Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético
La diabetes mellitus es una enfermedad que en la actualidad se considera un problema de salud pública y se estima que afecte a más de 700 millones de personas adultas en los años venideros. Una de sus principales complicaciones son las úlceras crónicas de pie diabético (UCPD), lesiones cutáneas que...
- Autores:
-
Rojas Cárdenas, Luis David
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad Autónoma de Bucaramanga - UNAB
- Repositorio:
- Repositorio UNAB
- Idioma:
- spa
- OAI Identifier:
- oai:repository.unab.edu.co:20.500.12749/19050
- Acceso en línea:
- http://hdl.handle.net/20.500.12749/19050
- Palabra clave:
- Biomedical engineering
Engineering
Medical electronics
Biological physics
Bioengineering
Medical instruments and apparatus
Medicine
Biomedical
Clinical engineering
Collagen
Cytotoxicity
Fibroblasts
Hydrogels
Platelet poor plasma
Mellitus diabetes
Foot diseases
Feet (Ulcers)
Ingeniería biomédica
Ingeniería
Biofísica
Bioingeniería
Medicina
Biomédica
Diabetes mellitus
Enfermedades de lo pies
Pies (Ulceras)
Ingeniería clínica
Electrónica médica
Instrumentos y aparatos médicos
Colágeno
Citotoxicidad
Fibroblastos
Hidrogeles
Plasma pobre en plaquetas
- Rights
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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| dc.title.spa.fl_str_mv |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético |
| dc.title.translated.spa.fl_str_mv |
Development of a platelet-poor plasma hydrogel-type dressing and collagen extracted from tilapia skin with potential use for the treatment of chronic diabetic foot ulcers |
| title |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético |
| spellingShingle |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine Biomedical Clinical engineering Collagen Cytotoxicity Fibroblasts Hydrogels Platelet poor plasma Mellitus diabetes Foot diseases Feet (Ulcers) Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Biomédica Diabetes mellitus Enfermedades de lo pies Pies (Ulceras) Ingeniería clínica Electrónica médica Instrumentos y aparatos médicos Colágeno Citotoxicidad Fibroblastos Hidrogeles Plasma pobre en plaquetas |
| title_short |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético |
| title_full |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético |
| title_fullStr |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético |
| title_full_unstemmed |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético |
| title_sort |
Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabético |
| dc.creator.fl_str_mv |
Rojas Cárdenas, Luis David |
| dc.contributor.advisor.none.fl_str_mv |
Solarte David, Víctor Alfonso Becerra Bayona, Silvia Milena |
| dc.contributor.author.none.fl_str_mv |
Rojas Cárdenas, Luis David |
| dc.contributor.cvlac.spa.fl_str_mv |
Solarte David, Víctor Alfonso [0001329391] Becerra Bayona, Silvia Milena [0001568861] |
| dc.contributor.googlescholar.spa.fl_str_mv |
Becerra Bayona, Silvia Milena [5wr21EQAAAAJ] |
| dc.contributor.orcid.spa.fl_str_mv |
Solarte David, Víctor Alfonso [0000-0002-9856-1484] Becerra Bayona, Silvia Milena [0000-0002-4499-5885] |
| dc.contributor.scopus.spa.fl_str_mv |
Becerra Bayona, Silvia Milena [36522328100] |
| dc.contributor.researchgate.spa.fl_str_mv |
Solarte David, Víctor Alfonso [Victor-Solarte-David] Becerra Bayona, Silvia Milena [Silvia-Becerra-Bayona] |
| dc.contributor.apolounab.spa.fl_str_mv |
Solarte David, Víctor Alfonso [víctor-alfonso-solarte-david] Becerra Bayona, Silvia Milena [silvia-milena-becerra-bayona] |
| dc.contributor.linkedin.none.fl_str_mv |
Becerra Bayona, Silvia Milena [silvia-becerra-3174455a] |
| dc.subject.keywords.spa.fl_str_mv |
Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine Biomedical Clinical engineering Collagen Cytotoxicity Fibroblasts Hydrogels Platelet poor plasma Mellitus diabetes Foot diseases Feet (Ulcers) |
| topic |
Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine Biomedical Clinical engineering Collagen Cytotoxicity Fibroblasts Hydrogels Platelet poor plasma Mellitus diabetes Foot diseases Feet (Ulcers) Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Biomédica Diabetes mellitus Enfermedades de lo pies Pies (Ulceras) Ingeniería clínica Electrónica médica Instrumentos y aparatos médicos Colágeno Citotoxicidad Fibroblastos Hidrogeles Plasma pobre en plaquetas |
| dc.subject.lemb.spa.fl_str_mv |
Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Biomédica Diabetes mellitus Enfermedades de lo pies Pies (Ulceras) |
| dc.subject.proposal.spa.fl_str_mv |
Ingeniería clínica Electrónica médica Instrumentos y aparatos médicos Colágeno Citotoxicidad Fibroblastos Hidrogeles Plasma pobre en plaquetas |
| description |
