7727875
bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5
repository.name.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv	repositorio_nal@unal.edu.co
_version_	1814089713225564160

Molecular tools for mycobacterium tuberculosis genotyping

Publicaciones similares