Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate
Nitrogen concentration is an essential parameter in cyanobacterial cultures to produce enriched biomass with biotechnological purposes. Growth and biochemical composition of Nostoc LAUN0015, Nostoc UAM206, Anabaena sp.1 and Anabaena sp.2 were compared at 0, 4.25, 8.5 and 17 mM NaNO3. Cultures under...
- Autores:
-
Rosales Loaiza, Néstor
Vera, Patricia
Aiello-Mazzarri, Cateryna
Morales, Ever
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/61191
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/61191
http://bdigital.unal.edu.co/59999/
- Palabra clave:
- 57 Ciencias de la vida; Biología / Life sciences; biology
Anabaena
biochemical composition
biomass
culture
nitrate
Nostoc
biomasa
composición bioquímica
cultivo
nitrato.
cultivo
Anabaena
nitrato
Nostoc
biomasa
composición bioquímica
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
| id |
UNACIONAL2_0ca94b31dd7eeee3a2f77d91a3cb9cc3 |
|---|---|
| oai_identifier_str |
oai:repositorio.unal.edu.co:unal/61191 |
| network_acronym_str |
UNACIONAL2 |
| network_name_str |
Universidad Nacional de Colombia |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate |
| title |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate |
| spellingShingle |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate 57 Ciencias de la vida; Biología / Life sciences; biology Anabaena biochemical composition biomass culture nitrate Nostoc biomasa composición bioquímica cultivo nitrato. cultivo Anabaena nitrato Nostoc biomasa composición bioquímica |
| title_short |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate |
| title_full |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate |
| title_fullStr |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate |
| title_full_unstemmed |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate |
| title_sort |
Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate |
| dc.creator.fl_str_mv |
Rosales Loaiza, Néstor Vera, Patricia Aiello-Mazzarri, Cateryna Morales, Ever |
| dc.contributor.author.spa.fl_str_mv |
Rosales Loaiza, Néstor Vera, Patricia Aiello-Mazzarri, Cateryna Morales, Ever |
| dc.subject.ddc.spa.fl_str_mv |
57 Ciencias de la vida; Biología / Life sciences; biology |
| topic |
57 Ciencias de la vida; Biología / Life sciences; biology Anabaena biochemical composition biomass culture nitrate Nostoc biomasa composición bioquímica cultivo nitrato. cultivo Anabaena nitrato Nostoc biomasa composición bioquímica |
| dc.subject.proposal.spa.fl_str_mv |
Anabaena biochemical composition biomass culture nitrate Nostoc biomasa composición bioquímica cultivo nitrato. cultivo Anabaena nitrato Nostoc biomasa composición bioquímica |
| description |
Nitrogen concentration is an essential parameter in cyanobacterial cultures to produce enriched biomass with biotechnological purposes. Growth and biochemical composition of Nostoc LAUN0015, Nostoc UAM206, Anabaena sp.1 and Anabaena sp.2 were compared at 0, 4.25, 8.5 and 17 mM NaNO3. Cultures under laboratory conditions were maintained for 30 days at a volume of 500 mL. Anabaena sp.1 yielded the highest value of dry mass of 0.26 ± 2.49 mg mL-1 at 8.5 mM NaNO3. For chlorophyll, phycocyanin and phycoerythrin, maximum values were achieved at 17 mM NaNO3 with 18.09 ± 1.74, 102.90 ± 6.73 and 53.47 ± 2.40 μg mL-1, respectively. Nostoc LAUN0015 produced its maximum value of protein 644.86 ± 19.77 μg mL-1, and 890 mg mL-1 of carbohydrates in the absence of nitrogen. This comparative study shows that the most efficient strain for the production of protein, carbohydrates and lipids in diazotrophic conditions corresponded to Nostoc LAUN0015. However, Anabaena sp.1 and Anabaena sp.2 required high nitrogen concentrations to achieve higher values of metabolites, comparing with Nostoc strains. Nitrogen dependence for the production of pigments and high protein production in strains of Anabaena and in diazotrophic conditions for Nostoc was demonstrated. Nostoc can be cultured under nitrogen deficiency and Anabaena in sufficiency, for biomass production enriched with proteins and carbohydrates. |
| publishDate |
2016 |
| dc.date.issued.spa.fl_str_mv |
2016-05-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-02T20:09:34Z |
| dc.date.available.spa.fl_str_mv |
2019-07-02T20:09:34Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.issn.spa.fl_str_mv |
ISSN: 1900-1649 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/61191 |
| dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/59999/ |
| identifier_str_mv |
ISSN: 1900-1649 |
| url |
https://repositorio.unal.edu.co/handle/unal/61191 http://bdigital.unal.edu.co/59999/ |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/actabiol/article/view/48883 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica Colombiana Acta Biológica Colombiana |
| dc.relation.references.spa.fl_str_mv |
Rosales Loaiza, Néstor and Vera, Patricia and Aiello-Mazzarri, Cateryna and Morales, Ever (2016) Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate. Acta Biológica Colombiana, 21 (2). pp. 347-354. ISSN 1900-1649 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
| dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biología |
