Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético

Actualmente los apósitos tipo hidrogel usados para el tratamiento de úlceras crónicas de pie diabético favorecen de manera limitada procesos de regeneración al ser apósitos bidimensionales, que sumado al potencial del PEGDA como biomaterial versátil en el campo de la ingeniería de tejidos, conviene...

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Autores:: Galeano Blanco, Marly Judith

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2021

Institución:: Universidad Autónoma de Bucaramanga - UNAB

Repositorio:: Repositorio UNAB

Idioma:: spa

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dc.title.spa.fl_str_mv	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético
dc.title.translated.spa.fl_str_mv	Methodological development of a pegda-based biomaterial ink, aimed at the design of dressings for chronic diabetic foot ulcers
title	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético
spellingShingle	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine 3D bioprinting Diacrylated polyethylene glycol Printability Ulcers Foot diseases Clinical engineering Diabetic foot Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Úlceras Enfermedades de los pies Pie diabético Ingeniería clínica Electrónica médica Instrumentos y aparatos médicos Biompresión 3D Polietilenglicol diacrilado Imprimibilidad
title_short	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético
title_full	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético
title_fullStr	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético
title_full_unstemmed	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético
title_sort	Desarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabético
dc.creator.fl_str_mv	Galeano Blanco, Marly Judith
dc.contributor.advisor.spa.fl_str_mv	Solarte David, Víctor Alfonso Becerra Bayona, Silvia Milena
dc.contributor.author.spa.fl_str_mv	Galeano Blanco, Marly Judith
dc.contributor.cvlac.*.fl_str_mv	https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001329391
dc.contributor.cvlac.none.fl_str_mv	Becerra Bayona, Silvia Milena [0001568861]
dc.contributor.googlescholar.none.fl_str_mv	Becerra Bayona, Silvia Milena [5wr21EQAAAAJ]
dc.contributor.orcid.*.fl_str_mv	https://orcid.org/0000-0002-9856-1484
dc.contributor.orcid.none.fl_str_mv	Becerra Bayona, Silvia Milena [0000-0002-4499-5885]
dc.contributor.scopus.none.fl_str_mv	Becerra Bayona, Silvia Milena [36522328100]
dc.contributor.researchgate.none.fl_str_mv	Becerra Bayona, Silvia Milena [Silvia-Becerra-Bayona]
dc.contributor.apolounab.none.fl_str_mv	Becerra Bayona, Silvia Milena [silvia-milena-becerra-bayona]
dc.contributor.linkedin.none.fl_str_mv	Becerra Bayona, Silvia Milena [silvia-becerra-3174455a]
dc.subject.keywords.eng.fl_str_mv	Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine 3D bioprinting Diacrylated polyethylene glycol Printability Ulcers Foot diseases Clinical engineering Diabetic foot
topic	Biomedical engineering Engineering Medical electronics Biological physics Bioengineering Medical instruments and apparatus Medicine 3D bioprinting Diacrylated polyethylene glycol Printability Ulcers Foot diseases Clinical engineering Diabetic foot Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Úlceras Enfermedades de los pies Pie diabético Ingeniería clínica Electrónica médica Instrumentos y aparatos médicos Biompresión 3D Polietilenglicol diacrilado Imprimibilidad
dc.subject.lemb.spa.fl_str_mv	Ingeniería biomédica Ingeniería Biofísica Bioingeniería Medicina Úlceras Enfermedades de los pies Pie diabético
dc.subject.proposal.spa.fl_str_mv	Ingeniería clínica Electrónica médica Instrumentos y aparatos médicos Biompresión 3D Polietilenglicol diacrilado Imprimibilidad
description	Actualmente los apósitos tipo hidrogel usados para el tratamiento de úlceras crónicas de pie diabético favorecen de manera limitada procesos de regeneración al ser apósitos bidimensionales, que sumado al potencial del PEGDA como biomaterial versátil en el campo de la ingeniería de tejidos, conviene desarrollar apósitos tridimensionales a base de PEGDA, lo que es posible mediante la bioimpresión 3D. De ahí que en esta investigación se evaluó la imprimibilidad de tinta de biomaterial a base de PEGDA usada en extrusión y fotopolimerización in-situ, con el fin de fabricar hidrogeles utilizando al Irgacure 2959 como fotoiniciador. Con este fin, se investigó el efecto que tiene la concentración de la solución precursora, además de los parámetros de bioimpresión tales como la velocidad de impresión, el flujo de material extruido y la altura de capa de impresión en la imprimibilidad. Sim embargo, pese al establecimiento de los mejores parámetros de bioimpresión: velocidad de impresión de 1 mm/s, altura de capa de 0.1 mm y flujo de 100%, los resultados demuestran que la viscosidad de la tinta de biomaterial a base de PEGDA requiere ser incrementada, para lograr la deposición de un filamento cilíndrico con una difusión de material mínima. Adicionalmente se llegó a la conclusión de que la intensidad de la luz ultravioleta de la bioimpresora debe ser mayor, para garantizar una polimerización eficiente y homogénea. La metodología desarrollada en este trabajo permite establecer las pautas de la fabricación de andamios a base de PEGDA, con el potencial uso en el diseño de matrices que lleven al desarrollo de alternativas terapéuticas tipo apósito, para el tratamiento de las úlceras crónicas de pie diabético, considerando la profundidad y la topografía de la úlcera.