La diabetes mellitus es una enfermedad que en la actualidad se considera un problema de salud pública y se estima que afecte a más de 700 millones de personas adultas en los años venideros. Una de sus principales complicaciones son las úlceras crónicas de pie diabético (UCPD), lesiones cutáneas que en la actualidad no tienen tratamientos 100% efectivos y que afectan de manera significativa la calidad de vida de quienes la padecen, lo que ha incrementado la necesidad de encontrar tratamientos que mejoren el proceso de cicatrización de este tipo de heridas. Por lo anterior, en el presente proyecto se ha sido utilizado piel de tilapia roja, un desecho de la industria acuícola, para extraer colágeno y con este elaborar hidrogeles, los cuales se reticularon con plasma pobre en plaquetas (PPP), una fracción de la sangre que no ha sido frecuentemente utilizada para la investigación. El proceso de extracción de colágeno ácido soluble permitió obtener un rendimiento en base seca cercano al 40%, y a partir de este, fabricar los hidrogeles y reticularlos con el PPP, obteniendo una dinámica adecuada en cuanto a la liberación de proteínas. Estos hidrogeles se sometieron a diferentes ensayos con el fin de determinar sus propiedades mecánicas y físicas, encontrando módulos de compresión similares a los de las capas internas de la piel, así como una capacidad de hinchamiento óptima, lo que les permitiría entregar la humedad necesaria a la herida durante el proceso de cicatrización. Finalmente, a partir de pruebas in vitro, se determinó que el lixiviado producido por los hidrogeles mantiene la viabilidad celular en un periodo de 48 horas, al permitir la proliferación de fibroblastos de la línea celular HT1080. Estos resultados indican que los hidrogeles fabricados en el presente estudio podrían ser una alternativa terapéutica para el tratamiento de las UCPD. |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2023-02-20T14:22:21Z |
| dc.date.available.none.fl_str_mv |
2023-02-20T14:22:21Z |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
| dc.type.local.spa.fl_str_mv |
Trabajo de Grado |
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http://purl.org/coar/resource_type/c_7a1f |
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Biomaterials Science: An Evolving, Multidisciplinary Endeavor. Biomaterials Science: An Introduction to Materials: Third Edition, xxv–xxxix. https://doi.org/10.1016/B978-0-08-087780-8.00153-4 Ramírez Rojas, D., Ramírez Sánchez, P., Santos Soto, J. (2022). Evaluación de las propiedades mecánicas de hidrogeles a base de colágeno de piel de tilapia con potencial uso en el tratamiento de quemaduras de segundo grado [Tesis de pregrado]. Universidad Autónoma de Bucaramanga. Rosendo Fernandez, J. and Pérez Zarauza, M., 2016. Guía Práctica de Úlceras de Pie Diabético. Santiago de Compostela: Programa Úlceras Fóra Sáenz Ramírez, A. (2004). Biomateriales. Tecnología En Marcha, 17(1), 34–45. Sarrigiannidis, S. O., Rey, J. M., Dobre, O., González-García, C., Dalby, M. J., & SalmeronSanchez, M. (2021). A tough act to follow: collagen hydrogel modifications to improve mechanical and growth factor loading capabilities. 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Biofilms in Diabetic Foot Ulcers: Impact, Risk Factors and Control Strategies. International Journal of Molecular Sciences, 22(15), 25. https://doi.org/10.3390/ijms22158278 |
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Solarte David, Víctor Alfonso54590e96-eda3-4b43-9ffa-14bd35ed7d08Becerra Bayona, Silvia Milenaf59fde3b-924f-4fcc-96e9-5fd6250b2daeRojas Cárdenas, Luis Davidbb7e0b38-6e76-4233-b16e-530f7693ebd6Solarte David, Víctor Alfonso [0001329391]Becerra Bayona, Silvia Milena [0001568861]Becerra Bayona, Silvia Milena [5wr21EQAAAAJ]Solarte David, Víctor Alfonso [0000-0002-9856-1484]Becerra Bayona, Silvia Milena [0000-0002-4499-5885]Becerra Bayona, Silvia Milena [36522328100]Solarte David, Víctor Alfonso [Victor-Solarte-David]Becerra Bayona, Silvia Milena [Silvia-Becerra-Bayona]Solarte David, Víctor Alfonso [víctor-alfonso-solarte-david]Becerra Bayona, Silvia Milena [silvia-milena-becerra-bayona]Becerra Bayona, Silvia Milena [silvia-becerra-3174455a]Bucaramanga (Santander, Colombia)2022UNAB Campus Bucaramanga2023-02-20T14:22:21Z2023-02-20T14:22:21Z2022http://hdl.handle.net/20.500.12749/19050instname:Universidad Autónoma de Bucaramanga - UNABreponame:Repositorio Institucional UNABrepourl:https://repository.unab.edu.coLa diabetes mellitus es una enfermedad que en la actualidad se considera un problema de salud pública y se estima que afecte a más de 700 millones de personas adultas en los años venideros. Una de sus principales complicaciones son las úlceras crónicas de pie diabético (UCPD), lesiones cutáneas que en la actualidad no tienen tratamientos 100% efectivos y que afectan de manera significativa la calidad de vida de quienes la padecen, lo que ha incrementado la necesidad de encontrar tratamientos que mejoren el proceso de cicatrización de este tipo de heridas. Por lo anterior, en el presente proyecto se ha sido utilizado piel de tilapia roja, un desecho de la industria acuícola, para extraer colágeno y con este elaborar hidrogeles, los cuales se reticularon con plasma pobre en plaquetas (PPP), una fracción de la sangre que no ha sido frecuentemente utilizada para la investigación. El proceso de extracción de colágeno ácido soluble permitió obtener un rendimiento en base seca cercano al 40%, y a partir de este, fabricar los hidrogeles y reticularlos con el PPP, obteniendo una dinámica adecuada en cuanto a la liberación de proteínas. Estos hidrogeles se sometieron a diferentes ensayos con el fin de determinar sus propiedades mecánicas y físicas, encontrando módulos de compresión similares a los de las capas internas de la piel, así como una capacidad de hinchamiento óptima, lo que les permitiría entregar la humedad necesaria a la herida durante el proceso de cicatrización. Finalmente, a partir de pruebas in vitro, se determinó que el lixiviado producido por los hidrogeles mantiene la viabilidad celular en un periodo de 48 horas, al permitir la proliferación de fibroblastos de la línea celular HT1080. Estos resultados indican que los hidrogeles fabricados en el presente estudio podrían ser una alternativa terapéutica para el tratamiento de las UCPD.Capítulo 1. Problemática identificada........................................................................................... 