Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing.
ilustraciones, tablas
- Autores:
-
Pereira Bazurdo, Angie Natalia
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/80505
- Palabra clave:
- 580 - Plantas
660 - Ingeniería química
Aguacate - Enfermedades y plagas
Bacteria, phytopathogenic
Bacterias fitopatógenas
Patología vegetal
Actividad antifúngica,
Actividad antibacteriana
AHLs
Diketopiperazinas
Antifungal activity
Antibacterial activity
Diketopiperazines
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. |
| dc.title.translated.fra.fl_str_mv |
Characterization of bacterial strains obtained from thrips (Thysanoptera: Thripidae) that affect avocado crops (Persea americana Mill) in Antioquia: Antagonist activity and Quorum Sensing. |
| title |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. |
| spellingShingle |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. 580 - Plantas 660 - Ingeniería química Aguacate - Enfermedades y plagas Bacteria, phytopathogenic Bacterias fitopatógenas Patología vegetal Actividad antifúngica, Actividad antibacteriana AHLs Diketopiperazinas Antifungal activity Antibacterial activity Diketopiperazines |
| title_short |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. |
| title_full |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. |
| title_fullStr |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. |
| title_full_unstemmed |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. |
| title_sort |
Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing. |
| dc.creator.fl_str_mv |
Pereira Bazurdo, Angie Natalia |
| dc.contributor.advisor.none.fl_str_mv |
Moreno Herrera, Claudia Ximena Arango Isaza, Rafael Eduardo |
| dc.contributor.author.none.fl_str_mv |
Pereira Bazurdo, Angie Natalia |
| dc.contributor.researchgroup.spa.fl_str_mv |
Microbiodiversidad y Bioprospección |
| dc.subject.ddc.spa.fl_str_mv |
580 - Plantas 660 - Ingeniería química |
| topic |
580 - Plantas 660 - Ingeniería química Aguacate - Enfermedades y plagas Bacteria, phytopathogenic Bacterias fitopatógenas Patología vegetal Actividad antifúngica, Actividad antibacteriana AHLs Diketopiperazinas Antifungal activity Antibacterial activity Diketopiperazines |
| dc.subject.lemb.none.fl_str_mv |
Aguacate - Enfermedades y plagas Bacteria, phytopathogenic Bacterias fitopatógenas Patología vegetal |
| dc.subject.proposal.spa.fl_str_mv |
Actividad antifúngica, Actividad antibacteriana AHLs Diketopiperazinas |
| dc.subject.proposal.eng.fl_str_mv |
Antifungal activity Antibacterial activity Diketopiperazines |
| description |
ilustraciones, tablas |
| publishDate |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2021-10-12T13:09:30Z |
| dc.date.available.none.fl_str_mv |
2021-10-12T13:09:30Z |
| dc.date.issued.none.fl_str_mv |
2021 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/80505 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/80505 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Medellín - Ciencias - Maestría en Ciencias - Biotecnología |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Moreno Herrera, Claudia Ximena9e9b1162df5ae71da22de0e7cd708bd6Arango Isaza, Rafael Eduardob7e61322453072ebf7408a0f587e39c1Pereira Bazurdo, Angie Natalia99052070e1a529c24b1a6388cc847dc3Microbiodiversidad y Bioprospección2021-10-12T13:09:30Z2021-10-12T13:09:30Z2021https://repositorio.unal.edu.co/handle/unal/80505Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, tablasEl control de plagas y enfermedades del aguacate depende en gran medida del uso de pesticidas. Nuevas alternativas de control, tales como el uso de sustancias bioactivas inhibidoras contra fitopatógenos producidas por microorganismos, brindan una opción viable para contrarrestar las pérdidas por plagas y enfermedades. La exploración de nuevas fuentes de microorganismos se ha ampliado a especies que no habían sido consideradas. El presente estudio tuvo como objetivo caracterizar la capacidad antagonista in vitro contra microorganismos fitopatógenos y la producción de moléculas de señalización ‘Quórum Sensing’ (QS) en cepas bacterianas aisladas de trips de aguacate. La actividad antagonista de diez aislados bacterianos fueron seleccionadas y evaluadas contra siete fitopatógenos (hongos, oomiceto y bacterias) por cultivo dual, difusión y ensayo en placa; las moléculas de señalización (QS) producidas por las cepas Gram negativas se detectaron a través de bioensayo con placas de cromatografía fina (TLC) y biosensores bacterianos específicos (psB401, psB1142 y pqsA-lux). Las pruebas de cultivo dual mostraron que cuatro cepas pertenecientes a los géneros Bacillus, Pantoea, y Serratia, antagonizaron la mayoría de los hongos y el