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-03-16T18:42:22Z
dc.date.available.none.fl_str_mv	2021-03-16T18:42:22Z
dc.date.issued.none.fl_str_mv	2021
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/bachelorThesis
dc.type.local.spa.fl_str_mv	Trabajo de Grado
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_7a1f
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/TP
format	http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/20.500.12749/12431
dc.identifier.instname.spa.fl_str_mv	instname:Universidad Autónoma de Bucaramanga - UNAB
dc.identifier.reponame.spa.fl_str_mv	reponame:Repositorio Institucional UNAB
dc.identifier.repourl.spa.fl_str_mv	repourl:https://repository.unab.edu.co
url	http://hdl.handle.net/20.500.12749/12431
identifier_str_mv	instname:Universidad Autónoma de Bucaramanga - UNAB reponame:Repositorio Institucional UNAB repourl:https://repository.unab.edu.co
dc.language.iso.spa.fl_str_mv	spa
language	spa
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repository.name.fl_str_mv	Repositorio Institucional \| Universidad Autónoma de Bucaramanga - UNAB
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spelling	Solarte David, Víctor Alfonso54590e96-eda3-4b43-9ffa-14bd35ed7d08-1Becerra Bayona, Silvia Milenaf59fde3b-924f-4fcc-96e9-5fd6250b2dae-1Galeano Blanco, Marly Judith243f328b-7a64-4443-9c83-06f85b02d9dd-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001329391Becerra Bayona, Silvia Milena [0001568861]Becerra Bayona, Silvia Milena [5wr21EQAAAAJ]https://orcid.org/0000-0002-9856-1484Becerra Bayona, Silvia Milena [0000-0002-4499-5885]Becerra Bayona, Silvia Milena [36522328100]Becerra Bayona, Silvia Milena [Silvia-Becerra-Bayona]Becerra Bayona, Silvia Milena [silvia-milena-becerra-bayona]Becerra Bayona, Silvia Milena [silvia-becerra-3174455a]ColombiaUNAB Campus Bucaramanga2021-03-16T18:42:22Z2021-03-16T18:42:22Z2021http://hdl.handle.net/20.500.12749/12431instname:Universidad Autónoma de Bucaramanga - UNABreponame:Repositorio Institucional UNABrepourl:https://repository.unab.edu.coActualmente los apósitos tipo hidrogel usados para el tratamiento de úlceras crónicas de pie diabético favorecen de manera limitada procesos de regeneración al ser apósitos bidimensionales, que sumado al potencial del PEGDA como biomaterial versátil en el campo de la ingeniería de tejidos, conviene desarrollar apósitos tridimensionales a base de PEGDA, lo que es posible mediante la bioimpresión 3D. De ahí que en esta investigación se evaluó la imprimibilidad de tinta de biomaterial a base de PEGDA usada en extrusión y fotopolimerización in-situ, con el fin de fabricar hidrogeles utilizando al Irgacure 2959 como fotoiniciador. Con este fin, se investigó el efecto que tiene la concentración de la solución precursora, además de los parámetros de bioimpresión tales como la velocidad de impresión, el flujo de material extruido y la altura de capa de impresión en la imprimibilidad. Sim embargo, pese al establecimiento de los mejores parámetros de bioimpresión: velocidad de impresión de 1 mm/s, altura de capa de 0.1 mm y flujo de 100%, los resultados demuestran que la viscosidad de la tinta de biomaterial a base de PEGDA requiere ser incrementada, para lograr la deposición de un filamento cilíndrico con una difusión de material mínima. Adicionalmente se llegó a la conclusión de que la intensidad de la luz ultravioleta de la bioimpresora debe ser mayor, para garantizar una polimerización eficiente y homogénea. La metodología desarrollada en este trabajo permite establecer las pautas de la fabricación de andamios a base de PEGDA, con el potencial uso en el diseño de matrices que lleven al desarrollo de alternativas terapéuticas tipo apósito, para el tratamiento de las úlceras crónicas de pie diabético, considerando la profundidad y la topografía de la úlcera.Capítulo 1. Problema u Oportunidad ................................................................................. 8 Introducción .................................................................................................................... 8 Planteamiento del Problema ............................................................................................ 9 Justificación ................................................................................................................... 11 Pregunta Problema ........................................................................................................ 12 Objetivo General ........................................................................................................... 12 Objetivos Específicos .................................................................................................... 12 Limitaciones y Delimitaciones ...................................................................................... 13 Capítulo 2. Marco Teórico ................................................................................................ 14 Hidrogeles de PEDGA: Características del PEDGA y Polimerización ........................ 14 Bioimpresión: Tipos de Bioimpresión .......................................................................... 16 Parámetros de Biompresión en Extrusión 3D ........................................................... 17 Biotintas: Definición y su Distinción de Tinta de Biomaterial ..................................... 