10 Planteamiento del problema ...................................................................................................... 10 Justificación............................................................................................................................... 11 Pregunta problema..................................................................................................................... 13 Objetivo general ........................................................................................................................ 13 Objetivos específicos................................................................................................................. 13 Capítulo 2. Marco teórico ............................................................................................................. 15 La piel........................................................................................................................................ 15 Propiedades mecánicas......................................................................................................... 16 Biomateriales............................................................................................................................. 17 El colágeno................................................................................................................................ 18 Estructura del colágeno......................................................................................................... 19 Propiedades y aplicaciones del colágeno ............................................................................. 20 Fuentes y proceso de extracción del colágeno...................................................................... 22 Plasma pobre en plaquetas (PPP).............................................................................................. 25 Hidrogeles ................................................................................................................................. 25 Úlceras crónicas de pie diabético (UCPD)................................................................................ 26 Tratamientos actuales para las UCPD ................................................................................. 28 Capítulo 3. Estado del arte ............................................................................................................ 29 Capítulo 4. Metodología ............................................................................................................... 33 Extracción de colágeno a partir de piel de tilapia roja .............................................................. 33 Preparación de la piel de tilapia roja ................................................................................... 34 Blanqueamiento de la piel ..................................................................................................... 34 Desengrasado de la piel ........................................................................................................ 34 Hidrólisis básica de la piel.................................................................................................... 35 Extracción ácida de la piel.................................................................................................... 35 Salting-out del precipitado .................................................................................................... 35 Diálisis del colágeno ............................................................................................................. 36 Liofilización del colágeno ..................................................................................................... 36 Rendimiento de extracción de colágeno ácido soluble (ASC)............................................... 36 Elaboración de hidrogeles......................................................................................................... 37 Hidrogeles de colágeno (HC)................................................................................................. 37 Hidrogeles de PPP y colágeno (HC+PPP)............................................................................... 38 Hidrogeles de PPP (HPPP)..................................................................................................... 38 Concentración de proteínas en los hidrogeles...................................................................... 39 Pruebas mecánicas..................................................................................................................... 40 Prueba mecánica de compresión (PMC)............................................................................... 40 Prueba mecánica de tensión (PMT)...................................................................................... 41 Prueba de hinchamiento........................................................................................................ 