Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas
La cicatrización de úlceras crónicas de pie diabético (UCPD), es un proceso complejo y dinámico que requiere de una interacción entre factores regulados que trabajan en conjunto para restituir la piel lesionada. Los niveles altos de metaloproteinasas de matriz (MPMs) en las UCPD contribuyen a la cro...
- Autores:
-
Bayona Velasco, Geydi Alexandra
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2020
- Institución:
- Universidad Autónoma de Bucaramanga - UNAB
- Repositorio:
- Repositorio UNAB
- Idioma:
- spa
- OAI Identifier:
- oai:repository.unab.edu.co:20.500.12749/11845
- Acceso en línea:
- http://hdl.handle.net/20.500.12749/11845
- Palabra clave:
- Biomedical engineering
Engineering
Medical electronics
Biological physics
Bioengineering
Medical instruments and apparatus
Medicine
Diacrylated polyethylene
Glycol
Hydrogels
Mesh size
Modulus of elasticity
Extracellular matrix
Granulation tissue
Foot diseases
Polymethylmethacrylate
Ingeniería biomédica
Ingeniería
Biofísica
Bioingeniería
Medicina
Tejido de granulación
Enfermedades de los pies
Polimetilmetacrilato
Ingeniería clínica
Clinical engineering
Electrónica médica
Instrumentos y aparatos médicos
Polietilenglicol diacrilado
Hidrogeles
Tamaño de poro
Módulo de elasticidad
Matriz extracelular
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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| dc.title.spa.fl_str_mv |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas |
| dc.title.translated.spa.fl_str_mv |
Evaluation of the mechanical properties of biodegradable hydrogels based on diacrylated polyethylene glycol, with potential use in the design of matrices for chronic ulcers |
| title |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas |
| spellingShingle |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine Diacrylated polyethylene Glycol Hydrogels Mesh size Modulus of elasticity Extracellular matrix Granulation tissue Foot diseases Polymethylmethacrylate Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Tejido de granulación Enfermedades de los pies Polimetilmetacrilato Ingeniería clínica Clinical engineering Electrónica médica Instrumentos y aparatos médicos Polietilenglicol diacrilado Hidrogeles Tamaño de poro Módulo de elasticidad Matriz extracelular |
| title_short |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas |
| title_full |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas |
| title_fullStr |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas |
| title_full_unstemmed |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas |
| title_sort |
Evaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicas |
| dc.creator.fl_str_mv |
Bayona Velasco, Geydi Alexandra |
| dc.contributor.advisor.spa.fl_str_mv |
Solarte David, Víctor Alfonso Becerra Bayona, Silvia Milena |
| dc.contributor.author.spa.fl_str_mv |
Bayona Velasco, Geydi Alexandra |
| dc.contributor.cvlac.*.fl_str_mv |
https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001363781 |
| dc.contributor.cvlac.none.fl_str_mv |
Becerra Bayona, Silvia Milena [0001568861] |
| dc.contributor.googlescholar.none.fl_str_mv |
Becerra Bayona, Silvia Milena [5wr21EQAAAAJ] |
| dc.contributor.orcid.none.fl_str_mv |
Becerra Bayona, Silvia Milena [0000-0002-4499-5885] |
| dc.contributor.scopus.none.fl_str_mv |
Becerra Bayona, Silvia Milena [36522328100] |
| dc.contributor.researchgate.none.fl_str_mv |
Becerra Bayona, Silvia Milena [Silvia-Becerra-Bayona] |
| dc.contributor.apolounab.none.fl_str_mv |
Becerra Bayona, Silvia Milena [silvia-milena-becerra-bayona] |
| dc.contributor.linkedin.none.fl_str_mv |
Becerra Bayona, Silvia Milena [silvia-becerra-3174455a] |
| dc.subject.keywords.eng.fl_str_mv |
Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine Diacrylated polyethylene Glycol Hydrogels Mesh size Modulus of elasticity Extracellular matrix Granulation tissue Foot diseases Polymethylmethacrylate |
| topic |
Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine Diacrylated polyethylene Glycol Hydrogels Mesh size Modulus of elasticity Extracellular matrix Granulation tissue Foot diseases Polymethylmethacrylate Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Tejido de granulación Enfermedades de los pies Polimetilmetacrilato Ingeniería clínica Clinical engineering Electrónica médica Instrumentos y aparatos médicos Polietilenglicol diacrilado Hidrogeles Tamaño de poro Módulo de elasticidad Matriz extracelular |
| dc.subject.lemb.spa.fl_str_mv |
Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Tejido de granulación Enfermedades de los pies Polimetilmetacrilato |
| dc.subject.proposal.spa.fl_str_mv |
Ingeniería clínica Clinical engineering Electrónica médica Instrumentos y aparatos médicos Polietilenglicol diacrilado Hidrogeles Tamaño de poro Módulo de elasticidad Matriz extracelular |
| description |