| institution |
Universidad Nacional de Colombia |
| bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/61191/1/48883-288422-1-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/61191/2/48883-288422-1-PB.pdf.jpg |
| bitstream.checksum.fl_str_mv |
33a8057a3792625a787433b32e156f06 dc2b5056b8a9ecb05a2b332df0602ad4 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| repository.mail.fl_str_mv |
repositorio_nal@unal.edu.co |
| _version_ |
1814089634613821440 |
| spelling |
Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rosales Loaiza, Néstor46d51e81-c621-4d14-94a0-356468fe816a300Vera, Patriciadaaa5c6d-7f2c-4bdd-a97b-7b37f4cae213300Aiello-Mazzarri, Cateryna21e854b9-0f49-4aa5-acef-1b352c117cd8300Morales, Ever34ba9927-206c-403e-844c-44d0f2e2ae763002019-07-02T20:09:34Z2019-07-02T20:09:34Z2016-05-01ISSN: 1900-1649https://repositorio.unal.edu.co/handle/unal/61191http://bdigital.unal.edu.co/59999/Nitrogen concentration is an essential parameter in cyanobacterial cultures to produce enriched biomass with biotechnological purposes. Growth and biochemical composition of Nostoc LAUN0015, Nostoc UAM206, Anabaena sp.1 and Anabaena sp.2 were compared at 0, 4.25, 8.5 and 17 mM NaNO3. Cultures under laboratory conditions were maintained for 30 days at a volume of 500 mL. Anabaena sp.1 yielded the highest value of dry mass of 0.26 ± 2.49 mg mL-1 at 8.5 mM NaNO3. For chlorophyll, phycocyanin and phycoerythrin, maximum values were achieved at 17 mM NaNO3 with 18.09 ± 1.74, 102.90 ± 6.73 and 53.47 ± 2.40 μg mL-1, respectively. Nostoc LAUN0015 produced its maximum value of protein 644.86 ± 19.77 μg mL-1, and 890 mg mL-1 of carbohydrates in the absence of nitrogen. This comparative study shows that the most efficient strain for the production of protein, carbohydrates and lipids in diazotrophic conditions corresponded to Nostoc LAUN0015. However, Anabaena sp.1 and Anabaena sp.2 required high nitrogen concentrations to achieve higher values of metabolites, comparing with Nostoc strains. Nitrogen dependence for the production of pigments and high protein production in strains of Anabaena and in diazotrophic conditions for Nostoc was demonstrated. Nostoc can be cultured under nitrogen deficiency and Anabaena in sufficiency, for biomass production enriched with proteins and carbohydrates.La concentración de nitrógeno constituye un parámetro esencial en cultivos de cianobacterias para la producción de biomasa enriquecida con fines biotecnológicos. Se comparó el crecimiento y composición bioquímica de las cepas Nostoc LAUN0015, Nostoc UAM206, Anabaena sp.1 y Anabaena sp.2 a 0, 4,25; 8,5 y 17 mM NaNO3. Los cultivos en condiciones de laboratorio fueron mantenidos durante 30 días a un volumen de 500 mL. En masa seca, Anabaena sp.1 obtuvo el mayor valor, con 2,49 ± 0,26 mg mL-1 a 8,5 mM NaNO3. Para clorofila, ficocianina y ficoeritrina, los máximos se alcanzaron a 17 mM NaNO3 en Anabaena sp.1, con 18,09 ± 1,74; 102,90 ± 6,73 y 53,47 ± 2,40 μg mL-1, respectivamente. Nostoc LAUN0015 produjo su máximo valor de proteínas de 644,86 ±19,77μg mL-1, y alrededor de 890 μg mL-1 de carbohidratos en ausencia de nitrógeno. El estudio comparativo indica que la cepa más eficiente para la producción de proteínas, carbohidratos y lípidos, en condiciones diazotróficas, correspondió a Nostoc LAUN0015. En cambio, las cepas de Anabaena sp.1 y sp.2 requieren de elevadas concentraciones de nitrógeno para alcanzar los mayores valores de metabolitos, respecto a las cepas de Nostoc. Se demuestra la dependencia de nitrógeno para la producción de los pigmentos y la alta producción proteica en las cepas de Anabaena y en condiciones diazotróficas para Nostoc. Esta última puede ser cultivada bajo una deficiencia de nitrógeno y Anabaena con suficiencia para la producción masiva de biomasa enriquecida con proteínas y carbohidratos.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biologíahttps://revistas.unal.edu.co/index.php/actabiol/article/view/48883Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica ColombianaActa Biológica ColombianaRosales Loaiza, Néstor and Vera, Patricia and Aiello-Mazzarri, Cateryna and Morales, Ever (2016) Comparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrate. Acta Biológica Colombiana, 21 (2). pp. 347-354. ISSN 1900-164957 Ciencias de la vida; Biología / Life sciences; biologyAnabaenabiochemical compositionbiomassculturenitrateNostocbiomasacomposición bioquímicacultivonitrato.cultivoAnabaenanitratoNostocbiomasacomposición bioquímicaComparative growth and biochemical composition of four strains of Nostoc and Anabaena (Cyanobacteria, Nostocales) in relation to sodium nitrateArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL48883-288422-1-PB.pdfapplication/pdf462392https://repositorio.unal.edu.co/bitstream/unal/61191/1/48883-288422-1-PB.pdf33a8057a3792625a787433b32e156f06MD51THUMBNAIL48883-288422-1-PB.pdf.jpg48883-288422-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg9964https://repositorio.unal.edu.co/bitstream/unal/61191/2/48883-288422-1-PB.pdf.jpgdc2b5056b8a9ecb05a2b332df0602ad4MD52unal/61191oai:repositorio.unal.edu.co:unal/611912023-04-10 23:05:57.86Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |