Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi

The treatement effect of a biotic elicitor fraction from the pathogen Fusarium oxysporum f. sp. dianthi (Fod), in the transcription of some defense genes on carnation roots (Dianthus caryophyllus L.) was evaluated. In a first stage, reference genes were selected for transcriptional studies in this p...

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Autores:: Monroy Mena, Santiago

Tipo de recurso:: Work document

Fecha de publicación:: 2020

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

Description
Summary:	The treatement effect of a biotic elicitor fraction from the pathogen Fusarium oxysporum f. sp. dianthi (Fod), in the transcription of some defense genes on carnation roots (Dianthus caryophyllus L.) was evaluated. In a first stage, reference genes were selected for transcriptional studies in this plant-pathogen interaction, finding that genes coding for an H3 histone and for the 18s ribosomal subunit can be used for this purpose. Subsequently, an in vivo assay was carried out to verify that the application of this elicitor fraction reduce the incidence of Fod disease in the carnation-susceptible cultivar. In the transcriptomic analysis, it was found that the effect of elicitation at the root level, caused overexpression at constitutive level of 1551 genes, of which 347 were related to functions in response to stress. In this category, it was determined that among others, there are genes that code for proteins related to pathogenesis (PRs) such as β-1-3 endoglucanases and chitinases, enzymes involved in biosynthetic pathways of secondary metabolites, proteins associated with the recognition of PAMPs and MAMPs (molecular paterns associated with pathogens and microorganisms recognition, respectively) and transcription factors in response to ethylene. Finally, the transcriptional levels for 4 of these genes were compared during the pathogen inoculation, in treatments previously treated with the elicitor fraction and control treatments without elicitation. It was determined that elicitation potentiated the expression of an aminocyclopropylcarboxylate oxidase enzyme related to the biosynthesis of ethylene and of a protein acting as a response factor to this hormone. These results suggest that elicitation potentiates the signaling pathways associated with this hormone which may be important in the induction of resistance in this pathosystem.

Efecto de elicitores de origen biótico en la transcripción de algunos genes involucrados en los mecanismos de defensa del clavel Dianthus caryophyllus L. al patógeno Fusarium oxysporum f sp dianthi

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