La cicatrización de úlceras crónicas de pie diabético (UCPD), es un proceso complejo y dinámico que requiere de una interacción entre factores regulados que trabajan en conjunto para restituir la piel lesionada. Los niveles altos de metaloproteinasas de matriz (MPMs) en las UCPD contribuyen a la cronicidad de la herida, lo que puede llevar a incrementar el riesgo de infecciones, provocando complicaciones que influyen en la calidad de vida del paciente. Por lo tanto, existe la necesidad de desarrollar alternativas terapéuticas que permitan la correcta cicatrización de la herida; entre los nuevos enfoques se destacan el uso de hidrogeles de polietilenglicol diacrilado (PEGDA), el cual se puede modificar mediante la introducción de dominios de corte para MPMs con el fin de que, las altas concentraciones de estas proteasas en la úlcera sean aprovechadas para degradar el material sintético. En consecuencia, en este estudio se fabricaron hidrogeles biodegradables derivados de PEGDA (7.34 kDa), en tres diferentes concentraciones (10, 20 y 30% p/v); los hidrogeles se polimerizaron en luz UV, en presencia de dos sistemas diferentes de fotoiniciador (Irgacure 2959, disuelto en NVP o en 70% etanol), las propiedades estructurales del hidrogel como la capacidad de hinchamiento, se evaluó a partir de la relación volumétrica de hinchamiento y el tamaño de poro teórico se calculó mediante la correlación con el módulo elástico experimental, mientras que el módulo elástico y de compresión fueron determinados mediante pruebas de tracción y compresión. Los resultados obtenidos de los hidrogeles de PEGDA biodegradables por MPMs, demuestran que, poseen excelentes propiedades mecánicas y físicas, ya que son similares a la piel; por lo tanto, podrían tener gran potencial en la curación de UCPD. |
| publishDate |
2020 |
| dc.date.accessioned.none.fl_str_mv |
2020-12-03T17:11:50Z |
| dc.date.available.none.fl_str_mv |
2020-12-03T17:11:50Z |
| dc.date.issued.none.fl_str_mv |
2020 |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
| dc.type.local.spa.fl_str_mv |
Trabajo de Grado |
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http://purl.org/coar/resource_type/c_7a1f |
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http://purl.org/redcol/resource_type/TP |
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http://hdl.handle.net/20.500.12749/11845 |
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instname:Universidad Autónoma de Bucaramanga - UNAB |
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reponame:Repositorio Institucional UNAB |
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repourl:https://repository.unab.edu.co |
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http://hdl.handle.net/20.500.12749/11845 |
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instname:Universidad Autónoma de Bucaramanga - UNAB reponame:Repositorio Institucional UNAB repourl:https://repository.unab.edu.co |
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spa |
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spa |
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Controlled proteolytic cleavage site presentation in biomimetic PEGDA hydrogels enhances neovascularization in vitro. Tissue Engineering - Part A, 18(23–24), 2477–2486. https://doi.org/10.1089/ten.tea.2012.0173 Tessmar, J. K., & Göpferich, A. M. (2007). Customized PEG-derived copolymers for tissue engineering applications. Macromolecular Bioscience, 7(1), 23–39. https://doi.org/10.1002/mabi.200600096 Vargas-Uricoechea, H., & Casas-Figueroa, L. Á. (2016). Epidemiology of diabetes mellitus in South America: The experience of Colombia. Clinica e Investigacion En Arteriosclerosis, 28(5), 245–256. https://doi.org/10.1016/j.arteri.2015.12.002 Zahouani, H., Pailler-Mattei, C., Sohm, B., Vargiolu, R., Cenizo, V., & Debret, R. (2009). Characterization of the mechanical properties of a dermal equivalent compared with human skin in vivo by indentation and static friction tests. Skin Research and Technology, 15(1), 68–76. https://doi.org/10.1111/j.1600-0846.2008.00329.x |
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Repositorio Institucional | Universidad Autónoma de Bucaramanga - UNAB |
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Solarte David, Víctor Alfonso54590e96-eda3-4b43-9ffa-14bd35ed7d08Becerra Bayona, Silvia Milenaf59fde3b-924f-4fcc-96e9-5fd6250b2daeBayona Velasco, Geydi Alexandrafed80c46-d18e-473a-b7ef-9ad7bf3688e7https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001363781Becerra Bayona, Silvia Milena [0001568861]Becerra Bayona, Silvia Milena [5wr21EQAAAAJ]Becerra Bayona, Silvia Milena [0000-0002-4499-5885]Becerra Bayona, Silvia Milena [36522328100]Becerra Bayona, Silvia Milena [Silvia-Becerra-Bayona]Becerra Bayona, Silvia Milena [silvia-milena-becerra-bayona]Becerra Bayona, Silvia Milena [silvia-becerra-3174455a]ColombiaUNAB Campus Bucaramanga2020-12-03T17:11:50Z2020-12-03T17:11:50Z2020http://hdl.handle.net/20.500.12749/11845instname:Universidad Autónoma de Bucaramanga - UNABreponame:Repositorio Institucional UNABrepourl:https://repository.unab.edu.coLa cicatrización de úlceras crónicas de pie diabético (UCPD), es un proceso complejo y dinámico que requiere de una interacción entre factores regulados que trabajan en conjunto para restituir la piel lesionada. Los niveles altos de metaloproteinasas de matriz (MPMs) en las UCPD contribuyen a la cronicidad de la herida, lo que puede llevar a incrementar el riesgo de infecciones, provocando complicaciones que influyen en la calidad