oomiceto, limitando el normal crecimiento de las colonias y generando zonas de inhibición. Adicionalmente, a través de biosensores se detectaron moléculas AHL y AHQ como 3-Oxo-C12-HSL(OdDHL), 3-Oxo-C6-HSL (OHHL) y 2-heptil-4-quinolona (HHQ). Trazas de OdDHL y HHQ, y el hallazgo de dos ciclopéptidos Ciclo(L-Phe-L-Pro) y Ciclo(L-Pro-L-Tyr) por Cromatografía Líquida de Ultra Alto Rendimiento y Espectrometría de Masas (UHPLC/MS) en los extractos de las cepas Gram negativas, podrían explicar la actividad antifúngica detectada en las dos cepas de Pantoea sp., y Serratia sp., ya que estas moléculas se caracterizan por estar relacionadas con los sistemas de QS y poseen una variedad amplia de actividad biológica. Hasta donde sabemos, este sería uno de los primeros reportes sobre el potencial antagonista y la detección de moléculas de señalización QS en cepas aisladas de trips de aguacate. (Texto tomado de la fuente)The control of avocado pests and diseases depends largely on the use of various types of pesticides, most of which are either strictly monitored or not accepted internationally. New alternatives such as inhibitory bioactive substances against phytopathogens produced by microorganisms provide an excellent alternative for the biocontrol of pests and diseases, and the exploration of new sources of these microorganisms has been extended to organisms previously not considered. This study aims to characterize the antagonistic capacity in vitro against phytopathogenic microorganisms and the production of Quorum Sensing (QS) signaling molecules from bacterial strains isolated from avocado thrips. The antagonist activity of ten bacterial isolates of thrips was evaluated against seven phytopathogens (fungi, oomycetes, and bacteria) by dual culture, diffusion, and plate assay; signaling molecules (QS) produced by Gram negative strains were detected through bioassay with fine chromatography plates (TLC) and specific bacterial biosensors (psB401, psB1142 and pqsA-lux). Dual culture tests showed that four strains belonging to the genera Bacillus sp., Pantoea sp., and Serratia sp., antagonized most of the fungi and the oomycete, limiting the normal growth of the hyphae and generating zones of inhibition. Additionally, AHL and AHQ molecules such as 3-Oxo-C12-HSL (OdDHL), 3-Oxo-C6-HSL (OHHL), and 2-heptyl-4-quinolone (HHQ) were detected through biosensors. Traces of OdDHL and HHQ, and the finding of two cyclopeptides Cycle(L-Phe-L-Pro) and Cycle(L-Pro-L-Tyr) by Ultra-High-Performance Liquid Chromatography and Mass Spectrometry (UHPLC / MS) in the extracts of the Gram negative strains could explain the antifungal activity detected in the two strains of Pantoea sp., and Serratia sp., since these molecules are characterized by being related to QS systems and possess a variety of biological activities including antimicrobial. To our knowledge, this would be one of the first reports on the antagonistic potential and the detection of QS signaling molecules in isolated strains of avocado thrips.MaestríaMagíster en Ciencias - BiotecnologíaBioprospección96 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaEscuela de biocienciasFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín580 - Plantas660 - Ingeniería químicaAguacate - Enfermedades y plagasBacteria, phytopathogenicBacterias fitopatógenasPatología vegetalActividad antifúngica,Actividad antibacterianaAHLsDiketopiperazinasAntifungal activityAntibacterial activityDiketopiperazinesCaracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing.Characterization of bacterial strains obtained from thrips (Thysanoptera: Thripidae) that affect avocado crops (Persea americana Mill) in Antioquia: Antagonist activity and Quorum Sensing.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAntioquia, ColombiaAlcaraz, M. 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Microbiology and Molecular Biology Reviews, 68(4), 745 LP – 770. https://doi.org/10.1128/MMBR.68.4.745-770.2004Universidad Nacional de ColombiaColcienciasInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/80505/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINAL1019088590.2021.pdf1019088590.2021.pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf2337778https://repositorio.unal.edu.co/bitstream/unal/80505/4/1019088590.2021.pdf2dcf8b14ebc53c52f533ea1d4e11d8d1MD54THUMBNAIL1019088590.2021.pdf.jpg1019088590.2021.pdf.jpgGenerated Thumbnailimage/jpeg5708https://repositorio.unal.edu.co/bitstream/unal/80505/5/1019088590.2021.pdf.jpg64de077908806f0a76f46020eb7da628MD55unal/80505oai:repositorio.unal.edu.co:unal/805052023-07-29 23:03:41.499Repositorio Institucional Universidad Nacional de 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