Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi
The treatement effect of a biotic elicitor fraction from the pathogen Fusarium oxysporum f. sp. dianthi (Fod), in the transcription of some defense genes on carnation roots (Dianthus caryophyllus L.) was evaluated. In a first stage, reference genes were selected for transcriptional studies in this p...
- Autores:
-
Monroy Mena, Santiago
- Tipo de recurso:
- Work document
- Fecha de publicación:
- 2020
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/77930
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/77930
- Palabra clave:
- 540 - Química y ciencias afines
570 - Biología
580 - Plantas
histona
ARNr18s
ARNseq
aminociclopropilcarboxilato oxidasa
factor de respuesta al etileno
dianthus caryophyllus
histone
RNAr18s
RNAseq
aminocyclopropylcarboxylate oxidase
ethylene response factor
dianthus caryophyllus
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- openAccess
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- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi |
| title |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi |
| spellingShingle |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi 540 - Química y ciencias afines 570 - Biología 580 - Plantas histona ARNr18s ARNseq aminociclopropilcarboxilato oxidasa factor de respuesta al etileno dianthus caryophyllus histone RNAr18s RNAseq aminocyclopropylcarboxylate oxidase ethylene response factor dianthus caryophyllus |
| title_short |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi |
| title_full |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi |
| title_fullStr |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi |
| title_full_unstemmed |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi |
| title_sort |
Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi |
| dc.creator.fl_str_mv |
Monroy Mena, Santiago |
| dc.contributor.advisor.spa.fl_str_mv |
Ardila Barrantes, Harold Duban Pinzón Velasco, Andres Mauricio |
| dc.contributor.author.spa.fl_str_mv |
Monroy Mena, Santiago |
| dc.contributor.corporatename.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.contributor.researchgroup.spa.fl_str_mv |
Estudio de actividades metabolicas vegetales |
| dc.subject.ddc.spa.fl_str_mv |
540 - Química y ciencias afines 570 - Biología 580 - Plantas |
| topic |
540 - Química y ciencias afines 570 - Biología 580 - Plantas histona ARNr18s ARNseq aminociclopropilcarboxilato oxidasa factor de respuesta al etileno dianthus caryophyllus histone RNAr18s RNAseq aminocyclopropylcarboxylate oxidase ethylene response factor dianthus caryophyllus |
| dc.subject.proposal.spa.fl_str_mv |
histona ARNr18s ARNseq aminociclopropilcarboxilato oxidasa factor de respuesta al etileno dianthus caryophyllus |
| dc.subject.proposal.eng.fl_str_mv |
histone RNAr18s RNAseq aminocyclopropylcarboxylate oxidase ethylene response factor dianthus caryophyllus |
| description |
The treatement effect of a biotic elicitor fraction from the pathogen Fusarium oxysporum f. sp. dianthi (Fod), in the transcription of some defense genes on carnation roots (Dianthus caryophyllus L.) was evaluated. In a first stage, reference genes were selected for transcriptional studies in this plant-pathogen interaction, finding that genes coding for an H3 histone and for the 18s ribosomal subunit can be used for this purpose. Subsequently, an in vivo assay was carried out to verify that the application of this elicitor fraction reduce the incidence of Fod disease in the carnation-susceptible cultivar. In the transcriptomic analysis, it was found that the effect of elicitation at the root level, caused overexpression at constitutive level of 1551 genes, of which 347 were related to functions in response to stress. In this category, it was determined that among others, there are genes that code for proteins related to pathogenesis (PRs) such as β-1-3 endoglucanases and chitinases, enzymes involved in biosynthetic pathways of secondary metabolites, proteins associated with the recognition of PAMPs and MAMPs (molecular paterns associated with pathogens and microorganisms recognition, respectively) and transcription factors in response to ethylene. Finally, the transcriptional levels for 4 of these genes were compared during the pathogen inoculation, in treatments previously treated with the elicitor fraction and control treatments without elicitation. It was determined that elicitation potentiated the expression of an aminocyclopropylcarboxylate oxidase enzyme related to the biosynthesis of ethylene and of a protein acting as a response factor to this hormone. These results suggest that elicitation potentiates the signaling pathways associated with this hormone which may be important in the induction of resistance in this pathosystem. |
| publishDate |
2020 |
| dc.date.accessioned.spa.fl_str_mv |
2020-08-05T07:45:53Z |
| dc.date.available.spa.fl_str_mv |
2020-08-05T07:45:53Z |
| dc.date.issued.spa.fl_str_mv |
2020-02-14 |
| dc.type.spa.fl_str_mv |
Documento de trabajo |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/workingPaper |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_8042 |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/WP |
| format |
http://purl.org/coar/resource_type/c_8042 |
| status_str |
acceptedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Monroy Mena, S. (2020). Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi. Departamento de Quimica, Facultad de Ciencias, Universidad Nacional de Colombia. |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/77930 |
| identifier_str_mv |
Monroy Mena, S. (2020). Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi. Departamento de Quimica, Facultad de Ciencias, Universidad Nacional de Colombia. |
| url |
https://repositorio.unal.edu.co/handle/unal/77930 |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial-SinDerivadas 4.0 InternacionalDerechos reservados - Universidad Nacional de ColombiaAcceso abiertohttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ardila Barrantes, Harold Duban31e17f3f-d386-4972-90f5-07d2addfe9d9-1Pinzón Velasco, Andres Mauricio51de7445-1b24-49fd-b3be-d3631cd6bf03-1Monroy Mena, Santiago8a19e55b-538e-462f-8bcb-22b2db27298aUniversidad Nacional de ColombiaEstudio de actividades metabolicas vegetales2020-08-05T07:45:53Z2020-08-05T07:45:53Z2020-02-14Monroy Mena, S. (2020). Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi. Departamento de Quimica, Facultad de Ciencias, Universidad Nacional de Colombia.https://repositorio.unal.edu.co/handle/unal/77930The treatement effect of a biotic elicitor fraction from the pathogen Fusarium oxysporum f. sp. dianthi (Fod), in the transcription of some defense genes on carnation roots (Dianthus caryophyllus L.) was evaluated. In a first stage, reference genes were selected for transcriptional studies in this plant-pathogen interaction, finding that genes coding for an H3 histone and for the 18s ribosomal subunit can be used for this purpose. Subsequently, an in vivo assay was carried out to verify that the application of this elicitor fraction reduce the incidence of Fod disease in the carnation-susceptible cultivar. In the transcriptomic analysis, it was found that the effect of elicitation at the root level, caused overexpression at constitutive level of 1551 genes, of which 347 were related to functions in response to stress. In this category, it was determined that among others, there are genes that code for proteins related to pathogenesis (PRs) such as β-1-3 endoglucanases and chitinases, enzymes involved in biosynthetic pathways of secondary metabolites, proteins associated with the recognition of PAMPs and MAMPs (molecular paterns associated with pathogens and microorganisms recognition, respectively) and transcription factors in response to ethylene. Finally, the transcriptional levels for 4 of these genes were compared during the pathogen inoculation, in treatments previously treated with the elicitor fraction and control treatments without elicitation. It was determined that elicitation potentiated the expression of an aminocyclopropylcarboxylate oxidase enzyme related to the biosynthesis of ethylene and of a protein acting as a response factor to this hormone. These results suggest that elicitation potentiates the signaling pathways associated with this hormone which may be important in the induction of resistance in this pathosystem.Se evaluó el efecto que tiene la aplicación de una fracción elicitora de origen biótico proveniente del patógeno Fusarium oxysporum f. sp. dianthi (Fod), en la transcripción de algunos genes de defensa en raíces del clavel (Dianthus caryophyllus L.). Para ello, en una primera etapa se encontró que los genes codificantes para una histona H3 y para la subunidad ribosomal 18s, pueden ser usados como genes de referencia para estudios transcripcionales en esta interacción planta patógeno. Posteriormente en un ensayo in vivo se verificó que la aplicación del elicitor, tiene un efecto en la disminución de la incidencia a la enfermedad causada por Fod en la variedad susceptible de clavel. Se determinó en el análisis transcriptómico preliminar que la elicitación, generó un aumento en los niveles de transcripción de 1551 genes de los cuales, 347 se encontraban relacionados a funciones como respuesta a estrés. En esta categoría, se encuentran genes que codifican para proteínas relacionadas con patogénesis como β-1-3 endoglucanasas y quitinasas, enzimas involucradas en rutas biosintéticas de metabolitos secundarios, proteínas asociadas con el reconocimiento de PAMPs y MAMPs (patrones moleculares asociados a patógenos y microorganismos, respectivamente) y factores de transcripción de respuesta al etileno. Finalmente se compararon los niveles transcripcionales durante la inoculación con el patógeno, de 4 de los genes con potencial expresión diferencial, en tratamientos con o sin elicitación. Se determinó que la elicitación potencializó la expresión de una enzima aminociclopropilcarboxilato oxidasa relacionada con la biosíntesis del etileno y de una proteína que actúa como factor de respuesta a esta hormona. Estos resultados sugieren que la elicitación potencializa las rutas de señalización asociadas con esta hormona la cual puede ser central en la inducción de resistencia en este patosistema.ColcienciasEstudio del uso de elicitores de origen biótico en el clavel (Dianthus caryophyllus L) para el control del marchitamiento vascular: Una alternativa al uso de fungicidas de origen sintético” (Código 110174558226)Línea de Investigación: Bioquímica de las Interacciones Hospedero-Patógeno.Maestría130application/pdfspa540 - Química y ciencias afines570 - Biología580 - PlantashistonaARNr18sARNseqaminociclopropilcarboxilato oxidasafactor de respuesta al etilenodianthus caryophyllushistoneRNAr18sRNAseqaminocyclopropylcarboxylate oxidaseethylene response factordianthus caryophyllusEfecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthiDocumento de trabajoinfo:eu-repo/semantics/workingPaperinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_8042Texthttp://purl.org/redcol/resource_type/WPBogotá - Ciencias - Maestría en Ciencias - BioquímicaDepartamento de QuímicaUniversidad Nacional de Colombia - Sede BogotáAdie, B., Chico, J. 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