de vida del paciente. Por lo tanto, existe la necesidad de desarrollar alternativas terapéuticas que permitan la correcta cicatrización de la herida; entre los nuevos enfoques se destacan el uso de hidrogeles de polietilenglicol diacrilado (PEGDA), el cual se puede modificar mediante la introducción de dominios de corte para MPMs con el fin de que, las altas concentraciones de estas proteasas en la úlcera sean aprovechadas para degradar el material sintético. En consecuencia, en este estudio se fabricaron hidrogeles biodegradables derivados de PEGDA (7.34 kDa), en tres diferentes concentraciones (10, 20 y 30% p/v); los hidrogeles se polimerizaron en luz UV, en presencia de dos sistemas diferentes de fotoiniciador (Irgacure 2959, disuelto en NVP o en 70% etanol), las propiedades estructurales del hidrogel como la capacidad de hinchamiento, se evaluó a partir de la relación volumétrica de hinchamiento y el tamaño de poro teórico se calculó mediante la correlación con el módulo elástico experimental, mientras que el módulo elástico y de compresión fueron determinados mediante pruebas de tracción y compresión. Los resultados obtenidos de los hidrogeles de PEGDA biodegradables por MPMs, demuestran que, poseen excelentes propiedades mecánicas y físicas, ya que son similares a la piel; por lo tanto, podrían tener gran potencial en la curación de UCPD.Capítulo 1 Problema u oportunidad ...........................................................................................10 1.1 Introducción ............................................................................................................10 1.2 Planteamiento del problema.....................................................................................11 1.3 Justificación ............................................................................................................12 1.4 Pregunta Problema ..................................................................................................13 1.5 Objetivo General .....................................................................................................13 1.6 Objetivos específicos...............................................................................................13 1.7 Limitaciones y delimitaciones .................................................................................14 Capítulo 2 Marco teórico..........................................................................................................15 2.1 La piel .....................................................................................................................15 2.2 Úlcera de pie diabético ............................................................................................21 2.3 Andamios................................................................................................................24 2.4 Hidrogeles...............................................................................................................25 2.4.1. Estructura de los hidrogeles .................................................................................25 2.4.2. Capacidad de hinchamiento de los hidrogeles.......................................................26 2.4.3. Correlación entre la capacidad de hinchamiento y el tamaño de poro de los hidrogeles..........................................................................................................................27 2.5 Hidrogeles de polietilenglicol diacrilado (PEGDA) .................................................29 2.5.1. Propiedades mecánicas del PEGDA .....................................................................30 2.5.2. Degradación del hidrogel de PEGDA...................................................................31 Capítulo 3 Estado del arte..........................................................................................................33 Capítulo 4 Metodología.............................................................................................................37 4.1 Síntesis del copolímero derivado de PEGDA con secuencias peptídicas degradables (PEGDA-BD)....................................................................................................................37 4.2 Fabricación de los hidrogeles biodegradables derivados de PEGDA. .......................37 4.3 Evaluación de la capacidad de hinchamiento de los hidrogeles ................................38 4.4 Evaluación del tamaño de poro de los hidrogeles.....................................................38 4.5 Caracterización mecánica de los hidrogeles.............................................................39 4.6 Análisis estadísticos.................................................................................................40 Capítulo 5 Resultados y Análisis de Resultados.........................................................................41 5.1 Resultados...................................................................................................................41 5.1.1. Síntesis y fabricación de los hidrogeles de PEGDA-BD .......................................41 5.1.2. Caracterización mecánica de los hidrogeles de PEGDA-BD y PEGDA-Control...42 5.1.3. Evaluación