20 Requerimientos del Material de Bioimpresión.............................................................. 20 Capítulo 3. Estado del Arte ............................................................................................... 22 Aplicaciones en la Liberación de Fármacos .................................................................. 22 Aplicaciones en la Ingeniería de Tejidos ...................................................................... 23 Aplicaciones en el Área de Biosensado ........................................................................ 24 Aplicaciones del PEGDA en la Regeneración de Heridas ............................................ 25 Bioimpresión 3D e Hidrogeles PEGDA........................................................................ 26 Composición del Hidrogel de PEGDA ..................................................................... 26 Evaluación de la Impribilidad de Tintas de Biomaterial en Extrusión 3D ............... 28 Capítulo 4. Metodología ................................................................................................... 33 Preparación de las Soluciones Precursoras de PEGDA. ............................................... 33 Impresión de Hidrogeles de PEGDA ............................................................................ 33 Evaluación de la Morfología del Filamento .................................................................. 36 Evaluación de la Fidelidad de la Forma ........................................................................ 39 Análisis Estadísticos ...................................................................................................... 41 Capítulo 5. Resultados y Análisis de Resultados .............................................................. 43 Resultados ..................................................................................................................... 43 Impresión de Hidrogeles de PEGDA ........................................................................ 43 Evaluación de la Morfología del Filamento.............................................................. 50 Evaluación de la Fidelidad de la Forma .................................................................... 53 Análisis de Resultados .................................................................................................. 59 Capítulo 6. Conclusiones y Recomendaciones ................................................................. 63 Referencias ........................................................................................................................ 64PregradoCurrently the hydrogel-type dressings used for the treatment of chronic diabetic foot ulcers favor regeneration processes in a limited way as they are two-dimensional dressings, which added to the potential of PEGDA as a versatile biomaterial in the field of tissue engineering, it is convenient to develop three-dimensional dressings to PEGDA base, which is made possible by 3D bioprinting. Hence, in this research, the printability of PEGDA-based biomaterial ink used in extrusion and in-situ photopolymerization was evaluated, in order to manufacture hydrogels using Irgacure 2959 as a photoinitiator. To this end, the effect of precursor solution concentration, in addition to bioprinting parameters such as print speed, extrudate flow, and print layer height, on printability was investigated. However, despite the establishment of the best bioprinting parameters: printing speed of 1 mm / s, layer height of 0.1 mm and flow of 100%, the results show that the viscosity of the PEGDA-based biomaterial ink requires be increased, to achieve the deposition of a cylindrical filament with minimal material diffusion. Additionally, it was concluded that the intensity of the ultraviolet light from the bioprinter must be greater, to guarantee an efficient and homogeneous polymerization. The methodology developed in this work allows establishing the guidelines for the manufacture of PEGDA-based scaffolds, with the potential use in the design of matrices that lead to the development of dressing-type therapeutic alternatives for the treatment of chronic diabetic foot ulcers. considering the depth and topography of the ulcer.Modalidad Presencialapplication/pdfspahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/Abierto (Texto Completo)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 2.5 ColombiaDesarrollo metodológico de una tinta de biomaterial a base de pegda, dirigido al diseño de apósitos para úlceras crónicas de pie diabéticoMethodological development of a pegda-based biomaterial ink, aimed at the design of dressings for chronic diabetic foot ulcersIngeniero BiomédicoUniversidad Autónoma de Bucaramanga UNABFacultad IngenieríaPregrado Ingeniería Biomédicainfo:eu-repo/semantics/bachelorThesisTrabajo de Gradohttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/redcol/resource_type/TPBiomedical engineeringEngineeringMedical electronicsBiological physicsBioengineeringMedical instruments and apparatusMedicine3D bioprintingDiacrylated polyethylene glycolPrintabilityUlcersFoot diseasesClinical engineeringDiabetic footIngeniería biomédicaIngenieríaBiofísicaBioingenieríaMedicinaÚlcerasEnfermedades de los piesPie diabéticoIngeniería clínicaElectrónica médicaInstrumentos y aparatos médicosBiompresión 3DPolietilenglicol diacriladoImprimibilidadAycan, D., & Alemdar, N. 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