41 Prueba de degradación de los hidrogeles............................................................................. 42 Ensayo de citotoxicidad ............................................................................................................ 42 Prueba de proliferación celular con lixiviados de hidrogeles.............................................. 42 Análisis estadísticos.................................................................................................................. 45 Capítulo 5. Resultados y análisis.................................................................................................. 46 Extracción de colágeno a partir de piel de tilapia roja .............................................................. 46 Elaboración de hidrogeles de colágeno puro, PPP puro y colágeno + PPP .............................. 50 Concentración de proteínas inmovilizadas y liberadas por los hidrogeles................................ 52 Caracterización mecánica de los hidrogeles.............................................................................. 56 Evaluación del módulo de compresión.................................................................................. 56 Evaluación del módulo de Young .......................................................................................... 62 Evaluación de la capacidad de hinchamiento de los hidrogeles........................................... 63 Degradación de los hidrogeles.............................................................................................. 66 Ensayo de proliferación celular utilizando lixiviados de los hidrogeles................................... 69 Análisis de resultados................................................................................................................ 72 Capítulo 6. Conclusiones y recomendaciones .............................................................................. 82 Referencias.................................................................................................................................... 84 Anexos .......................................................................................................................................... 96PregradoDiabetes mellitus is a disease that is currently considered a public health problem and is estimated to affect more than 700 million adults in the coming years. One of its main complications are chronic diabetic foot ulcers, skin lesions that currently do not have 100% effective treatments and that significantly affect the quality of life of those who suffer it, which has increased the need to find treatments that improve the healing process of this type of wounds. Therefore, in the present project red tilapia skin, a waste from the aquaculture industry, has been used to extract collagen and with this to elaborate hydrogels, which were cross-linked with platelet-poor plasma (PPP), a fraction of the blood that has not been frequently used for research. The soluble acid collagen extraction process allowed to obtain a dry base yield close to 40%, and from this, to manufacture the hydrogels and cross-link them with the PPP, obtaining an adequate dynamic in terms of protein release. These hydrogels were subjected to different tests to determine their mechanical and physical properties, finding compression modules like those of the inner layers of the skin, as well as an optimal swelling capacity, which would allow them to deliver the necessary moisture to the wound during the healing process. Finally, from in vitro tests, it was determined that the leachate produced by the hydrogels maintains cell viability in a period of 48 hours, by allowing the proliferation of fibroblasts from the HT1080 cell line. These results indicate that the hydrogels manufactured in the present study could be a therapeutic alternative for the treatment of chronic diabetic foot ulcers.Modalidad Presencialapplication/pdfspahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/Abierto (Texto Completo)Atribución-NoComercial-SinDerivadas 2.5 Colombiahttp://purl.org/coar/access_right/c_abf2Desarrollo de un apósito tipo hidrogel de plasma pobre en plaquetas y colágeno extraído de piel de tilapia con potencial uso para el tratamiento de úlceras crónicas de pie diabéticoDevelopment of a platelet-poor plasma hydrogel-type dressing and collagen extracted from tilapia skin with potential use for the treatment of chronic diabetic foot ulcersIngeniero BiomédicoUniversidad Autónoma de Bucaramanga UNABFacultad IngenieríaPregrado Ingeniería Biomédicainfo:eu-repo/semantics/bachelorThesisTrabajo de Gradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/acceptedVersionhttp://purl.org/redcol/resource_type/TPBiomedical engineeringEngineeringMedical electronicsBiological physicsBioengineeringMedical instruments and apparatusMedicineBiomedicalClinical engineeringCollagenCytotoxicityFibroblastsHydrogelsPlatelet poor plasmaMellitus diabetesFoot diseasesFeet (Ulcers)Ingeniería biomédicaIngenieríaBiofísicaBioingenieríaMedicinaBiomédicaDiabetes mellitusEnfermedades de lo piesPies (Ulceras)Ingeniería clínicaElectrónica médicaInstrumentos y aparatos médicosColágenoCitotoxicidadFibroblastosHidrogelesPlasma pobre en plaquetasAfonso, A. 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