Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos

ilustraciones, fotografías, gráficas, tablas

Autores:
Villamil Bolaños, Fabian
Tipo de recurso:
Fecha de publicación:
2021
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/79642
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/79642
https://repositorio.unal.edu.co/
Palabra clave:
630 - Agricultura y tecnologías relacionadas
Transgenic plants
Biopolymers
Plantas transgénicas
Biopolímeros
Biopolímero
Transgénico
β-glucuronidasa
Peroxisoma
Péptido señal
Biopolymer
Transgenic
β-glucuronidase
Peroxisome
Signal peptide
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_0998c8055831c31fa76fcaeb98dba5aa
oai_identifier_str oai:repositorio.unal.edu.co:unal/79642
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
dc.title.translated.eng.fl_str_mv Transgenic line of Nicotiana tabacum expressing the phaC gene of Aeromonas caviae for the production of polyhydroxyalkanoates
title Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
spellingShingle Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
630 - Agricultura y tecnologías relacionadas
Transgenic plants
Biopolymers
Plantas transgénicas
Biopolímeros
Biopolímero
Transgénico
β-glucuronidasa
Peroxisoma
Péptido señal
Biopolymer
Transgenic
β-glucuronidase
Peroxisome
Signal peptide
title_short Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
title_full Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
title_fullStr Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
title_full_unstemmed Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
title_sort Línea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatos
dc.creator.fl_str_mv Villamil Bolaños, Fabian
dc.contributor.advisor.spa.fl_str_mv Sarmiento Salazar, Felipe
dc.contributor.author.spa.fl_str_mv Villamil Bolaños, Fabian
dc.contributor.researchgroup.spa.fl_str_mv Ingeniería Genética de Plantas
dc.subject.ddc.spa.fl_str_mv 630 - Agricultura y tecnologías relacionadas
topic 630 - Agricultura y tecnologías relacionadas
Transgenic plants
Biopolymers
Plantas transgénicas
Biopolímeros
Biopolímero
Transgénico
β-glucuronidasa
Peroxisoma
Péptido señal
Biopolymer
Transgenic
β-glucuronidase
Peroxisome
Signal peptide
dc.subject.agrovoc.eng.fl_str_mv Transgenic plants
Biopolymers
dc.subject.agrovoc.spa.fl_str_mv Plantas transgénicas
Biopolímeros
dc.subject.proposal.spa.fl_str_mv Biopolímero
Transgénico
β-glucuronidasa
Peroxisoma
Péptido señal
dc.subject.proposal.eng.fl_str_mv Biopolymer
Transgenic
β-glucuronidase
Peroxisome
Signal peptide
description ilustraciones, fotografías, gráficas, tablas
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-06-17T18:45:45Z
dc.date.available.none.fl_str_mv 2021-06-17T18:45:45Z
dc.date.issued.none.fl_str_mv 2021-07-11
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/79642
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/79642
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Sarmiento Salazar, Felipe97400f5ef687d1b92cbb7c10c002339cVillamil Bolaños, Fabiane0274278862f3e61bdadeae8b2b2e806Ingeniería Genética de Plantas2021-06-17T18:45:45Z2021-06-17T18:45:45Z2021-07-11https://repositorio.unal.edu.co/handle/unal/79642Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficas, tablasLos polihidroxialcanoatos (PHAs) son poliésteres producidos y degradados naturalmente por bacterias, cuyas propiedades los hacen similares a los plásticos derivados del petróleo. La producción en masa de PHAs es costosa, por ello la transferencia genética de genes clave y su producción en plantas se ha considerado como alternativa, dado que estos organismos tienen un bajo costo de mantenimiento y por qué pueden generar mayor biomasa. Sin embargo, uno de los problemas principales que han limitado su obtención, es que generalmente las plantas presentan problemas de desarrollo y crecimiento asociados al secuestro de sustancias claves para el metabolismo y dirigidas hacia la síntesis de PHAs. Hallazgos recientes han identificado que su biosíntesis en peroxisomas reduce los efectos negativos debido a la presencia y abundancia de compuestos intermediarios en la ruta de biosíntesis de estos biopolímeros. Por esta razón, nuestros objetivos se centraron en obtener líneas genéticamente modificadas de Nicotiana tabacum var. Samsun 10, transformadas mediante la infección con Agrobacterium tumefaciens cepa LBA4404 y en evaluar la expresión del casete que dirige la síntesis del gen phaCAC de Aeromonas caviae hacia peroxisomas. Los resultados de la extracción del ADN indicaron una eficiencia de transformación del 2,6%, la síntesis de ADNc y la evaluación de la actividad de la β-glucuronidasa, detectaron dos líneas transgénicas que expresaron el gen phaCAC sin efectos negativos aparentes. (Texto tomado de la fuente)Polyhydroxyalcanoates (PHAs) are polyesters naturally produced and degraded by bacteria, whose properties make them like plastics derived from petroleum. Mass production of PHAs by bacteria is expensive, so genetic transfer of key genes and production in plants has been considered as an alternative given the low maintenance cost and larger biomass than plants can generate. However, one of the main problems that have limited plant production is that plants generally present developmental and growth problems associated with capture of key substances for metabolism and directed towards PHA synthesis. Recent findings have identified that PHAs biosynthesis in peroxisomes reduces negative effects due to the presence and abundance of intermediate compounds in the biosynthesis path of these biopolymers. For this reason, our objectives focused on obtaining genetically modified lines of Nicotiana tabacum var. Samsun 10, transformed by infection with Agrobacterium tumefaciens strain LBA4404, and in to evaluate the expression of the construct that directs the synthesis of the phaCAC gene from Aeromonas caviae towards plant peroxisomes. DNA extraction results indicated 2.6% transformation efficiency and DNAc synthesis and evaluation of β-glucuronidase activity, detected two transgenic lines expressing the gene without apparent negative effects.Jóvenes Investigadores e Innovadores 812-2018MaestríaMagíster en Ciencias AgrariasGenética y fitomejoramientoxvii, 74 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias Agrarias - Maestría en Ciencias AgrariasEscuela de posgradosFacultad de Ciencias AgrariasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadasTransgenic plantsBiopolymersPlantas transgénicasBiopolímerosBiopolímeroTransgénicoβ-glucuronidasaPeroxisomaPéptido señalBiopolymerTransgenicβ-glucuronidasePeroxisomeSignal peptideLínea transgénica de Nicotiana tabacum expresando el gen phaC de Aeromonas caviae para la producción de polihidroxialcanoatosTransgenic line of Nicotiana tabacum expressing the phaC gene of Aeromonas caviae for the production of polyhydroxyalkanoatesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlmasi, M. 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