de la capacidad de hinchamiento de los hidrogeles.............................47 5.1.4. Evaluación del tamaño de poro promedio de los hidrogeles..................................51 5.1.5. Relación entre el Módulo elástico y la capacidad de hinchamiento de los hidrogeles en función de la densidad de entrecruzamiento. .................................................................55 5.2 Análisis de resultados..............................................................................................57 Capítulo 6 Conclusiones y recomendaciones.............................................................................62 Lista de referencias ...................................................................................................................64 Apéndice...................................................................................................................................68PregradoHealing of chronic diabetic foot ulcers (CDFU) is a complex and dynamic process that requires an interaction between regulated factors that work together to restore damaged skin. The high levels of matrix metalloproteinases (MMP) in the CDFU affect the chronicity of the wound, which can lead to an increased risk of infections, causing complications that influence the quality of life of the patient. Therefore, there is a need to develop therapeutic alternatives that could correct wound healing; New approaches include the use of diacrylated polyethylene glycol (PEGDA) hydrogels, which can be modified by introducing cut domains for MMPs so that high concentrations of these proteases in the ulcer are exploited to degrade Synthetic material. Consequently, in this study, biodegradable hydrogels derived from PEGDA (7.34 kDa) were manufactured, in three different concentrations (10, 20 and 30% w / v); hydrogels were polymerized in UV light, in the presence of two different photoinitiator systems (Irgacure 2959, dissolved in NVP or in 70% ethanol), the structural properties of the hydrogel, as well as the swelling capacity, were evaluated from the volumetric ratio of swelling and theoretical pore size will be calculated by correlation with the experimental elastic modulus, while the elastic and compression modulus were determined by tensile and compression tests. The results obtained from PEGDA hydrogels biodegradable by MPM, could have excellent mechanical and physical properties, since they are similar to the skin; therefore, it could have great potential in the healing of CDFU.Modalidad Presencialapplication/pdfspahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 2.5 ColombiaEvaluación de las propiedades mecánicas de hidrogeles biodegradables a base de polietilenglicol diacrilado, con potencial uso en el diseño de matrices para úlceras crónicasEvaluation of the mechanical properties of biodegradable hydrogels based on diacrylated polyethylene glycol, with potential use in the design of matrices for chronic ulcersIngeniero BiomédicoUniversidad Autónoma de Bucaramanga UNABFacultad IngenieríaPregrado Ingeniería Biomédicainfo:eu-repo/semantics/bachelorThesisTrabajo de Gradohttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/redcol/resource_type/TPBiomedical engineeringEngineeringMedical electronicsBiological physicsBioengineeringMedical instruments and apparatusMedicineDiacrylated polyethyleneGlycolHydrogelsMesh sizeModulus of elasticityExtracellular matrixGranulation tissueFoot diseasesPolymethylmethacrylateIngeniería biomédicaIngenieríaBiofísicaBioingenieríaMedicinaTejido de granulaciónEnfermedades de los piesPolimetilmetacrilatoIngeniería clínicaClinical engineeringElectrónica médicaInstrumentos y aparatos médicosPolietilenglicol diacriladoHidrogelesTamaño de poroMódulo de elasticidadMatriz extracelularAguirre-Soto, A., Kim, S., Kaastrup, K., & Sikes, H. 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Skin Research and Technology, 15(1), 68–76. https://doi.org/10.1111/j.1600-0846.2008.00329.xORIGINAL2017_Tesis_Geydi_Alexandra_Bayona.pdf2017_Tesis_Geydi_Alexandra_Bayona.pdfTesisapplication/pdf900672https://repository.unab.edu.co/bitstream/20.500.12749/11845/1/2017_Tesis_Geydi_Alexandra_Bayona.pdfa7b2c76b701feaa7bc078f6a86c5d41aMD51open access2017_Licencia_Geydi_Alexandra_Bayona_1.pdf2017_Licencia_Geydi_Alexandra_Bayona_1.pdfLicenciaapplication/pdf509121https://repository.unab.edu.co/bitstream/20.500.12749/11845/2/2017_Licencia_Geydi_Alexandra_Bayona_1.pdf0418e1e2efe5e859dc612c5e45e907b0MD52metadata only accessTHUMBNAIL2017_Tesis_Geydi_Alexandra_Bayona.pdf.jpg2017_Tesis_Geydi_Alexandra_Bayona.pdf.jpgimage/jpeg5078https://repository.unab.edu.co/bitstream/20.500.12749/11845/3/2017_Tesis_Geydi_Alexandra_Bayona.pdf.jpg48be2aeeb6b9a664ca397d13b5c3afd3MD53open access2017_Licencia_Geydi_Alexandra_Bayona_1.pdf.jpg2017_Licencia_Geydi_Alexandra_Bayona_1.pdf.jpgIM Thumbnailimage/jpeg9702https://repository.unab.edu.co/bitstream/20.500.12749/11845/4/2017_Licencia_Geydi_Alexandra_Bayona_1.pdf.jpge50ca3ee389a095461d700d1ef50d415MD54metadata only access20.500.12749/11845oai:repository.unab.edu.co:20.500.12749/118452023-11-25 03:47:26.492open accessRepositorio Institucional | Universidad Autónoma de Bucaramanga - UNABrepositorio@unab.edu.co |