Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.

Ilustraciones

Autores:
Ocampo Gutierrez, Sebastian
Tipo de recurso:
Fecha de publicación:
2021
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
eng
OAI Identifier:
oai:repositorio.unal.edu.co:unal/80587
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/80587
https://repositorio.unal.edu.co/
Palabra clave:
620 - Ingeniería y operaciones afines
Ceramic materials
Materiales cerámicos
Hidrogel
Microscopía de fuerza de tracción
Microscopía holográfica
Partículas cerámicas
Suspensiones
Alginato
Hydrogel
Traction force microscopy
Holographic microscopy
Ceramic particles
Suspensions
Rights
openAccess
License
Atribución-NoComercial-CompartirIgual 4.0 Internacional
id UNACIONAL2_082ec901934a74885f1f87782e360391
oai_identifier_str oai:repositorio.unal.edu.co:unal/80587
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
dc.title.translated.eng.fl_str_mv Computational characterization of traction forces exerted over an alginate substrate optimized for additive manufacturing of scaffolds
title Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
spellingShingle Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
620 - Ingeniería y operaciones afines
Ceramic materials
Materiales cerámicos
Hidrogel
Microscopía de fuerza de tracción
Microscopía holográfica
Partículas cerámicas
Suspensiones
Alginato
Hydrogel
Traction force microscopy
Holographic microscopy
Ceramic particles
Suspensions
title_short Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
title_full Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
title_fullStr Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
title_full_unstemmed Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
title_sort Caracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.
dc.creator.fl_str_mv Ocampo Gutierrez, Sebastian
dc.contributor.advisor.none.fl_str_mv Rincón Fulla, Marlon
García García, Claudia Patricia
dc.contributor.author.none.fl_str_mv Ocampo Gutierrez, Sebastian
dc.contributor.researchgroup.spa.fl_str_mv Materiales Cerámicos y Vítreos
dc.subject.ddc.spa.fl_str_mv 620 - Ingeniería y operaciones afines
topic 620 - Ingeniería y operaciones afines
Ceramic materials
Materiales cerámicos
Hidrogel
Microscopía de fuerza de tracción
Microscopía holográfica
Partículas cerámicas
Suspensiones
Alginato
Hydrogel
Traction force microscopy
Holographic microscopy
Ceramic particles
Suspensions
dc.subject.lemb.none.fl_str_mv Ceramic materials
Materiales cerámicos
dc.subject.proposal.spa.fl_str_mv Hidrogel
Microscopía de fuerza de tracción
Microscopía holográfica
Partículas cerámicas
Suspensiones
Alginato
dc.subject.proposal.eng.fl_str_mv Hydrogel
Traction force microscopy
Holographic microscopy
Ceramic particles
Suspensions
description Ilustraciones
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-10-20T18:38:26Z
dc.date.available.none.fl_str_mv 2021-10-20T18:38:26Z
dc.date.issued.none.fl_str_mv 2021
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/80587
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/80587
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv eng
language eng
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dc.publisher.faculty.spa.fl_str_mv Facultad de Minas
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spelling Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rincón Fulla, Marlonf3890bf24c426e8f7626c1206ecb8395García García, Claudia Patricia6448197d2cc32832cb2f81989b4a9a99Ocampo Gutierrez, Sebastiana71689ac0b4923357840b28fc239fbc4Materiales Cerámicos y Vítreos2021-10-20T18:38:26Z2021-10-20T18:38:26Z2021https://repositorio.unal.edu.co/handle/unal/80587Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/IlustracionesCaracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds Muchos de los procesos o fenómenos que se estudian con células provienen de estudios donde se realizan cultivos en pozos con superficies rígidas y en monocapa. Si bien se puede extraer información importante de estos estudios, este ambiente de cultivo no es semejante al que encuentran las células in vivo. Por eso, es necesario desarrollar nuevos ambientes mas significativos fisiológicamente. En el presente trabajo se presenta una metodología para desarrollar un material de hidrogel de alginato con partículas cerámicas suspendidas en su volumen, con la potencialidad de soportar cultivos en 3D, y formar scaffolds con estructuras definidas matemáticamente a partir de superficies minimales triplemente periódicas. Con este material, que presenta propiedades elásticas y que muestra poder ser procesado por manufactura aditiva para obtener su forma final, se implementa la técnica de microscopía de fuerza de tracción, una técnica que permite ver las fuerzas ejercidas sobre el material aprovechando su elasticidad. Esta técnica, que es de caracter computacional, se implementa a través de la microscopía holográfica, con reconstrucciones de intensidad. De esta manera, se desarrolla un material que puede ser impreso en 3D, obtener scaffolds con estructuras complejas, y además recuperar mapas de distribución de fuerza ejercida sobre este a través de la microscopía de fuerza de tracción, usando la holografía digital sin lentes como método de captura de la información. (texto tomado de la fuente)Computational characterization of traction forces exerted over an alginate substrate optimized for additive manufacturing of scaffolds The general knowledge of many cell-based processes comes from experimentation on flat surfaces, mainly in culture wells made of either a polymer or glass. These are 2D, stiff and non-physiological environments, which are simple for experimentation, yet do not replicate all the conditions a cell experiences in vivo. In this work a methodology to develop a alginate hydrogel based material with ceramic particles suspension is presented. This material has the potential to act as a scaffold with a complex architecture processed via additive manufacturing, and is shown in the shape of a triply periodic minimal surface based structure. The formed hydrogel shows elastic properties suitable to implement traction force microscopy TFM, a computational technique to compute force fields exerted on the substrate. This is achieved using digital lensless holographic microscopy, a holographic technique that vastly reduces costs. The ceramic particles in the hydrogel are used as fiducial markers to calculate displacement in TFM. A material is developed based on alginate hydrogel, capable of complex architecture via additive manufacturing, suitable for 3D cell culturing. This material can also be used to measure forces exerted on it via traction force microscopy with help from holographic microscopy, using the embedded ceramic particles as fiducial markers. This way, a complex, more physiological environment can be achieved, and the means to recover some information are already proven to work on the material.Maestríax, 59 páginasapplication/pdfengUniversidad Nacional de ColombiaMedellín - Minas - Maestría en Ingeniería - Materiales y ProcesosDepartamento de Materiales y MineralesFacultad de MinasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín620 - Ingeniería y operaciones afinesCeramic materialsMateriales cerámicosHidrogelMicroscopía de fuerza de tracciónMicroscopía holográficaPartículas cerámicasSuspensionesAlginatoHydrogelTraction force microscopyHolographic microscopyCeramic particlesSuspensionsCaracterización computacional de la fuerza ejercida sobre un sustrato de hidrogel optimizado para manufactura aditiva de scaffolds.Computational characterization of traction forces exerted over an alginate substrate optimized for additive manufacturing of scaffoldsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMKarin A. 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GVyZWNob3MgZGUgYXV0b3IgcXVlIGNvbmxsZXZlIGxhIGRpc3RyaWJ1Y2nDs24gZGUgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuCkFsIGhhY2VyIGNsaWMgZW4gZWwgc2lndWllbnRlIGJvdMOzbiwgdXN0ZWQgaW5kaWNhIHF1ZSBlc3TDoSBkZSBhY3VlcmRvIGNvbiBlc3RvcyB0w6lybWlub3MuCgpVTklWRVJTSURBRCBOQUNJT05BTCBERSBDT0xPTUJJQSAtIMOabHRpbWEgbW9kaWZpY2FjacOzbiAyNy8yMC8yMDIwCg==

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